Rh2DG152200

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011037
Physical Location & Seq
Reverse (-)
12723241 .. 12726860
3620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG152200.1

Sequence Viewer

Length: 669 bp
ATGGTGAACTTAGTAGCACTACAGAAGCCCTTGTTGCATGGCCTAATGAAAATGGCCGGAGTGAAACCCTACACGGTGGAGATCGAGCCCGGCACAGTGATGAACTTCTGGGTCCCGAAAGAAACCCTAAAGAAGCCGAAGAAGGGCGAGAAGCCCGAGATCATATCCAAACCCAACAAGCCGGTCGTGGTTCTGATACACGGCTTCGCCGCCGAGGGAATAGTGACGTGGCAATTCCAGGTGGGCGCGTTGTCCAAGAAATACTCGGTCTACGTTCCCGACCTCCTGTTTTTCGGTGGGTCGACCACCGACAAGCCCGACAGGTCCCCGGCTCTCCAGGCCGAGTGCGTCTCTACGGGGCTGAGGAAGCTCGGTGTGGACACGTGTACGGTGGTGGGATTCAGTTACGGCGGCATGGTGGCCTTCAAGATGGCGGAGCTGTATCCGGAGCTGGTGCGGGTCATGGTGGTTTCTGGCTCAATATTGGCGATGACGTGTTCGGTGAGCGAGGAGACTATGAAGAGGCTAGGGTTCGAGTCGTCGTCGGAGCTTTTGCTGCCGAGCACGGTGAAGGGGCTCAAGGCTCTGCTCTCGGTGGCAGCTCACAAGAAGCTTTATTTTCCCGATCGGCTCCACAAAGACTTCCTTGAGGTTAGTGAACAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

222

Amino Acids

24.4

Weight (kDa)

9.11

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 61 - 175 3.2e-18 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 63 - 178 4.5e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18360 AT2G18360 AT4G36610
fragaria_vesca FvH4_1g13190 FvH4_1g13200
malus_domestica MD02G1146700.v1.1 MD15G1260700.v1.1
prunus_persica Prupe.7G155500_v2.0.a1
pyrus_communis pycom02g11600 pycom15g22790
rosa_chinensis RchiOBHm_Chr2g0101411
rosa_laevigata RLG00000017076
rosa_multiflora Rmu_sc0006128.1_g000006
rosa_roxburghii Rroxscaffold_2G00141330
rosa_rugosa Rorug02G0098400
rosa_samantha Rh2AG146700 Rh2BG152100 Rh2CG152600 Rh2DG152200
rosa_wichuraiana Rw2G011440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 270, 302
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 4 cut(s) 210, 411, 434, 457
AcoI YGGCCR 1 cut(s) 54
AcvI CACGTG 1 cut(s) 384
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 143
AflIII ACRYGT 3 cut(s) 381, 383, 494
AgsI TTSAA 1 cut(s) 427
AjiI CACGTC 2 cut(s) 228, 495
AjnI CCWGG 2 cut(s) 237, 336
AluBI AGCT 6 cut(s) 370, 439, 451, 550, 602, 613
AluI AGCT 6 cut(s) 370, 439, 451, 550, 602, 613
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 2 cut(s) 355, 506
Ama87I CYCGRG 1 cut(s) 155
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 4 cut(s) 40, 54, 339, 420
ApeKI GCWGC 2 cut(s) 556, 599
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 2 cut(s) 112, 324
AsuC2I CCSGG 2 cut(s) 90, 329
AsuHPI GGTGA 3 cut(s) 16, 514, 580
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 2 cut(s) 112, 324
BanII GRGCYC 2 cut(s) 90, 579
BbrPI CACGTG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 566
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 2 cut(s) 543, 611
BccI CCATC 1 cut(s) 424
BceAI ACGGC 2 cut(s) 217, 424
BciT130I CCWGG 2 cut(s) 239, 338
BciVI GTATCC 1 cut(s) 453
BcnI CCSGG 2 cut(s) 90, 329
BcoDI GTCTC 2 cut(s) 355, 506
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 20
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 4 cut(s) 210, 412, 557, 600
BlsI GCNGC 4 cut(s) 211, 413, 558, 601
Bme1390I CCNGG 4 cut(s) 90, 239, 329, 338
Bme18I GGWCC 2 cut(s) 112, 324
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 2 cut(s) 228, 495
BmgT120I GGNCC 2 cut(s) 112, 324
BmiI GGNNCC 4 cut(s) 113, 114, 326, 632
BmrFI CCNGG 4 cut(s) 90, 239, 329, 338
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 362
BpuEI CTTGAG 2 cut(s) 563, 668
BpuMI CCSGG 2 cut(s) 90, 329
BsaAI YACGTR 1 cut(s) 384
BsaJI CCNNGG 2 cut(s) 213, 327
BsaWI WCCGGW 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse118I RCCGGY 1 cut(s) 181
BseAI TCCGGA 1 cut(s) 445
BseBI CCWGG 2 cut(s) 239, 338
BseDI CCNNGG 2 cut(s) 213, 327
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMII CTCAG 1 cut(s) 353
BseRI GAGGAG 1 cut(s) 524
BseXI GCAGC 2 cut(s) 543, 611
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 2 cut(s) 186, 628
BshFI GGCC 4 cut(s) 42, 56, 341, 422
BsiEI CGRYCG 2 cut(s) 186, 628
BsiHKAI GWGCWC 1 cut(s) 566
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 5 cut(s) 57, 90, 182, 329, 446
BslFI GGGAC 2 cut(s) 98, 310
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 355, 506
BsmBI CGTCTC 1 cut(s) 355
BsmFI GGGAC 2 cut(s) 98, 310
BsnI GGCC 4 cut(s) 42, 56, 341, 422
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 3 cut(s) 90, 566, 579
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 3 cut(s) 81, 159, 625
BspACI CCGC 4 cut(s) 210, 411, 434, 457
BspANI GGCC 4 cut(s) 42, 56, 341, 422
BspCNI CTCAG 1 cut(s) 354
BspEI TCCGGA 1 cut(s) 445
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 4 cut(s) 113, 114, 326, 632
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 213, 327
BssMI GATC 3 cut(s) 81, 159, 625
Bst2UI CCWGG 2 cut(s) 239, 338
Bst4CI ACNGT 4 cut(s) 76, 97, 391, 568
Bst6I CTCTTC 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 10, 362
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 3 cut(s) 84, 162, 628
BstMAI GTCTC 2 cut(s) 355, 506
BstMBI GATC 3 cut(s) 81, 159, 625
BstMCI CGRYCG 2 cut(s) 186, 628
BstMWI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
BstNI CCWGG 2 cut(s) 239, 338
BstSCI CCNGG 4 cut(s) 88, 237, 327, 336
BstSFI CTRYAG 1 cut(s) 20
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 543, 611
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 4 cut(s) 42, 56, 341, 422
BtgZI GCGATG 1 cut(s) 503
BtrI CACGTC 2 cut(s) 228, 495
BtsIMutI CAGTG 1 cut(s) 102
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 112, 324
CseI GACGC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 387
CviAII CATG 3 cut(s) 38, 415, 463
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 2 cut(s) 10, 362
DpnI GATC 3 cut(s) 83, 161, 627
DpnII GATC 3 cut(s) 81, 159, 625
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
EciI GGCGGA 1 cut(s) 449
Eco24I GRGCYC 2 cut(s) 90, 579
Eco47I GGWCC 2 cut(s) 112, 324
Eco72I CACGTG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 155
EcoO109I RGGNCCY 2 cut(s) 112, 324
EcoRII CCWGG 2 cut(s) 237, 336
EcoT38I GRGCYC 2 cut(s) 90, 579
Esp3I CGTCTC 1 cut(s) 355
FaeI CATG 3 cut(s) 41, 418, 466
FaiI YATR 5 cut(s) 39, 164, 416, 464, 518
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 2 cut(s) 98, 310
FatI CATG 3 cut(s) 37, 414, 462
FauI CCCGC 1 cut(s) 450
FblI GTMKAC 2 cut(s) 270, 302
Fnu4HI GCNGC 4 cut(s) 210, 412, 557, 600
FriOI GRGCYC 2 cut(s) 90, 579
Fsp4HI GCNGC 4 cut(s) 210, 412, 557, 600
FspBI CTAG 1 cut(s) 527
GlaI GCGC 1 cut(s) 247
GluI GCNGC 4 cut(s) 210, 412, 557, 600
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 4 cut(s) 42, 56, 341, 422
HapII CCGG 5 cut(s) 57, 90, 182, 329, 446
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 248
Hin1II CATG 3 cut(s) 41, 418, 466
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 2 cut(s) 399, 536
HpaII CCGG 5 cut(s) 57, 90, 182, 329, 446
HphI GGTGA 3 cut(s) 16, 514, 580
Hpy166II GTNNAC 6 cut(s) 7, 271, 303, 379, 387, 659
Hpy188I TCNGA 2 cut(s) 195, 547
Hpy188III TCNNGA 5 cut(s) 115, 278, 427, 446, 623
Hpy8I GTNNAC 6 cut(s) 7, 271, 303, 379, 387, 659
Hpy99I CGWCG 2 cut(s) 544, 547
HpyAV CCTTC 3 cut(s) 136, 433, 565
HpyCH4III ACNGT 4 cut(s) 76, 97, 391, 568
HpyCH4IV ACGT 4 cut(s) 227, 273, 383, 494
HpyCH4V TGCA 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
HpyF3I CTNAG 2 cut(s) 10, 362
HpySE526I ACGT 4 cut(s) 227, 273, 383, 494
Hsp92II CATG 3 cut(s) 41, 418, 466
HspAI GCGC 1 cut(s) 246
KflI GGGWCCC 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 445
Kzo9I GATC 3 cut(s) 81, 159, 625
LmnI GCTCC 4 cut(s) 436, 448, 547, 636
Lsp1109I GCAGC 2 cut(s) 543, 611
MaeI CTAG 1 cut(s) 527
MaeII ACGT 4 cut(s) 227, 273, 383, 494
MaeIII GTNAC 2 cut(s) 223, 404
MalI GATC 3 cut(s) 83, 161, 627
MboI GATC 3 cut(s) 81, 159, 625
MboII GAAGA 2 cut(s) 151, 532
MhlI GDGCHC 3 cut(s) 90, 566, 579
MluCI AATT 1 cut(s) 233
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 6 cut(s) 208, 293, 357, 502, 516, 643
MroI TCCGGA 1 cut(s) 445
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 5 cut(s) 57, 90, 182, 329, 446
MspR9I CCNGG 4 cut(s) 90, 239, 329, 338
MvaI CCWGG 2 cut(s) 239, 338
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 34, 338, 367, 556
NciI CCSGG 2 cut(s) 90, 329
NdeII GATC 3 cut(s) 81, 159, 625
NlaIII CATG 3 cut(s) 41, 418, 466
NlaIV GGNNCC 4 cut(s) 113, 114, 326, 632
NmeAIII GCCGAG 3 cut(s) 238, 367, 585
NmuCI GTSAC 1 cut(s) 223
PfeI GAWTC 1 cut(s) 399
PkrI GCNGC 4 cut(s) 211, 413, 558, 601
Ple19I CGATCG 1 cut(s) 628
PleI GAGTC 1 cut(s) 544
PmaCI CACGTG 1 cut(s) 384
PmlI CACGTG 1 cut(s) 384
PpsI GAGTC 1 cut(s) 544
Ppu21I YACGTR 1 cut(s) 384
PpuMI RGGWCCY 2 cut(s) 112, 324
Psp5II RGGWCCY 2 cut(s) 112, 324
Psp6I CCWGG 2 cut(s) 237, 336
PspCI CACGTG 1 cut(s) 384
PspGI CCWGG 2 cut(s) 237, 336
PspN4I GGNNCC 4 cut(s) 113, 114, 326, 632
PspPI GGNCC 2 cut(s) 112, 324
PspPPI RGGWCCY 2 cut(s) 112, 324
PvuI CGATCG 1 cut(s) 628
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
RseI CAYNNNNRTG 1 cut(s) 98
SalI GTCGAC 1 cut(s) 301
SatI GCNGC 4 cut(s) 210, 412, 557, 600
Sau3AI GATC 3 cut(s) 81, 159, 625
Sau96I GGNCC 2 cut(s) 112, 324
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 4 cut(s) 90, 239, 329, 338
SduI GDGCHC 3 cut(s) 90, 566, 579
SfcI CTRYAG 1 cut(s) 20
SinI GGWCC 2 cut(s) 112, 324
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 578, 647
SmoI CTYRAG 2 cut(s) 578, 647
Sse9I AATT 1 cut(s) 233
SsiI CCGC 4 cut(s) 210, 411, 434, 457
SspI AATATT 1 cut(s) 483
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 4 cut(s) 88, 237, 327, 336
TaaI ACNGT 4 cut(s) 76, 97, 391, 568
TaiI ACGT 4 cut(s) 230, 276, 386, 497
TaqI TCGA 3 cut(s) 84, 302, 534
TaqII GACCGA 1 cut(s) 256
TasI AATT 1 cut(s) 233
TauI GCSGC 2 cut(s) 212, 414
TfiI GAWTC 1 cut(s) 399
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 556, 599
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 3 cut(s) 62, 116, 533
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 112, 324
XmiI GTMKAC 2 cut(s) 270, 302
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.