MD02G1113500.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005266
Physical Location & Seq
Reverse (-)
9216561 .. 9221626
5066 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1113500.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGGTGCCTTTCTCTCTGCTGGGTGTGAGCTTATGCCTTGTTGCTGGGTTGCTGGATACAGCCATGGGAGCAGGAAAGACCACCTCGTACTCGAGGGAGCTCGACCGTACACCCACCTGGGCTGTTGCTGGTGTCTGTGCTGTTATCATCATCATCTCATTGGTTTTGGAGAAGGTCCTGCACAAAGTTGGAATATGGCTTACAGATAGGCACAAGAGAGCGCTGTTTGAATCTCTTGAGAAGGTTAAAGCAGAGTTGATGATTCTAGGTTTTATTTCGCTGATCCTGACTTTCGGGCAGAGTTACATTGCCAAAATATGTATTCCACTCAATGTTGCAGACACTATGCTGCCATGTAGAGTAGATGATAAGGAGGAGGAGGAGGAGTCAACTAGTCACCGGAGACTCTTATGGTACGAACGCAGATCTTTAGCTGCTGCCTCTGATTATAAATGCAAGACTGGATACGAGCCACTTATAACAGTTAATGGATTGCACCAACTGCACATCCTCATATTCTTCTTAGCCGCCTTTCACGTGCTATACAGTCTTATCACGATGCTGCTCGGGAGGCTAAAGATTCGAGGCTGGAAGCATTGGGAGGCAGAGACTTCAAGTGATGACTATGAGTTTTCCAACGATCCTTCGAGATTCAGACTTACTCACCAGATATCATTTGTTAGAGCACACACCAGTTTCTGGACTAAGATTCCTTTCTTCTTTTATTTCGGATGCTTCTTTCGACAATTTTTTAGGTCTGTTAGTAAGGCTGACTACTTAACACTGCGCAATGGATTCATCACTGTCCATTTAGGTGCAGGAAGTAAATTTAACTTCCAAAAATATATCAAGAGATCATTAGAGGATGACTTCAAGGTCGTCGTTGGGGTTAGTCCTCTATTGTGGGCATCATTTGTGATCTTTTTGCTCCTTAATGTTAAAGGATGGCAGGCACTCTTTTGGGCATCCTTGATCCCTCTCGTTATAATTTTACTCGTTGGAACAAAACTTCAGGCCATCCTGACTAAGATGGCCATTGAAATTGCAGAAAGGCATGCTGTGGTTCAAGGGATTCCTCTAGTACAAGGATCTGACAAATATTTTTGGTTTGGTCGGCCTCAATTGATTCTTAATCTTATCCATTTCGCGCTGTTTCAGAACGCATTCCAAATTATATACTTTTTCTGGATATGGTATTCATTTGGGTTGAAATCTTGCTTCCATGCCAATTTCGAGCTCGCAATCGCAAAAGTAATTCTCGGGGTTGGTGTTCTATGTTTGTGCAGCTACATTACACTTCCTCTGTATGCCCTTGTAACTCAGATGGGTTCACACATGAAAAGGTCCATCTTTGATGAACAAACATCCAAGGCCCTTAAGAAGTGGCACATGGCGGTGAAAAAGAAGACGCACGGAGGCAAGTCCCCTACCCGAACCCTAGGTGGTGAAAGCTCCACCATTTCAACGATGCGCTCCTCTACCTCAGGACACACGCTGCATCGCTTCAAAACAACTGGTCACTCAACTCGCTCAGCCACCTTTGAGGACCATGAGACATCTGATCCCGAAACTGATCCTCAGTCACCTTCATCAACAACACACTTGATTGTAAGAGTGGATCAAATTGAGCAACAAACCGAGCTAAATGAACCCCACGACGGGGAACAGACCAATATTCCAGATGATTTCTCGTTCATCCCACCTGCCCCGGGTAAAGAAACATGA

Protein Analysis

575

Amino Acids

65.14

Weight (kDa)

8.93

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 32 - 500 9.7e-214 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 450, 479, 986
AarI CACCTGC 1 cut(s) 1711
AasI GACNNNNNNGTC 1 cut(s) 875
Acc16I TGCGCA 1 cut(s) 788
Acc36I ACCTGC 1 cut(s) 1711
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 699
AccII CGCG 1 cut(s) 1148
AciI CCGC 2 cut(s) 528, 1394
AclWI GGATC 7 cut(s) 277, 635, 967, 1096, 1556, 1568, 1626
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 1 cut(s) 995
AcvI CACGTG 1 cut(s) 538
AfaI GTAC 4 cut(s) 89, 109, 416, 1083
AfeI AGCGCT 1 cut(s) 222
AfiI CCNNNNNNNGG 5 cut(s) 699, 1658, 1659, 1660, 1709
AflII CTTAAG 1 cut(s) 1376
AgsI TTSAA 8 cut(s) 230, 615, 874, 1040, 1067, 1210, 1464, 1507
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 7 cut(s) 30, 100, 434, 1237, 1287, 1452, 1642
AluI AGCT 7 cut(s) 30, 100, 434, 1237, 1287, 1452, 1642
Alw21I GWGCWC 3 cut(s) 102, 688, 1239
Alw26I GTCTC 3 cut(s) 397, 602, 1547
AlwI GGATC 7 cut(s) 277, 635, 967, 1096, 1556, 1568, 1626
AlwNI CAGNNNCTG 1 cut(s) 699
Ama87I CYCGRG 4 cut(s) 91, 566, 1259, 1708
Aor51HI AGCGCT 1 cut(s) 222
AoxI GGCC 4 cut(s) 1014, 1032, 1115, 1371
ApeKI GCWGC 6 cut(s) 349, 434, 437, 562, 1284, 1495
ApoI RAATTY 1 cut(s) 827
Asp700I GAANNNNTTC 1 cut(s) 1163
AspA2I CCTAGG 1 cut(s) 1438
AspLEI GCGC 4 cut(s) 223, 789, 1150, 1473
AspS9I GGNCC 4 cut(s) 175, 1344, 1372, 1546
AsuC2I CCSGG 2 cut(s) 1709, 1710
AsuHPI GGTGA 5 cut(s) 389, 656, 1408, 1457, 1575
AvaI CYCGRG 4 cut(s) 91, 566, 1259, 1708
AvaII GGWCC 3 cut(s) 175, 1344, 1546
AvrII CCTAGG 1 cut(s) 1438
AxyI CCTNAGG 1 cut(s) 1483
BalI TGGCCA 1 cut(s) 1034
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 102, 1239
BbrPI CACGTG 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 1412
Bbv12I GWGCWC 3 cut(s) 102, 688, 1239
BbvI GCAGC 6 cut(s) 336, 421, 424, 549, 1296, 1482
BccI CCATC 5 cut(s) 939, 1024, 1025, 1318, 1355
BciT130I CCWGG 1 cut(s) 118
BciVI GTATCC 2 cut(s) 49, 458
BcnI CCSGG 2 cut(s) 1709, 1710
BcoDI GTCTC 3 cut(s) 397, 602, 1547
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 4 cut(s) 266, 393, 1079, 1439
BfoI RGCGCY 1 cut(s) 224
BfrI CTTAAG 1 cut(s) 1376
BfuAI ACCTGC 1 cut(s) 1711
BfuI GTATCC 2 cut(s) 49, 458
BglII AGATCT 1 cut(s) 425
BisI GCNGC 7 cut(s) 350, 435, 438, 528, 563, 1285, 1496
BlnI CCTAGG 1 cut(s) 1438
BlpI GCTNAGC 1 cut(s) 1531
BlsI GCNGC 7 cut(s) 351, 436, 439, 529, 564, 1286, 1497
Bme1390I CCNGG 3 cut(s) 118, 1709, 1710
Bme18I GGWCC 3 cut(s) 175, 1344, 1546
BmeT110I CYCGRG 4 cut(s) 91, 566, 1259, 1708
BmgT120I GGNCC 4 cut(s) 175, 1344, 1372, 1546
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 118, 1709, 1710
BmsI GCATC 6 cut(s) 549, 722, 917, 974, 1458, 1507
BpiI GAAGAC 1 cut(s) 1412
Bpu1102I GCTNAGC 1 cut(s) 1531
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 1709, 1710
BsaAI YACGTR 1 cut(s) 538
BsaJI CCNNGG 6 cut(s) 63, 117, 1368, 1438, 1707, 1708
BsaWI WCCGGW 1 cut(s) 399
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 5 cut(s) 699, 1658, 1659, 1660, 1709
Bse1I ACTGG 3 cut(s) 466, 693, 1519
Bse21I CCTNAGG 1 cut(s) 1483
Bse3DI GCAATG 2 cut(s) 306, 796
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 6 cut(s) 63, 117, 1368, 1438, 1707, 1708
BseGI GGATG 8 cut(s) 507, 737, 871, 950, 965, 1017, 1364, 1695
BseLI CCNNNNNNNGG 5 cut(s) 699, 1658, 1659, 1660, 1709
BseMI GCAATG 2 cut(s) 306, 796
BseMII CTCAG 4 cut(s) 1334, 1497, 1545, 1592
BseNI ACTGG 3 cut(s) 466, 693, 1519
BseRI GAGGAG 5 cut(s) 389, 392, 395, 398, 1465
BseXI GCAGC 6 cut(s) 336, 421, 424, 549, 1296, 1482
BseYI CCCAGC 2 cut(s) 19, 44
BsgI GTGCAG 4 cut(s) 164, 488, 837, 1303
Bsh1236I CGCG 1 cut(s) 1148
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 1016, 1034, 1117, 1373
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 3 cut(s) 102, 688, 1239
BsiHKCI CYCGRG 4 cut(s) 91, 566, 1259, 1708
BsiSI CCGG 2 cut(s) 400, 1709
BslFI GGGAC 1 cut(s) 1408
BslI CCNNNNNNNGG 5 cut(s) 699, 1658, 1659, 1660, 1709
BsmAI GTCTC 3 cut(s) 397, 602, 1547
BsmFI GGGAC 1 cut(s) 1408
BsmI GAATGC 1 cut(s) 1163
BsnI GGCC 4 cut(s) 1016, 1034, 1117, 1373
BsoBI CYCGRG 4 cut(s) 91, 566, 1259, 1708
Bsp1286I GDGCHC 3 cut(s) 102, 688, 1239
Bsp1720I GCTNAGC 1 cut(s) 1531
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 2 cut(s) 528, 1394
BspANI GGCC 4 cut(s) 1016, 1034, 1117, 1373
BspCNI CTCAG 4 cut(s) 1333, 1496, 1544, 1591
BspFNI CGCG 1 cut(s) 1148
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 1711
BspPI GGATC 7 cut(s) 277, 635, 967, 1096, 1556, 1568, 1626
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 1376
BsrDI GCAATG 2 cut(s) 306, 796
BsrI ACTGG 3 cut(s) 466, 693, 1519
BssECI CCNNGG 6 cut(s) 63, 117, 1368, 1438, 1707, 1708
BssT1I CCWWGG 3 cut(s) 63, 1368, 1438
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 4 cut(s) 107, 484, 548, 805
BstAFI CTTAAG 1 cut(s) 1376
BstAPI GCANNNNNTGC 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 538
BstC8I GCNNGC 3 cut(s) 951, 1056, 1239
BstDEI CTNAG 7 cut(s) 523, 705, 1026, 1320, 1483, 1531, 1578
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 8 cut(s) 507, 737, 871, 950, 965, 1017, 1364, 1695
BstFNI CGCG 1 cut(s) 1148
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 4 cut(s) 223, 789, 1150, 1473
BstMAI GTCTC 3 cut(s) 397, 602, 1547
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 68, 502, 571
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 1058
BstSCI CCNGG 3 cut(s) 116, 1707, 1708
BstUI CGCG 1 cut(s) 1148
BstV1I GCAGC 6 cut(s) 336, 421, 424, 549, 1296, 1482
BstV2I GAAGAC 1 cut(s) 1412
BstX2I RGATCY 2 cut(s) 425, 1088
BstYI RGATCY 2 cut(s) 425, 1088
Bsu36I CCTNAGG 1 cut(s) 1483
BsuI GTATCC 2 cut(s) 49, 458
BsuRI GGCC 4 cut(s) 1016, 1034, 1117, 1373
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 1484
BtsCI GGATG 8 cut(s) 507, 737, 871, 950, 965, 1017, 1364, 1695
BtsI GCAGTG 1 cut(s) 782
BtsIMutI CAGTG 2 cut(s) 782, 801
BveI ACCTGC 1 cut(s) 1711
Cac8I GCNNGC 3 cut(s) 951, 1056, 1239
CaiI CAGNNNCTG 1 cut(s) 699
CfoI GCGC 4 cut(s) 223, 789, 1150, 1473
Cfr13I GGNCC 4 cut(s) 175, 1344, 1372, 1546
Cfr9I CCCGGG 1 cut(s) 1708
CseI GACGC 1 cut(s) 1417
Csp6I GTAC 4 cut(s) 88, 108, 415, 1082
CviAII CATG 8 cut(s) 64, 354, 1055, 1223, 1336, 1390, 1550, 1722
CviQI GTAC 4 cut(s) 88, 108, 415, 1082
DdeI CTNAG 7 cut(s) 523, 705, 1026, 1320, 1483, 1531, 1578
DrdI GACNNNNNNGTC 1 cut(s) 875
DseDI GACNNNNNNGTC 1 cut(s) 875
EaeI YGGCCR 1 cut(s) 1032
Ecl136II GAGCTC 2 cut(s) 100, 1237
Eco130I CCWWGG 3 cut(s) 63, 1368, 1438
Eco24I GRGCYC 2 cut(s) 102, 1239
Eco32I GATATC 1 cut(s) 672
Eco47I GGWCC 3 cut(s) 175, 1344, 1546
Eco47III AGCGCT 1 cut(s) 222
Eco53kI GAGCTC 2 cut(s) 100, 1237
Eco57I CTGAAG 1 cut(s) 995
Eco72I CACGTG 1 cut(s) 538
Eco81I CCTNAGG 1 cut(s) 1483
Eco88I CYCGRG 4 cut(s) 91, 566, 1259, 1708
EcoICRI GAGCTC 2 cut(s) 100, 1237
EcoO109I RGGNCCY 2 cut(s) 175, 1372
EcoRII CCWGG 1 cut(s) 116
EcoRV GATATC 1 cut(s) 672
EcoT14I CCWWGG 3 cut(s) 63, 1368, 1438
EcoT38I GRGCYC 2 cut(s) 102, 1239
ErhI CCWWGG 3 cut(s) 63, 1368, 1438
FaeI CATG 8 cut(s) 67, 357, 1058, 1226, 1339, 1393, 1553, 1725
FaqI GGGAC 1 cut(s) 1408
FatI CATG 8 cut(s) 63, 353, 1054, 1222, 1335, 1389, 1549, 1721
Fnu4HI GCNGC 7 cut(s) 350, 435, 438, 528, 563, 1285, 1496
FokI GGATG 8 cut(s) 494, 744, 878, 952, 957, 1004, 1351, 1682
FriOI GRGCYC 2 cut(s) 102, 1239
Fsp4HI GCNGC 7 cut(s) 350, 435, 438, 528, 563, 1285, 1496
FspBI CTAG 4 cut(s) 266, 393, 1079, 1439
FspI TGCGCA 1 cut(s) 788
GlaI GCGC 4 cut(s) 222, 788, 1149, 1472
GluI GCNGC 7 cut(s) 350, 435, 438, 528, 563, 1285, 1496
GsaI CCCAGC 2 cut(s) 23, 48
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 4 cut(s) 1016, 1034, 1117, 1373
HapII CCGG 2 cut(s) 400, 1709
HgaI GACGC 1 cut(s) 1417
HhaI GCGC 4 cut(s) 223, 789, 1150, 1473
Hin1II CATG 8 cut(s) 67, 357, 1058, 1226, 1339, 1393, 1553, 1725
Hin6I GCGC 4 cut(s) 221, 787, 1148, 1471
HinP1I GCGC 4 cut(s) 221, 787, 1148, 1471
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HpaII CCGG 2 cut(s) 400, 1709
HphI GGTGA 5 cut(s) 389, 656, 1408, 1457, 1575
Hpy166II GTNNAC 3 cut(s) 110, 390, 1331
Hpy188I TCNGA 7 cut(s) 445, 656, 731, 1093, 1158, 1323, 1561
Hpy8I GTNNAC 3 cut(s) 110, 390, 1331
Hpy99I CGWCG 2 cut(s) 884, 1661
HpyAV CCTTC 4 cut(s) 166, 235, 654, 1596
HpyCH4III ACNGT 4 cut(s) 107, 484, 548, 805
HpyCH4IV ACGT 1 cut(s) 537
HpyCH4V TGCA 9 cut(s) 181, 338, 456, 496, 505, 818, 1046, 1284, 1498
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 502, 571
HpyF3I CTNAG 7 cut(s) 523, 705, 1026, 1320, 1483, 1531, 1578
HpySE526I ACGT 1 cut(s) 537
Hsp92II CATG 8 cut(s) 67, 357, 1058, 1226, 1339, 1393, 1553, 1725
HspAI GCGC 4 cut(s) 221, 787, 1148, 1471
LmnI GCTCC 5 cut(s) 68, 97, 933, 1457, 1478
Lsp1109I GCAGC 6 cut(s) 336, 421, 424, 549, 1296, 1482
LweI GCATC 6 cut(s) 549, 722, 917, 974, 1458, 1507
MaeI CTAG 4 cut(s) 266, 393, 1079, 1439
MaeII ACGT 1 cut(s) 537
MaeIII GTNAC 5 cut(s) 302, 395, 1315, 1517, 1581
MboII GAAGA 3 cut(s) 511, 709, 1417
MfeI CAATTG 1 cut(s) 1121
MflI RGATCY 2 cut(s) 425, 1088
MhlI GDGCHC 3 cut(s) 102, 688, 1239
MlsI TGGCCA 1 cut(s) 1034
MluCI AATT 9 cut(s) 746, 827, 987, 1041, 1121, 1170, 1228, 1254, 1623
MluNI TGGCCA 1 cut(s) 1034
MlyI GAGTC 2 cut(s) 395, 399
MmeI TCCRAC 3 cut(s) 169, 660, 979
Mox20I TGGCCA 1 cut(s) 1034
MroXI GAANNNNTTC 1 cut(s) 1163
MscI TGGCCA 1 cut(s) 1034
MseI TTAA 8 cut(s) 246, 486, 779, 831, 933, 939, 1131, 1377
MslI CAYNNNNRTG 2 cut(s) 813, 1394
Msp20I TGGCCA 1 cut(s) 1034
MspCI CTTAAG 1 cut(s) 1376
MspI CCGG 2 cut(s) 400, 1709
MspR9I CCNGG 3 cut(s) 118, 1709, 1710
MunI CAATTG 1 cut(s) 1121
Mva1269I GAATGC 1 cut(s) 1163
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 1148
MwoI GCNNNNNNNGC 3 cut(s) 68, 502, 571
NciI CCSGG 2 cut(s) 1709, 1710
NcoI CCATGG 1 cut(s) 63
NlaIII CATG 8 cut(s) 67, 357, 1058, 1226, 1339, 1393, 1553, 1725
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 3 cut(s) 395, 1517, 1581
NsbI TGCGCA 1 cut(s) 788
NspI RCATGY 1 cut(s) 1058
PaeI GCATGC 1 cut(s) 1058
PaeR7I CTCGAG 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 1711
PctI GAATGC 1 cut(s) 1163
PdmI GAANNNNTTC 1 cut(s) 1163
PfeI GAWTC 8 cut(s) 230, 262, 580, 651, 709, 795, 1072, 1126
PflMI CCANNNNNTGG 1 cut(s) 699
PkrI GCNGC 7 cut(s) 351, 436, 439, 529, 564, 1286, 1497
PleI GAGTC 2 cut(s) 394, 399
PmaCI CACGTG 1 cut(s) 538
PmlI CACGTG 1 cut(s) 538
PpsI GAGTC 2 cut(s) 394, 399
Ppu21I YACGTR 1 cut(s) 538
PpuMI RGGWCCY 1 cut(s) 175
PsiI TTATAA 3 cut(s) 450, 479, 986
Psp124BI GAGCTC 2 cut(s) 102, 1239
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 116
PspCI CACGTG 1 cut(s) 538
PspFI CCCAGC 2 cut(s) 19, 44
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 4 cut(s) 175, 1344, 1372, 1546
PspPPI RGGWCCY 1 cut(s) 175
PspXI VCTCGAGB 1 cut(s) 91
PstNI CAGNNNCTG 1 cut(s) 699
PsuI RGATCY 2 cut(s) 425, 1088
RsaI GTAC 4 cut(s) 89, 109, 416, 1083
RsaNI GTAC 4 cut(s) 88, 108, 415, 1082
RseI CAYNNNNRTG 2 cut(s) 813, 1394
SacI GAGCTC 2 cut(s) 102, 1239
SaqAI TTAA 8 cut(s) 246, 486, 779, 831, 933, 939, 1131, 1377
SatI GCNGC 7 cut(s) 350, 435, 438, 528, 563, 1285, 1496
Sau96I GGNCC 4 cut(s) 175, 1344, 1372, 1546
SchI GAGTC 2 cut(s) 395, 399
ScrFI CCNGG 3 cut(s) 118, 1709, 1710
SduI GDGCHC 3 cut(s) 102, 688, 1239
SfaNI GCATC 6 cut(s) 549, 722, 917, 974, 1458, 1507
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 3 cut(s) 175, 1344, 1546
SlaI CTCGAG 1 cut(s) 91
SmaI CCCGGG 1 cut(s) 1710
SmiMI CAYNNNNRTG 2 cut(s) 813, 1394
SmlI CTYRAG 3 cut(s) 91, 236, 1376
SmoI CTYRAG 3 cut(s) 91, 236, 1376
SpeI ACTAGT 1 cut(s) 392
SphI GCATGC 1 cut(s) 1058
Sse9I AATT 9 cut(s) 746, 827, 987, 1041, 1121, 1170, 1228, 1254, 1623
SsiI CCGC 2 cut(s) 528, 1394
SspI AATATT 2 cut(s) 1100, 1675
SspMI CTAG 4 cut(s) 266, 393, 1079, 1439
SstI GAGCTC 2 cut(s) 102, 1239
StyD4I CCNGG 3 cut(s) 116, 1707, 1708
StyI CCWWGG 3 cut(s) 63, 1368, 1438
TaaI ACNGT 4 cut(s) 107, 484, 548, 805
TaiI ACGT 1 cut(s) 540
TaqI TCGA 6 cut(s) 92, 102, 583, 647, 742, 1233
TasI AATT 9 cut(s) 746, 827, 987, 1041, 1121, 1170, 1228, 1254, 1623
TatI WGTACW 1 cut(s) 1081
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 8 cut(s) 230, 262, 580, 651, 709, 795, 1072, 1126
Tru1I TTAA 8 cut(s) 246, 486, 779, 831, 933, 939, 1131, 1377
Tru9I TTAA 8 cut(s) 246, 486, 779, 831, 933, 939, 1131, 1377
TscAI CASTG 2 cut(s) 789, 808
TseFI GTSAC 3 cut(s) 395, 1517, 1581
TseI GCWGC 6 cut(s) 349, 434, 437, 562, 1284, 1495
Tsp45I GTSAC 3 cut(s) 395, 1517, 1581
TspDTI ATGAA 7 cut(s) 787, 1188, 1352, 1371, 1578, 1662, 1684
TspGWI ACGGA 1 cut(s) 1428
TspMI CCCGGG 1 cut(s) 1708
TspRI CASTG 2 cut(s) 789, 808
Van91I CCANNNNNTGG 1 cut(s) 699
Vha464I CTTAAG 1 cut(s) 1376
VpaK11BI GGWCC 3 cut(s) 175, 1344, 1546
XapI RAATTY 1 cut(s) 827
XceI RCATGY 1 cut(s) 1058
XhoI CTCGAG 1 cut(s) 91
XmaI CCCGGG 1 cut(s) 1708
XmaJI CCTAGG 1 cut(s) 1438
XmnI GAANNNNTTC 1 cut(s) 1163
XspI CTAG 4 cut(s) 266, 393, 1079, 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.