Rmu_co8481273.1_g000001

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8481273.1
OG0016233
Physical Location & Seq
Reverse (-)
313 .. 1161
849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8481273.1_g000001.1.cds

Sequence Viewer

Length: 849 bp
atgagaccaggatcgggtgatgctacccaaccggccaagccccggtgggcagatatcgatgaggaggacggacaggaggagttggacgtcctccttttgccgccaaagcaggtgattggccccgacgagaaggggatggagaaggtgattgaatacaaattcaatgaaaagggtcagaaggtcaagatcaccaccaccaccaggaccaaatctcacctcatgagtaaggcggcagtggagcggcgctcctggccaaaatttggtggtgccgtcaatgaagacgccggtgccaatctgacagcggtttccaaagaggagatcatgctcgaatgccccaaggctcttggcgccaacaaggcacaagaactaccaaagagtactagtggtaatggaagaggcaatactactctcaaggtttgtaggacttgtggcaagaaaggtgaccactggactgcacagtgcccatacaaagatcttgccccgccgagttccgaagatattagctcatccgaggcagccacaactagcaagattggtacatacgttcctccgggtttgagagaaggagtgaagagggctctcaggaatgatgaaaattcagttcgggtgagcaacctttcggaggatacccgggaccccgatttgcatgaacttttcagtccttttggtgccataagccgtgctcatgttgttcttgagagagacacccaagttagcagaggatttggttttgttgactttctcaacaaggaacacggccaaagagccatagacaaactcaatgggtatggttatgacaatctcatccttcgggtagaatgggccacgccccggcccaggcccaattga

Protein Analysis

282

Amino Acids

31.07

Weight (kDa)

7.66

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
AatII GACGTC 1 cut(s) 90
Acc36I ACCTGC 1 cut(s) 100
AccB1I GGYRCC 4 cut(s) 266, 287, 347, 668
AccB7I CCANNNNNTGG 1 cut(s) 260
AccBSI CCGCTC 1 cut(s) 241
AciI CCGC 5 cut(s) 101, 230, 241, 302, 482
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 3 cut(s) 33, 251, 757
AcsI RAATTY 3 cut(s) 158, 257, 595
AcyI GRCGYC 3 cut(s) 87, 282, 348
AfaI GTAC 2 cut(s) 379, 538
AfiI CCNNNNNNNGG 7 cut(s) 14, 42, 201, 260, 622, 831, 837
AgsI TTSAA 2 cut(s) 152, 163
AhlI ACTAGT 1 cut(s) 380
AjnI CCWGG 4 cut(s) 7, 200, 248, 836
AluBI AGCT 1 cut(s) 504
AluI AGCT 1 cut(s) 504
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 1 cut(s) 696
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 7 cut(s) 33, 118, 251, 757, 822, 833, 839
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 3 cut(s) 158, 257, 595
AspLEI GCGC 2 cut(s) 246, 350
AspS9I GGNCC 6 cut(s) 119, 204, 634, 822, 834, 840
AsuC2I CCSGG 5 cut(s) 43, 552, 631, 632, 832
AsuHPI GGTGA 7 cut(s) 29, 124, 157, 181, 206, 452, 619
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 2 cut(s) 204, 634
BaeGI GKGCMC 1 cut(s) 464
BaeI ACNNNNGTAYC 2 cut(s) 528, 561
BalI TGGCCA 1 cut(s) 253
BanI GGYRCC 4 cut(s) 266, 287, 347, 668
BanII GRGCYC 1 cut(s) 580
BarI GAAGNNNNNNTAC 2 cut(s) 385, 417
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 1 cut(s) 527
BccI CCATC 1 cut(s) 130
BceAI ACGGC 3 cut(s) 254, 663, 772
BciT130I CCWGG 4 cut(s) 9, 202, 250, 838
BciVI GTATCC 1 cut(s) 619
BcnI CCSGG 5 cut(s) 43, 552, 631, 632, 832
BcoDI GTCTC 1 cut(s) 696
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 2 cut(s) 381, 525
BfoI RGCGCY 2 cut(s) 247, 351
BfuAI ACCTGC 1 cut(s) 100
BfuI GTATCC 1 cut(s) 619
BglI GCCNNNNNGGC 1 cut(s) 356
BglII AGATCT 1 cut(s) 472
BisI GCNGC 4 cut(s) 101, 231, 242, 516
BlsI GCNGC 4 cut(s) 102, 232, 243, 517
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 9 cut(s) 9, 43, 202, 250, 552, 631, 632, 832, 838
Bme18I GGWCC 2 cut(s) 204, 634
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 6 cut(s) 119, 204, 634, 822, 834, 840
BmiI GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
BmrFI CCNGG 9 cut(s) 9, 43, 202, 250, 552, 631, 632, 832, 838
BmsI GCATC 1 cut(s) 10
BpiI GAAGAC 1 cut(s) 285
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpuEI CTTGAG 2 cut(s) 395, 716
BpuMI CCSGG 5 cut(s) 43, 552, 631, 632, 832
Bsa29I ATCGAT 1 cut(s) 57
BsaHI GRCGYC 3 cut(s) 87, 282, 348
BsaJI CCNNGG 6 cut(s) 41, 336, 510, 630, 830, 836
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 42, 201, 260, 622, 831, 837
Bse118I RCCGGY 2 cut(s) 31, 284
Bse1I ACTGG 1 cut(s) 452
BseBI CCWGG 4 cut(s) 9, 202, 250, 838
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 6 cut(s) 41, 336, 510, 630, 830, 836
BseGI GGATG 3 cut(s) 141, 506, 804
BseLI CCNNNNNNNGG 7 cut(s) 14, 42, 201, 260, 622, 831, 837
BseMII CTCAG 1 cut(s) 595
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 3 cut(s) 77, 92, 329
BseSI GKGCMC 1 cut(s) 464
BseXI GCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 7 cut(s) 35, 120, 253, 759, 824, 835, 841
BshNI GGYRCC 4 cut(s) 266, 287, 347, 668
BshVI ATCGAT 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 685
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 7 cut(s) 14, 42, 201, 260, 622, 831, 837
BsmAI GTCTC 1 cut(s) 696
BsmFI GGGAC 1 cut(s) 647
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 7 cut(s) 35, 120, 253, 759, 824, 835, 841
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 3 cut(s) 464, 580, 685
Bsp143I GATC 4 cut(s) 11, 186, 318, 472
BspACI CCGC 5 cut(s) 101, 230, 241, 302, 482
BspANI GGCC 7 cut(s) 35, 120, 253, 759, 824, 835, 841
BspCNI CTCAG 1 cut(s) 594
BspDI ATCGAT 1 cut(s) 57
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 4 cut(s) 266, 287, 347, 668
BsrBI CCGCTC 1 cut(s) 241
BsrFI RCCGGY 2 cut(s) 31, 284
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 2 cut(s) 31, 284
BssECI CCNNGG 6 cut(s) 41, 336, 510, 630, 830, 836
BssMI GATC 4 cut(s) 11, 186, 318, 472
BssNI GRCGYC 3 cut(s) 87, 282, 348
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 4 cut(s) 9, 202, 250, 838
Bst4CI ACNGT 1 cut(s) 459
Bst6I CTCTTC 2 cut(s) 388, 566
BstACI GRCGYC 3 cut(s) 87, 282, 348
BstDEI CTNAG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 440
BstENI CCTNNNNNAGG 1 cut(s) 620
BstF5I GGATG 3 cut(s) 141, 506, 804
BstH2I RGCGCY 2 cut(s) 247, 351
BstHHI GCGC 2 cut(s) 246, 350
BstKTI GATC 4 cut(s) 14, 189, 321, 475
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 4 cut(s) 11, 186, 318, 472
BstMWI GCNNNNNNNGC 4 cut(s) 106, 250, 347, 356
BstNI CCWGG 4 cut(s) 9, 202, 250, 838
BstPI GGTNACC 1 cut(s) 440
BstSCI CCNGG 9 cut(s) 7, 41, 200, 248, 550, 629, 630, 830, 836
BstSLI GKGCMC 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 527
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 619
BsuRI GGCC 7 cut(s) 35, 120, 253, 759, 824, 835, 841
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 3 cut(s) 141, 506, 804
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 3 cut(s) 240, 445, 464
BveI ACCTGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 219
CfoI GCGC 2 cut(s) 246, 350
Cfr10I RCCGGY 2 cut(s) 31, 284
Cfr13I GGNCC 6 cut(s) 119, 204, 634, 822, 834, 840
Cfr9I CCCGGG 1 cut(s) 630
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 290
Csp6I GTAC 2 cut(s) 378, 537
CviAII CATG 4 cut(s) 220, 322, 647, 686
CviQI GTAC 2 cut(s) 378, 537
DdeI CTNAG 1 cut(s) 581
DinI GGCGCC 1 cut(s) 349
DpnI GATC 4 cut(s) 13, 188, 320, 474
DpnII GATC 4 cut(s) 11, 186, 318, 472
EaeI YGGCCR 3 cut(s) 33, 251, 757
Eam1104I CTCTTC 2 cut(s) 388, 566
EarI CTCTTC 2 cut(s) 388, 566
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 580
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 204, 634
Eco88I CYCGRG 1 cut(s) 630
Eco91I GGTNACC 1 cut(s) 440
EcoNI CCTNNNNNAGG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 634
EcoO65I GGTNACC 1 cut(s) 440
EcoRII CCWGG 4 cut(s) 7, 200, 248, 836
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 580
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 4 cut(s) 223, 325, 650, 689
FaqI GGGAC 1 cut(s) 647
FatI CATG 4 cut(s) 219, 321, 646, 685
FauI CCCGC 1 cut(s) 489
Fnu4HI GCNGC 4 cut(s) 101, 231, 242, 516
FokI GGATG 3 cut(s) 148, 493, 791
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 4 cut(s) 101, 231, 242, 516
FspBI CTAG 2 cut(s) 381, 525
GlaI GCGC 2 cut(s) 245, 349
GluI GCNGC 4 cut(s) 101, 231, 242, 516
HaeII RGCGCY 2 cut(s) 247, 351
HaeIII GGCC 7 cut(s) 35, 120, 253, 759, 824, 835, 841
HapII CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 246, 350
Hin1I GRCGYC 3 cut(s) 87, 282, 348
Hin1II CATG 4 cut(s) 223, 325, 650, 689
Hin6I GCGC 2 cut(s) 244, 348
HinP1I GCGC 2 cut(s) 244, 348
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HpaII CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
HphI GGTGA 7 cut(s) 29, 124, 157, 181, 206, 452, 619
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 5 cut(s) 177, 297, 493, 511, 622
Hpy188III TCNNGA 4 cut(s) 184, 220, 583, 695
Hpy8I GTNNAC 1 cut(s) 736
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 124, 136, 172, 557, 818
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4IV ACGT 2 cut(s) 87, 543
HpyCH4V TGCA 2 cut(s) 455, 646
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 250, 347, 356
HpyF3I CTNAG 1 cut(s) 581
HpySE526I ACGT 2 cut(s) 87, 543
Hsp92I GRCGYC 3 cut(s) 87, 282, 348
Hsp92II CATG 4 cut(s) 223, 325, 650, 689
HspAI GCGC 2 cut(s) 244, 348
KasI GGCGCC 1 cut(s) 347
KflI GGGWCCC 1 cut(s) 634
Kzo9I GATC 4 cut(s) 11, 186, 318, 472
LmnI GCTCC 2 cut(s) 238, 251
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 10
MaeI CTAG 2 cut(s) 381, 525
MaeII ACGT 2 cut(s) 87, 543
MaeIII GTNAC 1 cut(s) 440
MalI GATC 4 cut(s) 13, 188, 320, 474
MbiI CCGCTC 1 cut(s) 241
MboI GATC 4 cut(s) 11, 186, 318, 472
MboII GAAGA 4 cut(s) 290, 405, 506, 583
MfeI CAATTG 1 cut(s) 844
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 3 cut(s) 464, 580, 685
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 4 cut(s) 158, 257, 595, 844
MluNI TGGCCA 1 cut(s) 253
Mly113I GGCGCC 1 cut(s) 348
MmeI TCCRAC 1 cut(s) 63
Mox20I TGGCCA 1 cut(s) 253
MscI TGGCCA 1 cut(s) 253
Msp20I TGGCCA 1 cut(s) 253
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
MspR9I CCNGG 9 cut(s) 9, 43, 202, 250, 552, 631, 632, 832, 838
MunI CAATTG 1 cut(s) 844
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 4 cut(s) 9, 202, 250, 838
MwoI GCNNNNNNNGC 4 cut(s) 106, 250, 347, 356
NarI GGCGCC 1 cut(s) 348
NciI CCSGG 5 cut(s) 43, 552, 631, 632, 832
NdeII GATC 4 cut(s) 11, 186, 318, 472
NlaIII CATG 4 cut(s) 223, 325, 650, 689
NlaIV GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 1 cut(s) 440
PagI TCATGA 1 cut(s) 219
PaqCI CACCTGC 1 cut(s) 100
PctI GAATGC 1 cut(s) 335
PflMI CCANNNNNTGG 1 cut(s) 260
PkrI GCNGC 4 cut(s) 102, 232, 243, 517
PluTI GGCGCC 1 cut(s) 351
PpuMI RGGWCCY 1 cut(s) 634
Psp5II RGGWCCY 1 cut(s) 634
Psp6I CCWGG 4 cut(s) 7, 200, 248, 836
PspEI GGTNACC 1 cut(s) 440
PspGI CCWGG 4 cut(s) 7, 200, 248, 836
PspN4I GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
PspPI GGNCC 6 cut(s) 119, 204, 634, 822, 834, 840
PspPPI RGGWCCY 1 cut(s) 634
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 2 cut(s) 379, 538
RsaNI GTAC 2 cut(s) 378, 537
SatI GCNGC 4 cut(s) 101, 231, 242, 516
Sau3AI GATC 4 cut(s) 11, 186, 318, 472
Sau96I GGNCC 6 cut(s) 119, 204, 634, 822, 834, 840
ScaI AGTACT 1 cut(s) 379
ScrFI CCNGG 9 cut(s) 9, 43, 202, 250, 552, 631, 632, 832, 838
SduI GDGCHC 3 cut(s) 464, 580, 685
SfaNI GCATC 1 cut(s) 10
SfoI GGCGCC 1 cut(s) 349
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 2 cut(s) 204, 634
SmaI CCCGGG 1 cut(s) 632
SmlI CTYRAG 2 cut(s) 410, 695
SmoI CTYRAG 2 cut(s) 410, 695
SpeI ACTAGT 1 cut(s) 380
Sse9I AATT 4 cut(s) 158, 257, 595, 844
SsiI CCGC 5 cut(s) 101, 230, 241, 302, 482
SspDI GGCGCC 1 cut(s) 347
SspMI CTAG 2 cut(s) 381, 525
StyD4I CCNGG 9 cut(s) 7, 41, 200, 248, 550, 629, 630, 830, 836
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 459
TaiI ACGT 2 cut(s) 90, 546
TaqI TCGA 2 cut(s) 57, 327
TasI AATT 4 cut(s) 158, 257, 595, 844
TatI WGTACW 1 cut(s) 377
TauI GCSGC 3 cut(s) 103, 233, 244
TscAI CASTG 3 cut(s) 240, 452, 464
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 4 cut(s) 180, 291, 606, 663
TspGWI ACGGA 1 cut(s) 84
TspMI CCCGGG 1 cut(s) 630
TspRI CASTG 3 cut(s) 240, 452, 464
Van91I CCANNNNNTGG 1 cut(s) 260
VpaK11BI GGWCC 2 cut(s) 204, 634
XagI CCTNNNNNAGG 1 cut(s) 620
XapI RAATTY 3 cut(s) 158, 257, 595
XmaI CCCGGG 1 cut(s) 630
XspI CTAG 2 cut(s) 381, 525
ZraI GACGTC 1 cut(s) 88
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.