Rh3AG296300

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0014230
Physical Location & Seq
Reverse (-)
36148139 .. 36155532
7394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG296300.1

Sequence Viewer

Length: 438 bp
ATGGCTGACGGCCTATTTGGGCACCCATTCAGGCGATTCCTCTGGAGTCCTCCAGTTTTCCGAGAGCGCTGGTCCGGTTCTACGGCACTCATGGACTGGCTTGAATCCCCAACTGCCCACATATTCAAATTCAATGTTCCAGGTTTTAGGAAGGAAGAGATAAAGGTGCAGATCGAAGAAGGCAACATTTTGCAGATAAAAGGGGAAGGTGGGAAAGAGGAGGCCAATGGGAAAGACATTGTTTGGCACGTGGCGGAGAGAGGGACGGCCGGGAAATCATTACCAGAGTTTTCTCGGGAAATTGAACTGCCGGATAATGTGAAGGTGGATCAGATTAAAGCTCAGGTGGAGAATGGTGTGCTCACCATTCTTGTACCAAAAGATGCTACTCCTAAACCATCCAGGGTGCGAAATATCAATATTACCAGCAAGCTCTAA

Protein Analysis

145

Amino Acids

16.36

Weight (kDa)

8.01

Isoelectric Point (pI)

62.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 142 6.4e-22 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37670
fragaria_vesca FvH4_1g07080
malus_domestica MD09G1271100.v1.1 MD17G1269200.v1.1
prunus_persica Prupe.3G017400_v2.0.a1
pyrus_communis pycom09g18210 pycom17g26790
rosa_chinensis RchiOBHm_Chr3g0491101
rosa_laevigata RLG00000022863
rosa_multiflora Rmu_sc0005038.1_g000022
rosa_roxburghii Rroxscaffold_6G00393200
rosa_rugosa Rorug03G0246100
rosa_samantha Rh3AG296300 Rh3BG332300 Rh3CG329600 Rh3DG330100
rosa_wichuraiana Rw3G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 21
AciI CCGC 1 cut(s) 254
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 1 cut(s) 128
AcvI CACGTG 1 cut(s) 250
AfaI GTAC 1 cut(s) 375
AfeI AGCGCT 1 cut(s) 68
AgsI TTSAA 4 cut(s) 104, 127, 133, 305
AjnI CCWGG 2 cut(s) 139, 401
AluBI AGCT 2 cut(s) 341, 433
AluI AGCT 2 cut(s) 341, 433
Alw21I GWGCWC 1 cut(s) 363
AlwI GGATC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 294
Aor51HI AGCGCT 1 cut(s) 68
AoxI GGCC 3 cut(s) 10, 222, 267
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 355
AvaI CYCGRG 1 cut(s) 294
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 24
BanI GGYRCC 1 cut(s) 21
BbrPI CACGTG 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 363
BccI CCATC 1 cut(s) 406
BceAI ACGGC 3 cut(s) 25, 99, 282
BciT130I CCWGG 2 cut(s) 141, 403
BcnI CCSGG 1 cut(s) 271
BfoI RGCGCY 1 cut(s) 70
Bme1390I CCNGG 3 cut(s) 141, 271, 403
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 3 cut(s) 141, 271, 403
BmsI GCATC 1 cut(s) 373
BpmI CTGGAG 2 cut(s) 36, 64
Bpu10I CCTNAGC 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 271
BsaAI YACGTR 1 cut(s) 250
BsaJI CCNNGG 1 cut(s) 402
BsaWI WCCGGW 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 53, 101
BseBI CCWGG 2 cut(s) 141, 403
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 398
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 2 cut(s) 53, 101
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 24
BseX3I CGGCCG 1 cut(s) 267
BsgI GTGCAG 1 cut(s) 188
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 12, 224, 269
BshNI GGYRCC 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 3 cut(s) 75, 270, 311
BslFI GGGAC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 3 cut(s) 12, 224, 269
BsoBI CYCGRG 1 cut(s) 294
Bsp1286I GDGCHC 2 cut(s) 24, 363
Bsp143I GATC 2 cut(s) 171, 328
BspACI CCGC 1 cut(s) 254
BspANI GGCC 3 cut(s) 12, 224, 269
BspCNI CTCAG 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 23
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 21
BsrI ACTGG 2 cut(s) 53, 101
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 2 cut(s) 171, 328
Bst2UI CCWGG 2 cut(s) 141, 403
Bst6I CTCTTC 1 cut(s) 150
BstBAI YACGTR 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 431
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 398
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 2 cut(s) 174, 331
BstMBI GATC 2 cut(s) 171, 328
BstMCI CGRYCG 1 cut(s) 270
BstNI CCWGG 2 cut(s) 141, 403
BstSCI CCNGG 3 cut(s) 139, 269, 401
BstSLI GKGCMC 1 cut(s) 24
BstZI CGGCCG 1 cut(s) 267
BsuRI GGCC 3 cut(s) 12, 224, 269
BtsCI GGATG 1 cut(s) 398
Cac8I GCNNGC 1 cut(s) 431
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 374
CviAII CATG 1 cut(s) 91
CviJI RGCY 7 cut(s) 5, 12, 100, 224, 269, 341, 433
CviKI_1 RGCY 7 cut(s) 5, 12, 100, 224, 269, 341, 433
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 1 cut(s) 342
DpnI GATC 2 cut(s) 173, 330
DpnII GATC 2 cut(s) 171, 328
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 269
EclXI CGGCCG 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 72
Eco47III AGCGCT 1 cut(s) 68
Eco52I CGGCCG 1 cut(s) 267
Eco72I CACGTG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 294
EcoRII CCWGG 2 cut(s) 139, 401
FaeI CATG 1 cut(s) 94
FaiI YATR 2 cut(s) 92, 122
FaqI GGGAC 1 cut(s) 277
FatI CATG 1 cut(s) 90
FokI GGATG 1 cut(s) 385
GlaI GCGC 1 cut(s) 68
GsuI CTGGAG 2 cut(s) 36, 64
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 3 cut(s) 12, 224, 269
HapII CCGG 3 cut(s) 75, 270, 311
HhaI GCGC 1 cut(s) 69
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 3 cut(s) 36, 46, 104
HpaII CCGG 3 cut(s) 75, 270, 311
HphI GGTGA 1 cut(s) 355
Hpy188I TCNGA 2 cut(s) 62, 333
Hpy188III TCNNGA 2 cut(s) 43, 296
HpyAV CCTTC 4 cut(s) 145, 173, 200, 316
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 2 cut(s) 169, 193
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 1 cut(s) 67
Kzo9I GATC 2 cut(s) 171, 328
LweI GCATC 1 cut(s) 373
MaeII ACGT 1 cut(s) 249
MalI GATC 2 cut(s) 173, 330
MboI GATC 2 cut(s) 171, 328
MboII GAAGA 2 cut(s) 167, 188
MhlI GDGCHC 2 cut(s) 24, 363
MluCI AATT 2 cut(s) 128, 300
MlyI GAGTC 1 cut(s) 55
MnlI CCTC 5 cut(s) 50, 60, 211, 214, 254
MseI TTAA 1 cut(s) 336
MspI CCGG 3 cut(s) 75, 270, 311
MspR9I CCNGG 3 cut(s) 141, 271, 403
MvaI CCWGG 2 cut(s) 141, 403
NciI CCSGG 1 cut(s) 271
NdeII GATC 2 cut(s) 171, 328
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 36, 104
PleI GAGTC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 250
Psp6I CCWGG 2 cut(s) 139, 401
PspCI CACGTG 1 cut(s) 250
PspGI CCWGG 2 cut(s) 139, 401
PspN4I GGNNCC 1 cut(s) 23
PspPI GGNCC 1 cut(s) 72
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
SaqAI TTAA 1 cut(s) 336
Sau3AI GATC 2 cut(s) 171, 328
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 3 cut(s) 141, 271, 403
SduI GDGCHC 2 cut(s) 24, 363
SetI ASST 8 cut(s) 145, 168, 211, 252, 327, 343, 348, 435
SfaNI GCATC 1 cut(s) 373
SinI GGWCC 1 cut(s) 72
Sse9I AATT 2 cut(s) 128, 300
SsiI CCGC 1 cut(s) 254
SspI AATATT 1 cut(s) 421
StyD4I CCNGG 3 cut(s) 139, 269, 401
TaiI ACGT 1 cut(s) 252
TaqI TCGA 1 cut(s) 174
TasI AATT 2 cut(s) 128, 300
TfiI GAWTC 2 cut(s) 36, 104
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.