Rh2CG073200

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016233
Physical Location & Seq
Forward (+)
5986991 .. 5988168
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG073200.1

Sequence Viewer

Length: 855 bp
ATGAGACCAGGATCGGGTGATGCTACCCAACCGGCCAAGCCCCGGTGGGCAGATATCGATGAGGAGGACGGACAGGAGGAGTTGGACCTCCTCCTTTTGCCGCCAAAGCAGGTGATTGGCCCCAACGAGAAGGGGATGGAGAAGGTGATTGAATACAAATTCAATGAAAAGGGTCGGAAGGTCAAGATCACCACCACCACCACCACCAGGACCAAATCTCACCTCATGAGTAAGGCGGCCGTGGAGCGGCGCTCCTGGCCAAAATTTGGTGGTGCCGTCAATGAAGACGCCGGTGCCAATCTGACAGCGGTTTCCAAAGAGGAGATCATGCTCGAACGCCCCAAGGCTCTTGGCGCCAACAAGGCACAAGAACTACCAAAGAGTACTAGTGGTAAAGGAGGAGGCAATACTACTCTCAAGGTTTGTAGGACTTGTGGTAAGAAAGGTGATCACTGGACTGCACAGTGCCCATACAAAGATCTTGCCTCGCCGAGTTCCGAAGATATTAGCTCATCCGAGGCAGCCACAACTAGCAAGATTGGTACATACGTTCCTCCGGGTTTGAGAGAAGGAGTGAAGAGGGCTCTGAGGAATGATGAAAATTCAATTCGGGTGAGCAACCTTTCGGAGGATACCCGGGACCCCGATTTGCATGAACTTTTCAGTCCTTTTGGTGCCATAAGCCGTGCTCATGTTGTTCTTGAGAGAGACACCCAAGTTAGCAGAGGATTTGGTTTTGTTGACTTTCTCAACAAGGAACACGGCCAAAGAGCCATAGACAAACTCAATGGGTATGGTTATGACAATCTCATCCTTCGGGTAGAATGGGCCACGCCCCGGCCCAGGCCCAATTGA

Protein Analysis

284

Amino Acids

31.29

Weight (kDa)

8.82

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 37 - 161 1.3e-30 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 203 - 269 1.3e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 100
AccB1I GGYRCC 4 cut(s) 272, 293, 353, 674
AccB7I CCANNNNNTGG 1 cut(s) 266
AccBSI CCGCTC 1 cut(s) 247
AciI CCGC 4 cut(s) 101, 236, 247, 308
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 4 cut(s) 33, 237, 257, 763
AcsI RAATTY 3 cut(s) 158, 263, 601
AcyI GRCGYC 2 cut(s) 288, 354
AfaI GTAC 2 cut(s) 385, 544
AfiI CCNNNNNNNGG 8 cut(s) 14, 42, 207, 246, 266, 628, 837, 843
AgsI TTSAA 3 cut(s) 152, 163, 606
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 4 cut(s) 7, 206, 254, 842
AluBI AGCT 1 cut(s) 510
AluI AGCT 1 cut(s) 510
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 1 cut(s) 702
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 8 cut(s) 33, 118, 237, 257, 763, 828, 839, 845
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 3 cut(s) 158, 263, 601
AspLEI GCGC 2 cut(s) 252, 356
AspS9I GGNCC 7 cut(s) 85, 119, 210, 640, 828, 840, 846
AsuC2I CCSGG 5 cut(s) 43, 558, 637, 638, 838
AsuHPI GGTGA 7 cut(s) 29, 124, 157, 181, 212, 458, 625
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 3 cut(s) 85, 210, 640
BaeGI GKGCMC 1 cut(s) 470
BaeI ACNNNNGTAYC 2 cut(s) 534, 567
BalI TGGCCA 1 cut(s) 259
BanI GGYRCC 4 cut(s) 272, 293, 353, 674
BanII GRGCYC 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 1 cut(s) 533
BccI CCATC 1 cut(s) 130
BceAI ACGGC 4 cut(s) 224, 260, 669, 778
BciT130I CCWGG 4 cut(s) 9, 208, 256, 844
BciVI GTATCC 1 cut(s) 625
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 5 cut(s) 43, 558, 637, 638, 838
BcoDI GTCTC 1 cut(s) 702
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 2 cut(s) 387, 531
BfoI RGCGCY 2 cut(s) 253, 357
BfuAI ACCTGC 1 cut(s) 100
BfuI GTATCC 1 cut(s) 625
BglI GCCNNNNNGGC 1 cut(s) 362
BglII AGATCT 1 cut(s) 478
BisI GCNGC 4 cut(s) 101, 237, 248, 522
BlsI GCNGC 4 cut(s) 102, 238, 249, 523
BmcAI AGTACT 1 cut(s) 385
Bme1390I CCNGG 9 cut(s) 9, 43, 208, 256, 558, 637, 638, 838, 844
Bme18I GGWCC 3 cut(s) 85, 210, 640
BmeT110I CYCGRG 1 cut(s) 636
BmgT120I GGNCC 7 cut(s) 85, 119, 210, 640, 828, 840, 846
BmiI GGNNCC 7 cut(s) 121, 274, 295, 355, 641, 642, 676
BmrFI CCNGG 9 cut(s) 9, 43, 208, 256, 558, 637, 638, 838, 844
BmsI GCATC 1 cut(s) 10
BpiI GAAGAC 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpuEI CTTGAG 2 cut(s) 401, 722
BpuMI CCSGG 5 cut(s) 43, 558, 637, 638, 838
Bsa29I ATCGAT 1 cut(s) 57
BsaHI GRCGYC 2 cut(s) 288, 354
BsaJI CCNNGG 7 cut(s) 41, 240, 342, 516, 636, 836, 842
Bsc4I CCNNNNNNNGG 8 cut(s) 14, 42, 207, 246, 266, 628, 837, 843
Bse118I RCCGGY 2 cut(s) 31, 290
Bse1I ACTGG 1 cut(s) 458
BseBI CCWGG 4 cut(s) 9, 208, 256, 844
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 7 cut(s) 41, 240, 342, 516, 636, 836, 842
BseGI GGATG 3 cut(s) 141, 512, 810
BseLI CCNNNNNNNGG 8 cut(s) 14, 42, 207, 246, 266, 628, 837, 843
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 1 cut(s) 458
BseRI GAGGAG 5 cut(s) 77, 80, 92, 335, 414
BseSI GKGCMC 1 cut(s) 470
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 533
BsgI GTGCAG 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 8 cut(s) 35, 120, 239, 259, 765, 830, 841, 847
BshNI GGYRCC 4 cut(s) 272, 293, 353, 674
BshVI ATCGAT 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 6 cut(s) 32, 43, 291, 557, 637, 838
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 8 cut(s) 14, 42, 207, 246, 266, 628, 837, 843
BsmAI GTCTC 1 cut(s) 702
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 8 cut(s) 35, 120, 239, 259, 765, 830, 841, 847
BsoBI CYCGRG 1 cut(s) 636
Bsp1286I GDGCHC 3 cut(s) 470, 586, 691
Bsp143I GATC 5 cut(s) 11, 186, 324, 448, 478
BspACI CCGC 4 cut(s) 101, 236, 247, 308
BspANI GGCC 8 cut(s) 35, 120, 239, 259, 765, 830, 841, 847
BspCNI CTCAG 1 cut(s) 579
BspDI ATCGAT 1 cut(s) 57
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 7 cut(s) 121, 274, 295, 355, 641, 642, 676
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 4 cut(s) 272, 293, 353, 674
BsrBI CCGCTC 1 cut(s) 247
BsrFI RCCGGY 2 cut(s) 31, 290
BsrI ACTGG 1 cut(s) 458
BssAI RCCGGY 2 cut(s) 31, 290
BssECI CCNNGG 7 cut(s) 41, 240, 342, 516, 636, 836, 842
BssMI GATC 5 cut(s) 11, 186, 324, 448, 478
BssNI GRCGYC 2 cut(s) 288, 354
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 4 cut(s) 9, 208, 256, 844
Bst4CI ACNGT 1 cut(s) 465
Bst6I CTCTTC 1 cut(s) 572
BstACI GRCGYC 2 cut(s) 288, 354
BstDEI CTNAG 1 cut(s) 587
BstDSI CCRYGG 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 3 cut(s) 141, 512, 810
BstH2I RGCGCY 2 cut(s) 253, 357
BstHHI GCGC 2 cut(s) 252, 356
BstKTI GATC 5 cut(s) 14, 189, 327, 451, 481
BstMAI GTCTC 1 cut(s) 702
BstMBI GATC 5 cut(s) 11, 186, 324, 448, 478
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 4 cut(s) 106, 256, 353, 362
BstNI CCWGG 4 cut(s) 9, 208, 256, 844
BstSCI CCNGG 9 cut(s) 7, 41, 206, 254, 556, 635, 636, 836, 842
BstSLI GKGCMC 1 cut(s) 470
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 291
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BstZI CGGCCG 1 cut(s) 237
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 625
BsuRI GGCC 8 cut(s) 35, 120, 239, 259, 765, 830, 841, 847
BsuTUI ATCGAT 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 3 cut(s) 141, 512, 810
BtsIMutI CAGTG 2 cut(s) 451, 470
BveI ACCTGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 225
CfoI GCGC 2 cut(s) 252, 356
Cfr10I RCCGGY 2 cut(s) 31, 290
Cfr13I GGNCC 7 cut(s) 85, 119, 210, 640, 828, 840, 846
Cfr9I CCCGGG 1 cut(s) 636
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 384, 543
CviAII CATG 4 cut(s) 226, 328, 653, 692
CviQI GTAC 2 cut(s) 384, 543
DdeI CTNAG 1 cut(s) 587
DinI GGCGCC 1 cut(s) 355
DpnI GATC 5 cut(s) 13, 188, 326, 450, 480
DpnII GATC 5 cut(s) 11, 186, 324, 448, 478
EaeI YGGCCR 4 cut(s) 33, 237, 257, 763
EagI CGGCCG 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
EclXI CGGCCG 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 586
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 3 cut(s) 85, 210, 640
Eco52I CGGCCG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 636
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 640
EcoRII CCWGG 4 cut(s) 7, 206, 254, 842
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 586
EgeI GGCGCC 1 cut(s) 355
EheI GGCGCC 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 4 cut(s) 229, 331, 656, 695
FaqI GGGAC 1 cut(s) 653
FatI CATG 4 cut(s) 225, 327, 652, 691
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 4 cut(s) 101, 237, 248, 522
FokI GGATG 3 cut(s) 148, 499, 797
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 4 cut(s) 101, 237, 248, 522
FspBI CTAG 2 cut(s) 387, 531
GlaI GCGC 2 cut(s) 251, 355
GluI GCNGC 4 cut(s) 101, 237, 248, 522
HaeII RGCGCY 2 cut(s) 253, 357
HaeIII GGCC 8 cut(s) 35, 120, 239, 259, 765, 830, 841, 847
HapII CCGG 6 cut(s) 32, 43, 291, 557, 637, 838
HgaI GACGC 1 cut(s) 296
HhaI GCGC 2 cut(s) 252, 356
Hin1I GRCGYC 2 cut(s) 288, 354
Hin1II CATG 4 cut(s) 229, 331, 656, 695
Hin6I GCGC 2 cut(s) 250, 354
HinP1I GCGC 2 cut(s) 250, 354
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HpaII CCGG 6 cut(s) 32, 43, 291, 557, 637, 838
HphI GGTGA 7 cut(s) 29, 124, 157, 181, 212, 458, 625
Hpy166II GTNNAC 1 cut(s) 742
Hpy188I TCNGA 6 cut(s) 177, 303, 499, 517, 588, 628
Hpy188III TCNNGA 3 cut(s) 184, 226, 701
Hpy8I GTNNAC 1 cut(s) 742
HpyAV CCTTC 5 cut(s) 124, 136, 172, 563, 824
HpyCH4III ACNGT 1 cut(s) 465
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 2 cut(s) 461, 652
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 256, 353, 362
HpyF3I CTNAG 1 cut(s) 587
HpySE526I ACGT 1 cut(s) 549
Hsp92I GRCGYC 2 cut(s) 288, 354
Hsp92II CATG 4 cut(s) 229, 331, 656, 695
HspAI GCGC 2 cut(s) 250, 354
KasI GGCGCC 1 cut(s) 353
KflI GGGWCCC 1 cut(s) 640
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 5 cut(s) 11, 186, 324, 448, 478
LmnI GCTCC 2 cut(s) 244, 257
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 1 cut(s) 10
MaeI CTAG 2 cut(s) 387, 531
MaeII ACGT 1 cut(s) 549
MalI GATC 5 cut(s) 13, 188, 326, 450, 480
MbiI CCGCTC 1 cut(s) 247
MboI GATC 5 cut(s) 11, 186, 324, 448, 478
MboII GAAGA 3 cut(s) 296, 512, 589
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 3 cut(s) 470, 586, 691
MlsI TGGCCA 1 cut(s) 259
MluCI AATT 5 cut(s) 158, 263, 601, 606, 850
MluNI TGGCCA 1 cut(s) 259
Mly113I GGCGCC 1 cut(s) 354
MmeI TCCRAC 2 cut(s) 63, 155
Mox20I TGGCCA 1 cut(s) 259
MscI TGGCCA 1 cut(s) 259
Msp20I TGGCCA 1 cut(s) 259
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 6 cut(s) 32, 43, 291, 557, 637, 838
MspR9I CCNGG 9 cut(s) 9, 43, 208, 256, 558, 637, 638, 838, 844
MunI CAATTG 1 cut(s) 850
MvaI CCWGG 4 cut(s) 9, 208, 256, 844
MwoI GCNNNNNNNGC 4 cut(s) 106, 256, 353, 362
NarI GGCGCC 1 cut(s) 354
NciI CCSGG 5 cut(s) 43, 558, 637, 638, 838
NdeII GATC 5 cut(s) 11, 186, 324, 448, 478
NlaIII CATG 4 cut(s) 229, 331, 656, 695
NlaIV GGNNCC 7 cut(s) 121, 274, 295, 355, 641, 642, 676
NmeAIII GCCGAG 1 cut(s) 516
PagI TCATGA 1 cut(s) 225
PaqCI CACCTGC 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 4 cut(s) 102, 238, 249, 523
PluTI GGCGCC 1 cut(s) 357
PpuMI RGGWCCY 1 cut(s) 640
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 4 cut(s) 7, 206, 254, 842
PspGI CCWGG 4 cut(s) 7, 206, 254, 842
PspN4I GGNNCC 7 cut(s) 121, 274, 295, 355, 641, 642, 676
PspPI GGNCC 7 cut(s) 85, 119, 210, 640, 828, 840, 846
PspPPI RGGWCCY 1 cut(s) 640
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 2 cut(s) 385, 544
RsaNI GTAC 2 cut(s) 384, 543
SatI GCNGC 4 cut(s) 101, 237, 248, 522
Sau3AI GATC 5 cut(s) 11, 186, 324, 448, 478
Sau96I GGNCC 7 cut(s) 85, 119, 210, 640, 828, 840, 846
ScaI AGTACT 1 cut(s) 385
ScrFI CCNGG 9 cut(s) 9, 43, 208, 256, 558, 637, 638, 838, 844
SduI GDGCHC 3 cut(s) 470, 586, 691
SfaNI GCATC 1 cut(s) 10
SfoI GGCGCC 1 cut(s) 355
SgrAI CRCCGGYG 1 cut(s) 290
SinI GGWCC 3 cut(s) 85, 210, 640
SmaI CCCGGG 1 cut(s) 638
SmlI CTYRAG 2 cut(s) 416, 701
SmoI CTYRAG 2 cut(s) 416, 701
SpeI ACTAGT 1 cut(s) 386
Sse9I AATT 5 cut(s) 158, 263, 601, 606, 850
SsiI CCGC 4 cut(s) 101, 236, 247, 308
SspDI GGCGCC 1 cut(s) 353
SspMI CTAG 2 cut(s) 387, 531
StyD4I CCNGG 9 cut(s) 7, 41, 206, 254, 556, 635, 636, 836, 842
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 465
TaiI ACGT 1 cut(s) 552
TaqI TCGA 2 cut(s) 57, 333
TasI AATT 5 cut(s) 158, 263, 601, 606, 850
TatI WGTACW 1 cut(s) 383
TauI GCSGC 3 cut(s) 103, 239, 250
TscAI CASTG 2 cut(s) 458, 470
TseI GCWGC 1 cut(s) 521
TspDTI ATGAA 4 cut(s) 180, 297, 612, 669
TspGWI ACGGA 1 cut(s) 84
TspMI CCCGGG 1 cut(s) 636
TspRI CASTG 2 cut(s) 458, 470
Van91I CCANNNNNTGG 1 cut(s) 266
VpaK11BI GGWCC 3 cut(s) 85, 210, 640
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 3 cut(s) 158, 263, 601
XmaI CCCGGG 1 cut(s) 636
XspI CTAG 2 cut(s) 387, 531
ZrmI AGTACT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.