FvH4_1g10150

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005266
Physical Location & Seq
Reverse (-)
5513097 .. 5520052
6956 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10150.t2

Sequence Viewer

Length: 1806 bp
ATGTTTGGTTATGTGGTTCGGGCTGTTGGGTGTGGCTATGGCGGCAGAGGAGACTACTACGACGACTACGGCGTCGAGGGAGCTGGACCGGACGCCGACGTGGGCCGTTGCCGGAGTTTGTGCTGTTATCATCATCATTTCTTTGGTTTTGGAGAAAGTCCTGCACACAGTCGGAACGCAAGCTACTTTTCCTTGGTGATTGGGAGACTCTGTTTCTGCTTTGATCTGGTTGACTTGTGGCTTTCAGAGAGGCACAAGAGAGGTCTGTTTGAAGCTCTGGAGAAGGTTAAAGCGGAGCTGATGATTTTGGGTTTTATTTCACTAATCCTGACCTTCAGTCAAAGTTACATAGCCAAAATCTGTATTCCGGTTAAGGTTGCAAACACTATGCTGCCATGTAGTTTAGCTTCAACTGAGAAGGAGGAAGAAACAACAAGTCGCCGAAGACTCTTATCAACTGACCGCCGGTTTTTAGCTGAAGCAACTACTACCACATGCAAGAGCGGTTATGAGCCACTTATCTCCATTAATGGATTGCACCAATTACACATCCTCATATTCTTCTTGGCTGCCTTTCACGTAGCATACAGTGCTATCACAATGCTTCTTGGGAGGCTAAAGATTCGAGGATGGAAGGCATGGGAGGCTGAGACTTCATCCCATAGCTATGAGTTCTCAAATGATCCTTCAAGATTCAGACTTACTCATGAAACATCATTTGTGAAAGCACATACCAGTTACTGGACTAGGATTCCAGTCTTCTTTTATGTTGGATGCTTCTTTCGACAATTTTTTAGGTCTGTTAGTAAATCTGACTACTTGACAGTGCGCAATGGATTCATTTCTGTCCATTTAGCCCCTGGAAGTAAATTTAACTTCCAAAAGTATATCAAGAGATCGTTAGAGGATGACTTTCAGGTTGTTGTCGGAGTCAGTCCCGTACTATGGGCATCATTTGTGGTATTTTTGCTTCTTAATGTTAGCGGATGGCAAGCACTATTTTGGGCATCCACGATCCCTGTCATTATAATCTTAGCCATTGGAACAGAACTTCAATCCGTTCTGACCAAGATGGCTCTGGAAATCTCAGAAAGGCATGCAGTGGTTCAAGGGATTCCTCTTGTACAAGGCTCTGATAGATACTTTTGGTTTGGTCGACCGCACTTGGTTCTTCATCTCATCCACTTTGCGTTGTTTCAGAATGCGTTCCAAATTATATATTTCTTATGGATATGGTATTCATTTGGGTTGCAATCTTGCTTCCATGCCAATTTCAAGCTTGCAATCGCAAAAGTGATTGTTGGGGGTGGAGTTCTATGTGTCTGCAGCTACATCACATTGCCACTATATGCCCTTGTAACTCAGATGGGTTCAAACATGAAAAAGTCCATCTTTGACGAACAAACATCCAAGGCCCTTAAGAAGTGGCACAATGCTGTGAAAAAGAAGGGGCAAGGAGGCAAGTCCCCTGCTCGAACCTTGGGTGGCAGCACTGTTGGTGGCAGCACCGTTGGTGGAAGCACTATTGGTGGAAGCTCATCCGTTTCAGCCGTGCACACCTCTGGACCTACATTGCACCGTTTCAAAACAACCGGTCACTCAACCCGCTCATCCGTTTATGAAGATCATGAGGCATCTGATCATGAAACTGATCCTTTGTCACCAGGATCATCAACAAATTTTATAGTAGAAGTGGATAAAGCTGAGCAACAAACTGAAATAACCGAGCCACACCATGAGGAGCAAACCACCATCGAAATGGACGAGTTCACGTTCACAAAGCCTGATCTAGCAAAACAAACATGA

Protein Analysis

602

Amino Acids

67.28

Weight (kDa)

8.54

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 33 - 489 4e-211 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1028
AasI GACNNNNNNGTC 1 cut(s) 71
Acc16I TGCGCA 1 cut(s) 830
AccB7I CCANNNNNTGG 1 cut(s) 741
AccBSI CCGCTC 2 cut(s) 504, 1608
AccI GTMKAC 1 cut(s) 1156
AciI CCGC 7 cut(s) 42, 293, 463, 504, 984, 1160, 1606
AclWI GGATC 4 cut(s) 677, 1009, 1646, 1675
AcsI RAATTY 2 cut(s) 869, 1678
AcuI CTGAAG 2 cut(s) 319, 498
AcyI GRCGYC 2 cut(s) 72, 93
AfaI GTAC 2 cut(s) 942, 1125
AfiI CCNNNNNNNGG 2 cut(s) 741, 945
AflII CTTAAG 1 cut(s) 1418
AgeI ACCGGT 1 cut(s) 1592
AgsI TTSAA 8 cut(s) 272, 411, 690, 1055, 1109, 1276, 1374, 1585
AhdI GACNNNNNGTC 1 cut(s) 336
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 2 cut(s) 859, 1663
Alw21I GWGCWC 1 cut(s) 1557
Alw26I GTCTC 3 cut(s) 45, 199, 644
Alw44I GTGCAC 1 cut(s) 1553
AlwI GGATC 4 cut(s) 677, 1009, 1646, 1675
AlwNI CAGNNNCTG 1 cut(s) 741
AoxI GGCC 2 cut(s) 103, 1413
ApaLI GTGCAC 1 cut(s) 1553
ApeKI GCWGC 5 cut(s) 391, 569, 1326, 1488, 1503
ApoI RAATTY 2 cut(s) 869, 1678
AseI ATTAAT 1 cut(s) 528
AsiGI ACCGGT 1 cut(s) 1592
Asp700I GAANNNNTTC 1 cut(s) 1205
AspLEI GCGC 1 cut(s) 831
AspS9I GGNCC 4 cut(s) 86, 103, 1414, 1565
AsuHPI GGTGA 2 cut(s) 208, 1653
AvaII GGWCC 2 cut(s) 86, 1565
BaeGI GKGCMC 1 cut(s) 1557
BarI GAAGNNNNNNTAC 4 cut(s) 391, 423, 954, 986
BbsI GAAGAC 2 cut(s) 451, 751
Bbv12I GWGCWC 1 cut(s) 1557
BbvI GCAGC 5 cut(s) 378, 556, 1338, 1500, 1515
BccI CCATC 6 cut(s) 624, 981, 1066, 1360, 1397, 1760
BceAI ACGGC 3 cut(s) 85, 90, 1535
BciT130I CCWGG 2 cut(s) 861, 1665
BclI TGATCA 1 cut(s) 1639
BcoDI GTCTC 3 cut(s) 45, 199, 644
BfaI CTAG 2 cut(s) 747, 1790
BfmI CTRYAG 1 cut(s) 1324
BfrI CTTAAG 1 cut(s) 1418
BisI GCNGC 6 cut(s) 43, 392, 570, 1327, 1489, 1504
BlpI GCTNAGC 1 cut(s) 1704
BlsI GCNGC 6 cut(s) 44, 393, 571, 1328, 1490, 1505
Bme1390I CCNGG 2 cut(s) 861, 1665
Bme18I GGWCC 2 cut(s) 86, 1565
BmeRI GACNNNNNGTC 1 cut(s) 336
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 4 cut(s) 86, 103, 1414, 1565
BmrFI CCNGG 2 cut(s) 861, 1665
BmsI GCATC 4 cut(s) 764, 959, 1016, 1643
BpiI GAAGAC 2 cut(s) 451, 751
BpmI CTGGAG 1 cut(s) 299
Bpu1102I GCTNAGC 1 cut(s) 1704
BsaAI YACGTR 1 cut(s) 580
BsaHI GRCGYC 2 cut(s) 72, 93
BsaJI CCNNGG 4 cut(s) 192, 859, 1410, 1479
BsaWI WCCGGW 3 cut(s) 88, 367, 1592
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 2 cut(s) 741, 945
Bse118I RCCGGY 2 cut(s) 465, 1592
Bse1I ACTGG 3 cut(s) 735, 746, 755
Bse3DI GCAATG 3 cut(s) 838, 1337, 1571
BseBI CCWGG 2 cut(s) 861, 1665
BseDI CCNNGG 4 cut(s) 192, 859, 1410, 1479
BseLI CCNNNNNNNGG 2 cut(s) 741, 945
BseMI GCAATG 3 cut(s) 838, 1337, 1571
BseMII CTCAG 5 cut(s) 405, 639, 1101, 1376, 1695
BseNI ACTGG 3 cut(s) 735, 746, 755
BseRI GAGGAG 2 cut(s) 63, 1754
BseSI GKGCMC 1 cut(s) 1557
BseXI GCAGC 5 cut(s) 378, 556, 1338, 1500, 1515
BsgI GTGCAG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 1160
BshFI GGCC 2 cut(s) 105, 1415
BshTI ACCGGT 1 cut(s) 1592
BsiEI CGRYCG 1 cut(s) 1160
BsiHKAI GWGCWC 1 cut(s) 1557
BsiSI CCGG 5 cut(s) 89, 112, 368, 466, 1593
BslFI GGGAC 2 cut(s) 921, 1450
BslI CCNNNNNNNGG 2 cut(s) 741, 945
BsmAI GTCTC 3 cut(s) 45, 199, 644
BsmFI GGGAC 2 cut(s) 921, 1450
BsmI GAATGC 1 cut(s) 1207
BsnI GGCC 2 cut(s) 105, 1415
Bsp1286I GDGCHC 1 cut(s) 1557
Bsp1407I TGTACA 1 cut(s) 1123
Bsp143I GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
Bsp1720I GCTNAGC 1 cut(s) 1704
BspACI CCGC 7 cut(s) 42, 293, 463, 504, 984, 1160, 1606
BspANI GGCC 2 cut(s) 105, 1415
BspCNI CTCAG 5 cut(s) 406, 640, 1100, 1375, 1696
BspHI TCATGA 3 cut(s) 706, 1627, 1642
BspMAI CTGCAG 1 cut(s) 1328
BspPI GGATC 4 cut(s) 677, 1009, 1646, 1675
BspTI CTTAAG 1 cut(s) 1418
BsrBI CCGCTC 2 cut(s) 504, 1608
BsrDI GCAATG 3 cut(s) 838, 1337, 1571
BsrFI RCCGGY 2 cut(s) 465, 1592
BsrGI TGTACA 1 cut(s) 1123
BsrI ACTGG 3 cut(s) 735, 746, 755
BssAI RCCGGY 2 cut(s) 465, 1592
BssECI CCNNGG 4 cut(s) 192, 859, 1410, 1479
BssMI GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
BssNI GRCGYC 2 cut(s) 72, 93
BssT1I CCWWGG 3 cut(s) 192, 1410, 1479
Bst2UI CCWGG 2 cut(s) 861, 1665
Bst4CI ACNGT 6 cut(s) 170, 590, 826, 1495, 1510, 1580
BstACI GRCGYC 2 cut(s) 72, 93
BstAFI CTTAAG 1 cut(s) 1418
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 1123
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 4 cut(s) 181, 993, 1098, 1281
BstDEI CTNAG 6 cut(s) 414, 648, 1033, 1087, 1362, 1704
BstHHI GCGC 1 cut(s) 831
BstKTI GATC 9 cut(s) 226, 685, 899, 1017, 1627, 1642, 1654, 1670, 1789
BstMAI GTCTC 3 cut(s) 45, 199, 644
BstMBI GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
BstMCI CGRYCG 1 cut(s) 1160
BstMWI GCNNNNNNNGC 3 cut(s) 42, 590, 644
BstNI CCWGG 2 cut(s) 861, 1665
BstNSI RCATGY 2 cut(s) 498, 1100
BstSCI CCNGG 2 cut(s) 859, 1663
BstSFI CTRYAG 1 cut(s) 1324
BstSLI GKGCMC 1 cut(s) 1557
BstV1I GCAGC 5 cut(s) 378, 556, 1338, 1500, 1515
BstV2I GAAGAC 2 cut(s) 451, 751
BstXI CCANNNNNNTGG 1 cut(s) 1759
BsuRI GGCC 2 cut(s) 105, 1415
BtrI CACGTC 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 4 cut(s) 595, 831, 1107, 1491
Cac8I GCNNGC 4 cut(s) 181, 993, 1098, 1281
CaiI CAGNNNCTG 1 cut(s) 741
CciI TCATGA 3 cut(s) 706, 1627, 1642
CfoI GCGC 1 cut(s) 831
Cfr10I RCCGGY 2 cut(s) 465, 1592
Cfr13I GGNCC 4 cut(s) 86, 103, 1414, 1565
CseI GACGC 2 cut(s) 61, 101
Csp6I GTAC 2 cut(s) 941, 1124
CspAI ACCGGT 1 cut(s) 1592
CviQI GTAC 2 cut(s) 941, 1124
DdeI CTNAG 6 cut(s) 414, 648, 1033, 1087, 1362, 1704
DpnI GATC 9 cut(s) 225, 684, 898, 1016, 1626, 1641, 1653, 1669, 1788
DpnII GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
DrdI GACNNNNNNGTC 1 cut(s) 71
DriI GACNNNNNGTC 1 cut(s) 336
DseDI GACNNNNNNGTC 1 cut(s) 71
Eam1105I GACNNNNNGTC 1 cut(s) 336
Eco130I CCWWGG 3 cut(s) 192, 1410, 1479
Eco47I GGWCC 2 cut(s) 86, 1565
Eco57I CTGAAG 2 cut(s) 319, 498
EcoO109I RGGNCCY 1 cut(s) 1414
EcoRII CCWGG 2 cut(s) 859, 1663
EcoT14I CCWWGG 3 cut(s) 192, 1410, 1479
ErhI CCWWGG 3 cut(s) 192, 1410, 1479
FalI AAGNNNNNCTT 2 cut(s) 1376, 1408
FaqI GGGAC 2 cut(s) 921, 1450
FauI CCCGC 1 cut(s) 1613
FbaI TGATCA 1 cut(s) 1639
FblI GTMKAC 1 cut(s) 1156
Fnu4HI GCNGC 6 cut(s) 43, 392, 570, 1327, 1489, 1504
Fsp4HI GCNGC 6 cut(s) 43, 392, 570, 1327, 1489, 1504
FspBI CTAG 2 cut(s) 747, 1790
FspI TGCGCA 1 cut(s) 830
GlaI GCGC 1 cut(s) 830
GluI GCNGC 6 cut(s) 43, 392, 570, 1327, 1489, 1504
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 2 cut(s) 105, 1415
HapII CCGG 5 cut(s) 89, 112, 368, 466, 1593
HgaI GACGC 2 cut(s) 61, 101
HhaI GCGC 1 cut(s) 831
Hin1I GRCGYC 2 cut(s) 72, 93
Hin6I GCGC 1 cut(s) 829
HinP1I GCGC 1 cut(s) 829
HincII GTYRAC 2 cut(s) 232, 1157
HindII GTYRAC 2 cut(s) 232, 1157
HindIII AAGCTT 1 cut(s) 1277
HinfI GANTC 8 cut(s) 207, 447, 622, 693, 751, 837, 930, 1114
HpaII CCGG 5 cut(s) 89, 112, 368, 466, 1593
HphI GGTGA 2 cut(s) 208, 1653
Hpy166II GTNNAC 5 cut(s) 232, 1157, 1555, 1770, 1776
Hpy188III TCNNGA 9 cut(s) 278, 328, 690, 707, 892, 1079, 1563, 1628, 1643
Hpy8I GTNNAC 5 cut(s) 232, 1157, 1555, 1770, 1776
Hpy99I CGWCG 3 cut(s) 65, 77, 101
HpyAV CCTTC 6 cut(s) 277, 343, 412, 628, 696, 1441
HpyCH4III ACNGT 6 cut(s) 170, 590, 826, 1495, 1510, 1580
HpyCH4IV ACGT 3 cut(s) 99, 579, 1772
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 590, 644
HpyF3I CTNAG 6 cut(s) 414, 648, 1033, 1087, 1362, 1704
HpySE526I ACGT 3 cut(s) 99, 579, 1772
Hsp92I GRCGYC 2 cut(s) 72, 93
HspAI GCGC 1 cut(s) 829
Ksp22I TGATCA 1 cut(s) 1639
Kzo9I GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
LmnI GCTCC 3 cut(s) 80, 295, 1741
Lsp1109I GCAGC 5 cut(s) 378, 556, 1338, 1500, 1515
LweI GCATC 4 cut(s) 764, 959, 1016, 1643
MaeI CTAG 2 cut(s) 747, 1790
MaeII ACGT 3 cut(s) 99, 579, 1772
MaeIII GTNAC 5 cut(s) 344, 737, 1357, 1595, 1659
MalI GATC 9 cut(s) 225, 684, 898, 1016, 1626, 1641, 1653, 1669, 1788
MbiI CCGCTC 2 cut(s) 504, 1608
MboI GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
MboII GAAGA 6 cut(s) 437, 456, 553, 751, 1163, 1634
MhlI GDGCHC 1 cut(s) 1557
MluCI AATT 6 cut(s) 542, 788, 869, 1212, 1270, 1678
MlyI GAGTC 3 cut(s) 201, 441, 939
MmeI TCCRAC 3 cut(s) 152, 751, 907
MroXI GAANNNNTTC 1 cut(s) 1205
MseI TTAA 6 cut(s) 288, 372, 528, 873, 975, 1419
MslI CAYNNNNRTG 2 cut(s) 666, 1757
MspCI CTTAAG 1 cut(s) 1418
MspI CCGG 5 cut(s) 89, 112, 368, 466, 1593
MspR9I CCNGG 2 cut(s) 861, 1665
Mva1269I GAATGC 1 cut(s) 1207
MvaI CCWGG 2 cut(s) 861, 1665
MwoI GCNNNNNNNGC 3 cut(s) 42, 590, 644
NdeII GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
NmuCI GTSAC 2 cut(s) 1595, 1659
NsbI TGCGCA 1 cut(s) 830
NspI RCATGY 2 cut(s) 498, 1100
PaeI GCATGC 1 cut(s) 1100
PagI TCATGA 3 cut(s) 706, 1627, 1642
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 1761
PctI GAATGC 1 cut(s) 1207
PdmI GAANNNNTTC 1 cut(s) 1205
PfeI GAWTC 5 cut(s) 622, 693, 751, 837, 1114
PflMI CCANNNNNTGG 1 cut(s) 741
PinAI ACCGGT 1 cut(s) 1592
PkrI GCNGC 6 cut(s) 44, 393, 571, 1328, 1490, 1505
PleI GAGTC 3 cut(s) 201, 441, 938
PpsI GAGTC 3 cut(s) 201, 441, 938
Ppu21I YACGTR 1 cut(s) 580
PshBI ATTAAT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 1028
Psp6I CCWGG 2 cut(s) 859, 1663
PspGI CCWGG 2 cut(s) 859, 1663
PspPI GGNCC 4 cut(s) 86, 103, 1414, 1565
PsrI GAACNNNNNNTAC 2 cut(s) 167, 199
PstI CTGCAG 1 cut(s) 1328
PstNI CAGNNNCTG 1 cut(s) 741
RsaI GTAC 2 cut(s) 942, 1125
RsaNI GTAC 2 cut(s) 941, 1124
RseI CAYNNNNRTG 2 cut(s) 666, 1757
SalI GTCGAC 1 cut(s) 1155
SaqAI TTAA 6 cut(s) 288, 372, 528, 873, 975, 1419
SatI GCNGC 6 cut(s) 43, 392, 570, 1327, 1489, 1504
Sau3AI GATC 9 cut(s) 223, 682, 896, 1014, 1624, 1639, 1651, 1667, 1786
Sau96I GGNCC 4 cut(s) 86, 103, 1414, 1565
SchI GAGTC 3 cut(s) 201, 441, 939
ScrFI CCNGG 2 cut(s) 861, 1665
SduI GDGCHC 1 cut(s) 1557
SfaNI GCATC 4 cut(s) 764, 959, 1016, 1643
SfcI CTRYAG 1 cut(s) 1324
SinI GGWCC 2 cut(s) 86, 1565
SmiMI CAYNNNNRTG 2 cut(s) 666, 1757
SmlI CTYRAG 1 cut(s) 1418
SmoI CTYRAG 1 cut(s) 1418
SphI GCATGC 1 cut(s) 1100
Sse9I AATT 6 cut(s) 542, 788, 869, 1212, 1270, 1678
SsiI CCGC 7 cut(s) 42, 293, 463, 504, 984, 1160, 1606
SspMI CTAG 2 cut(s) 747, 1790
StyD4I CCNGG 2 cut(s) 859, 1663
StyI CCWWGG 3 cut(s) 192, 1410, 1479
TaaI ACNGT 6 cut(s) 170, 590, 826, 1495, 1510, 1580
TaiI ACGT 3 cut(s) 102, 582, 1775
TaqI TCGA 6 cut(s) 75, 625, 784, 1156, 1474, 1755
TasI AATT 6 cut(s) 542, 788, 869, 1212, 1270, 1678
TatI WGTACW 1 cut(s) 1123
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 5 cut(s) 622, 693, 751, 837, 1114
Tru1I TTAA 6 cut(s) 288, 372, 528, 873, 975, 1419
Tru9I TTAA 6 cut(s) 288, 372, 528, 873, 975, 1419
TscAI CASTG 4 cut(s) 595, 831, 1107, 1498
TseFI GTSAC 2 cut(s) 1595, 1659
TseI GCWGC 5 cut(s) 391, 569, 1326, 1488, 1503
Tsp45I GTSAC 2 cut(s) 1595, 1659
TspDTI ATGAA 8 cut(s) 645, 723, 829, 1163, 1230, 1394, 1635, 1659
TspGWI ACGGA 3 cut(s) 1048, 1531, 1603
TspRI CASTG 4 cut(s) 595, 831, 1107, 1498
Van91I CCANNNNNTGG 1 cut(s) 741
Vha464I CTTAAG 1 cut(s) 1418
VneI GTGCAC 1 cut(s) 1553
VpaK11BI GGWCC 2 cut(s) 86, 1565
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 2 cut(s) 869, 1678
XceI RCATGY 2 cut(s) 498, 1100
XcmI CCANNNNNNNNNTGG 3 cut(s) 857, 1075, 1756
XmiI GTMKAC 1 cut(s) 1156
XmnI GAANNNNTTC 1 cut(s) 1205
XspI CTAG 2 cut(s) 747, 1790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.