pycom09g18210

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0014230
Physical Location & Seq
Forward (+)
18805177 .. 18806089
913 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g18210.1

Sequence Viewer

Length: 552 bp
ATGTCCAATACCAGTACTAACCCCATCAATATCATCAACGTCGAGCTTATAGTTGCTTCAAAATTGTTCACAGCAAACACAAAAAAGGAAGACCAGAGAAAAGAAACAAAAACAATGGCCGCTGATAGCATATTTGGGTACCCGTTCAGGCGCCTACTCTGGAGCCCTCCGGTTTTCCGTGAATGGTCAGGATCAACGGCCCTCATGGACTGGCTTGAATCCCCTAATGCCCACATATTCAAGATCAATGTTCCAGGTTTTAGAAAAGAGGACATAAAAGTGCAGATGGAAGAAGGGAACATTTTGCAGATAAAAGGGGAAGGCGGGAAAGAAGGAGAGGAGGCTCATGCAGCAAAAGACACTGTTTGGCACGTCGCGGAGAGGGGGACGGGAAAAGGAGACTTCTCTCGGGAAATCGAGTTGCCGGAAAATGTGAAAGTCGATCAGATAAAAGCTCAGGTTGAGAATGGTGTTCTCACGATTGTTGTCCCAAAAGATTCGACCCCCAAACCATCCAGAGTGCGAAACATCAATATCACTAGCAAGCTCTAA

Protein Analysis

184

Amino Acids

20.52

Weight (kDa)

7.92

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 70 - 180 6.5e-23 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37670
fragaria_vesca FvH4_1g07080
malus_domestica MD09G1271100.v1.1 MD17G1269200.v1.1
prunus_persica Prupe.3G017400_v2.0.a1
pyrus_communis pycom09g18210 pycom17g26790
rosa_chinensis RchiOBHm_Chr3g0491101
rosa_laevigata RLG00000022863
rosa_multiflora Rmu_sc0005038.1_g000022
rosa_roxburghii Rroxscaffold_6G00393200
rosa_rugosa Rorug03G0246100
rosa_samantha Rh3AG296300 Rh3BG332300 Rh3CG329600 Rh3DG330100
rosa_wichuraiana Rw3G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 138, 150
AccII CGCG 1 cut(s) 377
AciI CCGC 3 cut(s) 120, 324, 377
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 151
AfaI GTAC 2 cut(s) 16, 140
AgsI TTSAA 3 cut(s) 60, 218, 241
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 3 cut(s) 46, 455, 547
AluI AGCT 3 cut(s) 46, 455, 547
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 2 cut(s) 117, 198
ApeKI GCWGC 1 cut(s) 350
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 408
BanI GGYRCC 2 cut(s) 138, 150
BanII GRGCYC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 362
BccI CCATC 3 cut(s) 32, 280, 520
BceAI ACGGC 1 cut(s) 213
BciT130I CCWGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 2 cut(s) 120, 351
BlsI GCNGC 2 cut(s) 121, 352
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 408
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 3 cut(s) 140, 152, 164
BmrFI CCNGG 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 181
Bpu10I CCTNAGC 1 cut(s) 456
BsaHI GRCGYC 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 12, 215
BseBI CCWGG 1 cut(s) 255
BseGI GGATG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 2 cut(s) 12, 215
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 362
BsgI GTGCAG 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 377
BshFI GGCC 2 cut(s) 119, 200
BshNI GGYRCC 2 cut(s) 138, 150
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 2 cut(s) 170, 425
BslFI GGGAC 2 cut(s) 400, 473
BsmAI GTCTC 1 cut(s) 393
BsmFI GGGAC 2 cut(s) 400, 473
BsnI GGCC 2 cut(s) 119, 200
BsoBI CYCGRG 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 3 cut(s) 191, 243, 442
BspACI CCGC 3 cut(s) 120, 324, 377
BspANI GGCC 2 cut(s) 119, 200
BspCNI CTCAG 1 cut(s) 469
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 3 cut(s) 140, 152, 164
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 2 cut(s) 138, 150
BsrI ACTGG 2 cut(s) 12, 215
BssMI GATC 3 cut(s) 191, 243, 442
BssNI GRCGYC 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 364
BstACI GRCGYC 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 512
BstFNI CGCG 1 cut(s) 377
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 3 cut(s) 194, 246, 445
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 3 cut(s) 191, 243, 442
BstMWI GCNNNNNNNGC 1 cut(s) 350
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 1 cut(s) 362
BstV2I GAAGAC 1 cut(s) 96
BsuRI GGCC 2 cut(s) 119, 200
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 1 cut(s) 512
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 545
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 2 cut(s) 15, 139
CviAII CATG 2 cut(s) 205, 347
CviJI RGCY 8 cut(s) 46, 119, 165, 200, 214, 344, 455, 547
CviKI_1 RGCY 8 cut(s) 46, 119, 165, 200, 214, 344, 455, 547
CviQI GTAC 2 cut(s) 15, 139
DdeI CTNAG 1 cut(s) 456
DinI GGCGCC 1 cut(s) 152
DpnI GATC 3 cut(s) 193, 245, 444
DpnII GATC 3 cut(s) 191, 243, 442
EaeI YGGCCR 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 408
EcoRII CCWGG 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 167
EgeI GGCGCC 1 cut(s) 152
EheI GGCGCC 1 cut(s) 152
FaeI CATG 2 cut(s) 208, 350
FaiI YATR 6 cut(s) 50, 131, 206, 236, 275, 348
FaqI GGGAC 2 cut(s) 400, 473
FatI CATG 2 cut(s) 204, 346
FauI CCCGC 1 cut(s) 317
Fnu4HI GCNGC 2 cut(s) 120, 351
FokI GGATG 1 cut(s) 499
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 2 cut(s) 120, 351
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 152
GluI GCNGC 2 cut(s) 120, 351
GsuI CTGGAG 1 cut(s) 181
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 2 cut(s) 119, 200
HapII CCGG 2 cut(s) 170, 425
HhaI GCGC 1 cut(s) 153
Hin1I GRCGYC 1 cut(s) 151
Hin1II CATG 2 cut(s) 208, 350
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HinfI GANTC 2 cut(s) 218, 497
HpaII CCGG 2 cut(s) 170, 425
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 6 cut(s) 160, 189, 241, 410, 478, 516
Hpy8I GTNNAC 1 cut(s) 69
Hpy99I CGWCG 2 cut(s) 44, 377
HpyAV CCTTC 3 cut(s) 287, 314, 326
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 2 cut(s) 39, 372
HpyCH4V TGCA 3 cut(s) 283, 307, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 350
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 2 cut(s) 39, 372
Hsp92I GRCGYC 1 cut(s) 151
Hsp92II CATG 2 cut(s) 208, 350
HspAI GCGC 1 cut(s) 151
KasI GGCGCC 1 cut(s) 150
KpnI GGTACC 1 cut(s) 142
Kzo9I GATC 3 cut(s) 191, 243, 442
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 1 cut(s) 362
MaeI CTAG 1 cut(s) 540
MaeII ACGT 2 cut(s) 39, 372
MalI GATC 3 cut(s) 193, 245, 444
MboI GATC 3 cut(s) 191, 243, 442
MboII GAAGA 2 cut(s) 101, 302
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 1 cut(s) 62
Mly113I GGCGCC 1 cut(s) 151
MnlI CCTC 6 cut(s) 177, 212, 262, 331, 334, 375
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 2 cut(s) 170, 425
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 350
NarI GGCGCC 1 cut(s) 151
NdeII GATC 3 cut(s) 191, 243, 442
NlaIII CATG 2 cut(s) 208, 350
NlaIV GGNNCC 3 cut(s) 140, 152, 164
PfeI GAWTC 2 cut(s) 218, 497
PkrI GCNGC 2 cut(s) 121, 352
PluTI GGCGCC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 3 cut(s) 140, 152, 164
PspPI GGNCC 1 cut(s) 199
RsaI GTAC 2 cut(s) 16, 140
RsaNI GTAC 2 cut(s) 15, 139
RseI CAYNNNNRTG 1 cut(s) 278
SatI GCNGC 2 cut(s) 120, 351
Sau3AI GATC 3 cut(s) 191, 243, 442
Sau96I GGNCC 1 cut(s) 199
ScaI AGTACT 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 167
SetI ASST 7 cut(s) 42, 48, 259, 375, 457, 462, 549
SfoI GGCGCC 1 cut(s) 152
SmiMI CAYNNNNRTG 1 cut(s) 278
Sse9I AATT 1 cut(s) 62
SsiI CCGC 3 cut(s) 120, 324, 377
SspDI GGCGCC 1 cut(s) 150
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 2 cut(s) 42, 375
TaqI TCGA 4 cut(s) 42, 417, 441, 500
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 14
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 218, 497
TscAI CASTG 1 cut(s) 367
TseI GCWGC 1 cut(s) 350
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 1 cut(s) 367
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.