MD09G1271100.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014230
Physical Location & Seq
Forward (+)
34531591 .. 34533232
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1271100.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGCAGCTGATAGCATATTTGGGTACCCGTTCAGGCGCCTACTCTGGAGCCCTCCGGTTTTCCGTGAATGGTCAGGATCAACGGCCCACATGGACTGGCTTGAATCCCCTAATGCCCACATATTCAAGATCAATGTTCCAGGTTTTAGAAAAGAGGACATAAAAGTGCAGATAGAAGAAGGGAACATTTTACAGATAAAAGGGGAAGGCGGGAAAGAAAGAGAGGAGGCTCATGCAGCAAAAGACACTGTTTGGCACGTCGCGGAGAGGGGGACGGGAAAAGGAGACTTTTCTCGGGAAATCGAGTTGCCGGAAAATGTGAAGGTGGATCAGATAAAAGCTCAGGTTGAGAATGGTGTTCTCACTATTGTTGTCCCAAAAGATTCGACCCCCAAACCATCCAGAGTGCGAAACATCAATATCACTAGCAAGCTCTAA

Protein Analysis

146

Amino Acids

16.36

Weight (kDa)

6.92

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 142 2.2e-23 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37670
fragaria_vesca FvH4_1g07080
malus_domestica MD09G1271100.v1.1 MD17G1269200.v1.1
prunus_persica Prupe.3G017400_v2.0.a1
pyrus_communis pycom09g18210 pycom17g26790
rosa_chinensis RchiOBHm_Chr3g0491101
rosa_laevigata RLG00000022863
rosa_multiflora Rmu_sc0005038.1_g000022
rosa_roxburghii Rroxscaffold_6G00393200
rosa_rugosa Rorug03G0246100
rosa_samantha Rh3AG296300 Rh3BG332300 Rh3CG329600 Rh3DG330100
rosa_wichuraiana Rw3G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 2 cut(s) 24, 36
AccII CGCG 1 cut(s) 263
AciI CCGC 2 cut(s) 210, 263
AclWI GGATC 2 cut(s) 85, 336
AcyI GRCGYC 1 cut(s) 37
AfaI GTAC 1 cut(s) 26
AgsI TTSAA 2 cut(s) 104, 127
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 3 cut(s) 8, 341, 433
AluI AGCT 3 cut(s) 8, 341, 433
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 2 cut(s) 85, 336
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 2 cut(s) 5, 236
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 294
BanI GGYRCC 2 cut(s) 24, 36
BanII GRGCYC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 17, 248
BccI CCATC 1 cut(s) 406
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 279
BfaI CTAG 1 cut(s) 426
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 2 cut(s) 6, 237
BlsI GCNGC 2 cut(s) 7, 238
Bme1390I CCNGG 1 cut(s) 141
BmeT110I CYCGRG 1 cut(s) 294
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 3 cut(s) 26, 38, 50
BmrFI CCNGG 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 37
BsaWI WCCGGW 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 398
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 17, 248
BsgI GTGCAG 1 cut(s) 188
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 2 cut(s) 24, 36
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 2 cut(s) 56, 311
BslFI GGGAC 2 cut(s) 286, 359
BsmAI GTCTC 1 cut(s) 279
BsmFI GGGAC 2 cut(s) 286, 359
BsnI GGCC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 3 cut(s) 77, 129, 328
BspACI CCGC 2 cut(s) 210, 263
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 355
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 3 cut(s) 26, 38, 50
BspPI GGATC 2 cut(s) 85, 336
BspT107I GGYRCC 2 cut(s) 24, 36
BsrI ACTGG 1 cut(s) 101
BssMI GATC 3 cut(s) 77, 129, 328
BssNI GRCGYC 1 cut(s) 37
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 250
BstACI GRCGYC 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 431
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 398
BstFNI CGCG 1 cut(s) 263
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 3 cut(s) 80, 132, 331
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 3 cut(s) 77, 129, 328
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 17, 248
BsuRI GGCC 1 cut(s) 86
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 431
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 25
CviAII CATG 2 cut(s) 91, 233
CviJI RGCY 7 cut(s) 8, 51, 86, 100, 230, 341, 433
CviKI_1 RGCY 7 cut(s) 8, 51, 86, 100, 230, 341, 433
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 342
DinI GGCGCC 1 cut(s) 38
DpnI GATC 3 cut(s) 79, 131, 330
DpnII GATC 3 cut(s) 77, 129, 328
Eco24I GRGCYC 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 38
EheI GGCGCC 1 cut(s) 38
FaeI CATG 2 cut(s) 94, 236
FaiI YATR 5 cut(s) 17, 92, 122, 161, 234
FaqI GGGAC 2 cut(s) 286, 359
FatI CATG 2 cut(s) 90, 232
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 2 cut(s) 6, 237
FokI GGATG 1 cut(s) 385
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 6, 237
FspBI CTAG 1 cut(s) 426
GlaI GCGC 1 cut(s) 38
GluI GCNGC 2 cut(s) 6, 237
GsuI CTGGAG 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 1 cut(s) 86
HapII CCGG 2 cut(s) 56, 311
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 2 cut(s) 94, 236
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 2 cut(s) 104, 383
HpaII CCGG 2 cut(s) 56, 311
Hpy188I TCNGA 1 cut(s) 333
Hpy188III TCNNGA 5 cut(s) 46, 75, 127, 296, 402
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 3 cut(s) 173, 200, 316
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 169, 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 2 cut(s) 94, 236
HspAI GCGC 1 cut(s) 37
KasI GGCGCC 1 cut(s) 36
KpnI GGTACC 1 cut(s) 28
Kzo9I GATC 3 cut(s) 77, 129, 328
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 2 cut(s) 17, 248
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 258
MalI GATC 3 cut(s) 79, 131, 330
MboI GATC 3 cut(s) 77, 129, 328
MboII GAAGA 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 53
Mly113I GGCGCC 1 cut(s) 37
MnlI CCTC 5 cut(s) 63, 148, 217, 220, 261
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 56, 311
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 236
NarI GGCGCC 1 cut(s) 37
NdeII GATC 3 cut(s) 77, 129, 328
NlaIII CATG 2 cut(s) 94, 236
NlaIV GGNNCC 3 cut(s) 26, 38, 50
PfeI GAWTC 2 cut(s) 104, 383
PkrI GCNGC 2 cut(s) 7, 238
PluTI GGCGCC 1 cut(s) 40
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 26, 38, 50
PspPI GGNCC 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 164
SatI GCNGC 2 cut(s) 6, 237
Sau3AI GATC 3 cut(s) 77, 129, 328
Sau96I GGNCC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 1 cut(s) 53
SetI ASST 7 cut(s) 10, 145, 261, 327, 343, 348, 435
SfoI GGCGCC 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 164
SsiI CCGC 2 cut(s) 210, 263
SspDI GGCGCC 1 cut(s) 36
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 303, 386
TfiI GAWTC 2 cut(s) 104, 383
TscAI CASTG 1 cut(s) 253
TseI GCWGC 2 cut(s) 5, 236
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 1 cut(s) 253
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.