FvH4_1g07080

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014230
Physical Location & Seq
Forward (+)
3745067 .. 3746141
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07080.t1

Sequence Viewer

Length: 429 bp
ATGGCTGACGTCAGCCTATTTGGGCACCCATTCAGGCGATTCCTATGGAGTCCTCAGCACTGGTCTGGCTCCACAGCTCTCATGGACTGGCTTGAATCACCAACTGCCCATATATTCAAGTTCAATGTTCCAGGTTTTAGGAAGGAAGAGATAAAGGTGCAGATAGAAGAAGGGAACATTTTGCAGATAAAAGGGGAAGGTGGGAAAGAGGAAGCCAATGCAAAAGACACTGTTTGGCACGTGGCGGAGAGAGGGACTGCCGGGAAATCAGTAGCTGAGTTTTATCGGGAAATTGAGTTGCCGGAAAATGTGAAGGTGGATCAGATTAAAGCTCAGGTGGAGAATGGTGTGCTCACCATTCTTGTCCCAAAAGATGCCACCCCTAAACCATCTAGAGTGCGAAATATCAATATTACTAGCAAGCTCTAA

Protein Analysis

143

Amino Acids

16.02

Weight (kDa)

6.92

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 29 - 139 3.3e-22 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37670
fragaria_vesca FvH4_1g07080
malus_domestica MD09G1271100.v1.1 MD17G1269200.v1.1
prunus_persica Prupe.3G017400_v2.0.a1
pyrus_communis pycom09g18210 pycom17g26790
rosa_chinensis RchiOBHm_Chr3g0491101
rosa_laevigata RLG00000022863
rosa_multiflora Rmu_sc0005038.1_g000022
rosa_roxburghii Rroxscaffold_6G00393200
rosa_rugosa Rorug03G0246100
rosa_samantha Rh3AG296300 Rh3BG332300 Rh3CG329600 Rh3DG330100
rosa_wichuraiana Rw3G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 24
AciI CCGC 1 cut(s) 245
AclWI GGATC 1 cut(s) 327
AcvI CACGTG 1 cut(s) 241
AcyI GRCGYC 1 cut(s) 9
AgsI TTSAA 3 cut(s) 95, 118, 124
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 4 cut(s) 77, 275, 332, 424
AluI AGCT 4 cut(s) 77, 275, 332, 424
Alw21I GWGCWC 1 cut(s) 354
AlwI GGATC 1 cut(s) 327
AlwNI CAGNNNCTG 1 cut(s) 275
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 2 cut(s) 90, 346
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 1 cut(s) 24
BbrPI CACGTG 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 354
BbvCI CCTCAGC 1 cut(s) 54
BccI CCATC 1 cut(s) 397
BciT130I CCWGG 1 cut(s) 132
BcnI CCSGG 1 cut(s) 262
BfaI CTAG 2 cut(s) 393, 417
Bme1390I CCNGG 2 cut(s) 132, 262
BmiI GGNNCC 2 cut(s) 26, 70
BmrFI CCNGG 2 cut(s) 132, 262
BmsI GCATC 1 cut(s) 364
Bpu10I CCTNAGC 2 cut(s) 54, 333
BpuMI CCSGG 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 1 cut(s) 9
Bse1I ACTGG 2 cut(s) 65, 92
BseBI CCWGG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 68, 267, 347
BseNI ACTGG 2 cut(s) 65, 92
BseSI GKGCMC 1 cut(s) 27
BsgI GTGCAG 1 cut(s) 179
BshNI GGYRCC 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 2 cut(s) 261, 302
BslFI GGGAC 2 cut(s) 268, 350
BsmFI GGGAC 2 cut(s) 268, 350
Bsp1286I GDGCHC 2 cut(s) 27, 354
Bsp143I GATC 1 cut(s) 319
BspACI CCGC 1 cut(s) 245
BspCNI CTCAG 3 cut(s) 67, 268, 346
BspLI GGNNCC 2 cut(s) 26, 70
BspPI GGATC 1 cut(s) 327
BspT107I GGYRCC 1 cut(s) 24
BsrI ACTGG 2 cut(s) 65, 92
BssMI GATC 1 cut(s) 319
BssNI GRCGYC 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 9
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 3 cut(s) 54, 276, 333
BstKTI GATC 1 cut(s) 322
BstMBI GATC 1 cut(s) 319
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 130, 260
BstSLI GKGCMC 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 58, 228
Cac8I GCNNGC 1 cut(s) 422
CaiI CAGNNNCTG 1 cut(s) 275
CviAII CATG 1 cut(s) 82
CviJI RGCY 9 cut(s) 5, 15, 69, 77, 91, 215, 275, 332, 424
CviKI_1 RGCY 9 cut(s) 5, 15, 69, 77, 91, 215, 275, 332, 424
DdeI CTNAG 3 cut(s) 54, 276, 333
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 260
Eco72I CACGTG 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 85
FaiI YATR 4 cut(s) 46, 83, 111, 113
FaqI GGGAC 2 cut(s) 268, 350
FatI CATG 1 cut(s) 81
FspBI CTAG 2 cut(s) 393, 417
HapII CCGG 2 cut(s) 261, 302
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 1 cut(s) 85
HinfI GANTC 3 cut(s) 39, 49, 95
HpaII CCGG 2 cut(s) 261, 302
HphI GGTGA 2 cut(s) 90, 346
Hpy188I TCNGA 1 cut(s) 324
Hpy188III TCNNGA 2 cut(s) 287, 393
HpyAV CCTTC 4 cut(s) 136, 164, 191, 307
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 2 cut(s) 9, 240
HpyCH4V TGCA 3 cut(s) 160, 184, 221
HpyF3I CTNAG 3 cut(s) 54, 276, 333
HpySE526I ACGT 2 cut(s) 9, 240
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 1 cut(s) 85
Kzo9I GATC 1 cut(s) 319
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 9 cut(s) 19, 46, 51, 73, 117, 144, 274, 315, 320
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 393, 417
MaeII ACGT 2 cut(s) 9, 240
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 2 cut(s) 158, 179
MhlI GDGCHC 2 cut(s) 27, 354
MluCI AATT 1 cut(s) 291
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 3 cut(s) 63, 202, 245
MseI TTAA 1 cut(s) 327
MspI CCGG 2 cut(s) 261, 302
MspR9I CCNGG 2 cut(s) 132, 262
MvaI CCWGG 1 cut(s) 132
NciI CCSGG 1 cut(s) 262
NdeII GATC 1 cut(s) 319
NlaIII CATG 1 cut(s) 85
NlaIV GGNNCC 2 cut(s) 26, 70
PfeI GAWTC 2 cut(s) 39, 95
PleI GAGTC 1 cut(s) 57
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 1 cut(s) 57
Ppu21I YACGTR 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 130
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 26, 70
PstNI CAGNNNCTG 1 cut(s) 275
SaqAI TTAA 1 cut(s) 327
Sau3AI GATC 1 cut(s) 319
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 2 cut(s) 132, 262
SduI GDGCHC 2 cut(s) 27, 354
SfaNI GCATC 1 cut(s) 364
Sse9I AATT 1 cut(s) 291
SsiI CCGC 1 cut(s) 245
SspI AATATT 1 cut(s) 412
SspMI CTAG 2 cut(s) 393, 417
StyD4I CCNGG 2 cut(s) 130, 260
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 2 cut(s) 12, 243
TasI AATT 1 cut(s) 291
TfiI GAWTC 2 cut(s) 39, 95
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 2 cut(s) 65, 235
TspRI CASTG 2 cut(s) 65, 235
XbaI TCTAGA 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XspI CTAG 2 cut(s) 393, 417
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.