FvH4_1g06650

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016233
Physical Location & Seq
Forward (+)
3515865 .. 3517011
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06650.t1

Sequence Viewer

Length: 843 bp
ATGAGACCAGGATCAGCAGATGCAACCCAACCAGCCAATCCCCGGTGGGGAGATCTCGACGAAGAGGACGGTGACAAGGAGTTGGACCTCCTCCTTCTGCCGCCGAAGCAGGTGATCGGTCCCAACGAGAAGGGTATCAAGAAAGTGATTGAATACAAGTTCAATGAAGAAGGCCACAAGGTCAAGATCACCACCACCACCCGGATCCAATCTCGCCAAATGACTAAAGCCGCCGTTGAGCGCCGCTCATGGGCCAAGTTCGGAGATGCTGTGGATGAAGACGCCGGTGCCAACTTGACTGTGGTCTCCAAAGACGAGATCATGCTCGAACGCCCTAAGGCTCTTGGCACCAACACAGCACATGAACTACCAAAGACTAATGAAGGAGGCAATACTACTCTCAAGCTTTGTAGGACTTGTGGTAAGAAAGGTGACCACTGGACTGCACAGTGCCTTTACAAAGACCTTGCCACACAAAGTACCGGCGATATTAGCTCATCGGAGGCGGGCACAACTAGCAAAACCGGTACATATGTTCCTCCGGGCATGAGACCAGGAGGGAAGAAGAGTACTCCGAGGAATGATGAGAATTCTGTTCGGGTTAGCAACCTTTCGGAGGATGCCCGGGATGCCGATTTGTATGAACTCTTTAGTCCTTTTGGTGATATTAGCCGTGCTCATGTTGTTCTTGAGAGAGAGACACAAGTTAGCAGAGGATTTGGTTTTGTTAACTTTGTGAACAAGGAACATGGCCGAAGAGCTATAGACAAACTCAATGGGTATGGCTATGACAACCTCATTCTCCGGGTGGAATGGGCCACCCCCAGGCTCAGGCCCAATTAA

Protein Analysis

281

Amino Acids

31.02

Weight (kDa)

7.69

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 37 - 156 5.7e-30 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 199 - 265 4.6e-18 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 100
AccB1I GGYRCC 2 cut(s) 287, 347
AccBSI CCGCTC 1 cut(s) 246
AciI CCGC 4 cut(s) 101, 231, 244, 506
AclWI GGATC 3 cut(s) 19, 199, 212
AcoI YGGCCR 1 cut(s) 751
AcsI RAATTY 1 cut(s) 589
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 3 cut(s) 481, 529, 571
AfiI CCNNNNNNNGG 7 cut(s) 42, 47, 201, 250, 616, 825, 831
AgeI ACCGGT 1 cut(s) 524
AgsI TTSAA 2 cut(s) 152, 163
AjnI CCWGG 3 cut(s) 7, 553, 824
AluBI AGCT 3 cut(s) 406, 495, 761
AluI AGCT 3 cut(s) 406, 495, 761
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 3 cut(s) 310, 544, 692
AlwI GGATC 3 cut(s) 19, 199, 212
Ama87I CYCGRG 1 cut(s) 624
AoxI GGCC 5 cut(s) 172, 252, 751, 816, 833
ApoI RAATTY 1 cut(s) 589
AsiGI ACCGGT 1 cut(s) 524
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 5 cut(s) 85, 119, 252, 816, 834
AsuC2I CCSGG 6 cut(s) 43, 202, 543, 625, 626, 806
AsuHPI GGTGA 5 cut(s) 83, 124, 181, 443, 674
AvaI CYCGRG 1 cut(s) 624
AvaII GGWCC 2 cut(s) 85, 119
AxyI CCTNAGG 1 cut(s) 336
BaeGI GKGCMC 1 cut(s) 512
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BamHI GGATCC 1 cut(s) 204
BanI GGYRCC 2 cut(s) 287, 347
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 679
BceAI ACGGC 2 cut(s) 218, 657
BciT130I CCWGG 3 cut(s) 9, 555, 826
BcnI CCSGG 6 cut(s) 43, 202, 543, 625, 626, 806
BcoDI GTCTC 3 cut(s) 310, 544, 692
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 1 cut(s) 244
BfuAI ACCTGC 1 cut(s) 100
BglII AGATCT 1 cut(s) 52
BisI GCNGC 3 cut(s) 101, 231, 244
BlsI GCNGC 3 cut(s) 102, 232, 245
BmcAI AGTACT 1 cut(s) 571
Bme1390I CCNGG 9 cut(s) 9, 43, 202, 543, 555, 625, 626, 806, 826
Bme18I GGWCC 2 cut(s) 85, 119
BmeT110I CYCGRG 1 cut(s) 624
BmgT120I GGNCC 5 cut(s) 85, 119, 252, 816, 834
BmiI GGNNCC 4 cut(s) 121, 206, 289, 349
BmrFI CCNGG 9 cut(s) 9, 43, 202, 543, 555, 625, 626, 806, 826
BmsI GCATC 4 cut(s) 10, 256, 610, 619
BoxI GACNNNNGTC 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 285
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu10I CCTNAGC 1 cut(s) 830
BpuEI CTTGAG 2 cut(s) 386, 710
BpuMI CCSGG 6 cut(s) 43, 202, 543, 625, 626, 806
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 2 cut(s) 310, 544
BsaJI CCNNGG 4 cut(s) 41, 575, 624, 824
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 7 cut(s) 42, 47, 201, 250, 616, 825, 831
Bse118I RCCGGY 3 cut(s) 284, 482, 524
Bse1I ACTGG 1 cut(s) 443
Bse21I CCTNAGG 1 cut(s) 336
BseBI CCWGG 3 cut(s) 9, 555, 826
BseDI CCNNGG 4 cut(s) 41, 575, 624, 824
BseGI GGATG 3 cut(s) 280, 625, 634
BseLI CCNNNNNNNGG 7 cut(s) 42, 47, 201, 250, 616, 825, 831
BseNI ACTGG 1 cut(s) 443
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 512
BsgI GTGCAG 1 cut(s) 429
BshFI GGCC 5 cut(s) 174, 254, 753, 818, 835
BshNI GGYRCC 2 cut(s) 287, 347
BshTI ACCGGT 1 cut(s) 524
BsiHKAI GWGCWC 1 cut(s) 679
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 8 cut(s) 43, 202, 285, 483, 525, 542, 625, 805
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 7 cut(s) 42, 47, 201, 250, 616, 825, 831
BsmAI GTCTC 3 cut(s) 310, 544, 692
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 5 cut(s) 174, 254, 753, 818, 835
Bso31I GGTCTC 2 cut(s) 310, 544
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 512, 679
Bsp143I GATC 6 cut(s) 11, 52, 114, 186, 204, 318
BspACI CCGC 4 cut(s) 101, 231, 244, 506
BspANI GGCC 5 cut(s) 174, 254, 753, 818, 835
BspCNI CTCAG 1 cut(s) 843
BspLI GGNNCC 4 cut(s) 121, 206, 289, 349
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 3 cut(s) 19, 199, 212
BspQI GCTCTTC 1 cut(s) 751
BspT107I GGYRCC 2 cut(s) 287, 347
BspTNI GGTCTC 2 cut(s) 310, 544
BsrBI CCGCTC 1 cut(s) 246
BsrFI RCCGGY 3 cut(s) 284, 482, 524
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 3 cut(s) 284, 482, 524
BssECI CCNNGG 4 cut(s) 41, 575, 624, 824
BssMI GATC 6 cut(s) 11, 52, 114, 186, 204, 318
BssNI GRCGYC 1 cut(s) 282
Bst2UI CCWGG 3 cut(s) 9, 555, 826
Bst4CI ACNGT 3 cut(s) 71, 301, 450
Bst6I CTCTTC 3 cut(s) 57, 560, 751
BstACI GRCGYC 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 2 cut(s) 336, 830
BstEII GGTNACC 1 cut(s) 431
BstENI CCTNNNNNAGG 1 cut(s) 614
BstF5I GGATG 3 cut(s) 280, 625, 634
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 6 cut(s) 14, 55, 117, 189, 207, 321
BstMAI GTCTC 3 cut(s) 310, 544, 692
BstMBI GATC 6 cut(s) 11, 52, 114, 186, 204, 318
BstMWI GCNNNNNNNGC 4 cut(s) 106, 492, 516, 629
BstNI CCWGG 3 cut(s) 9, 555, 826
BstPAI GACNNNNGTC 1 cut(s) 302
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 9 cut(s) 7, 41, 200, 541, 553, 623, 624, 804, 824
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 2 cut(s) 52, 204
BstYI RGATCY 2 cut(s) 52, 204
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 5 cut(s) 174, 254, 753, 818, 835
BtsCI GGATG 3 cut(s) 280, 625, 634
BtsIMutI CAGTG 2 cut(s) 436, 455
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 508
CfoI GCGC 1 cut(s) 243
Cfr10I RCCGGY 3 cut(s) 284, 482, 524
Cfr13I GGNCC 5 cut(s) 85, 119, 252, 816, 834
Cfr9I CCCGGG 1 cut(s) 624
CseI GACGC 1 cut(s) 290
Csp6I GTAC 3 cut(s) 480, 528, 570
CspAI ACCGGT 1 cut(s) 524
CviAII CATG 6 cut(s) 249, 322, 362, 547, 680, 749
CviQI GTAC 3 cut(s) 480, 528, 570
DdeI CTNAG 2 cut(s) 336, 830
DpnI GATC 6 cut(s) 13, 54, 116, 188, 206, 320
DpnII GATC 6 cut(s) 11, 52, 114, 186, 204, 318
EaeI YGGCCR 1 cut(s) 751
Eam1104I CTCTTC 3 cut(s) 57, 560, 751
EarI CTCTTC 3 cut(s) 57, 560, 751
Eco31I GGTCTC 2 cut(s) 310, 544
Eco47I GGWCC 2 cut(s) 85, 119
Eco81I CCTNAGG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 624
Eco91I GGTNACC 1 cut(s) 431
EcoNI CCTNNNNNAGG 1 cut(s) 614
EcoO65I GGTNACC 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 589
EcoRII CCWGG 3 cut(s) 7, 553, 824
FaeI CATG 6 cut(s) 252, 325, 365, 550, 683, 752
FaqI GGGAC 1 cut(s) 105
FatI CATG 6 cut(s) 248, 321, 361, 546, 679, 748
FauI CCCGC 1 cut(s) 499
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 101, 231, 244
FokI GGATG 3 cut(s) 287, 632, 641
Fsp4HI GCNGC 3 cut(s) 101, 231, 244
FspBI CTAG 1 cut(s) 516
GlaI GCGC 1 cut(s) 242
GluI GCNGC 3 cut(s) 101, 231, 244
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 5 cut(s) 174, 254, 753, 818, 835
HapII CCGG 8 cut(s) 43, 202, 285, 483, 525, 542, 625, 805
HgaI GACGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 243
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 6 cut(s) 252, 325, 365, 550, 683, 752
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 1 cut(s) 404
HpaI GTTAAC 1 cut(s) 730
HpaII CCGG 8 cut(s) 43, 202, 285, 483, 525, 542, 625, 805
HphI GGTGA 5 cut(s) 83, 124, 181, 443, 674
Hpy166II GTNNAC 2 cut(s) 730, 739
Hpy188I TCNGA 4 cut(s) 263, 502, 576, 616
Hpy188III TCNNGA 4 cut(s) 56, 139, 184, 689
Hpy8I GTNNAC 2 cut(s) 730, 739
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 4 cut(s) 104, 124, 164, 377
HpyCH4III ACNGT 3 cut(s) 71, 301, 450
HpyCH4V TGCA 2 cut(s) 23, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 492, 516, 629
HpyF3I CTNAG 2 cut(s) 336, 830
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 6 cut(s) 252, 325, 365, 550, 683, 752
HspAI GCGC 1 cut(s) 241
KspAI GTTAAC 1 cut(s) 730
Kzo9I GATC 6 cut(s) 11, 52, 114, 186, 204, 318
LguI GCTCTTC 1 cut(s) 751
LweI GCATC 4 cut(s) 10, 256, 610, 619
MaeI CTAG 1 cut(s) 516
MaeIII GTNAC 2 cut(s) 71, 431
MalI GATC 6 cut(s) 13, 54, 116, 188, 206, 320
MbiI CCGCTC 1 cut(s) 246
MboI GATC 6 cut(s) 11, 52, 114, 186, 204, 318
MboII GAAGA 6 cut(s) 74, 179, 290, 574, 577, 768
MflI RGATCY 2 cut(s) 52, 204
MhlI GDGCHC 2 cut(s) 512, 679
MluCI AATT 2 cut(s) 589, 838
MmeI TCCRAC 1 cut(s) 63
MseI TTAA 2 cut(s) 729, 841
MspI CCGG 8 cut(s) 43, 202, 285, 483, 525, 542, 625, 805
MspR9I CCNGG 9 cut(s) 9, 43, 202, 543, 555, 625, 626, 806, 826
MvaI CCWGG 3 cut(s) 9, 555, 826
MwoI GCNNNNNNNGC 4 cut(s) 106, 492, 516, 629
NciI CCSGG 6 cut(s) 43, 202, 543, 625, 626, 806
NdeI CATATG 1 cut(s) 532
NdeII GATC 6 cut(s) 11, 52, 114, 186, 204, 318
NlaIII CATG 6 cut(s) 252, 325, 365, 550, 683, 752
NlaIV GGNNCC 4 cut(s) 121, 206, 289, 349
NmuCI GTSAC 2 cut(s) 71, 431
PaqCI CACCTGC 1 cut(s) 100
PciSI GCTCTTC 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PinAI ACCGGT 1 cut(s) 524
PkrI GCNGC 3 cut(s) 102, 232, 245
PshAI GACNNNNGTC 1 cut(s) 302
Psp6I CCWGG 3 cut(s) 7, 553, 824
PspEI GGTNACC 1 cut(s) 431
PspGI CCWGG 3 cut(s) 7, 553, 824
PspN4I GGNNCC 4 cut(s) 121, 206, 289, 349
PspPI GGNCC 5 cut(s) 85, 119, 252, 816, 834
PsuI RGATCY 2 cut(s) 52, 204
RsaI GTAC 3 cut(s) 481, 529, 571
RsaNI GTAC 3 cut(s) 480, 528, 570
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 2 cut(s) 729, 841
SatI GCNGC 3 cut(s) 101, 231, 244
Sau3AI GATC 6 cut(s) 11, 52, 114, 186, 204, 318
Sau96I GGNCC 5 cut(s) 85, 119, 252, 816, 834
ScaI AGTACT 1 cut(s) 571
ScrFI CCNGG 9 cut(s) 9, 43, 202, 543, 555, 625, 626, 806, 826
SduI GDGCHC 2 cut(s) 512, 679
SfaNI GCATC 4 cut(s) 10, 256, 610, 619
SfcI CTRYAG 1 cut(s) 762
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 2 cut(s) 85, 119
SmaI CCCGGG 1 cut(s) 626
SmlI CTYRAG 2 cut(s) 401, 689
SmoI CTYRAG 2 cut(s) 401, 689
Sse9I AATT 2 cut(s) 589, 838
SsiI CCGC 4 cut(s) 101, 231, 244, 506
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 9 cut(s) 7, 41, 200, 541, 553, 623, 624, 804, 824
TaaI ACNGT 3 cut(s) 71, 301, 450
TaqI TCGA 2 cut(s) 57, 327
TaqII GACCGA 1 cut(s) 107
TasI AATT 2 cut(s) 589, 838
TatI WGTACW 1 cut(s) 569
TauI GCSGC 3 cut(s) 103, 233, 246
Tru1I TTAA 2 cut(s) 729, 841
Tru9I TTAA 2 cut(s) 729, 841
TscAI CASTG 2 cut(s) 443, 455
TseFI GTSAC 2 cut(s) 71, 431
Tsp45I GTSAC 2 cut(s) 71, 431
TspDTI ATGAA 5 cut(s) 180, 291, 378, 396, 657
TspMI CCCGGG 1 cut(s) 624
TspRI CASTG 2 cut(s) 443, 455
VpaK11BI GGWCC 2 cut(s) 85, 119
XagI CCTNNNNNAGG 1 cut(s) 614
XapI RAATTY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 624
XspI CTAG 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.