Rh6CG369900

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0016233
Physical Location & Seq
Forward (+)
55711657 .. 55712313
657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG369900.1

Sequence Viewer

Length: 657 bp
ATGAAAAGGGTCAGAAGGTCAAGATCACCACCACTCATGAGTAAGGCGGCCGTGGAGCGGCGCGCCTGGCCAAAATTTGGTGGTGCCGTCAATGAAGACGCTGGTGCCAATCTTACAGCAGTTTCCAAAGAGGAGATCATGCTCAAACGCCCCAAGGCTCTTGTCGCCAATAAGGCACAAGAACTACCAAAGAGTACTAGTGGTAATGGAGGAGGCAATACTACTCTCAAGGTTTGTAGGACTTGTGGTAAGAAAGGTGACCACTGGACTGCACAGTGCCCATACAAAGATCTTGCCCCGCAGAGTCGTTCCGGAGATATTAGCTCATCCGAGGCAGCCACAACTAGCAAGACTGGTACATACGTTCCTCCGGGTTTGAGAGAAGGAGTGAATAGGGCTCTGAGGAATGATGAGAATTCAGTTCGGGTGAGCAACCTTTTGGAGGATACCCGGGACCCCAATTTGCATGAACTTTTCAGTCCTTTTGGTGACATAAGCCGTGCTCATGTTGTTCTTGAGAGAGACACCCAAGTTAGCAGAGGATTTGGTTTTGTTAACTTTCTCAGCAAGGAACACGGCCAAAAAGCCATAGACAAATTCAATGGGTATGGTTATGACAATCTCATCCTCCGGGTAGAATGGGCCACGCCTAATTGA

Protein Analysis

218

Amino Acids

23.94

Weight (kDa)

9.68

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 2 - 98 1.7e-19 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 141 - 207 2.5e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06650
rosa_chinensis RchiOBHm_Chr2g0092401 RchiOBHm_Chr6g0294311
rosa_laevigata RLG00000016307
rosa_multiflora Rmu_co8481273.1_g000001 Rmu_sc0001195.1_g000020
rosa_roxburghii Rroxscaffold_2G00149150
rosa_rugosa Rorug02G0025800
rosa_samantha Rh2AG071800 Rh2BG071800 Rh2CG073200 Rh2DG070300 Rh6CG369900
rosa_wichuraiana Rw2G006080 Rw6G031010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 104
AccB7I CCANNNNNTGG 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 58
AccII CGCG 1 cut(s) 63
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 3 cut(s) 47, 58, 299
AcoI YGGCCR 3 cut(s) 48, 68, 577
AcsI RAATTY 3 cut(s) 74, 415, 596
AfaI GTAC 2 cut(s) 196, 358
AfiI CCNNNNNNNGG 3 cut(s) 57, 77, 442
AgsI TTSAA 1 cut(s) 601
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 1 cut(s) 324
AluI AGCT 1 cut(s) 324
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 1 cut(s) 516
Ama87I CYCGRG 1 cut(s) 450
Aor13HI TCCGGA 1 cut(s) 311
AoxI GGCC 4 cut(s) 48, 68, 577, 642
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 3 cut(s) 74, 415, 596
AscI GGCGCGCC 1 cut(s) 61
AspLEI GCGC 2 cut(s) 63, 65
AspS9I GGNCC 2 cut(s) 454, 642
AsuC2I CCSGG 4 cut(s) 372, 451, 452, 632
AsuHPI GGTGA 4 cut(s) 18, 269, 439, 500
AvaI CYCGRG 1 cut(s) 450
AvaII GGWCC 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 281
BaeI ACNNNNGTAYC 2 cut(s) 348, 381
BalI TGGCCA 1 cut(s) 70
BanI GGYRCC 2 cut(s) 83, 104
BanII GRGCYC 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 1 cut(s) 347
BceAI ACGGC 4 cut(s) 35, 71, 483, 592
BciT130I CCWGG 1 cut(s) 67
BciVI GTATCC 1 cut(s) 439
BcnI CCSGG 4 cut(s) 372, 451, 452, 632
BcoDI GTCTC 1 cut(s) 516
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 198, 345
BfuI GTATCC 1 cut(s) 439
BglI GCCNNNNNGGC 1 cut(s) 173
BglII AGATCT 1 cut(s) 289
BisI GCNGC 3 cut(s) 48, 59, 336
BlsI GCNGC 3 cut(s) 49, 60, 337
BmcAI AGTACT 1 cut(s) 196
Bme1390I CCNGG 5 cut(s) 67, 372, 451, 452, 632
Bme18I GGWCC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 450
BmgT120I GGNCC 2 cut(s) 454, 642
BmiI GGNNCC 4 cut(s) 85, 106, 455, 456
BmrFI CCNGG 5 cut(s) 67, 372, 451, 452, 632
BpiI GAAGAC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 212, 536
BpuMI CCSGG 4 cut(s) 372, 451, 452, 632
BsaJI CCNNGG 4 cut(s) 51, 153, 330, 450
BsaWI WCCGGW 1 cut(s) 311
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 77, 442
Bse1I ACTGG 2 cut(s) 269, 358
BseAI TCCGGA 1 cut(s) 311
BseBI CCWGG 1 cut(s) 67
BseDI CCNNGG 4 cut(s) 51, 153, 330, 450
BseGI GGATG 2 cut(s) 326, 624
BseLI CCNNNNNNNGG 3 cut(s) 57, 77, 442
BseMII CTCAG 2 cut(s) 392, 577
BseNI ACTGG 2 cut(s) 269, 358
BsePI GCGCGC 1 cut(s) 61
BseRI GAGGAG 2 cut(s) 146, 225
BseSI GKGCMC 1 cut(s) 281
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 63
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 4 cut(s) 50, 70, 579, 644
BshNI GGYRCC 2 cut(s) 83, 104
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 505
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 4 cut(s) 312, 371, 451, 631
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 3 cut(s) 57, 77, 442
BsmAI GTCTC 1 cut(s) 516
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 4 cut(s) 50, 70, 579, 644
BsoBI CYCGRG 1 cut(s) 450
Bsp1286I GDGCHC 3 cut(s) 281, 400, 505
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 3 cut(s) 23, 135, 289
BspACI CCGC 3 cut(s) 47, 58, 299
BspANI GGCC 4 cut(s) 50, 70, 579, 644
BspCNI CTCAG 2 cut(s) 393, 576
BspEI TCCGGA 1 cut(s) 311
BspFNI CGCG 1 cut(s) 63
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 4 cut(s) 85, 106, 455, 456
BspT107I GGYRCC 2 cut(s) 83, 104
BsrBI CCGCTC 1 cut(s) 58
BsrI ACTGG 2 cut(s) 269, 358
BssECI CCNNGG 4 cut(s) 51, 153, 330, 450
BssHII GCGCGC 1 cut(s) 61
BssMI GATC 3 cut(s) 23, 135, 289
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 2 cut(s) 401, 563
BstDSI CCRYGG 1 cut(s) 51
BstEII GGTNACC 1 cut(s) 257
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 2 cut(s) 326, 624
BstFNI CGCG 1 cut(s) 63
BstHHI GCGC 2 cut(s) 63, 65
BstKTI GATC 3 cut(s) 26, 138, 292
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 3 cut(s) 23, 135, 289
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 3 cut(s) 67, 164, 173
BstNI CCWGG 1 cut(s) 67
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 5 cut(s) 65, 370, 449, 450, 630
BstSLI GKGCMC 1 cut(s) 281
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 347
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BstZI CGGCCG 1 cut(s) 48
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 4 cut(s) 50, 70, 579, 644
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 326, 624
BtsIMutI CAGTG 2 cut(s) 262, 281
Cac8I GCNNGC 1 cut(s) 63
CciI TCATGA 1 cut(s) 36
CfoI GCGC 2 cut(s) 63, 65
Cfr13I GGNCC 2 cut(s) 454, 642
Cfr9I CCCGGG 1 cut(s) 450
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 195, 357
CviAII CATG 4 cut(s) 37, 139, 467, 506
CviQI GTAC 2 cut(s) 195, 357
DdeI CTNAG 2 cut(s) 401, 563
DpnI GATC 3 cut(s) 25, 137, 291
DpnII GATC 3 cut(s) 23, 135, 289
EaeI YGGCCR 3 cut(s) 48, 68, 577
EagI CGGCCG 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 454
Eco52I CGGCCG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 450
Eco91I GGTNACC 1 cut(s) 257
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 454
EcoO65I GGTNACC 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 4 cut(s) 40, 142, 470, 509
FaqI GGGAC 1 cut(s) 467
FatI CATG 4 cut(s) 36, 138, 466, 505
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 3 cut(s) 48, 59, 336
FokI GGATG 2 cut(s) 313, 611
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 3 cut(s) 48, 59, 336
FspBI CTAG 2 cut(s) 198, 345
GlaI GCGC 2 cut(s) 62, 64
GluI GCNGC 3 cut(s) 48, 59, 336
HaeIII GGCC 4 cut(s) 50, 70, 579, 644
HapII CCGG 4 cut(s) 312, 371, 451, 631
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 63, 65
Hin1II CATG 4 cut(s) 40, 142, 470, 509
Hin6I GCGC 2 cut(s) 61, 63
HinP1I GCGC 2 cut(s) 61, 63
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 1 cut(s) 304
HpaI GTTAAC 1 cut(s) 556
HpaII CCGG 4 cut(s) 312, 371, 451, 631
HphI GGTGA 4 cut(s) 18, 269, 439, 500
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 3 cut(s) 14, 331, 402
Hpy188III TCNNGA 4 cut(s) 21, 37, 312, 515
Hpy8I GTNNAC 1 cut(s) 556
HpyAV CCTTC 2 cut(s) 9, 377
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 2 cut(s) 272, 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 164, 173
HpyF3I CTNAG 2 cut(s) 401, 563
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 4 cut(s) 40, 142, 470, 509
HspAI GCGC 2 cut(s) 61, 63
KflI GGGWCCC 1 cut(s) 454
Kpn2I TCCGGA 1 cut(s) 311
KspAI GTTAAC 1 cut(s) 556
Kzo9I GATC 3 cut(s) 23, 135, 289
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 9 cut(s) 52, 79, 87, 250, 325, 339, 384, 464, 644
Lsp1109I GCAGC 1 cut(s) 347
MaeI CTAG 2 cut(s) 198, 345
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 257, 488
MalI GATC 3 cut(s) 25, 137, 291
MbiI CCGCTC 1 cut(s) 58
MboI GATC 3 cut(s) 23, 135, 289
MboII GAAGA 1 cut(s) 107
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 3 cut(s) 281, 400, 505
MlsI TGGCCA 1 cut(s) 70
MluCI AATT 5 cut(s) 74, 415, 460, 596, 652
MluNI TGGCCA 1 cut(s) 70
MlyI GAGTC 1 cut(s) 313
MnlI CCTC 9 cut(s) 124, 203, 206, 325, 378, 396, 436, 533, 638
Mox20I TGGCCA 1 cut(s) 70
MroI TCCGGA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 70
MseI TTAA 1 cut(s) 555
Msp20I TGGCCA 1 cut(s) 70
MspI CCGG 4 cut(s) 312, 371, 451, 631
MspR9I CCNGG 5 cut(s) 67, 372, 451, 452, 632
MvaI CCWGG 1 cut(s) 67
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 3 cut(s) 67, 164, 173
NciI CCSGG 4 cut(s) 372, 451, 452, 632
NdeII GATC 3 cut(s) 23, 135, 289
NlaIII CATG 4 cut(s) 40, 142, 470, 509
NlaIV GGNNCC 4 cut(s) 85, 106, 455, 456
NmuCI GTSAC 2 cut(s) 257, 488
PagI TCATGA 1 cut(s) 36
PalAI GGCGCGCC 1 cut(s) 61
PauI GCGCGC 1 cut(s) 61
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 3 cut(s) 49, 60, 337
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
PpuMI RGGWCCY 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 454
Psp6I CCWGG 1 cut(s) 65
PspEI GGTNACC 1 cut(s) 257
PspGI CCWGG 1 cut(s) 65
PspN4I GGNNCC 4 cut(s) 85, 106, 455, 456
PspPI GGNCC 2 cut(s) 454, 642
PspPPI RGGWCCY 1 cut(s) 454
PsuI RGATCY 1 cut(s) 289
PteI GCGCGC 1 cut(s) 61
RsaI GTAC 2 cut(s) 196, 358
RsaNI GTAC 2 cut(s) 195, 357
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 3 cut(s) 48, 59, 336
Sau3AI GATC 3 cut(s) 23, 135, 289
Sau96I GGNCC 2 cut(s) 454, 642
ScaI AGTACT 1 cut(s) 196
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 5 cut(s) 67, 372, 451, 452, 632
SduI GDGCHC 3 cut(s) 281, 400, 505
SetI ASST 6 cut(s) 20, 234, 259, 326, 366, 438
SgsI GGCGCGCC 1 cut(s) 61
SinI GGWCC 1 cut(s) 454
SmaI CCCGGG 1 cut(s) 452
SmlI CTYRAG 2 cut(s) 227, 515
SmoI CTYRAG 2 cut(s) 227, 515
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 5 cut(s) 74, 415, 460, 596, 652
SsiI CCGC 3 cut(s) 47, 58, 299
SspMI CTAG 2 cut(s) 198, 345
StyD4I CCNGG 5 cut(s) 65, 370, 449, 450, 630
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 1 cut(s) 276
TaiI ACGT 1 cut(s) 366
TasI AATT 5 cut(s) 74, 415, 460, 596, 652
TatI WGTACW 1 cut(s) 194
TauI GCSGC 2 cut(s) 50, 61
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 2 cut(s) 269, 281
TseFI GTSAC 2 cut(s) 257, 488
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 2 cut(s) 257, 488
TspDTI ATGAA 3 cut(s) 17, 108, 483
TspMI CCCGGG 1 cut(s) 450
TspRI CASTG 2 cut(s) 269, 281
Van91I CCANNNNNTGG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 454
XagI CCTNNNNNAGG 1 cut(s) 440
XapI RAATTY 3 cut(s) 74, 415, 596
XmaI CCCGGG 1 cut(s) 450
XspI CTAG 2 cut(s) 198, 345
ZrmI AGTACT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.