MD17G1269200.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014230
Physical Location & Seq
Forward (+)
32927931 .. 32928852
922 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1269200.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGACTGGTTTGAATCCCCTAATTCCCCCATAATCAAGGTCAATGTTCCAGGTTTTAGAAAAGAGGACATAAAAGGGCAGATAGAAGAAGGGAATATTTTGCAGATGAAAGGGGAAGGCGGGAAAGAAAGAGAGGAGGCTCATGCAGCAAAATACACTGTTTGGAACGTCGCGGAGAGGGGGACGGGAAAAGGAGACTTTTCCCGGGAAATCGCATTGCCGGAAAATGTGAAAGTCGATCAGATAAAAGCTCAGGTTGAGAATGGTGTTCTCACCATTGTTGTCCCAAAAGATACGACCCCCAAAACCATCCAAAGTGCGAAACATCAATATCACTAG

Protein Analysis

113

Amino Acids

12.53

Weight (kDa)

6.3

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 3 - 103 2.1e-17 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37670
fragaria_vesca FvH4_1g07080
malus_domestica MD09G1271100.v1.1 MD17G1269200.v1.1
prunus_persica Prupe.3G017400_v2.0.a1
pyrus_communis pycom09g18210 pycom17g26790
rosa_chinensis RchiOBHm_Chr3g0491101
rosa_laevigata RLG00000022863
rosa_multiflora Rmu_sc0005038.1_g000022
rosa_roxburghii Rroxscaffold_6G00393200
rosa_rugosa Rorug03G0246100
rosa_samantha Rh3AG296300 Rh3BG332300 Rh3CG329600 Rh3DG330100
rosa_wichuraiana Rw3G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 173
AciI CCGC 2 cut(s) 120, 173
AgsI TTSAA 1 cut(s) 14
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 146
AsuC2I CCSGG 2 cut(s) 205, 206
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 204
BbvI GCAGC 1 cut(s) 158
BccI CCATC 1 cut(s) 317
BciT130I CCWGG 1 cut(s) 51
BcnI CCSGG 2 cut(s) 205, 206
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 1 cut(s) 337
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 3 cut(s) 51, 205, 206
BmeT110I CYCGRG 1 cut(s) 204
BmrFI CCNGG 3 cut(s) 51, 205, 206
Bpu10I CCTNAGC 1 cut(s) 252
BpuMI CCSGG 2 cut(s) 205, 206
BsaJI CCNNGG 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 215
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 1 cut(s) 309
BseMI GCAATG 1 cut(s) 215
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 158
Bsh1236I CGCG 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 205, 221
BslFI GGGAC 2 cut(s) 196, 269
BsmAI GTCTC 1 cut(s) 189
BsmFI GGGAC 2 cut(s) 196, 269
BsoBI CYCGRG 1 cut(s) 204
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 2 cut(s) 120, 173
BspCNI CTCAG 1 cut(s) 265
BspFNI CGCG 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 215
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 309
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 3 cut(s) 49, 203, 204
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 1 cut(s) 158
BtsCI GGATG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 156
Cfr9I CCCGGG 1 cut(s) 204
CviAII CATG 1 cut(s) 143
CviJI RGCY 2 cut(s) 140, 251
CviKI_1 RGCY 2 cut(s) 140, 251
DdeI CTNAG 1 cut(s) 252
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 49
FaeI CATG 1 cut(s) 146
FaiI YATR 3 cut(s) 32, 71, 144
FaqI GGGAC 2 cut(s) 196, 269
FatI CATG 1 cut(s) 142
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 1 cut(s) 337
GluI GCNGC 1 cut(s) 147
HapII CCGG 2 cut(s) 205, 221
Hin1II CATG 1 cut(s) 146
HinfI GANTC 1 cut(s) 14
HpaII CCGG 2 cut(s) 205, 221
HphI GGTGA 1 cut(s) 265
Hpy188I TCNGA 1 cut(s) 243
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 2 cut(s) 83, 110
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 2 cut(s) 103, 146
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 1 cut(s) 252
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 1 cut(s) 146
Kzo9I GATC 1 cut(s) 238
LpnPI CCDG 5 cut(s) 36, 63, 218, 234, 239
Lsp1109I GCAGC 1 cut(s) 158
MaeI CTAG 1 cut(s) 337
MaeII ACGT 1 cut(s) 168
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 1 cut(s) 98
MluCI AATT 1 cut(s) 22
MnlI CCTC 4 cut(s) 58, 127, 130, 171
MspI CCGG 2 cut(s) 205, 221
MspR9I CCNGG 3 cut(s) 51, 205, 206
MvaI CCWGG 1 cut(s) 51
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 146
NciI CCSGG 2 cut(s) 205, 206
NdeII GATC 1 cut(s) 238
NlaIII CATG 1 cut(s) 146
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 1 cut(s) 238
ScrFI CCNGG 3 cut(s) 51, 205, 206
SetI ASST 5 cut(s) 42, 55, 171, 253, 258
SmaI CCCGGG 1 cut(s) 206
Sse9I AATT 1 cut(s) 22
SsiI CCGC 2 cut(s) 120, 173
SspI AATATT 1 cut(s) 97
SspMI CTAG 1 cut(s) 337
StyD4I CCNGG 3 cut(s) 49, 203, 204
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 1 cut(s) 171
TaqI TCGA 1 cut(s) 237
TasI AATT 1 cut(s) 22
TfiI GAWTC 1 cut(s) 14
TscAI CASTG 1 cut(s) 163
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 122
TspMI CCCGGG 1 cut(s) 204
TspRI CASTG 1 cut(s) 163
XmaI CCCGGG 1 cut(s) 204
XspI CTAG 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.