Rh2DG030500

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014898
Physical Location & Seq
Forward (+)
1872593 .. 1874637
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG030500.1

Sequence Viewer

Length: 852 bp
ATGGAGTCCAGTAAACTCAATGCTCTCTTCTTCATCTTCATGCTCGTCATTTCCTCGGCCGCTCCGATTCTCGGGTGCCCTACTTGCGGCAAAAAGCCTCCTCCCAAGCACACTAAGCCTAAGACCCCCAAGGGCCCTATTACAGTCCCTCCCATTGTGAAACCCCCAGTCATCAAAGTCCCCCCAGTCACAGTCCCACCTATCAAACCACCAGTGACAATACCACCCATCGTCAAACCACCAATCACCATCCCCCCCGTCATCCCTCCCATTGTCAAACCACCAATCACAATCCCACCAGTAATCCCTCCCGTCGTAAAACCACCAATTAAAATCCCACCCGTCATCCCTCCCATTGTCAAACCACCAATCACAATCCCACCAGTCATCCCTCCCGTCATAAAACCACCAATCAAAATCCCACCAGTGATTCCACCAATAGTGAAACCGCCAATCACATTACCACCAGTGGTAATCCCTCCAGTGATTCCCCCCATTGTCAAACCACCAGTGGAGATTCCCCCAATCACCACCAAGCCCCCGAGCCCCGGGACACCGTGCCCCCCACCACCTGGGACACCAGCTCCCAAGGACACTTGCCCCATCGACACACTGAAGCTCGGAGCTTGCGTGGATCTCCTCGGTGGGTTGGTTCACGTTGGGGAAGGTGACCCCGTGGTGAACGAGTGCTGCCCGGTGCTCTCAGGACTTGTTGAACTTGAAGCTGCAGTGTGCTTGTGCACCACCCTTAAACTCAAGCTTCTCAACCTCAACATCTTCGTCCCTATTGCTCTTGAGCTCCTTGTCACTTGTGGCAAGAGCCCTCCTCCTGGTTTCACCTGCTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

283

Amino Acids

29.41

Weight (kDa)

9.41

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 197 - 281 6e-06 Probable lipid transfer
Hydrophob_seed PF14547 199 - 282 1.6e-26 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 200 - 281 8.9e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G10940 AT2G10940
fragaria_vesca FvH4_1g02570
malus_domestica MD02G1023200.v1.1 MD15G1166100.v1.1
prunus_persica Prupe.7G248900_v2.0.a1 Prupe.7G248900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0087601
rosa_laevigata RLG00000015883
rosa_multiflora Rmu_sc0002299.1_g000028
rosa_rugosa Rorug01G0476900
rosa_samantha Rh2AG030500 Rh2BG030300 Rh2CG030700 Rh2DG030500
rosa_wichuraiana Rw2G002420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 848
Acc36I ACCTGC 1 cut(s) 848
AccB1I GGYRCC 1 cut(s) 75
AccB7I CCANNNNNTGG 1 cut(s) 572
AccBSI CCGCTC 1 cut(s) 62
AciI CCGC 3 cut(s) 60, 87, 449
AclWI GGATC 1 cut(s) 642
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 635
AfiI CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
AgsI TTSAA 2 cut(s) 716, 722
AjnI CCWGG 2 cut(s) 571, 829
AluBI AGCT 6 cut(s) 584, 619, 626, 725, 760, 799
AluI AGCT 6 cut(s) 584, 619, 626, 725, 760, 799
Alw21I GWGCWC 3 cut(s) 702, 743, 801
Alw44I GTGCAC 1 cut(s) 739
AlwI GGATC 1 cut(s) 642
Ama87I CYCGRG 3 cut(s) 71, 541, 548
AoxI GGCC 2 cut(s) 57, 133
ApaI GGGCCC 1 cut(s) 137
ApaLI GTGCAC 1 cut(s) 739
ApeKI GCWGC 2 cut(s) 690, 725
AspS9I GGNCC 2 cut(s) 133, 134
AsuC2I CCSGG 3 cut(s) 549, 550, 695
AsuHPI GGTGA 5 cut(s) 238, 520, 680, 691, 829
AvaI CYCGRG 3 cut(s) 71, 541, 548
BaeGI GKGCMC 4 cut(s) 80, 137, 563, 743
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 4 cut(s) 137, 548, 801, 824
Bbv12I GWGCWC 3 cut(s) 702, 743, 801
BbvI GCAGC 2 cut(s) 677, 712
BccI CCATC 3 cut(s) 236, 257, 611
BciT130I CCWGG 2 cut(s) 573, 831
BcnI CCSGG 3 cut(s) 549, 550, 695
BfaI CTAG 1 cut(s) 850
BfmI CTRYAG 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 848
BisI GCNGC 4 cut(s) 60, 88, 691, 726
BlsI GCNGC 4 cut(s) 61, 89, 692, 727
Bme1390I CCNGG 5 cut(s) 549, 550, 573, 695, 831
BmeT110I CYCGRG 3 cut(s) 71, 541, 548
BmgT120I GGNCC 2 cut(s) 133, 134
BmiI GGNNCC 2 cut(s) 77, 135
BmrFI CCNGG 5 cut(s) 549, 550, 573, 695, 831
BmrI ACTGGG 2 cut(s) 161, 179
BmuI ACTGGG 2 cut(s) 161, 179
BplI GAGNNNNNCTC 2 cut(s) 811, 843
BpmI CTGGAG 1 cut(s) 465
BpuEI CTTGAG 2 cut(s) 740, 815
BpuMI CCSGG 3 cut(s) 549, 550, 695
BsaJI CCNNGG 8 cut(s) 54, 129, 547, 548, 572, 588, 640, 675
BsaXI ACNNNNNCTCC 6 cut(s) 133, 163, 568, 598, 615, 645
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
BseBI CCWGG 2 cut(s) 573, 831
BseDI CCNNGG 8 cut(s) 54, 129, 547, 548, 572, 588, 640, 675
BseGI GGATG 4 cut(s) 249, 261, 345, 387
BseLI CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
BseMII CTCAG 1 cut(s) 717
BseRI GAGGAG 3 cut(s) 90, 629, 816
BseSI GKGCMC 4 cut(s) 80, 137, 563, 743
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 677, 712
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 135
BshNI GGYRCC 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 3 cut(s) 702, 743, 801
BsiHKCI CYCGRG 3 cut(s) 71, 541, 548
BsiSI CCGG 2 cut(s) 549, 695
BslFI GGGAC 6 cut(s) 131, 164, 179, 565, 589, 767
BslI CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
BsmFI GGGAC 6 cut(s) 131, 164, 179, 565, 589, 767
BsnI GGCC 2 cut(s) 59, 135
BsoBI CYCGRG 3 cut(s) 71, 541, 548
Bsp120I GGGCCC 1 cut(s) 133
Bsp1286I GDGCHC 8 cut(s) 80, 137, 548, 563, 702, 743, 801, 824
Bsp143I GATC 1 cut(s) 634
BspACI CCGC 3 cut(s) 60, 87, 449
BspANI GGCC 2 cut(s) 59, 135
BspCNI CTCAG 1 cut(s) 716
BspLI GGNNCC 2 cut(s) 77, 135
BspMAI CTGCAG 1 cut(s) 730
BspMI ACCTGC 1 cut(s) 848
BspPI GGATC 1 cut(s) 642
BspT107I GGYRCC 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 62
BssECI CCNNGG 8 cut(s) 54, 129, 547, 548, 572, 588, 640, 675
BssMI GATC 1 cut(s) 634
BssT1I CCWWGG 2 cut(s) 129, 588
Bst2UI CCWGG 2 cut(s) 573, 831
Bst4CI ACNGT 3 cut(s) 145, 193, 558
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 3 cut(s) 114, 120, 703
BstDSI CCRYGG 1 cut(s) 675
BstEII GGTNACC 1 cut(s) 668
BstF5I GGATG 4 cut(s) 249, 261, 345, 387
BstKTI GATC 1 cut(s) 637
BstMBI GATC 1 cut(s) 634
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 84, 115
BstNI CCWGG 2 cut(s) 573, 831
BstPI GGTNACC 1 cut(s) 668
BstSCI CCNGG 5 cut(s) 547, 548, 571, 693, 829
BstSFI CTRYAG 1 cut(s) 726
BstSLI GKGCMC 4 cut(s) 80, 137, 563, 743
BstV1I GCAGC 2 cut(s) 677, 712
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 135
BtgI CCRYGG 1 cut(s) 675
BtsCI GGATG 4 cut(s) 249, 261, 345, 387
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 7 cut(s) 219, 432, 474, 489, 516, 611, 735
BveI ACCTGC 1 cut(s) 848
Cac8I GCNNGC 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 133, 134
Cfr9I CCCGGG 1 cut(s) 548
CviAII CATG 1 cut(s) 40
DdeI CTNAG 3 cut(s) 114, 120, 703
DpnI GATC 1 cut(s) 636
DpnII GATC 1 cut(s) 634
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 799
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 129, 588
Eco24I GRGCYC 4 cut(s) 137, 548, 801, 824
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 1 cut(s) 635
Eco88I CYCGRG 3 cut(s) 71, 541, 548
Eco91I GGTNACC 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 799
EcoO109I RGGNCCY 2 cut(s) 133, 134
EcoO65I GGTNACC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 571, 829
EcoT14I CCWWGG 2 cut(s) 129, 588
EcoT38I GRGCYC 4 cut(s) 137, 548, 801, 824
ErhI CCWWGG 2 cut(s) 129, 588
FaeI CATG 1 cut(s) 43
FaiI YATR 2 cut(s) 41, 401
FaqI GGGAC 6 cut(s) 131, 164, 179, 565, 589, 767
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 60, 88, 691, 726
FokI GGATG 4 cut(s) 236, 248, 332, 374
FriOI GRGCYC 4 cut(s) 137, 548, 801, 824
Fsp4HI GCNGC 4 cut(s) 60, 88, 691, 726
FspBI CTAG 1 cut(s) 850
GluI GCNGC 4 cut(s) 60, 88, 691, 726
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 2 cut(s) 59, 135
HapII CCGG 2 cut(s) 549, 695
Hin1II CATG 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 5 cut(s) 5, 67, 430, 487, 517
HpaII CCGG 2 cut(s) 549, 695
HphI GGTGA 5 cut(s) 238, 520, 680, 691, 829
Hpy166II GTNNAC 4 cut(s) 14, 655, 682, 741
Hpy188I TCNGA 2 cut(s) 66, 623
Hpy188III TCNNGA 2 cut(s) 705, 794
Hpy8I GTNNAC 4 cut(s) 14, 655, 682, 741
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 1 cut(s) 659
HpyCH4III ACNGT 3 cut(s) 145, 193, 558
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 2 cut(s) 728, 741
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 115
HpyF3I CTNAG 3 cut(s) 114, 120, 703
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 1 cut(s) 43
Kzo9I GATC 1 cut(s) 634
LmnI GCTCC 4 cut(s) 67, 589, 623, 804
Lsp1109I GCAGC 2 cut(s) 677, 712
MaeI CTAG 1 cut(s) 850
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 4 cut(s) 187, 214, 668, 805
MalI GATC 1 cut(s) 636
MbiI CCGCTC 1 cut(s) 62
MboI GATC 1 cut(s) 634
MboII GAAGA 4 cut(s) 19, 22, 28, 769
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 8 cut(s) 80, 137, 548, 563, 702, 743, 801, 824
MluCI AATT 1 cut(s) 327
MlyI GAGTC 1 cut(s) 14
MseI TTAA 2 cut(s) 330, 750
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 2 cut(s) 549, 695
MspR9I CCNGG 5 cut(s) 549, 550, 573, 695, 831
MvaI CCWGG 2 cut(s) 573, 831
MwoI GCNNNNNNNGC 2 cut(s) 84, 115
NciI CCSGG 3 cut(s) 549, 550, 695
NdeII GATC 1 cut(s) 634
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 2 cut(s) 77, 135
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 4 cut(s) 187, 214, 668, 805
PaqCI CACCTGC 1 cut(s) 848
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 627
PfeI GAWTC 4 cut(s) 67, 430, 487, 517
PflMI CCANNNNNTGG 1 cut(s) 572
PkrI GCNGC 4 cut(s) 61, 89, 692, 727
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 801
Psp6I CCWGG 2 cut(s) 571, 829
PspEI GGTNACC 1 cut(s) 668
PspGI CCWGG 2 cut(s) 571, 829
PspN4I GGNNCC 2 cut(s) 77, 135
PspOMI GGGCCC 1 cut(s) 133
PspPI GGNCC 2 cut(s) 133, 134
PstI CTGCAG 1 cut(s) 730
PsuI RGATCY 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 801
SaqAI TTAA 2 cut(s) 330, 750
SatI GCNGC 4 cut(s) 60, 88, 691, 726
Sau3AI GATC 1 cut(s) 634
Sau96I GGNCC 2 cut(s) 133, 134
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 5 cut(s) 549, 550, 573, 695, 831
SduI GDGCHC 8 cut(s) 80, 137, 548, 563, 702, 743, 801, 824
SfcI CTRYAG 1 cut(s) 726
SmaI CCCGGG 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 755, 794
SmoI CTYRAG 2 cut(s) 755, 794
Sse9I AATT 1 cut(s) 327
SsiI CCGC 3 cut(s) 60, 87, 449
SspMI CTAG 1 cut(s) 850
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 5 cut(s) 547, 548, 571, 693, 829
StyI CCWWGG 2 cut(s) 129, 588
TaaI ACNGT 3 cut(s) 145, 193, 558
TaiI ACGT 1 cut(s) 660
TaqI TCGA 1 cut(s) 606
TasI AATT 1 cut(s) 327
TauI GCSGC 2 cut(s) 62, 90
TfiI GAWTC 4 cut(s) 67, 430, 487, 517
Tru1I TTAA 2 cut(s) 330, 750
Tru9I TTAA 2 cut(s) 330, 750
TscAI CASTG 7 cut(s) 219, 432, 474, 489, 516, 618, 735
TseFI GTSAC 4 cut(s) 187, 214, 668, 805
TseI GCWGC 2 cut(s) 690, 725
Tsp45I GTSAC 4 cut(s) 187, 214, 668, 805
TspDTI ATGAA 2 cut(s) 22, 28
TspMI CCCGGG 1 cut(s) 548
TspRI CASTG 7 cut(s) 219, 432, 474, 489, 516, 618, 735
Van91I CCANNNNNTGG 1 cut(s) 572
VneI GTGCAC 1 cut(s) 739
XmaI CCCGGG 1 cut(s) 548
XspI CTAG 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.