MD15G1260700.v1.1

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011037
Physical Location & Seq
Reverse (-)
22256127 .. 22262175
6049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1260700.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGTGAACCTAGTGGCACTTCAGCAACCCTTGCTGCATGGCCTTATGAAAATGGCCGGGGTCCGACCTTACGCGGTCCAGATCGAACCGTGCACGGTTATGAATTTCTGGGTCCCGAACGAGACCCTCAACAAACCCAAGAAGGGGGAAAAACCCGCTATGGTGTCGGGTCCAACAAAGCCCGTGGTGGTCCTCATCCATGGGTTCGCGGCCGAGGGGATAGTGACGTGGCAGTTCCAGGTTGGTGCTTTGACCAAAAAATACTCCGTCTATGTCCCCGACCTCCTCTTCTTCGGCGGCTCCTCCACCGACAACCCCGACCGGTCGCCGACGTTCCAGGCGGAGTGTGTGGCCGCGGGGTTGAGGAAGCTCGGGGTGGAGAGGTGCGCGGTGGTTGGGTTTAGTTACGGCGGGATGGTGGCGTTTAAGTTGGCCGAGCTGTACCCGGACCTGGTGGAGTCCATGGTAGTATCCGGATCGATCTTGGCCATGACTTGCTCAGTCAGCGATGCAACGATGGAGAGGCTCGGGTTTTCGTCGTCGTCGGAGCTTCTACTGCCAAGCTCGGTGAAGGGGCTCAAGGCTCTGCTGTCTGTTGCTGCTCATAAGAAGCTGTATTTTCCTGATCGGCTTCACAAGGACTTCCTTGAGGTGATGTTCACCCACAGGAAGGAAAGGGCAGAACTCCTTGAAGCTTTAGTCATTGATACCAAGGACCCAACCATCCCCAAATTCCCACAGAGAATACATCTGTTATGGGGTGAAAATGATCAGATATTCAACCAGGAGCTTGCCCACAACATGAAAGAGCAATTAGGAGAAAATGCAACATTTGAAGGCATAAAGAAGGCAGGCCACTTGGTTCATTTGGAACGGCCCTGTGTTTACAACAGGTGTCTCAAGCGATTTCTTGCTTCCCTGCAGCACGAAGAATATGGAGCCCCAAAAAAGGGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

320

Amino Acids

35.39

Weight (kDa)

8.62

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 59 - 292 2.5e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 61 - 178 3.7e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 63 - 294 9e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18360 AT2G18360 AT4G36610
fragaria_vesca FvH4_1g13190 FvH4_1g13200
malus_domestica MD02G1146700.v1.1 MD15G1260700.v1.1
prunus_persica Prupe.7G155500_v2.0.a1
pyrus_communis pycom02g11600 pycom15g22790
rosa_chinensis RchiOBHm_Chr2g0101411
rosa_laevigata RLG00000017076
rosa_multiflora Rmu_sc0006128.1_g000006
rosa_roxburghii Rroxscaffold_2G00141330
rosa_rugosa Rorug02G0098400
rosa_samantha Rh2AG146700 Rh2BG152100 Rh2CG152600 Rh2DG152200
rosa_wichuraiana Rw2G011440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 74, 209, 356, 389
AccIII TCCGGA 1 cut(s) 473
AciI CCGC 9 cut(s) 74, 156, 209, 297, 341, 354, 356, 389, 411
AclWI GGATC 1 cut(s) 484
AcoI YGGCCR 5 cut(s) 54, 210, 351, 432, 486
AcsI RAATTY 2 cut(s) 103, 731
AcuI CTGAAG 1 cut(s) 5
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 6 cut(s) 143, 144, 451, 670, 949, 950
AgeI ACCGGT 1 cut(s) 321
AgsI TTSAA 3 cut(s) 692, 781, 836
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 4 cut(s) 237, 336, 450, 783
AluBI AGCT 7 cut(s) 370, 439, 550, 564, 613, 695, 790
AluI AGCT 7 cut(s) 370, 439, 550, 564, 613, 695, 790
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 116, 902
Alw44I GTGCAC 1 cut(s) 91
AlwI GGATC 1 cut(s) 484
Ama87I CYCGRG 2 cut(s) 371, 527
Aor13HI TCCGGA 1 cut(s) 473
AoxI GGCC 8 cut(s) 40, 54, 210, 351, 432, 486, 853, 875
ApaLI GTGCAC 1 cut(s) 91
ApeKI GCWGC 3 cut(s) 34, 599, 922
ApoI RAATTY 2 cut(s) 103, 731
AsiGI ACCGGT 1 cut(s) 321
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 8 cut(s) 61, 76, 112, 170, 190, 448, 715, 876
AsuC2I CCSGG 2 cut(s) 58, 446
AsuHPI GGTGA 5 cut(s) 16, 580, 652, 664, 773
AvaI CYCGRG 2 cut(s) 371, 527
AvaII GGWCC 7 cut(s) 61, 76, 112, 170, 190, 448, 715
BaeGI GKGCMC 1 cut(s) 95
BalI TGGCCA 1 cut(s) 488
BanII GRGCYC 2 cut(s) 579, 943
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 3 cut(s) 21, 586, 934
BccI CCATC 3 cut(s) 409, 511, 731
BceAI ACGGC 2 cut(s) 424, 890
BciT130I CCWGG 4 cut(s) 239, 338, 452, 785
BciVI GTATCC 1 cut(s) 481
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 2 cut(s) 58, 446
BcoDI GTCTC 2 cut(s) 116, 902
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 920
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 6 cut(s) 35, 210, 298, 354, 600, 923
BlsI GCNGC 6 cut(s) 36, 211, 299, 355, 601, 924
Bme1390I CCNGG 6 cut(s) 58, 239, 338, 446, 452, 785
Bme18I GGWCC 7 cut(s) 61, 76, 112, 170, 190, 448, 715
BmeT110I CYCGRG 2 cut(s) 371, 527
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 8 cut(s) 61, 76, 112, 170, 190, 448, 715, 876
BmiI GGNNCC 7 cut(s) 62, 113, 114, 171, 301, 717, 940
BmrFI CCNGG 6 cut(s) 58, 239, 338, 446, 452, 785
BmsI GCATC 1 cut(s) 499
BpuEI CTTGAG 3 cut(s) 563, 668, 884
BpuMI CCSGG 2 cut(s) 58, 446
Bsa29I ATCGAT 1 cut(s) 479
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 7 cut(s) 57, 183, 199, 213, 354, 462, 711
BsaWI WCCGGW 2 cut(s) 321, 473
Bsc4I CCNNNNNNNGG 6 cut(s) 143, 144, 451, 670, 949, 950
Bse118I RCCGGY 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 473
BseBI CCWGG 4 cut(s) 239, 338, 452, 785
BseCI ATCGAT 1 cut(s) 479
BseDI CCNNGG 7 cut(s) 57, 183, 199, 213, 354, 462, 711
BseGI GGATG 3 cut(s) 195, 420, 723
BseLI CCNNNNNNNGG 6 cut(s) 143, 144, 451, 670, 949, 950
BseMII CTCAG 1 cut(s) 513
BseRI GAGGAG 2 cut(s) 275, 292
BseSI GKGCMC 1 cut(s) 95
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 3 cut(s) 21, 586, 934
Bsh1236I CGCG 4 cut(s) 74, 209, 356, 389
Bsh1285I CGRYCG 3 cut(s) 213, 322, 326
BshFI GGCC 8 cut(s) 42, 56, 212, 353, 434, 488, 855, 877
BshTI ACCGGT 1 cut(s) 321
BshVI ATCGAT 1 cut(s) 479
BsiEI CGRYCG 3 cut(s) 213, 322, 326
BsiHKAI GWGCWC 1 cut(s) 95
BsiHKCI CYCGRG 2 cut(s) 371, 527
BsiSI CCGG 4 cut(s) 57, 322, 446, 474
BslFI GGGAC 2 cut(s) 98, 260
BslI CCNNNNNNNGG 6 cut(s) 143, 144, 451, 670, 949, 950
BsmAI GTCTC 2 cut(s) 116, 902
BsmFI GGGAC 2 cut(s) 98, 260
BsnI GGCC 8 cut(s) 42, 56, 212, 353, 434, 488, 855, 877
Bso31I GGTCTC 1 cut(s) 116
BsoBI CYCGRG 2 cut(s) 371, 527
Bsp1286I GDGCHC 3 cut(s) 95, 579, 943
Bsp13I TCCGGA 1 cut(s) 473
Bsp143I GATC 5 cut(s) 81, 476, 480, 625, 769
Bsp19I CCATGG 2 cut(s) 199, 462
BspACI CCGC 9 cut(s) 74, 156, 209, 297, 341, 354, 356, 389, 411
BspANI GGCC 8 cut(s) 42, 56, 212, 353, 434, 488, 855, 877
BspCNI CTCAG 1 cut(s) 512
BspDI ATCGAT 1 cut(s) 479
BspEI TCCGGA 1 cut(s) 473
BspFNI CGCG 4 cut(s) 74, 209, 356, 389
BspLI GGNNCC 7 cut(s) 62, 113, 114, 171, 301, 717, 940
BspMAI CTGCAG 1 cut(s) 924
BspPI GGATC 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 116
BsrFI RCCGGY 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 321
BssECI CCNNGG 7 cut(s) 57, 183, 199, 213, 354, 462, 711
BssMI GATC 5 cut(s) 81, 476, 480, 625, 769
BssT1I CCWWGG 3 cut(s) 199, 462, 711
Bst2UI CCWGG 4 cut(s) 239, 338, 452, 785
Bst4CI ACNGT 2 cut(s) 90, 97
Bst6I CTCTTC 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 792, 853
BstDEI CTNAG 1 cut(s) 499
BstDSI CCRYGG 4 cut(s) 183, 199, 354, 462
BstF5I GGATG 3 cut(s) 195, 420, 723
BstFNI CGCG 4 cut(s) 74, 209, 356, 389
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 5 cut(s) 84, 479, 483, 628, 772
BstMAI GTCTC 2 cut(s) 116, 902
BstMBI GATC 5 cut(s) 81, 476, 480, 625, 769
BstMCI CGRYCG 3 cut(s) 213, 322, 326
BstMWI GCNNNNNNNGC 3 cut(s) 31, 504, 556
BstNI CCWGG 4 cut(s) 239, 338, 452, 785
BstSCI CCNGG 6 cut(s) 56, 237, 336, 444, 450, 783
BstSFI CTRYAG 1 cut(s) 920
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 4 cut(s) 74, 209, 356, 389
BstV1I GCAGC 3 cut(s) 21, 586, 934
BstZI CGGCCG 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 479
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 8 cut(s) 42, 56, 212, 353, 434, 488, 855, 877
BsuTUI ATCGAT 1 cut(s) 479
BtgI CCRYGG 4 cut(s) 183, 199, 354, 462
BtgZI GCGATG 1 cut(s) 522
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 3 cut(s) 195, 420, 723
Cac8I GCNNGC 2 cut(s) 792, 853
CfoI GCGC 1 cut(s) 389
Cfr10I RCCGGY 1 cut(s) 321
Cfr13I GGNCC 8 cut(s) 61, 76, 112, 170, 190, 448, 715, 876
Cfr42I CCGCGG 1 cut(s) 357
ClaI ATCGAT 1 cut(s) 479
CsiI ACCWGGT 1 cut(s) 450
Csp6I GTAC 1 cut(s) 442
CspAI ACCGGT 1 cut(s) 321
CspCI CAANNNNNGTGG 2 cut(s) 165, 200
CviAII CATG 5 cut(s) 38, 200, 463, 490, 802
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 1 cut(s) 499
DpnI GATC 5 cut(s) 83, 478, 482, 627, 771
DpnII GATC 5 cut(s) 81, 476, 480, 625, 769
EaeI YGGCCR 5 cut(s) 54, 210, 351, 432, 486
EagI CGGCCG 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 210
Eco130I CCWWGG 3 cut(s) 199, 462, 711
Eco24I GRGCYC 2 cut(s) 579, 943
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 7 cut(s) 61, 76, 112, 170, 190, 448, 715
Eco52I CGGCCG 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 5
Eco88I CYCGRG 2 cut(s) 371, 527
EcoO109I RGGNCCY 2 cut(s) 112, 715
EcoRII CCWGG 4 cut(s) 237, 336, 450, 783
EcoT14I CCWWGG 3 cut(s) 199, 462, 711
EcoT38I GRGCYC 2 cut(s) 579, 943
ErhI CCWWGG 3 cut(s) 199, 462, 711
FaeI CATG 5 cut(s) 41, 203, 466, 493, 805
FaqI GGGAC 2 cut(s) 98, 260
FatI CATG 5 cut(s) 37, 199, 462, 489, 801
FauI CCCGC 3 cut(s) 163, 349, 404
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 6 cut(s) 35, 210, 298, 354, 600, 923
FokI GGATG 3 cut(s) 182, 427, 710
FriOI GRGCYC 2 cut(s) 579, 943
Fsp4HI GCNGC 6 cut(s) 35, 210, 298, 354, 600, 923
FspBI CTAG 1 cut(s) 11
GlaI GCGC 1 cut(s) 388
GluI GCNGC 6 cut(s) 35, 210, 298, 354, 600, 923
HaeIII GGCC 8 cut(s) 42, 56, 212, 353, 434, 488, 855, 877
HapII CCGG 4 cut(s) 57, 322, 446, 474
HhaI GCGC 1 cut(s) 389
Hin1II CATG 5 cut(s) 41, 203, 466, 493, 805
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HindIII AAGCTT 1 cut(s) 693
HinfI GANTC 1 cut(s) 458
HpaII CCGG 4 cut(s) 57, 322, 446, 474
HphI GGTGA 5 cut(s) 16, 580, 652, 664, 773
Hpy166II GTNNAC 4 cut(s) 7, 93, 660, 886
Hpy188I TCNGA 3 cut(s) 65, 547, 774
Hpy188III TCNNGA 4 cut(s) 79, 115, 474, 623
Hpy8I GTNNAC 4 cut(s) 7, 93, 660, 886
Hpy99I CGWCG 4 cut(s) 334, 541, 544, 547
HpyAV CCTTC 5 cut(s) 136, 565, 664, 830, 841
HpyCH4III ACNGT 2 cut(s) 90, 97
HpyCH4IV ACGT 2 cut(s) 227, 332
HpyCH4V TGCA 5 cut(s) 37, 93, 512, 827, 922
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 504, 556
HpyF3I CTNAG 1 cut(s) 499
HpySE526I ACGT 2 cut(s) 227, 332
Hsp92II CATG 5 cut(s) 41, 203, 466, 493, 805
HspAI GCGC 1 cut(s) 387
KflI GGGWCCC 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 473
Ksp22I TGATCA 1 cut(s) 769
KspI CCGCGG 1 cut(s) 357
Kzo9I GATC 5 cut(s) 81, 476, 480, 625, 769
LmnI GCTCC 4 cut(s) 305, 547, 787, 938
Lsp1109I GCAGC 3 cut(s) 21, 586, 934
LweI GCATC 1 cut(s) 499
MabI ACCWGGT 1 cut(s) 450
MaeI CTAG 1 cut(s) 11
MaeII ACGT 2 cut(s) 227, 332
MaeIII GTNAC 2 cut(s) 223, 404
MalI GATC 5 cut(s) 83, 478, 482, 627, 771
MboI GATC 5 cut(s) 81, 476, 480, 625, 769
MboII GAAGA 3 cut(s) 280, 283, 941
MhlI GDGCHC 3 cut(s) 95, 579, 943
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 3 cut(s) 103, 731, 812
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 3 cut(s) 88, 197, 525
Mox20I TGGCCA 1 cut(s) 488
MroI TCCGGA 1 cut(s) 473
MscI TGGCCA 1 cut(s) 488
MseI TTAA 1 cut(s) 426
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 4 cut(s) 57, 322, 446, 474
MspR9I CCNGG 6 cut(s) 58, 239, 338, 446, 452, 785
MvaI CCWGG 4 cut(s) 239, 338, 452, 785
MvnI CGCG 4 cut(s) 74, 209, 356, 389
MwoI GCNNNNNNNGC 3 cut(s) 31, 504, 556
NciI CCSGG 2 cut(s) 58, 446
NcoI CCATGG 2 cut(s) 199, 462
NdeII GATC 5 cut(s) 81, 476, 480, 625, 769
NlaIII CATG 5 cut(s) 41, 203, 466, 493, 805
NlaIV GGNNCC 7 cut(s) 62, 113, 114, 171, 301, 717, 940
NmeAIII GCCGAG 2 cut(s) 238, 460
NmuCI GTSAC 1 cut(s) 223
PinAI ACCGGT 1 cut(s) 321
PkrI GCNGC 6 cut(s) 36, 211, 299, 355, 601, 924
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PpuMI RGGWCCY 2 cut(s) 112, 715
Psp5II RGGWCCY 2 cut(s) 112, 715
Psp6I CCWGG 4 cut(s) 237, 336, 450, 783
PspGI CCWGG 4 cut(s) 237, 336, 450, 783
PspN4I GGNNCC 7 cut(s) 62, 113, 114, 171, 301, 717, 940
PspPI GGNCC 8 cut(s) 61, 76, 112, 170, 190, 448, 715, 876
PspPPI RGGWCCY 2 cut(s) 112, 715
PstI CTGCAG 1 cut(s) 924
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 98
SacII CCGCGG 1 cut(s) 357
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 6 cut(s) 35, 210, 298, 354, 600, 923
Sau3AI GATC 5 cut(s) 81, 476, 480, 625, 769
Sau96I GGNCC 8 cut(s) 61, 76, 112, 170, 190, 448, 715, 876
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 6 cut(s) 58, 239, 338, 446, 452, 785
SduI GDGCHC 3 cut(s) 95, 579, 943
SexAI ACCWGGT 1 cut(s) 450
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 1 cut(s) 920
Sfr303I CCGCGG 1 cut(s) 357
SgrBI CCGCGG 1 cut(s) 357
SinI GGWCC 7 cut(s) 61, 76, 112, 170, 190, 448, 715
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 578, 647, 899
SmoI CTYRAG 3 cut(s) 578, 647, 899
Sse9I AATT 3 cut(s) 103, 731, 812
SsiI CCGC 9 cut(s) 74, 156, 209, 297, 341, 354, 356, 389, 411
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 6 cut(s) 56, 237, 336, 444, 450, 783
StyI CCWWGG 3 cut(s) 199, 462, 711
TaaI ACNGT 2 cut(s) 90, 97
TaiI ACGT 2 cut(s) 230, 335
TaqI TCGA 2 cut(s) 84, 479
TasI AATT 3 cut(s) 103, 731, 812
TauI GCSGC 3 cut(s) 212, 300, 356
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 3 cut(s) 34, 599, 922
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 4 cut(s) 62, 116, 818, 854
TspGWI ACGGA 1 cut(s) 256
VneI GTGCAC 1 cut(s) 91
VpaK11BI GGWCC 7 cut(s) 61, 76, 112, 170, 190, 448, 715
XapI RAATTY 2 cut(s) 103, 731
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.