Rroxscaffold_2G00141330

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011037
Physical Location & Seq
Forward (+)
79644142 .. 79646476
2335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141330.1

Sequence Viewer

Length: 945 bp
ATGGTGAACTTAGTAGCACTACAGAAGCCCTTGTTGCATGGCCTAATGAAAATGGCCGGAGTGAAACCCTACACGGTGGAGATCGAGCCCGGCACAGTGATGAACTTCTGGGTCCCGAAAGAAACCCTAAAGAAGCAGAAGAAGGGCGAGAAGCCCGAGATCATATCCAAACCCAACAAGCCGGTCGTGGTTCTGATACACGGCTTTGCCGCCGAGGGAATAGTGACTTGGCAGTTCCAGGTGGGCGCGTTGTCCAAGAAATACTCTGTCTACGTTCCGGACCTCCTGTTTTTCGGTGGGTCGACCACCGACAAGCCCGACAGGTCCCCGGCTCTCCAGGCCGAGTGCGTCTCTACGGGGCTGCTGAAGCTCGGTGTGGACACTTGTACGGTGGTGGGATTCAGTTACGGCGGCATGGTGGCGTTCAAGATGGCGGAGCTGTATCCGGAGCTGGTGCGGGTCATGGTGGTTTCCGGCTCAATATTGGCGATGACGTGTTCGGTAAGCGAGGAGACTATGAAGAGGCTAGGGTTCGAGTCGTCGTCGGAGCTTTTGCTGCCGAGCACGGTGAAGGGGCTCAAGGCTCTGCTCTCGGTGGCAGCTCACAAGAAGCTTTATTTTCCCGATCGGCTCCACAAAGACTTCCTTGAGGTGATGTTCACCAACAGGAAGGAGAGAGCAGAACTCCTTGAAGCTTTAGTCATTGACACAAAGGATCCAAACATTCCCAAATTCCCTCAGAGAATACACCTCTTATGGGGTGAAAATGATCAGATATTTAAGCAGGAGCTTGCTCACAACATGAAAGAGCAAGTAGGAGCAAATGCAACATTTGAAGGCATAAAGAAGGCAGGTCACTTGGTTCACTTGGAAAGACCTTGTGTTTACAATAGGTATCTCAAGAGATTCCTTGCTTCCCTGAATGAAGTTGGAGATCAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

314

Amino Acids

35.05

Weight (kDa)

9.06

Isoelectric Point (pI)

29.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 61 - 175 1.4e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 63 - 294 1e-15 Alpha/beta hydrolase family
Hydrolase_4 PF12146 127 - 292 9.3e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18360 AT2G18360 AT4G36610
fragaria_vesca FvH4_1g13190 FvH4_1g13200
malus_domestica MD02G1146700.v1.1 MD15G1260700.v1.1
prunus_persica Prupe.7G155500_v2.0.a1
pyrus_communis pycom02g11600 pycom15g22790
rosa_chinensis RchiOBHm_Chr2g0101411
rosa_laevigata RLG00000017076
rosa_multiflora Rmu_sc0006128.1_g000006
rosa_roxburghii Rroxscaffold_2G00141330
rosa_rugosa Rorug02G0098400
rosa_samantha Rh2AG146700 Rh2BG152100 Rh2CG152600 Rh2DG152200
rosa_wichuraiana Rw2G011440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 842
AccI GTMKAC 2 cut(s) 270, 302
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 2 cut(s) 277, 445
AciI CCGC 4 cut(s) 210, 411, 434, 457
AclWI GGATC 2 cut(s) 710, 723
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 386
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 757
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 3 cut(s) 427, 692, 836
AjiI CACGTC 1 cut(s) 495
AjnI CCWGG 2 cut(s) 237, 336
AjuI GAANNNNNNNTTGG 2 cut(s) 211, 243
AluBI AGCT 8 cut(s) 370, 439, 451, 550, 602, 613, 695, 790
AluI AGCT 8 cut(s) 370, 439, 451, 550, 602, 613, 695, 790
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 2 cut(s) 355, 506
AlwI GGATC 2 cut(s) 710, 723
Ama87I CYCGRG 1 cut(s) 155
Aor13HI TCCGGA 2 cut(s) 277, 445
AoxI GGCC 3 cut(s) 40, 54, 339
ApeKI GCWGC 3 cut(s) 361, 556, 599
ApoI RAATTY 1 cut(s) 731
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 3 cut(s) 112, 280, 324
AsuC2I CCSGG 2 cut(s) 90, 329
AsuHPI GGTGA 5 cut(s) 16, 580, 652, 664, 773
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 3 cut(s) 112, 280, 324
BamHI GGATCC 1 cut(s) 715
BanII GRGCYC 2 cut(s) 90, 579
Bbv12I GWGCWC 1 cut(s) 566
BbvI GCAGC 3 cut(s) 348, 543, 611
BccI CCATC 1 cut(s) 424
BceAI ACGGC 2 cut(s) 217, 424
BciT130I CCWGG 2 cut(s) 239, 338
BciVI GTATCC 1 cut(s) 453
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 2 cut(s) 90, 329
BcoDI GTCTC 2 cut(s) 355, 506
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 842
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 5 cut(s) 210, 362, 412, 557, 600
BlsI GCNGC 5 cut(s) 211, 363, 413, 558, 601
Bme1390I CCNGG 4 cut(s) 90, 239, 329, 338
Bme18I GGWCC 3 cut(s) 112, 280, 324
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 1 cut(s) 495
BmgT120I GGNCC 3 cut(s) 112, 280, 324
BmiI GGNNCC 5 cut(s) 113, 114, 326, 632, 717
BmrFI CCNGG 4 cut(s) 90, 239, 329, 338
BplI GAGNNNNNCTC 4 cut(s) 335, 367, 669, 701
BpmI CTGGAG 1 cut(s) 320
BpuEI CTTGAG 3 cut(s) 563, 668, 884
BpuMI CCSGG 2 cut(s) 90, 329
BsaJI CCNNGG 2 cut(s) 213, 327
BsaWI WCCGGW 2 cut(s) 277, 445
Bsc4I CCNNNNNNNGG 1 cut(s) 757
Bse118I RCCGGY 1 cut(s) 181
BseAI TCCGGA 2 cut(s) 277, 445
BseBI CCWGG 2 cut(s) 239, 338
BseDI CCNNGG 2 cut(s) 213, 327
BseLI CCNNNNNNNGG 1 cut(s) 757
BseMII CTCAG 1 cut(s) 752
BseRI GAGGAG 1 cut(s) 524
BseXI GCAGC 3 cut(s) 348, 543, 611
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 2 cut(s) 186, 628
BshFI GGCC 3 cut(s) 42, 56, 341
BsiEI CGRYCG 2 cut(s) 186, 628
BsiHKAI GWGCWC 1 cut(s) 566
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 7 cut(s) 57, 90, 182, 278, 329, 446, 474
BslFI GGGAC 2 cut(s) 98, 310
BslI CCNNNNNNNGG 1 cut(s) 757
BsmAI GTCTC 2 cut(s) 355, 506
BsmBI CGTCTC 1 cut(s) 355
BsmFI GGGAC 2 cut(s) 98, 310
BsnI GGCC 3 cut(s) 42, 56, 341
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 3 cut(s) 90, 566, 579
Bsp13I TCCGGA 2 cut(s) 277, 445
Bsp143I GATC 6 cut(s) 81, 159, 625, 715, 769, 934
BspACI CCGC 4 cut(s) 210, 411, 434, 457
BspANI GGCC 3 cut(s) 42, 56, 341
BspCNI CTCAG 1 cut(s) 751
BspEI TCCGGA 2 cut(s) 277, 445
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 5 cut(s) 113, 114, 326, 632, 717
BspMI ACCTGC 1 cut(s) 842
BspPI GGATC 2 cut(s) 710, 723
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 213, 327
BssMI GATC 6 cut(s) 81, 159, 625, 715, 769, 934
Bst2UI CCWGG 2 cut(s) 239, 338
Bst4CI ACNGT 4 cut(s) 76, 97, 391, 568
Bst6I CTCTTC 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 792
BstDEI CTNAG 2 cut(s) 10, 738
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 6 cut(s) 84, 162, 628, 718, 772, 937
BstMAI GTCTC 2 cut(s) 355, 506
BstMBI GATC 6 cut(s) 81, 159, 625, 715, 769, 934
BstMCI CGRYCG 2 cut(s) 186, 628
BstMWI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
BstNI CCWGG 2 cut(s) 239, 338
BstSCI CCNGG 4 cut(s) 88, 237, 327, 336
BstSFI CTRYAG 1 cut(s) 20
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 348, 543, 611
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 3 cut(s) 42, 56, 341
BtgZI GCGATG 1 cut(s) 503
BtrI CACGTC 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 842
Cac8I GCNNGC 1 cut(s) 792
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 3 cut(s) 112, 280, 324
CseI GACGC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 387
CviAII CATG 4 cut(s) 38, 415, 463, 802
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 2 cut(s) 10, 738
DpnI GATC 6 cut(s) 83, 161, 627, 717, 771, 936
DpnII GATC 6 cut(s) 81, 159, 625, 715, 769, 934
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
EciI GGCGGA 1 cut(s) 449
Eco24I GRGCYC 2 cut(s) 90, 579
Eco47I GGWCC 3 cut(s) 112, 280, 324
Eco57I CTGAAG 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 155
EcoO109I RGGNCCY 2 cut(s) 112, 324
EcoRII CCWGG 2 cut(s) 237, 336
EcoT38I GRGCYC 2 cut(s) 90, 579
Esp3I CGTCTC 1 cut(s) 355
FaeI CATG 4 cut(s) 41, 418, 466, 805
FaiI YATR 8 cut(s) 39, 164, 416, 464, 518, 757, 803, 842
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 2 cut(s) 98, 310
FatI CATG 4 cut(s) 37, 414, 462, 801
FauI CCCGC 1 cut(s) 450
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 2 cut(s) 270, 302
Fnu4HI GCNGC 5 cut(s) 210, 362, 412, 557, 600
FriOI GRGCYC 2 cut(s) 90, 579
Fsp4HI GCNGC 5 cut(s) 210, 362, 412, 557, 600
FspBI CTAG 1 cut(s) 527
GlaI GCGC 1 cut(s) 247
GluI GCNGC 5 cut(s) 210, 362, 412, 557, 600
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 3 cut(s) 42, 56, 341
HapII CCGG 7 cut(s) 57, 90, 182, 278, 329, 446, 474
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 248
Hin1II CATG 4 cut(s) 41, 418, 466, 805
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HindIII AAGCTT 2 cut(s) 611, 693
HinfI GANTC 3 cut(s) 399, 536, 906
HpaII CCGG 7 cut(s) 57, 90, 182, 278, 329, 446, 474
HphI GGTGA 5 cut(s) 16, 580, 652, 664, 773
Hpy166II GTNNAC 7 cut(s) 7, 271, 303, 379, 660, 865, 886
Hpy188I TCNGA 4 cut(s) 195, 547, 741, 774
Hpy188III TCNNGA 6 cut(s) 115, 278, 427, 446, 623, 901
Hpy8I GTNNAC 7 cut(s) 7, 271, 303, 379, 660, 865, 886
Hpy99I CGWCG 2 cut(s) 544, 547
HpyAV CCTTC 5 cut(s) 136, 565, 664, 830, 841
HpyCH4III ACNGT 4 cut(s) 76, 97, 391, 568
HpyCH4IV ACGT 2 cut(s) 273, 494
HpyCH4V TGCA 2 cut(s) 37, 827
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
HpyF3I CTNAG 2 cut(s) 10, 738
HpySE526I ACGT 2 cut(s) 273, 494
Hsp92II CATG 4 cut(s) 41, 418, 466, 805
HspAI GCGC 1 cut(s) 246
KflI GGGWCCC 1 cut(s) 112
Kpn2I TCCGGA 2 cut(s) 277, 445
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 6 cut(s) 81, 159, 625, 715, 769, 934
LmnI GCTCC 6 cut(s) 436, 448, 547, 636, 787, 818
Lsp1109I GCAGC 3 cut(s) 348, 543, 611
MaeI CTAG 1 cut(s) 527
MaeII ACGT 2 cut(s) 273, 494
MaeIII GTNAC 3 cut(s) 223, 404, 854
MalI GATC 6 cut(s) 83, 161, 627, 717, 771, 936
MboI GATC 6 cut(s) 81, 159, 625, 715, 769, 934
MboII GAAGA 2 cut(s) 151, 532
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 3 cut(s) 90, 566, 579
MluCI AATT 2 cut(s) 731, 940
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 2 cut(s) 525, 910
MnlI CCTC 7 cut(s) 208, 293, 502, 516, 643, 747, 761
MroI TCCGGA 2 cut(s) 277, 445
MseI TTAA 2 cut(s) 780, 943
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 7 cut(s) 57, 90, 182, 278, 329, 446, 474
MspR9I CCNGG 4 cut(s) 90, 239, 329, 338
MvaI CCWGG 2 cut(s) 239, 338
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
NciI CCSGG 2 cut(s) 90, 329
NdeII GATC 6 cut(s) 81, 159, 625, 715, 769, 934
NlaIII CATG 4 cut(s) 41, 418, 466, 805
NlaIV GGNNCC 5 cut(s) 113, 114, 326, 632, 717
NmeAIII GCCGAG 3 cut(s) 238, 367, 585
NmuCI GTSAC 2 cut(s) 223, 854
PfeI GAWTC 2 cut(s) 399, 906
PkrI GCNGC 5 cut(s) 211, 363, 413, 558, 601
Ple19I CGATCG 1 cut(s) 628
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PpuMI RGGWCCY 2 cut(s) 112, 324
Psp5II RGGWCCY 2 cut(s) 112, 324
Psp6I CCWGG 2 cut(s) 237, 336
PspGI CCWGG 2 cut(s) 237, 336
PspN4I GGNNCC 5 cut(s) 113, 114, 326, 632, 717
PspPI GGNCC 3 cut(s) 112, 280, 324
PspPPI RGGWCCY 2 cut(s) 112, 324
PsuI RGATCY 1 cut(s) 715
PvuI CGATCG 1 cut(s) 628
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
RseI CAYNNNNRTG 1 cut(s) 98
SalI GTCGAC 1 cut(s) 301
SaqAI TTAA 2 cut(s) 780, 943
SatI GCNGC 5 cut(s) 210, 362, 412, 557, 600
Sau3AI GATC 6 cut(s) 81, 159, 625, 715, 769, 934
Sau96I GGNCC 3 cut(s) 112, 280, 324
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 4 cut(s) 90, 239, 329, 338
SduI GDGCHC 3 cut(s) 90, 566, 579
SfcI CTRYAG 1 cut(s) 20
SinI GGWCC 3 cut(s) 112, 280, 324
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 578, 647, 899
SmoI CTYRAG 3 cut(s) 578, 647, 899
Sse9I AATT 2 cut(s) 731, 940
SsiI CCGC 4 cut(s) 210, 411, 434, 457
SspI AATATT 1 cut(s) 483
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 4 cut(s) 88, 237, 327, 336
TaaI ACNGT 4 cut(s) 76, 97, 391, 568
TaiI ACGT 2 cut(s) 276, 497
TaqI TCGA 3 cut(s) 84, 302, 534
TasI AATT 2 cut(s) 731, 940
TauI GCSGC 2 cut(s) 212, 414
TfiI GAWTC 2 cut(s) 399, 906
Tru1I TTAA 2 cut(s) 780, 943
Tru9I TTAA 2 cut(s) 780, 943
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 2 cut(s) 223, 854
TseI GCWGC 3 cut(s) 361, 556, 599
Tsp45I GTSAC 2 cut(s) 223, 854
TspDTI ATGAA 5 cut(s) 62, 116, 533, 818, 939
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 3 cut(s) 112, 280, 324
XapI RAATTY 1 cut(s) 731
XmiI GTMKAC 2 cut(s) 270, 302
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.