FvH4_1g13200

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011037
Physical Location & Seq
Reverse (-)
7259899 .. 7262348
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g13200.t1

Sequence Viewer

Length: 945 bp
ATGGTGAACTTAGTAGCACTACAGAAGCCCTTGCTGCATGGCCTGATGAAAATGGCCGGAGTCGTACCCTACACGGTGGAGATTGAGCCCGGCACGGTGATGAACTTCTGGGTCCCGAAAGAAACCCTAAAGAAGCCGAAGAAGGGAGAGAAGCCCGAGATCATATCCAAACCCAAGAAGCCGGTAGTGGTTCTGATACACGGCTTCGCCGCCGAGGGAATAGTGACGTGGCAGTTTCAGGTGGGTGCGCTGGCCAAGAAATACTCTGTCTACGTTCCCGACGTCCTGTTTTTTGGGGGGTCGACGACCGACAAGCCCGACAGGTCTCCTGCTCTTCAGGCCGAGTGCGTCTCTACGGGGCTGAGGAAGCTCGGGGTGGACACGTGTACGTTGGTGGGGTTCAGTTACGGCGGCATGGTGGCGTTCAAGATGGCGGAGATGTATCCGGAGCTGGTGAGAGCCATGGTGGTCTCCGGGTCGATATTGGCGATGACGTGTTCGGTGAGCGATGATACTATGAAGAGGCTAGGGTTTGAGTCGTCGTCGGAGCTTTTGCTGCCGAGAACGGTGAAGGGGCTCAAGGCTCTGCTGTCGGTGGCGGCTCACAAGAAGCTGTATTTTCCCGATCGGCTCCACAGAGACTTCCTTGAGGTGATGTTCACGAACAGGAAGGAAAGGGAAGAACTACTTGAAGCTTTAGTCATTGACACAAAGGACCCAAACATTCCCAAATTCCCACAGAGAATTCATCTATTATGGGGTGAAAATGATCAGATATTTAAGCAGGAGCTTGCTCACAACATGAAAGAGCAACTAGGAGCAAATGCAACATTTGAAGGCATAAAGAAAGCAGGTCATCTGGTTCACCTTGAAAGACCTTGTGTTTACAACAGGTGTCTCAAGAGATTCCTAGCCTCCCTGAACGATGCTGGAGCTCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

315

Amino Acids

34.99

Weight (kDa)

9.2

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 59 - 292 2.4e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 61 - 176 3.7e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 63 - 294 7.5e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18360 AT2G18360 AT4G36610
fragaria_vesca FvH4_1g13190 FvH4_1g13200
malus_domestica MD02G1146700.v1.1 MD15G1260700.v1.1
prunus_persica Prupe.7G155500_v2.0.a1
pyrus_communis pycom02g11600 pycom15g22790
rosa_chinensis RchiOBHm_Chr2g0101411
rosa_laevigata RLG00000017076
rosa_multiflora Rmu_sc0006128.1_g000006
rosa_roxburghii Rroxscaffold_2G00141330
rosa_rugosa Rorug02G0098400
rosa_samantha Rh2AG146700 Rh2BG152100 Rh2CG152600 Rh2DG152200
rosa_wichuraiana Rw2G011440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 842
AccI GTMKAC 2 cut(s) 270, 302
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 4 cut(s) 210, 411, 434, 599
AcoI YGGCCR 2 cut(s) 54, 252
AcsI RAATTY 2 cut(s) 731, 744
AcuI CTGAAG 1 cut(s) 320
AcvI CACGTG 1 cut(s) 384
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 2 cut(s) 66, 388
AfiI CCNNNNNNNGG 1 cut(s) 143
AflIII ACRYGT 3 cut(s) 381, 383, 494
AgsI TTSAA 4 cut(s) 427, 692, 836, 872
AjiI CACGTC 2 cut(s) 228, 495
AluBI AGCT 7 cut(s) 370, 451, 550, 613, 695, 790, 935
AluI AGCT 7 cut(s) 370, 451, 550, 613, 695, 790, 935
Alw21I GWGCWC 1 cut(s) 937
Alw26I GTCTC 5 cut(s) 330, 355, 475, 633, 902
Ama87I CYCGRG 2 cut(s) 155, 371
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 4 cut(s) 40, 54, 252, 339
ApeKI GCWGC 2 cut(s) 34, 556
ApoI RAATTY 2 cut(s) 731, 744
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 2 cut(s) 112, 715
AsuC2I CCSGG 2 cut(s) 90, 475
AsuHPI GGTGA 8 cut(s) 16, 109, 466, 514, 580, 664, 773, 857
AvaI CYCGRG 2 cut(s) 155, 371
AvaII GGWCC 2 cut(s) 112, 715
BalI TGGCCA 1 cut(s) 254
BanII GRGCYC 3 cut(s) 90, 579, 937
BbrPI CACGTG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 937
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 2 cut(s) 21, 543
BccI CCATC 1 cut(s) 424
BceAI ACGGC 2 cut(s) 217, 424
BciVI GTATCC 1 cut(s) 453
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 2 cut(s) 90, 475
BcoDI GTCTC 5 cut(s) 330, 355, 475, 633, 902
BfaI CTAG 3 cut(s) 527, 815, 911
BfmI CTRYAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 842
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 5 cut(s) 35, 210, 412, 557, 600
BlsI GCNGC 5 cut(s) 36, 211, 413, 558, 601
Bme1390I CCNGG 2 cut(s) 90, 475
Bme18I GGWCC 2 cut(s) 112, 715
BmeT110I CYCGRG 2 cut(s) 155, 371
BmgBI CACGTC 2 cut(s) 228, 495
BmgT120I GGNCC 2 cut(s) 112, 715
BmiI GGNNCC 4 cut(s) 113, 114, 632, 717
BmrFI CCNGG 2 cut(s) 90, 475
BmsI GCATC 1 cut(s) 916
BplI GAGNNNNNCTC 2 cut(s) 335, 367
Bpu10I CCTNAGC 1 cut(s) 362
BpuEI CTTGAG 3 cut(s) 563, 668, 884
BpuMI CCSGG 2 cut(s) 90, 475
BsaAI YACGTR 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 2 cut(s) 330, 475
BsaJI CCNNGG 2 cut(s) 213, 462
BsaWI WCCGGW 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse118I RCCGGY 1 cut(s) 181
BseAI TCCGGA 1 cut(s) 445
BseDI CCNNGG 2 cut(s) 213, 462
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMII CTCAG 1 cut(s) 353
BseXI GCAGC 2 cut(s) 21, 543
Bsh1285I CGRYCG 2 cut(s) 309, 628
BshFI GGCC 4 cut(s) 42, 56, 254, 341
BsiEI CGRYCG 2 cut(s) 309, 628
BsiHKAI GWGCWC 1 cut(s) 937
BsiHKCI CYCGRG 2 cut(s) 155, 371
BsiSI CCGG 5 cut(s) 57, 90, 182, 446, 474
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 5 cut(s) 330, 355, 475, 633, 902
BsmBI CGTCTC 1 cut(s) 355
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 4 cut(s) 42, 56, 254, 341
Bso31I GGTCTC 2 cut(s) 330, 475
BsoBI CYCGRG 2 cut(s) 155, 371
Bsp1286I GDGCHC 3 cut(s) 90, 579, 937
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 3 cut(s) 159, 625, 769
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 4 cut(s) 210, 411, 434, 599
BspANI GGCC 4 cut(s) 42, 56, 254, 341
BspCNI CTCAG 1 cut(s) 354
BspEI TCCGGA 1 cut(s) 445
BspLI GGNNCC 4 cut(s) 113, 114, 632, 717
BspMI ACCTGC 1 cut(s) 842
BspQI GCTCTTC 1 cut(s) 339
BspTNI GGTCTC 2 cut(s) 330, 475
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 213, 462
BssMI GATC 3 cut(s) 159, 625, 769
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 462
Bst4CI ACNGT 3 cut(s) 76, 97, 568
Bst6I CTCTTC 2 cut(s) 339, 515
BstACI GRCGYC 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 252, 792
BstDEI CTNAG 2 cut(s) 10, 362
BstDSI CCRYGG 1 cut(s) 462
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 3 cut(s) 162, 628, 772
BstMAI GTCTC 5 cut(s) 330, 355, 475, 633, 902
BstMBI GATC 3 cut(s) 159, 625, 769
BstMCI CGRYCG 2 cut(s) 309, 628
BstMWI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
BstSCI CCNGG 2 cut(s) 88, 473
BstSFI CTRYAG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 21, 543
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 4 cut(s) 42, 56, 254, 341
BtgI CCRYGG 1 cut(s) 462
BtgZI GCGATG 2 cut(s) 503, 522
BtrI CACGTC 2 cut(s) 228, 495
BveI ACCTGC 1 cut(s) 842
Cac8I GCNNGC 2 cut(s) 252, 792
CfoI GCGC 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 112, 715
CseI GACGC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 65, 387
CviAII CATG 4 cut(s) 38, 415, 463, 802
CviQI GTAC 2 cut(s) 65, 387
DdeI CTNAG 2 cut(s) 10, 362
DpnI GATC 3 cut(s) 161, 627, 771
DpnII GATC 3 cut(s) 159, 625, 769
EaeI YGGCCR 2 cut(s) 54, 252
Eam1104I CTCTTC 2 cut(s) 339, 515
EarI CTCTTC 2 cut(s) 339, 515
EciI GGCGGA 1 cut(s) 449
Ecl136II GAGCTC 1 cut(s) 935
Eco130I CCWWGG 1 cut(s) 462
Eco24I GRGCYC 3 cut(s) 90, 579, 937
Eco31I GGTCTC 2 cut(s) 330, 475
Eco47I GGWCC 2 cut(s) 112, 715
Eco53kI GAGCTC 1 cut(s) 935
Eco57I CTGAAG 1 cut(s) 320
Eco72I CACGTG 1 cut(s) 384
Eco88I CYCGRG 2 cut(s) 155, 371
EcoICRI GAGCTC 1 cut(s) 935
EcoO109I RGGNCCY 2 cut(s) 112, 715
EcoRI GAATTC 1 cut(s) 744
EcoT14I CCWWGG 1 cut(s) 462
EcoT38I GRGCYC 3 cut(s) 90, 579, 937
ErhI CCWWGG 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 355
FaeI CATG 4 cut(s) 41, 418, 466, 805
FaiI YATR 8 cut(s) 39, 164, 416, 464, 518, 757, 803, 842
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 98
FatI CATG 4 cut(s) 37, 414, 462, 801
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 2 cut(s) 270, 302
Fnu4HI GCNGC 5 cut(s) 35, 210, 412, 557, 600
FriOI GRGCYC 3 cut(s) 90, 579, 937
Fsp4HI GCNGC 5 cut(s) 35, 210, 412, 557, 600
FspBI CTAG 3 cut(s) 527, 815, 911
GlaI GCGC 1 cut(s) 249
GluI GCNGC 5 cut(s) 35, 210, 412, 557, 600
HaeIII GGCC 4 cut(s) 42, 56, 254, 341
HapII CCGG 5 cut(s) 57, 90, 182, 446, 474
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 250
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 4 cut(s) 41, 418, 466, 805
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HindIII AAGCTT 1 cut(s) 693
HinfI GANTC 3 cut(s) 60, 536, 906
HpaII CCGG 5 cut(s) 57, 90, 182, 446, 474
HphI GGTGA 8 cut(s) 16, 109, 466, 514, 580, 664, 773, 857
Hpy166II GTNNAC 8 cut(s) 7, 271, 303, 379, 387, 660, 865, 886
Hpy188I TCNGA 3 cut(s) 195, 547, 774
Hpy188III TCNNGA 7 cut(s) 115, 278, 427, 446, 623, 661, 901
Hpy8I GTNNAC 8 cut(s) 7, 271, 303, 379, 387, 660, 865, 886
Hpy99I CGWCG 4 cut(s) 284, 307, 544, 547
HpyAV CCTTC 4 cut(s) 136, 565, 664, 830
HpyCH4III ACNGT 3 cut(s) 76, 97, 568
HpyCH4IV ACGT 6 cut(s) 227, 273, 282, 383, 389, 494
HpyCH4V TGCA 2 cut(s) 37, 827
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
HpyF3I CTNAG 2 cut(s) 10, 362
HpySE526I ACGT 6 cut(s) 227, 273, 282, 383, 389, 494
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 4 cut(s) 41, 418, 466, 805
HspAI GCGC 1 cut(s) 248
KflI GGGWCCC 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 3 cut(s) 159, 625, 769
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 6 cut(s) 448, 547, 636, 787, 818, 932
Lsp1109I GCAGC 2 cut(s) 21, 543
LweI GCATC 1 cut(s) 916
MaeI CTAG 3 cut(s) 527, 815, 911
MaeII ACGT 6 cut(s) 227, 273, 282, 383, 389, 494
MaeIII GTNAC 2 cut(s) 223, 404
MalI GATC 3 cut(s) 161, 627, 771
MboI GATC 3 cut(s) 159, 625, 769
MboII GAAGA 4 cut(s) 151, 326, 532, 692
MhlI GDGCHC 3 cut(s) 90, 579, 937
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 2 cut(s) 731, 744
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 2 cut(s) 69, 545
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 5 cut(s) 208, 357, 516, 643, 925
Mox20I TGGCCA 1 cut(s) 254
MroI TCCGGA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 254
MseI TTAA 2 cut(s) 780, 943
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 5 cut(s) 57, 90, 182, 446, 474
MspR9I CCNGG 2 cut(s) 90, 475
MwoI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
NciI CCSGG 2 cut(s) 90, 475
NcoI CCATGG 1 cut(s) 462
NdeII GATC 3 cut(s) 159, 625, 769
NlaIII CATG 4 cut(s) 41, 418, 466, 805
NlaIV GGNNCC 4 cut(s) 113, 114, 632, 717
NmeAIII GCCGAG 3 cut(s) 238, 367, 585
NmuCI GTSAC 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 2 cut(s) 279, 485
PfeI GAWTC 1 cut(s) 906
PkrI GCNGC 5 cut(s) 36, 211, 413, 558, 601
Ple19I CGATCG 1 cut(s) 628
PleI GAGTC 2 cut(s) 68, 544
PmaCI CACGTG 1 cut(s) 384
PmlI CACGTG 1 cut(s) 384
PpsI GAGTC 2 cut(s) 68, 544
Ppu21I YACGTR 1 cut(s) 384
PpuMI RGGWCCY 2 cut(s) 112, 715
Psp124BI GAGCTC 1 cut(s) 937
Psp5II RGGWCCY 2 cut(s) 112, 715
PspCI CACGTG 1 cut(s) 384
PspN4I GGNNCC 4 cut(s) 113, 114, 632, 717
PspPI GGNCC 2 cut(s) 112, 715
PspPPI RGGWCCY 2 cut(s) 112, 715
PvuI CGATCG 1 cut(s) 628
RsaI GTAC 2 cut(s) 66, 388
RsaNI GTAC 2 cut(s) 65, 387
RseI CAYNNNNRTG 1 cut(s) 98
SacI GAGCTC 1 cut(s) 937
SalI GTCGAC 1 cut(s) 301
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 2 cut(s) 780, 943
SatI GCNGC 5 cut(s) 35, 210, 412, 557, 600
Sau3AI GATC 3 cut(s) 159, 625, 769
Sau96I GGNCC 2 cut(s) 112, 715
SchI GAGTC 2 cut(s) 69, 545
ScrFI CCNGG 2 cut(s) 90, 475
SduI GDGCHC 3 cut(s) 90, 579, 937
SfaNI GCATC 1 cut(s) 916
SfcI CTRYAG 1 cut(s) 20
SinI GGWCC 2 cut(s) 112, 715
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 578, 647, 899
SmoI CTYRAG 3 cut(s) 578, 647, 899
Sse9I AATT 2 cut(s) 731, 744
SsiI CCGC 4 cut(s) 210, 411, 434, 599
SspMI CTAG 3 cut(s) 527, 815, 911
SstI GAGCTC 1 cut(s) 937
StyD4I CCNGG 2 cut(s) 88, 473
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 3 cut(s) 76, 97, 568
TaiI ACGT 6 cut(s) 230, 276, 285, 386, 392, 497
TaqI TCGA 2 cut(s) 302, 479
TaqII GACCGA 1 cut(s) 323
TasI AATT 2 cut(s) 731, 744
TauI GCSGC 3 cut(s) 212, 414, 602
TfiI GAWTC 1 cut(s) 906
Tru1I TTAA 2 cut(s) 780, 943
Tru9I TTAA 2 cut(s) 780, 943
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 34, 556
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 5 cut(s) 62, 116, 533, 737, 818
VpaK11BI GGWCC 2 cut(s) 112, 715
XapI RAATTY 2 cut(s) 731, 744
XmiI GTMKAC 2 cut(s) 270, 302
XspI CTAG 3 cut(s) 527, 815, 911
ZraI GACGTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.