Rw2G006080

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016233
Physical Location & Seq
Reverse (-)
5809116 .. 5811886
2771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006080.1

Sequence Viewer

Length: 825 bp
ATGAGACCAGGATCGGGTAATGCTACCCAACCGGCCAAGCCCCGGTGGGCAGATATCGATGAGGAGGACGGACAGGAGGAGTTGGATGTCCTCCTTTTGCAGCCAAAGCAGGTGATTGGCCCCGAAGAGAAGGGGATGGAGAAGGTGATTGAATACAAATTCAATGAAAAGGGTCAGAAGGTCAAGATCACCACCACCACCTGGACCAAATCTCACCTCATGAGTAAGGCGGCAGTGGAGCGCTGCATCAAGCGGCGCTCCTGGCCAAAATTTGGTGGTGCCGTCAAAGAAGACGCCGGTGCCAATCTGACAGCGGTTTCCAAAGAGGAGATCATGCTCGAATGCCCCAAGAGTACTAGTGGTAATGGAGGAGGCAATACTACTCTCAAGGTTTGTAGGACTTGTGGTAAGAAAGGTGACCACTGGATTGCACAGTGCCCATACAAAGATCTTGTCCCGCCGAGTTCCAAAGATATTAGCTCATCCGAGGCAGCCACAACTAGTAAGATTGGTACATACGTTCCTCCGGGTTTGAGAGAAGGAGTGAAGAGGGCTCTGAGGAATGATGAAAATTCAGTTCGGGTGAGCAACCTTTTGGAGGATACCCGGGACCCCGATTTGCATGAACTTTTCAGTCCTTTTGGTGCCATAAGCTGTGCTCATGCTGTTCTTGAGAGAGACACCCAAGTTAGCAGAGGATTTGGTTTTGTTGAATTTCTCAACAAGGAACACGGCCAAAGAGCCATAGACAAACTCAATGGGTATGGTTATGACAATCTCATCCTTCGGGTAGAATGGGCCACGCCCCGGCCCAGGCCCAATTGA

Protein Analysis

274

Amino Acids

30.34

Weight (kDa)

8.43

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 37 - 151 3.1e-21 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 193 - 259 2.1e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 100
AccB1I GGYRCC 3 cut(s) 278, 299, 644
AccB7I CCANNNNNTGG 2 cut(s) 201, 272
AciI CCGC 4 cut(s) 230, 253, 314, 458
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 3 cut(s) 33, 263, 733
AcsI RAATTY 4 cut(s) 158, 269, 571, 713
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 2 cut(s) 355, 514
AfeI AGCGCT 1 cut(s) 242
AfiI CCNNNNNNNGG 7 cut(s) 14, 42, 201, 272, 598, 807, 813
AgsI TTSAA 3 cut(s) 152, 163, 713
AhlI ACTAGT 2 cut(s) 356, 500
AjnI CCWGG 4 cut(s) 7, 200, 260, 812
AluBI AGCT 2 cut(s) 480, 654
AluI AGCT 2 cut(s) 480, 654
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 1 cut(s) 672
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 606
Aor51HI AGCGCT 1 cut(s) 242
AoxI GGCC 7 cut(s) 33, 118, 263, 733, 798, 809, 815
ApeKI GCWGC 3 cut(s) 100, 243, 491
ApoI RAATTY 4 cut(s) 158, 269, 571, 713
ArsI GACNNNNNNTTYG 2 cut(s) 438, 470
AspLEI GCGC 2 cut(s) 243, 258
AspS9I GGNCC 6 cut(s) 119, 204, 610, 798, 810, 816
AsuC2I CCSGG 5 cut(s) 43, 528, 607, 608, 808
AsuHPI GGTGA 6 cut(s) 124, 157, 181, 206, 428, 595
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 2 cut(s) 204, 610
BaeGI GKGCMC 1 cut(s) 440
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BalI TGGCCA 1 cut(s) 265
BanI GGYRCC 3 cut(s) 278, 299, 644
BanII GRGCYC 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 661
BbvI GCAGC 3 cut(s) 112, 230, 503
BccI CCATC 1 cut(s) 130
BceAI ACGGC 2 cut(s) 266, 748
BciT130I CCWGG 4 cut(s) 9, 202, 262, 814
BciVI GTATCC 1 cut(s) 595
BcnI CCSGG 5 cut(s) 43, 528, 607, 608, 808
BcoDI GTCTC 1 cut(s) 672
BcuI ACTAGT 2 cut(s) 356, 500
BfaI CTAG 2 cut(s) 357, 501
BfoI RGCGCY 2 cut(s) 244, 259
BfuAI ACCTGC 1 cut(s) 100
BfuI GTATCC 1 cut(s) 595
BglII AGATCT 1 cut(s) 448
BisI GCNGC 5 cut(s) 101, 231, 244, 254, 492
BlsI GCNGC 5 cut(s) 102, 232, 245, 255, 493
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 9 cut(s) 9, 43, 202, 262, 528, 607, 608, 808, 814
Bme18I GGWCC 2 cut(s) 204, 610
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 6 cut(s) 119, 204, 610, 798, 810, 816
BmiI GGNNCC 6 cut(s) 121, 280, 301, 611, 612, 646
BmrFI CCNGG 9 cut(s) 9, 43, 202, 262, 528, 607, 608, 808, 814
BmsI GCATC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 297
BpuEI CTTGAG 2 cut(s) 371, 692
BpuMI CCSGG 5 cut(s) 43, 528, 607, 608, 808
Bsa29I ATCGAT 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 5 cut(s) 41, 486, 606, 806, 812
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 42, 201, 272, 598, 807, 813
Bse118I RCCGGY 2 cut(s) 31, 296
Bse1I ACTGG 1 cut(s) 428
BseBI CCWGG 4 cut(s) 9, 202, 262, 814
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 5 cut(s) 41, 486, 606, 806, 812
BseGI GGATG 4 cut(s) 91, 141, 482, 780
BseLI CCNNNNNNNGG 7 cut(s) 14, 42, 201, 272, 598, 807, 813
BseMII CTCAG 1 cut(s) 548
BseNI ACTGG 1 cut(s) 428
BseRI GAGGAG 4 cut(s) 77, 92, 341, 384
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 3 cut(s) 112, 230, 503
BshFI GGCC 7 cut(s) 35, 120, 265, 735, 800, 811, 817
BshNI GGYRCC 3 cut(s) 278, 299, 644
BshVI ATCGAT 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 661
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 6 cut(s) 32, 43, 297, 527, 607, 808
BslFI GGGAC 2 cut(s) 440, 623
BslI CCNNNNNNNGG 7 cut(s) 14, 42, 201, 272, 598, 807, 813
BsmAI GTCTC 1 cut(s) 672
BsmFI GGGAC 2 cut(s) 440, 623
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 7 cut(s) 35, 120, 265, 735, 800, 811, 817
BsoBI CYCGRG 1 cut(s) 606
Bsp1286I GDGCHC 3 cut(s) 440, 556, 661
Bsp143I GATC 4 cut(s) 11, 186, 330, 448
BspACI CCGC 4 cut(s) 230, 253, 314, 458
BspANI GGCC 7 cut(s) 35, 120, 265, 735, 800, 811, 817
BspCNI CTCAG 1 cut(s) 549
BspDI ATCGAT 1 cut(s) 57
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 6 cut(s) 121, 280, 301, 611, 612, 646
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 3 cut(s) 278, 299, 644
BsrFI RCCGGY 2 cut(s) 31, 296
BsrI ACTGG 1 cut(s) 428
BssAI RCCGGY 2 cut(s) 31, 296
BssECI CCNNGG 5 cut(s) 41, 486, 606, 806, 812
BssMI GATC 4 cut(s) 11, 186, 330, 448
BssNI GRCGYC 1 cut(s) 294
Bst2UI CCWGG 4 cut(s) 9, 202, 262, 814
Bst4CI ACNGT 1 cut(s) 435
Bst6I CTCTTC 2 cut(s) 120, 542
BstACI GRCGYC 1 cut(s) 294
BstDEI CTNAG 1 cut(s) 557
BstEII GGTNACC 1 cut(s) 416
BstENI CCTNNNNNAGG 1 cut(s) 596
BstF5I GGATG 4 cut(s) 91, 141, 482, 780
BstH2I RGCGCY 2 cut(s) 244, 259
BstHHI GCGC 2 cut(s) 243, 258
BstKTI GATC 4 cut(s) 14, 189, 333, 451
BstMAI GTCTC 1 cut(s) 672
BstMBI GATC 4 cut(s) 11, 186, 330, 448
BstMWI GCNNNNNNNGC 2 cut(s) 106, 262
BstNI CCWGG 4 cut(s) 9, 202, 262, 814
BstPI GGTNACC 1 cut(s) 416
BstSCI CCNGG 9 cut(s) 7, 41, 200, 260, 526, 605, 606, 806, 812
BstSLI GKGCMC 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 112, 230, 503
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 7 cut(s) 35, 120, 265, 735, 800, 811, 817
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 4 cut(s) 91, 141, 482, 780
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 3 cut(s) 240, 421, 440
BveI ACCTGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 219
CfoI GCGC 2 cut(s) 243, 258
Cfr10I RCCGGY 2 cut(s) 31, 296
Cfr13I GGNCC 6 cut(s) 119, 204, 610, 798, 810, 816
Cfr9I CCCGGG 1 cut(s) 606
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 354, 513
CviAII CATG 4 cut(s) 220, 334, 623, 662
CviQI GTAC 2 cut(s) 354, 513
DdeI CTNAG 1 cut(s) 557
DpnI GATC 4 cut(s) 13, 188, 332, 450
DpnII GATC 4 cut(s) 11, 186, 330, 448
EaeI YGGCCR 3 cut(s) 33, 263, 733
Eam1104I CTCTTC 2 cut(s) 120, 542
EarI CTCTTC 2 cut(s) 120, 542
Eco24I GRGCYC 1 cut(s) 556
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 204, 610
Eco47III AGCGCT 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 606
Eco91I GGTNACC 1 cut(s) 416
EcoNI CCTNNNNNAGG 1 cut(s) 596
EcoO109I RGGNCCY 1 cut(s) 610
EcoO65I GGTNACC 1 cut(s) 416
EcoRII CCWGG 4 cut(s) 7, 200, 260, 812
EcoRV GATATC 1 cut(s) 55
EcoT38I GRGCYC 1 cut(s) 556
FaeI CATG 4 cut(s) 223, 337, 626, 665
FaqI GGGAC 2 cut(s) 440, 623
FatI CATG 4 cut(s) 219, 333, 622, 661
FauI CCCGC 1 cut(s) 465
Fnu4HI GCNGC 5 cut(s) 101, 231, 244, 254, 492
FokI GGATG 4 cut(s) 98, 148, 469, 767
FriOI GRGCYC 1 cut(s) 556
Fsp4HI GCNGC 5 cut(s) 101, 231, 244, 254, 492
FspBI CTAG 2 cut(s) 357, 501
GlaI GCGC 2 cut(s) 242, 257
GluI GCNGC 5 cut(s) 101, 231, 244, 254, 492
HaeII RGCGCY 2 cut(s) 244, 259
HaeIII GGCC 7 cut(s) 35, 120, 265, 735, 800, 811, 817
HapII CCGG 6 cut(s) 32, 43, 297, 527, 607, 808
HgaI GACGC 1 cut(s) 302
HhaI GCGC 2 cut(s) 243, 258
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 4 cut(s) 223, 337, 626, 665
Hin6I GCGC 2 cut(s) 241, 256
HinP1I GCGC 2 cut(s) 241, 256
HpaII CCGG 6 cut(s) 32, 43, 297, 527, 607, 808
HphI GGTGA 6 cut(s) 124, 157, 181, 206, 428, 595
Hpy188I TCNGA 4 cut(s) 177, 309, 487, 558
Hpy188III TCNNGA 3 cut(s) 184, 220, 671
HpyAV CCTTC 5 cut(s) 124, 136, 172, 533, 794
HpyCH4III ACNGT 1 cut(s) 435
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 4 cut(s) 100, 246, 431, 622
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 262
HpyF3I CTNAG 1 cut(s) 557
HpySE526I ACGT 1 cut(s) 519
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 4 cut(s) 223, 337, 626, 665
HspAI GCGC 2 cut(s) 241, 256
KflI GGGWCCC 1 cut(s) 610
Kzo9I GATC 4 cut(s) 11, 186, 330, 448
LmnI GCTCC 2 cut(s) 238, 263
Lsp1109I GCAGC 3 cut(s) 112, 230, 503
LweI GCATC 1 cut(s) 255
MaeI CTAG 2 cut(s) 357, 501
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 1 cut(s) 416
MalI GATC 4 cut(s) 13, 188, 332, 450
MboI GATC 4 cut(s) 11, 186, 330, 448
MboII GAAGA 3 cut(s) 137, 302, 559
MfeI CAATTG 1 cut(s) 820
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 3 cut(s) 440, 556, 661
MlsI TGGCCA 1 cut(s) 265
MluCI AATT 5 cut(s) 158, 269, 571, 713, 820
MluNI TGGCCA 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 63
Mox20I TGGCCA 1 cut(s) 265
MscI TGGCCA 1 cut(s) 265
Msp20I TGGCCA 1 cut(s) 265
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 6 cut(s) 32, 43, 297, 527, 607, 808
MspR9I CCNGG 9 cut(s) 9, 43, 202, 262, 528, 607, 608, 808, 814
MunI CAATTG 1 cut(s) 820
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 4 cut(s) 9, 202, 262, 814
MwoI GCNNNNNNNGC 2 cut(s) 106, 262
NciI CCSGG 5 cut(s) 43, 528, 607, 608, 808
NdeII GATC 4 cut(s) 11, 186, 330, 448
NlaIII CATG 4 cut(s) 223, 337, 626, 665
NlaIV GGNNCC 6 cut(s) 121, 280, 301, 611, 612, 646
NmeAIII GCCGAG 1 cut(s) 486
NmuCI GTSAC 1 cut(s) 416
PagI TCATGA 1 cut(s) 219
PaqCI CACCTGC 1 cut(s) 100
PctI GAATGC 1 cut(s) 347
PflMI CCANNNNNTGG 2 cut(s) 201, 272
PkrI GCNGC 5 cut(s) 102, 232, 245, 255, 493
PpuMI RGGWCCY 1 cut(s) 610
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 4 cut(s) 7, 200, 260, 812
PspEI GGTNACC 1 cut(s) 416
PspGI CCWGG 4 cut(s) 7, 200, 260, 812
PspN4I GGNNCC 6 cut(s) 121, 280, 301, 611, 612, 646
PspPI GGNCC 6 cut(s) 119, 204, 610, 798, 810, 816
PspPPI RGGWCCY 1 cut(s) 610
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 2 cut(s) 355, 514
RsaNI GTAC 2 cut(s) 354, 513
SatI GCNGC 5 cut(s) 101, 231, 244, 254, 492
Sau3AI GATC 4 cut(s) 11, 186, 330, 448
Sau96I GGNCC 6 cut(s) 119, 204, 610, 798, 810, 816
ScaI AGTACT 1 cut(s) 355
ScrFI CCNGG 9 cut(s) 9, 43, 202, 262, 528, 607, 608, 808, 814
SduI GDGCHC 3 cut(s) 440, 556, 661
SfaNI GCATC 1 cut(s) 255
SgrAI CRCCGGYG 1 cut(s) 296
SinI GGWCC 2 cut(s) 204, 610
SmaI CCCGGG 1 cut(s) 608
SmlI CTYRAG 2 cut(s) 386, 671
SmoI CTYRAG 2 cut(s) 386, 671
SpeI ACTAGT 2 cut(s) 356, 500
Sse9I AATT 5 cut(s) 158, 269, 571, 713, 820
SsiI CCGC 4 cut(s) 230, 253, 314, 458
SspMI CTAG 2 cut(s) 357, 501
StyD4I CCNGG 9 cut(s) 7, 41, 200, 260, 526, 605, 606, 806, 812
TaaI ACNGT 1 cut(s) 435
TaiI ACGT 1 cut(s) 522
TaqI TCGA 2 cut(s) 57, 339
TasI AATT 5 cut(s) 158, 269, 571, 713, 820
TatI WGTACW 1 cut(s) 353
TauI GCSGC 2 cut(s) 233, 256
TscAI CASTG 3 cut(s) 240, 428, 440
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 3 cut(s) 100, 243, 491
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 3 cut(s) 180, 582, 639
TspGWI ACGGA 1 cut(s) 84
TspMI CCCGGG 1 cut(s) 606
TspRI CASTG 3 cut(s) 240, 428, 440
Van91I CCANNNNNTGG 2 cut(s) 201, 272
VpaK11BI GGWCC 2 cut(s) 204, 610
XagI CCTNNNNNAGG 1 cut(s) 596
XapI RAATTY 4 cut(s) 158, 269, 571, 713
XmaI CCCGGG 1 cut(s) 606
XspI CTAG 2 cut(s) 357, 501
ZrmI AGTACT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.