MD02G1146700.v1.1

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011037
Physical Location & Seq
Reverse (-)
12114854 .. 12116600
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1146700.v1.1.491

Sequence Viewer

Length: 954 bp
ATGGTGAACTTAGTGGCACTTCAGCAACCCTTGCTGCATGGCCTTATGAAAATGGCGGGGGTCCGACCCTACGCGGTCGAAATCGAACCGGGCACGGTCATGAACTTCTGGGTCCCGAACAAGACCCTCAACAAACCCAAGAAGGGCGAAAAGCCTGCTATGGTGTCGGGCCCAACAAAGCCCGTGGTGGTTCTCATCCACGGGTTCGCGGCCGAGGGAATAGTGACGTGGCAGTTCCAGGTTGGCGCTTTGACAAAAAAATACTCCGTCTACGTCCCCGACCTCCTCTTCTTCGGCGGCTCTTCTACCGACAACCCTGACCGCTCGCCGACGTTCCAAGCGGAGTGTGTGGCTGCGGGGTTGAAGAAGCTCGGGGTGGAAAAGTGCGTGGTGGTTGGGTTTAGTTACGGCGGGATGGTGGCGTTTATGCTGGCCGATCTGTACCCGGACCTGGTGCGGTCCATGATAGTGTCCGGTTCGATCCTGGGCATGACTCACTCGTACCGCGACGCTATCATGAAGAGGCTCGGGTTCTCATCGTCGTCGGAGCTTCTGCTGCCGAGCTCGGTGAAGGGGCTCAAGGCTCTGCTGTCTGTTGCTGCTCACAAGAAGCTGTATTTTCCTGATCGGCTTCACAAGGACTTCCTTGAGGTGATGTTCACCCACAGGAAGGAGAGGTCAGAACTCCTTGAAGCTGTAGTCAATGATACAAAGCACACAACCATCCCTAAATTCCCACAGAGAATACATCTGTTATGGGGTGAAAATGATCAGATATTCAACCAGGAGCTTGCCCAAAACATGAAAGAGCAACTAGGAGCAAATGCAACGTTTGAAGGCATAAAGAAGGCAGGCCACTTGGTTCATTTGGAACGACCTTGTGTCTACAACAGGCGTCTCAAGCGATTTCTTGCTTCCCTGCAGCATGAAGAAGACGGAGCCCCAAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

318

Amino Acids

35.29

Weight (kDa)

9.45

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 58 - 292 1.9e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 61 - 293 1.2e-23 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 63 - 295 7.8e-18 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18360 AT2G18360 AT4G36610
fragaria_vesca FvH4_1g13190 FvH4_1g13200
malus_domestica MD02G1146700.v1.1 MD15G1260700.v1.1
prunus_persica Prupe.7G155500_v2.0.a1
pyrus_communis pycom02g11600 pycom15g22790
rosa_chinensis RchiOBHm_Chr2g0101411
rosa_laevigata RLG00000017076
rosa_multiflora Rmu_sc0006128.1_g000006
rosa_roxburghii Rroxscaffold_2G00141330
rosa_rugosa Rorug02G0098400
rosa_samantha Rh2AG146700 Rh2BG152100 Rh2CG152600 Rh2DG152200
rosa_wichuraiana Rw2G011440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 324
AccI GTMKAC 2 cut(s) 270, 885
AccII CGCG 3 cut(s) 74, 209, 507
AclI AACGTT 1 cut(s) 830
AclWI GGATC 1 cut(s) 475
AcoI YGGCCR 2 cut(s) 210, 432
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 5
AcyI GRCGYC 1 cut(s) 895
AfaI GTAC 2 cut(s) 443, 503
AfiI CCNNNNNNNGG 3 cut(s) 143, 451, 670
AgsI TTSAA 4 cut(s) 364, 692, 781, 836
AhdI GACNNNNNGTC 1 cut(s) 881
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 4 cut(s) 237, 450, 483, 783
AluBI AGCT 6 cut(s) 370, 550, 564, 613, 695, 790
AluI AGCT 6 cut(s) 370, 550, 564, 613, 695, 790
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 1 cut(s) 902
AlwI GGATC 1 cut(s) 475
Ama87I CYCGRG 2 cut(s) 371, 527
AoxI GGCC 5 cut(s) 40, 169, 210, 432, 853
ApaI GGGCCC 1 cut(s) 173
ApeKI GCWGC 5 cut(s) 34, 353, 556, 599, 922
ApoI RAATTY 1 cut(s) 731
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 6 cut(s) 61, 112, 169, 170, 448, 459
AsuC2I CCSGG 2 cut(s) 90, 446
AsuHPI GGTGA 5 cut(s) 16, 580, 652, 664, 773
AvaI CYCGRG 2 cut(s) 371, 527
AvaII GGWCC 4 cut(s) 61, 112, 448, 459
BaeGI GKGCMC 2 cut(s) 95, 173
BanII GRGCYC 4 cut(s) 173, 566, 579, 943
BbsI GAAGAC 1 cut(s) 939
Bbv12I GWGCWC 1 cut(s) 566
BbvI GCAGC 5 cut(s) 21, 340, 543, 586, 934
BccI CCATC 2 cut(s) 409, 731
BceAI ACGGC 1 cut(s) 424
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 4 cut(s) 239, 452, 485, 785
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 2 cut(s) 90, 446
BcoDI GTCTC 1 cut(s) 902
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 2 cut(s) 696, 920
BfoI RGCGCY 1 cut(s) 249
BisI GCNGC 7 cut(s) 35, 210, 298, 354, 557, 600, 923
BlsI GCNGC 7 cut(s) 36, 211, 299, 355, 558, 601, 924
Bme1390I CCNGG 6 cut(s) 90, 239, 446, 452, 485, 785
Bme18I GGWCC 4 cut(s) 61, 112, 448, 459
BmeRI GACNNNNNGTC 1 cut(s) 881
BmeT110I CYCGRG 2 cut(s) 371, 527
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 6 cut(s) 61, 112, 169, 170, 448, 459
BmiI GGNNCC 5 cut(s) 62, 113, 114, 171, 940
BmrFI CCNGG 6 cut(s) 90, 239, 446, 452, 485, 785
BpiI GAAGAC 1 cut(s) 939
BpuEI CTTGAG 3 cut(s) 563, 668, 884
BpuMI CCSGG 2 cut(s) 90, 446
BsaHI GRCGYC 1 cut(s) 895
BsaJI CCNNGG 4 cut(s) 183, 199, 213, 484
BsaWI WCCGGW 1 cut(s) 473
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 451, 670
BseBI CCWGG 4 cut(s) 239, 452, 485, 785
BseDI CCNNGG 4 cut(s) 183, 199, 213, 484
BseGI GGATG 3 cut(s) 195, 420, 723
BseLI CCNNNNNNNGG 3 cut(s) 143, 451, 670
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 2 cut(s) 95, 173
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 5 cut(s) 21, 340, 543, 586, 934
Bsh1236I CGCG 3 cut(s) 74, 209, 507
Bsh1285I CGRYCG 2 cut(s) 78, 213
BshFI GGCC 5 cut(s) 42, 171, 212, 434, 855
BsiEI CGRYCG 2 cut(s) 78, 213
BsiHKAI GWGCWC 1 cut(s) 566
BsiHKCI CYCGRG 2 cut(s) 371, 527
BsiSI CCGG 3 cut(s) 89, 446, 474
BslFI GGGAC 2 cut(s) 98, 260
BslI CCNNNNNNNGG 3 cut(s) 143, 451, 670
BsmAI GTCTC 1 cut(s) 902
BsmBI CGTCTC 1 cut(s) 902
BsmFI GGGAC 2 cut(s) 98, 260
BsnI GGCC 5 cut(s) 42, 171, 212, 434, 855
BsoBI CYCGRG 2 cut(s) 371, 527
Bsp120I GGGCCC 1 cut(s) 169
Bsp1286I GDGCHC 5 cut(s) 95, 173, 566, 579, 943
Bsp143I GATC 4 cut(s) 436, 480, 625, 769
BspANI GGCC 5 cut(s) 42, 171, 212, 434, 855
BspFNI CGCG 3 cut(s) 74, 209, 507
BspHI TCATGA 2 cut(s) 99, 516
BspLI GGNNCC 5 cut(s) 62, 113, 114, 171, 940
BspMAI CTGCAG 1 cut(s) 924
BspPI GGATC 1 cut(s) 475
BspQI GCTCTTC 1 cut(s) 307
BsrBI CCGCTC 1 cut(s) 324
BssECI CCNNGG 4 cut(s) 183, 199, 213, 484
BssMI GATC 4 cut(s) 436, 480, 625, 769
BssNI GRCGYC 1 cut(s) 895
Bst2UI CCWGG 4 cut(s) 239, 452, 485, 785
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 3 cut(s) 293, 307, 515
BstACI GRCGYC 1 cut(s) 895
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 5 cut(s) 156, 326, 432, 792, 853
BstDEI CTNAG 1 cut(s) 10
BstDSI CCRYGG 2 cut(s) 183, 199
BstF5I GGATG 3 cut(s) 195, 420, 723
BstFNI CGCG 3 cut(s) 74, 209, 507
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 4 cut(s) 439, 483, 628, 772
BstMAI GTCTC 1 cut(s) 902
BstMBI GATC 4 cut(s) 436, 480, 625, 769
BstMCI CGRYCG 2 cut(s) 78, 213
BstMWI GCNNNNNNNGC 3 cut(s) 31, 556, 901
BstNI CCWGG 4 cut(s) 239, 452, 485, 785
BstSCI CCNGG 6 cut(s) 88, 237, 444, 450, 483, 783
BstSFI CTRYAG 2 cut(s) 696, 920
BstSLI GKGCMC 2 cut(s) 95, 173
BstUI CGCG 3 cut(s) 74, 209, 507
BstV1I GCAGC 5 cut(s) 21, 340, 543, 586, 934
BstV2I GAAGAC 1 cut(s) 939
BstZI CGGCCG 1 cut(s) 210
BsuRI GGCC 5 cut(s) 42, 171, 212, 434, 855
BtgI CCRYGG 2 cut(s) 183, 199
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 3 cut(s) 195, 420, 723
Cac8I GCNNGC 5 cut(s) 156, 326, 432, 792, 853
CciI TCATGA 2 cut(s) 99, 516
CfoI GCGC 1 cut(s) 248
Cfr13I GGNCC 6 cut(s) 61, 112, 169, 170, 448, 459
CseI GACGC 2 cut(s) 518, 884
CsiI ACCWGGT 1 cut(s) 450
Csp6I GTAC 2 cut(s) 442, 502
CspCI CAANNNNNGTGG 2 cut(s) 165, 200
CviAII CATG 7 cut(s) 38, 100, 463, 490, 517, 802, 926
CviQI GTAC 2 cut(s) 442, 502
DdeI CTNAG 1 cut(s) 10
DpnI GATC 4 cut(s) 438, 482, 627, 771
DpnII GATC 4 cut(s) 436, 480, 625, 769
DriI GACNNNNNGTC 1 cut(s) 881
EaeI YGGCCR 2 cut(s) 210, 432
EagI CGGCCG 1 cut(s) 210
Eam1104I CTCTTC 3 cut(s) 293, 307, 515
Eam1105I GACNNNNNGTC 1 cut(s) 881
EarI CTCTTC 3 cut(s) 293, 307, 515
Ecl136II GAGCTC 1 cut(s) 564
EclXI CGGCCG 1 cut(s) 210
Eco24I GRGCYC 4 cut(s) 173, 566, 579, 943
Eco47I GGWCC 4 cut(s) 61, 112, 448, 459
Eco52I CGGCCG 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 5
Eco88I CYCGRG 2 cut(s) 371, 527
EcoICRI GAGCTC 1 cut(s) 564
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 4 cut(s) 237, 450, 483, 783
EcoT38I GRGCYC 4 cut(s) 173, 566, 579, 943
Esp3I CGTCTC 1 cut(s) 902
FaeI CATG 7 cut(s) 41, 103, 466, 493, 520, 805, 929
FaqI GGGAC 2 cut(s) 98, 260
FatI CATG 7 cut(s) 37, 99, 462, 489, 516, 801, 925
FauI CCCGC 3 cut(s) 49, 349, 404
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 2 cut(s) 270, 885
Fnu4HI GCNGC 7 cut(s) 35, 210, 298, 354, 557, 600, 923
FokI GGATG 3 cut(s) 182, 427, 710
FriOI GRGCYC 4 cut(s) 173, 566, 579, 943
Fsp4HI GCNGC 7 cut(s) 35, 210, 298, 354, 557, 600, 923
FspBI CTAG 1 cut(s) 815
GlaI GCGC 1 cut(s) 247
GluI GCNGC 7 cut(s) 35, 210, 298, 354, 557, 600, 923
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 5 cut(s) 42, 171, 212, 434, 855
HapII CCGG 3 cut(s) 89, 446, 474
HgaI GACGC 2 cut(s) 518, 884
HhaI GCGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 895
Hin1II CATG 7 cut(s) 41, 103, 466, 493, 520, 805, 929
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HinfI GANTC 1 cut(s) 493
HpaII CCGG 3 cut(s) 89, 446, 474
HphI GGTGA 5 cut(s) 16, 580, 652, 664, 773
Hpy166II GTNNAC 4 cut(s) 7, 271, 660, 886
Hpy188I TCNGA 4 cut(s) 65, 547, 682, 774
Hpy188III TCNNGA 4 cut(s) 100, 115, 517, 623
Hpy8I GTNNAC 4 cut(s) 7, 271, 660, 886
Hpy99I CGWCG 4 cut(s) 334, 512, 544, 547
HpyAV CCTTC 5 cut(s) 136, 565, 664, 830, 841
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 4 cut(s) 227, 273, 332, 830
HpyCH4V TGCA 3 cut(s) 37, 827, 922
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 556, 901
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 4 cut(s) 227, 273, 332, 830
Hsp92I GRCGYC 1 cut(s) 895
Hsp92II CATG 7 cut(s) 41, 103, 466, 493, 520, 805, 929
HspAI GCGC 1 cut(s) 246
KflI GGGWCCC 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 4 cut(s) 436, 480, 625, 769
LguI GCTCTTC 1 cut(s) 307
LmnI GCTCC 4 cut(s) 547, 787, 818, 938
Lsp1109I GCAGC 5 cut(s) 21, 340, 543, 586, 934
MabI ACCWGGT 1 cut(s) 450
MaeI CTAG 1 cut(s) 815
MaeII ACGT 4 cut(s) 227, 273, 332, 830
MaeIII GTNAC 2 cut(s) 223, 404
MalI GATC 4 cut(s) 438, 482, 627, 771
MbiI CCGCTC 1 cut(s) 324
MboI GATC 4 cut(s) 436, 480, 625, 769
MboII GAAGA 7 cut(s) 280, 283, 294, 376, 532, 941, 944
MhlI GDGCHC 5 cut(s) 95, 173, 566, 579, 943
MluCI AATT 1 cut(s) 731
MlyI GAGTC 1 cut(s) 487
MmeI TCCRAC 2 cut(s) 88, 525
MnlI CCTC 7 cut(s) 137, 208, 293, 296, 516, 643, 669
MslI CAYNNNNRTG 2 cut(s) 98, 467
MspI CCGG 3 cut(s) 89, 446, 474
MspR9I CCNGG 6 cut(s) 90, 239, 446, 452, 485, 785
MvaI CCWGG 4 cut(s) 239, 452, 485, 785
MvnI CGCG 3 cut(s) 74, 209, 507
MwoI GCNNNNNNNGC 3 cut(s) 31, 556, 901
NciI CCSGG 2 cut(s) 90, 446
NdeII GATC 4 cut(s) 436, 480, 625, 769
NlaIII CATG 7 cut(s) 41, 103, 466, 493, 520, 805, 929
NlaIV GGNNCC 5 cut(s) 62, 113, 114, 171, 940
NmeAIII GCCGAG 2 cut(s) 238, 585
NmuCI GTSAC 1 cut(s) 223
PagI TCATGA 2 cut(s) 99, 516
PciSI GCTCTTC 1 cut(s) 307
PkrI GCNGC 7 cut(s) 36, 211, 299, 355, 558, 601, 924
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 566
Psp1406I AACGTT 1 cut(s) 830
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 4 cut(s) 237, 450, 483, 783
PspGI CCWGG 4 cut(s) 237, 450, 483, 783
PspN4I GGNNCC 5 cut(s) 62, 113, 114, 171, 940
PspOMI GGGCCC 1 cut(s) 169
PspPI GGNCC 6 cut(s) 61, 112, 169, 170, 448, 459
PspPPI RGGWCCY 1 cut(s) 112
PstI CTGCAG 1 cut(s) 924
RsaI GTAC 2 cut(s) 443, 503
RsaNI GTAC 2 cut(s) 442, 502
RseI CAYNNNNRTG 2 cut(s) 98, 467
SacI GAGCTC 1 cut(s) 566
SapI GCTCTTC 1 cut(s) 307
SatI GCNGC 7 cut(s) 35, 210, 298, 354, 557, 600, 923
Sau3AI GATC 4 cut(s) 436, 480, 625, 769
Sau96I GGNCC 6 cut(s) 61, 112, 169, 170, 448, 459
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 6 cut(s) 90, 239, 446, 452, 485, 785
SduI GDGCHC 5 cut(s) 95, 173, 566, 579, 943
SexAI ACCWGGT 1 cut(s) 450
SfcI CTRYAG 2 cut(s) 696, 920
SinI GGWCC 4 cut(s) 61, 112, 448, 459
SmiMI CAYNNNNRTG 2 cut(s) 98, 467
SmlI CTYRAG 3 cut(s) 578, 647, 899
SmoI CTYRAG 3 cut(s) 578, 647, 899
Sse9I AATT 1 cut(s) 731
SspMI CTAG 1 cut(s) 815
SstI GAGCTC 1 cut(s) 566
StyD4I CCNGG 6 cut(s) 88, 237, 444, 450, 483, 783
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 4 cut(s) 230, 276, 335, 833
TaqI TCGA 3 cut(s) 78, 84, 479
TasI AATT 1 cut(s) 731
TauI GCSGC 2 cut(s) 212, 300
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 5 cut(s) 34, 353, 556, 599, 922
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 6 cut(s) 62, 116, 533, 818, 854, 942
TspGWI ACGGA 2 cut(s) 256, 951
VpaK11BI GGWCC 4 cut(s) 61, 112, 448, 459
XapI RAATTY 1 cut(s) 731
XmiI GTMKAC 2 cut(s) 270, 885
XspI CTAG 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.