Rw6G031010

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016233
Physical Location & Seq
Forward (+)
55084532 .. 55086149
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031010.1

Sequence Viewer

Length: 807 bp
ATGCGACCCAACCAGCCAAGCCCCTGTGGGCAGATATCGATGAGGAGGACGTACGTACAAGTGGATTTGGACCTCCTCCTTCTGCCGCCAAAGCAGGTGATTGGCCCCGACGAGAAGGGGATGAAGAAGGTGATTGAATACAAATACAATGAAAAAAGGGTCAGAAGGTCAAGATCACCACCACTCATGAGTAAGGCGGCCGTGGAGCGGCCCGCCTGGCCAAAATTTGGTGGTGCCGTCAATGAAGACGCTGGTGCCAATCTGACAGCAGTTTCCAAAGAGGAGATCATGCTCAAACGCCCCAAGGCTCTTGGCGCCAACAAGGCACAAGAACTACCAAAGAGTACTAGTGGTAATGGAGGAGGCAATACTACTCTCAAGGTTTGTAGGACTTGTGGTAAGAAAGGTGACCACTGGACTGCACAGTGCCCATACAAAGATCTTGCCCCGCAGAGTCGTTCCGGAGATATTAACTCATCCGAGGCAGCCACAACTAGCAAGACTGGTACATACGTTCCTCCGGGTTTGAGAGAAGGAGTGAAGAGGGCTCTGAGGAATGATGAGAATTCAGTTCGGGTGAGCAACCTTTCGGAGGATACTCGGGACCCCGATTTGCACGAACTTTTCAGTCCTTTTGGTGCCATAAGCCGTGCTCATATTGTTCTTGAGAGAGACACCCAAGTTAGCAGAGGATTTGGTTTTGTTAACTTTCTCAGCAAGGAACACGGCCAAAAAGCCATAGACAAACTCAATGGGTATGGTTATGACAATCTCATCCTCTGGGTAGAATGGGCCACGCCTAATTGA

Protein Analysis

268

Amino Acids

29.59

Weight (kDa)

9.5

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 32 - 148 6.8e-22 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 191 - 257 7.3e-17 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 85
Acc36I ACCTGC 1 cut(s) 85
AccB1I GGYRCC 4 cut(s) 233, 254, 314, 638
AccB7I CCANNNNNTGG 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 208
AccIII TCCGGA 1 cut(s) 461
AciI CCGC 5 cut(s) 86, 197, 208, 213, 449
AcoI YGGCCR 3 cut(s) 198, 218, 727
AcsI RAATTY 2 cut(s) 224, 565
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 4 cut(s) 53, 57, 346, 508
AfiI CCNNNNNNNGG 3 cut(s) 207, 227, 592
AgsI TTSAA 1 cut(s) 137
AhlI ACTAGT 1 cut(s) 347
AjnI CCWGG 1 cut(s) 215
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 1 cut(s) 666
Ama87I CYCGRG 1 cut(s) 600
Aor13HI TCCGGA 1 cut(s) 461
AoxI GGCC 6 cut(s) 103, 198, 209, 218, 727, 792
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 2 cut(s) 224, 565
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 5 cut(s) 70, 104, 210, 604, 792
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 5 cut(s) 109, 142, 168, 419, 589
AvaI CYCGRG 1 cut(s) 600
AvaII GGWCC 2 cut(s) 70, 604
BaeGI GKGCMC 1 cut(s) 431
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BalI TGGCCA 1 cut(s) 220
BanI GGYRCC 4 cut(s) 233, 254, 314, 638
BanII GRGCYC 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 655
BbvI GCAGC 1 cut(s) 497
BceAI ACGGC 4 cut(s) 185, 221, 633, 742
BciT130I CCWGG 1 cut(s) 217
BciVI GTATCC 1 cut(s) 589
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 666
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 2 cut(s) 348, 495
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 85
BfuI GTATCC 1 cut(s) 589
BglI GCCNNNNNGGC 2 cut(s) 217, 323
BglII AGATCT 1 cut(s) 439
BisI GCNGC 4 cut(s) 86, 198, 209, 486
BlsI GCNGC 4 cut(s) 87, 199, 210, 487
BmcAI AGTACT 1 cut(s) 346
Bme1390I CCNGG 2 cut(s) 217, 522
Bme18I GGWCC 2 cut(s) 70, 604
BmeT110I CYCGRG 1 cut(s) 600
BmgT120I GGNCC 5 cut(s) 70, 104, 210, 604, 792
BmiI GGNNCC 7 cut(s) 106, 235, 256, 316, 605, 606, 640
BmrFI CCNGG 2 cut(s) 217, 522
BpiI GAAGAC 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 362, 686
BpuMI CCSGG 1 cut(s) 522
Bsa29I ATCGAT 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 201, 303, 480
BsaWI WCCGGW 1 cut(s) 461
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 227, 592
Bse1I ACTGG 2 cut(s) 419, 508
BseAI TCCGGA 1 cut(s) 461
BseBI CCWGG 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 3 cut(s) 201, 303, 480
BseGI GGATG 3 cut(s) 126, 476, 774
BseLI CCNNNNNNNGG 3 cut(s) 207, 227, 592
BseMII CTCAG 2 cut(s) 542, 727
BseNI ACTGG 2 cut(s) 419, 508
BseRI GAGGAG 4 cut(s) 58, 65, 296, 375
BseSI GKGCMC 1 cut(s) 431
BseX3I CGGCCG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 6 cut(s) 105, 200, 211, 220, 729, 794
BshNI GGYRCC 4 cut(s) 233, 254, 314, 638
BshVI ATCGAT 1 cut(s) 38
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 655
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 2 cut(s) 462, 521
BsiWI CGTACG 1 cut(s) 51
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 3 cut(s) 207, 227, 592
BsmAI GTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 617
BsnI GGCC 6 cut(s) 105, 200, 211, 220, 729, 794
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 3 cut(s) 431, 550, 655
Bsp13I TCCGGA 1 cut(s) 461
Bsp143I GATC 3 cut(s) 173, 285, 439
BspACI CCGC 5 cut(s) 86, 197, 208, 213, 449
BspANI GGCC 6 cut(s) 105, 200, 211, 220, 729, 794
BspCNI CTCAG 2 cut(s) 543, 726
BspDI ATCGAT 1 cut(s) 38
BspEI TCCGGA 1 cut(s) 461
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 7 cut(s) 106, 235, 256, 316, 605, 606, 640
BspMI ACCTGC 1 cut(s) 85
BspT107I GGYRCC 4 cut(s) 233, 254, 314, 638
BsrBI CCGCTC 1 cut(s) 208
BsrI ACTGG 2 cut(s) 419, 508
BssECI CCNNGG 3 cut(s) 201, 303, 480
BssMI GATC 3 cut(s) 173, 285, 439
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 217
Bst4CI ACNGT 1 cut(s) 426
Bst6I CTCTTC 1 cut(s) 536
BstACI GRCGYC 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 2 cut(s) 551, 713
BstDSI CCRYGG 1 cut(s) 201
BstEII GGTNACC 1 cut(s) 407
BstENI CCTNNNNNAGG 1 cut(s) 590
BstF5I GGATG 3 cut(s) 126, 476, 774
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 3 cut(s) 176, 288, 442
BstMAI GTCTC 1 cut(s) 666
BstMBI GATC 3 cut(s) 173, 285, 439
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 4 cut(s) 91, 217, 314, 323
BstNI CCWGG 1 cut(s) 217
BstPI GGTNACC 1 cut(s) 407
BstSCI CCNGG 2 cut(s) 215, 520
BstSLI GKGCMC 1 cut(s) 431
BstSNI TACGTA 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 497
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BstZI CGGCCG 1 cut(s) 198
Bsu15I ATCGAT 1 cut(s) 38
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 6 cut(s) 105, 200, 211, 220, 729, 794
BsuTUI ATCGAT 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 3 cut(s) 126, 476, 774
BtsIMutI CAGTG 2 cut(s) 412, 431
BveI ACCTGC 1 cut(s) 85
Cac8I GCNNGC 1 cut(s) 213
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 5 cut(s) 70, 104, 210, 604, 792
ClaI ATCGAT 1 cut(s) 38
CseI GACGC 1 cut(s) 257
Csp6I GTAC 4 cut(s) 52, 56, 345, 507
CviAII CATG 2 cut(s) 187, 289
CviQI GTAC 4 cut(s) 52, 56, 345, 507
DdeI CTNAG 2 cut(s) 551, 713
DinI GGCGCC 1 cut(s) 316
DpnI GATC 3 cut(s) 175, 287, 441
DpnII GATC 3 cut(s) 173, 285, 439
EaeI YGGCCR 3 cut(s) 198, 218, 727
EagI CGGCCG 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
EclXI CGGCCG 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 550
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 2 cut(s) 70, 604
Eco52I CGGCCG 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 600
Eco91I GGTNACC 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 604
EcoO65I GGTNACC 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 215
EcoRV GATATC 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 303
EcoT38I GRGCYC 1 cut(s) 550
EgeI GGCGCC 1 cut(s) 316
EheI GGCGCC 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 2 cut(s) 190, 292
FaiI YATR 9 cut(s) 188, 290, 433, 511, 644, 657, 740, 759, 765
FaqI GGGAC 1 cut(s) 617
FatI CATG 2 cut(s) 186, 288
FauI CCCGC 2 cut(s) 220, 456
Fnu4HI GCNGC 4 cut(s) 86, 198, 209, 486
FokI GGATG 3 cut(s) 133, 463, 761
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 4 cut(s) 86, 198, 209, 486
FspBI CTAG 2 cut(s) 348, 495
GlaI GCGC 1 cut(s) 316
GluI GCNGC 4 cut(s) 86, 198, 209, 486
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 6 cut(s) 105, 200, 211, 220, 729, 794
HapII CCGG 2 cut(s) 462, 521
HgaI GACGC 1 cut(s) 257
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 2 cut(s) 190, 292
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 1 cut(s) 454
HpaI GTTAAC 1 cut(s) 706
HpaII CCGG 2 cut(s) 462, 521
HphI GGTGA 5 cut(s) 109, 142, 168, 419, 589
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 5 cut(s) 164, 264, 481, 552, 592
Hpy188III TCNNGA 5 cut(s) 171, 187, 462, 602, 665
Hpy8I GTNNAC 1 cut(s) 706
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 5 cut(s) 89, 109, 121, 159, 527
HpyCH4III ACNGT 1 cut(s) 426
HpyCH4IV ACGT 3 cut(s) 50, 54, 513
HpyCH4V TGCA 2 cut(s) 422, 616
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 217, 314, 323
HpyF3I CTNAG 2 cut(s) 551, 713
HpySE526I ACGT 3 cut(s) 50, 54, 513
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 2 cut(s) 190, 292
HspAI GCGC 1 cut(s) 315
KasI GGCGCC 1 cut(s) 314
KflI GGGWCCC 1 cut(s) 604
Kpn2I TCCGGA 1 cut(s) 461
KspAI GTTAAC 1 cut(s) 706
Kzo9I GATC 3 cut(s) 173, 285, 439
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 1 cut(s) 497
MaeI CTAG 2 cut(s) 348, 495
MaeII ACGT 3 cut(s) 50, 54, 513
MaeIII GTNAC 1 cut(s) 407
MalI GATC 3 cut(s) 175, 287, 441
MbiI CCGCTC 1 cut(s) 208
MboI GATC 3 cut(s) 173, 285, 439
MboII GAAGA 3 cut(s) 136, 257, 553
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 3 cut(s) 431, 550, 655
MlsI TGGCCA 1 cut(s) 220
MluCI AATT 3 cut(s) 224, 565, 802
MluNI TGGCCA 1 cut(s) 220
Mly113I GGCGCC 1 cut(s) 315
MlyI GAGTC 1 cut(s) 463
Mox20I TGGCCA 1 cut(s) 220
MroI TCCGGA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 220
MseI TTAA 2 cut(s) 471, 705
Msp20I TGGCCA 1 cut(s) 220
MspI CCGG 2 cut(s) 462, 521
MspR9I CCNGG 2 cut(s) 217, 522
MvaI CCWGG 1 cut(s) 217
MwoI GCNNNNNNNGC 4 cut(s) 91, 217, 314, 323
NarI GGCGCC 1 cut(s) 315
NciI CCSGG 1 cut(s) 522
NdeII GATC 3 cut(s) 173, 285, 439
NlaIII CATG 2 cut(s) 190, 292
NlaIV GGNNCC 7 cut(s) 106, 235, 256, 316, 605, 606, 640
NmuCI GTSAC 1 cut(s) 407
PagI TCATGA 1 cut(s) 186
PaqCI CACCTGC 1 cut(s) 85
Pfl23II CGTACG 1 cut(s) 51
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 4 cut(s) 87, 199, 210, 487
PleI GAGTC 1 cut(s) 462
PluTI GGCGCC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 462
Ppu21I YACGTR 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 604
Psp5II RGGWCCY 1 cut(s) 604
Psp6I CCWGG 1 cut(s) 215
PspEI GGTNACC 1 cut(s) 407
PspGI CCWGG 1 cut(s) 215
PspLI CGTACG 1 cut(s) 51
PspN4I GGNNCC 7 cut(s) 106, 235, 256, 316, 605, 606, 640
PspPI GGNCC 5 cut(s) 70, 104, 210, 604, 792
PspPPI RGGWCCY 1 cut(s) 604
PsuI RGATCY 1 cut(s) 439
RsaI GTAC 4 cut(s) 53, 57, 346, 508
RsaNI GTAC 4 cut(s) 52, 56, 345, 507
SaqAI TTAA 2 cut(s) 471, 705
SatI GCNGC 4 cut(s) 86, 198, 209, 486
Sau3AI GATC 3 cut(s) 173, 285, 439
Sau96I GGNCC 5 cut(s) 70, 104, 210, 604, 792
ScaI AGTACT 1 cut(s) 346
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 2 cut(s) 217, 522
SduI GDGCHC 3 cut(s) 431, 550, 655
SfiI GGCCNNNNNGGCC 1 cut(s) 217
SfoI GGCGCC 1 cut(s) 316
SinI GGWCC 2 cut(s) 70, 604
SmlI CTYRAG 2 cut(s) 377, 665
SmoI CTYRAG 2 cut(s) 377, 665
SnaBI TACGTA 1 cut(s) 55
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 3 cut(s) 224, 565, 802
SsiI CCGC 5 cut(s) 86, 197, 208, 213, 449
SspDI GGCGCC 1 cut(s) 314
SspMI CTAG 2 cut(s) 348, 495
StyD4I CCNGG 2 cut(s) 215, 520
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 426
TaiI ACGT 3 cut(s) 53, 57, 516
TaqI TCGA 1 cut(s) 38
TasI AATT 3 cut(s) 224, 565, 802
TatI WGTACW 1 cut(s) 344
TauI GCSGC 3 cut(s) 88, 200, 211
Tru1I TTAA 2 cut(s) 471, 705
Tru9I TTAA 2 cut(s) 471, 705
TscAI CASTG 2 cut(s) 419, 431
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 137, 165, 258
TspRI CASTG 2 cut(s) 419, 431
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 2 cut(s) 70, 604
XagI CCTNNNNNAGG 1 cut(s) 590
XapI RAATTY 2 cut(s) 224, 565
XspI CTAG 2 cut(s) 348, 495
ZrmI AGTACT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.