RLG00000016307

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016233
Physical Location & Seq
Forward (+)
5568823 .. 5569671
849 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016307

Sequence Viewer

Length: 849 bp
ATGAGACCAAGATCGGGTGATGCAACCCAACCGGCCAACCCCCGGTGGGCAGATATCGATGAGGAGGACGGACATGAGGAGTTGGACCTCCTCCTTTTGCCGCAAAAGCAGGTGATTGGCCCCGACGAGAAGGGGATAAAGAAGGTGATTGAATACAAATTCAATGAAAAGGGTCAGAAGGTCAAGATCACCACCACCACCAGGACCAAATCTCTCCTCATGAATAAGGCGGCCGTGGAGCGGCGCTCTTGGCCCAAATTCGGTGGTGCCGTCAATGAAGACGCCGGTGCCAATCTGACAGCGGTTTCCAAAGAGGAAGTCATGCTCGAACGCCCCAAGGCTCTTGGCGCCAACAAGGCACAAGAACTACCAAAGAGTACAAGTGGTAATGGAGGAGGCAATACTACTCTCAAGGTTTGTAGGACTTGTGGTAAGAAAGGTGACCACTGGACTGCACAGTGCCCATACAAAGATCTTGCCCCGCAGAGTTCCGAAGATATTAGCTCATCCGAGGCAGCCACAACTAGCAAGACTGGTACATATGTTCCTCCGGCTTTGAGAGAAGGAGGGAAGAGGGCTCTGAGGAATGATGAGAATTCAGTTCGGGTCAGCAACCTTTCGGAGGATACCCGGGACCCCGATTTGCACGAACTTTTCAGTCCTTTTGGTGCCATAAGCCGTGCTCATGTTGTTCTTGAGAGAGACACCCAAGTTAGCCGAGGATTTGGTTTTGTCAACTTTCTCAACAAGGAACACGGCCGAAGAGCCATAGACAAACTCAATGGGTATGGTTATGACAATCTCATCCTTCGGGTAGAATGGGCCACGCCCCGGCCCAGGCCCAGTTGA

Protein Analysis

283

Amino Acids

31.13

Weight (kDa)

8.99

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 37 - 159 2.9e-31 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 201 - 267 1.8e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 100
AccB1I GGYRCC 4 cut(s) 266, 287, 347, 668
AccBSI CCGCTC 1 cut(s) 241
AciI CCGC 5 cut(s) 101, 230, 241, 302, 482
AcoI YGGCCR 3 cut(s) 33, 231, 757
AcsI RAATTY 3 cut(s) 158, 257, 595
AcyI GRCGYC 2 cut(s) 282, 348
AfaI GTAC 2 cut(s) 379, 538
AfiI CCNNNNNNNGG 9 cut(s) 14, 42, 46, 201, 240, 260, 622, 831, 837
AgsI TTSAA 2 cut(s) 152, 163
AjnI CCWGG 2 cut(s) 200, 836
AluBI AGCT 1 cut(s) 504
AluI AGCT 1 cut(s) 504
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 1 cut(s) 696
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 8 cut(s) 33, 118, 231, 251, 757, 822, 833, 839
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 3 cut(s) 158, 257, 595
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspLEI GCGC 2 cut(s) 246, 350
AspS9I GGNCC 8 cut(s) 85, 119, 204, 252, 634, 822, 834, 840
AsuC2I CCSGG 4 cut(s) 43, 631, 632, 832
AsuHPI GGTGA 5 cut(s) 29, 124, 157, 181, 452
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 3 cut(s) 85, 204, 634
BaeGI GKGCMC 1 cut(s) 464
BaeI ACNNNNGTAYC 2 cut(s) 528, 561
BanI GGYRCC 4 cut(s) 266, 287, 347, 668
BanII GRGCYC 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 1 cut(s) 527
BceAI ACGGC 4 cut(s) 218, 254, 663, 772
BciT130I CCWGG 2 cut(s) 202, 838
BciVI GTATCC 1 cut(s) 619
BcnI CCSGG 4 cut(s) 43, 631, 632, 832
BcoDI GTCTC 1 cut(s) 696
BfaI CTAG 1 cut(s) 525
BfoI RGCGCY 2 cut(s) 247, 351
BfuAI ACCTGC 1 cut(s) 100
BfuI GTATCC 1 cut(s) 619
BglI GCCNNNNNGGC 1 cut(s) 356
BglII AGATCT 1 cut(s) 472
BisI GCNGC 4 cut(s) 101, 231, 242, 516
BlsI GCNGC 4 cut(s) 102, 232, 243, 517
Bme1390I CCNGG 6 cut(s) 43, 202, 631, 632, 832, 838
Bme18I GGWCC 3 cut(s) 85, 204, 634
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 8 cut(s) 85, 119, 204, 252, 634, 822, 834, 840
BmiI GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
BmrFI CCNGG 6 cut(s) 43, 202, 631, 632, 832, 838
BmrI ACTGGG 1 cut(s) 837
BmsI GCATC 1 cut(s) 10
BmuI ACTGGG 1 cut(s) 837
BpiI GAAGAC 1 cut(s) 285
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpuEI CTTGAG 2 cut(s) 395, 716
BpuMI CCSGG 4 cut(s) 43, 631, 632, 832
Bsa29I ATCGAT 1 cut(s) 57
BsaHI GRCGYC 2 cut(s) 282, 348
BsaJI CCNNGG 8 cut(s) 41, 234, 336, 510, 630, 718, 830, 836
Bsc4I CCNNNNNNNGG 9 cut(s) 14, 42, 46, 201, 240, 260, 622, 831, 837
Bse118I RCCGGY 2 cut(s) 31, 284
Bse1I ACTGG 3 cut(s) 452, 538, 843
BseBI CCWGG 2 cut(s) 202, 838
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 8 cut(s) 41, 234, 336, 510, 630, 718, 830, 836
BseGI GGATG 2 cut(s) 506, 804
BseLI CCNNNNNNNGG 9 cut(s) 14, 42, 46, 201, 240, 260, 622, 831, 837
BseMII CTCAG 1 cut(s) 572
BseNI ACTGG 3 cut(s) 452, 538, 843
BseRI GAGGAG 5 cut(s) 77, 80, 92, 206, 408
BseSI GKGCMC 1 cut(s) 464
BseX3I CGGCCG 2 cut(s) 231, 757
BseXI GCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 438
Bsh1285I CGRYCG 2 cut(s) 234, 760
BshFI GGCC 8 cut(s) 35, 120, 233, 253, 759, 824, 835, 841
BshNI GGYRCC 4 cut(s) 266, 287, 347, 668
BshVI ATCGAT 1 cut(s) 57
BsiEI CGRYCG 2 cut(s) 234, 760
BsiHKAI GWGCWC 1 cut(s) 685
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 9 cut(s) 14, 42, 46, 201, 240, 260, 622, 831, 837
BsmAI GTCTC 1 cut(s) 696
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 8 cut(s) 35, 120, 233, 253, 759, 824, 835, 841
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 3 cut(s) 464, 580, 685
Bsp143I GATC 3 cut(s) 11, 186, 472
BspACI CCGC 5 cut(s) 101, 230, 241, 302, 482
BspANI GGCC 8 cut(s) 35, 120, 233, 253, 759, 824, 835, 841
BspCNI CTCAG 1 cut(s) 573
BspDI ATCGAT 1 cut(s) 57
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
BspMI ACCTGC 1 cut(s) 100
BspQI GCTCTTC 1 cut(s) 757
BspT107I GGYRCC 4 cut(s) 266, 287, 347, 668
BsrBI CCGCTC 1 cut(s) 241
BsrFI RCCGGY 2 cut(s) 31, 284
BsrI ACTGG 3 cut(s) 452, 538, 843
BssAI RCCGGY 2 cut(s) 31, 284
BssECI CCNNGG 8 cut(s) 41, 234, 336, 510, 630, 718, 830, 836
BssMI GATC 3 cut(s) 11, 186, 472
BssNI GRCGYC 2 cut(s) 282, 348
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 2 cut(s) 202, 838
Bst4CI ACNGT 1 cut(s) 459
Bst6I CTCTTC 2 cut(s) 566, 757
BstACI GRCGYC 2 cut(s) 282, 348
BstDEI CTNAG 1 cut(s) 581
BstDSI CCRYGG 1 cut(s) 234
BstEII GGTNACC 1 cut(s) 440
BstENI CCTNNNNNAGG 1 cut(s) 620
BstF5I GGATG 2 cut(s) 506, 804
BstH2I RGCGCY 2 cut(s) 247, 351
BstHHI GCGC 2 cut(s) 246, 350
BstKTI GATC 3 cut(s) 14, 189, 475
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 3 cut(s) 11, 186, 472
BstMCI CGRYCG 2 cut(s) 234, 760
BstMWI GCNNNNNNNGC 4 cut(s) 106, 250, 347, 356
BstNI CCWGG 2 cut(s) 202, 838
BstPI GGTNACC 1 cut(s) 440
BstSCI CCNGG 6 cut(s) 41, 200, 629, 630, 830, 836
BstSLI GKGCMC 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 527
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BstZI CGGCCG 2 cut(s) 231, 757
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 619
BsuRI GGCC 8 cut(s) 35, 120, 233, 253, 759, 824, 835, 841
BsuTUI ATCGAT 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 506, 804
BtsIMutI CAGTG 2 cut(s) 445, 464
BveI ACCTGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 219
CfoI GCGC 2 cut(s) 246, 350
Cfr10I RCCGGY 2 cut(s) 31, 284
Cfr13I GGNCC 8 cut(s) 85, 119, 204, 252, 634, 822, 834, 840
Cfr9I CCCGGG 1 cut(s) 630
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 290
Csp6I GTAC 2 cut(s) 378, 537
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 4 cut(s) 74, 220, 322, 686
CviQI GTAC 2 cut(s) 378, 537
DdeI CTNAG 1 cut(s) 581
DinI GGCGCC 1 cut(s) 349
DpnI GATC 3 cut(s) 13, 188, 474
DpnII GATC 3 cut(s) 11, 186, 472
EaeI YGGCCR 3 cut(s) 33, 231, 757
EagI CGGCCG 2 cut(s) 231, 757
Eam1104I CTCTTC 2 cut(s) 566, 757
EarI CTCTTC 2 cut(s) 566, 757
EclXI CGGCCG 2 cut(s) 231, 757
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 580
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 3 cut(s) 85, 204, 634
Eco52I CGGCCG 2 cut(s) 231, 757
Eco88I CYCGRG 1 cut(s) 630
Eco91I GGTNACC 1 cut(s) 440
EcoNI CCTNNNNNAGG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 634
EcoO65I GGTNACC 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 2 cut(s) 200, 836
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 580
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 4 cut(s) 77, 223, 325, 689
FaqI GGGAC 1 cut(s) 647
FatI CATG 4 cut(s) 73, 219, 321, 685
FauI CCCGC 1 cut(s) 489
FauNDI CATATG 1 cut(s) 541
Fnu4HI GCNGC 4 cut(s) 101, 231, 242, 516
FokI GGATG 2 cut(s) 493, 791
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 4 cut(s) 101, 231, 242, 516
FspBI CTAG 1 cut(s) 525
GlaI GCGC 2 cut(s) 245, 349
GluI GCNGC 4 cut(s) 101, 231, 242, 516
HaeII RGCGCY 2 cut(s) 247, 351
HaeIII GGCC 8 cut(s) 35, 120, 233, 253, 759, 824, 835, 841
HapII CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 246, 350
Hin1I GRCGYC 2 cut(s) 282, 348
Hin1II CATG 4 cut(s) 77, 223, 325, 689
Hin6I GCGC 2 cut(s) 244, 348
HinP1I GCGC 2 cut(s) 244, 348
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HpaII CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
HphI GGTGA 5 cut(s) 29, 124, 157, 181, 452
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 6 cut(s) 177, 297, 493, 511, 582, 622
Hpy188III TCNNGA 3 cut(s) 184, 220, 695
Hpy8I GTNNAC 1 cut(s) 736
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 124, 136, 172, 557, 818
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 23, 455, 646
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 250, 347, 356
HpyF3I CTNAG 1 cut(s) 581
Hsp92I GRCGYC 2 cut(s) 282, 348
Hsp92II CATG 4 cut(s) 77, 223, 325, 689
HspAI GCGC 2 cut(s) 244, 348
KasI GGCGCC 1 cut(s) 347
KflI GGGWCCC 1 cut(s) 634
Kzo9I GATC 3 cut(s) 11, 186, 472
LguI GCTCTTC 1 cut(s) 757
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 10
MaeI CTAG 1 cut(s) 525
MaeIII GTNAC 1 cut(s) 440
MalI GATC 3 cut(s) 13, 188, 474
MbiI CCGCTC 1 cut(s) 241
MboI GATC 3 cut(s) 11, 186, 472
MboII GAAGA 4 cut(s) 290, 506, 583, 774
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 3 cut(s) 464, 580, 685
MluCI AATT 3 cut(s) 158, 257, 595
Mly113I GGCGCC 1 cut(s) 348
MmeI TCCRAC 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 6 cut(s) 32, 43, 285, 551, 631, 832
MspR9I CCNGG 6 cut(s) 43, 202, 631, 632, 832, 838
MvaI CCWGG 2 cut(s) 202, 838
MwoI GCNNNNNNNGC 4 cut(s) 106, 250, 347, 356
NarI GGCGCC 1 cut(s) 348
NciI CCSGG 4 cut(s) 43, 631, 632, 832
NdeI CATATG 1 cut(s) 541
NdeII GATC 3 cut(s) 11, 186, 472
NlaIII CATG 4 cut(s) 77, 223, 325, 689
NlaIV GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
NmeAIII GCCGAG 1 cut(s) 743
NmuCI GTSAC 1 cut(s) 440
PagI TCATGA 1 cut(s) 219
PaqCI CACCTGC 1 cut(s) 100
PciSI GCTCTTC 1 cut(s) 757
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PkrI GCNGC 4 cut(s) 102, 232, 243, 517
PluTI GGCGCC 1 cut(s) 351
PpuMI RGGWCCY 1 cut(s) 634
Psp5II RGGWCCY 1 cut(s) 634
Psp6I CCWGG 2 cut(s) 200, 836
PspEI GGTNACC 1 cut(s) 440
PspGI CCWGG 2 cut(s) 200, 836
PspN4I GGNNCC 7 cut(s) 121, 268, 289, 349, 635, 636, 670
PspPI GGNCC 8 cut(s) 85, 119, 204, 252, 634, 822, 834, 840
PspPPI RGGWCCY 1 cut(s) 634
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 2 cut(s) 379, 538
RsaNI GTAC 2 cut(s) 378, 537
SapI GCTCTTC 1 cut(s) 757
SatI GCNGC 4 cut(s) 101, 231, 242, 516
Sau3AI GATC 3 cut(s) 11, 186, 472
Sau96I GGNCC 8 cut(s) 85, 119, 204, 252, 634, 822, 834, 840
ScrFI CCNGG 6 cut(s) 43, 202, 631, 632, 832, 838
SduI GDGCHC 3 cut(s) 464, 580, 685
SetI ASST 8 cut(s) 90, 114, 147, 183, 417, 442, 506, 618
SfaNI GCATC 1 cut(s) 10
SfoI GGCGCC 1 cut(s) 349
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 3 cut(s) 85, 204, 634
SmaI CCCGGG 1 cut(s) 632
SmlI CTYRAG 2 cut(s) 410, 695
SmoI CTYRAG 2 cut(s) 410, 695
Sse9I AATT 3 cut(s) 158, 257, 595
SsiI CCGC 5 cut(s) 101, 230, 241, 302, 482
SspDI GGCGCC 1 cut(s) 347
SspMI CTAG 1 cut(s) 525
StyD4I CCNGG 6 cut(s) 41, 200, 629, 630, 830, 836
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 57, 327
TasI AATT 3 cut(s) 158, 257, 595
TatI WGTACW 1 cut(s) 377
TauI GCSGC 3 cut(s) 103, 233, 244
TscAI CASTG 2 cut(s) 452, 464
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 3 cut(s) 180, 236, 291
TspGWI ACGGA 1 cut(s) 84
TspMI CCCGGG 1 cut(s) 630
TspRI CASTG 2 cut(s) 452, 464
VpaK11BI GGWCC 3 cut(s) 85, 204, 634
XagI CCTNNNNNAGG 1 cut(s) 620
XapI RAATTY 3 cut(s) 158, 257, 595
XmaI CCCGGG 1 cut(s) 630
XspI CTAG 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.