FvH4_1g14181

Cold acclimation protein WCOR413

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013591
Physical Location & Seq
Forward (+)
7850083 .. 7851452
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14181.t1

Sequence Viewer

Length: 522 bp
ATGGGTTTTGCTCAATTCATGGAGTTTGAAGAAGGAGGAGTTGGATCCAGTGGAGCTCTTAATAGCTTTCAATGGGGTGGAACCATCTTTGCCTTAATTTTGTTAATCCTGAATCGAGTAGGGCGCAGATCAGGCATGCAAAGTAACCTTCTTGTAATGTATTTGTTTACAAGCTTCCCGACGGTGTTGTTTAAAATTCTGAGGGGACAGTTCGGCTATTGGGTTTCATTTCTTGCTGTTTCTGCAAACCTCTTCTTTCCACAAACTTTTCCAGTTTCTCGTTTTCTCCTATTTGTGGTTACACCTACTTGGGTGGCTAATGGACTGCGTAATAGTATTGTGGGTGGCATCTTTTGTCTAATTATTGGAGTTCTAGTTGTCATAACAGCAATTCAAGGAATTGGAGGCTTTAACGATTGTTTTAGTAACTGTCAATGCAATTGTCATTGCTTTGTGTACTGTCTTAGTATAGGATTTATCTTCTTCTTTACCATTTTGTATCTGTGCTCAGAAACTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WCOR413

Protein Analysis

174

Amino Acids

19.25

Weight (kDa)

8.16

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WCOR413 PF05562 20 - 144 3e-29 Cold acclimation protein WCOR413
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013591)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14181 FvH4_1g14252
malus_domestica MD15G1270400.v1.1
prunus_persica Prupe.7G147700_v2.0.a1
pyrus_communis pycom15g23460
rosa_chinensis RchiOBHm_Chr2g0102851
rosa_laevigata RLG00000017184
rosa_multiflora Rmu_sc0000388.1_g000049
rosa_roxburghii Rroxscaffold_2G00140250
rosa_rugosa Rorug02G0110100
rosa_samantha Rh2AG158500 Rh2AG434100 Rh2BG164900 Rh2CG164600 Rh2CG419800 Rh2DG163700
rosa_wichuraiana Rw2G012330 Rw2G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 39, 52
AcsI RAATTY 1 cut(s) 195
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 1 cut(s) 295
AgsI TTSAA 3 cut(s) 29, 71, 395
AjuI GAANNNNNNNTTGG 2 cut(s) 24, 56
AluBI AGCT 3 cut(s) 56, 66, 174
AluI AGCT 3 cut(s) 56, 66, 174
Alw21I GWGCWC 2 cut(s) 58, 509
AlwI GGATC 2 cut(s) 39, 52
ApoI RAATTY 1 cut(s) 195
AspLEI GCGC 1 cut(s) 126
BamHI GGATCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 58
Bbv12I GWGCWC 2 cut(s) 58, 509
BccI CCATC 1 cut(s) 92
BfaI CTAG 1 cut(s) 374
BmiI GGNNCC 2 cut(s) 46, 82
BmsI GCATC 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse1I ACTGG 2 cut(s) 48, 272
Bse3DI GCAATG 1 cut(s) 445
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 2 cut(s) 191, 522
BseNI ACTGG 2 cut(s) 48, 272
BseRI GAGGAG 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 58, 509
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 219
Bsp1286I GDGCHC 2 cut(s) 58, 509
Bsp143I GATC 2 cut(s) 44, 128
BspCNI CTCAG 2 cut(s) 192, 521
BspLI GGNNCC 2 cut(s) 46, 82
BspPI GGATC 2 cut(s) 39, 52
BsrDI GCAATG 1 cut(s) 445
BsrI ACTGG 2 cut(s) 48, 272
BssMI GATC 2 cut(s) 44, 128
Bst4CI ACNGT 4 cut(s) 184, 210, 431, 461
Bst6I CTCTTC 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 3 cut(s) 200, 464, 508
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 2 cut(s) 47, 131
BstMBI GATC 2 cut(s) 44, 128
BstMWI GCNNNNNNNGC 2 cut(s) 132, 242
BstNSI RCATGY 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 1 cut(s) 137
CfoI GCGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 457
CviAII CATG 2 cut(s) 19, 136
CviJI RGCY 6 cut(s) 56, 66, 174, 216, 317, 408
CviKI_1 RGCY 6 cut(s) 56, 66, 174, 216, 317, 408
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 3 cut(s) 200, 464, 508
DpnI GATC 2 cut(s) 46, 130
DpnII GATC 2 cut(s) 44, 128
DraI TTTAAA 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Ecl136II GAGCTC 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 58
FaeI CATG 2 cut(s) 22, 139
FaiI YATR 4 cut(s) 20, 137, 383, 470
FaqI GGGAC 1 cut(s) 219
FatI CATG 2 cut(s) 18, 135
FriOI GRGCYC 1 cut(s) 58
FspBI CTAG 1 cut(s) 374
GlaI GCGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 126
Hin1II CATG 2 cut(s) 22, 139
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 172
HinfI GANTC 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 168, 457
Hpy188I TCNGA 2 cut(s) 201, 511
Hpy188III TCNNGA 2 cut(s) 109, 178
Hpy8I GTNNAC 2 cut(s) 168, 457
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 2 cut(s) 26, 158
HpyCH4III ACNGT 4 cut(s) 184, 210, 431, 461
HpyCH4V TGCA 3 cut(s) 139, 245, 438
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 242
HpyF3I CTNAG 3 cut(s) 200, 464, 508
Hsp92II CATG 2 cut(s) 22, 139
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 44, 128
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 4 cut(s) 61, 117, 122, 285
LweI GCATC 1 cut(s) 357
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 3 cut(s) 143, 298, 425
MalI GATC 2 cut(s) 46, 130
MboI GATC 2 cut(s) 44, 128
MboII GAAGA 4 cut(s) 41, 244, 472, 475
MfeI CAATTG 1 cut(s) 439
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 58, 509
MluCI AATT 7 cut(s) 14, 96, 195, 360, 390, 399, 439
MmeI TCCRAC 1 cut(s) 22
MnlI CCTC 4 cut(s) 29, 195, 260, 398
MseI TTAA 5 cut(s) 60, 95, 104, 192, 411
MunI CAATTG 1 cut(s) 439
MwoI GCNNNNNNNGC 2 cut(s) 132, 242
NdeII GATC 2 cut(s) 44, 128
NlaIII CATG 2 cut(s) 22, 139
NlaIV GGNNCC 2 cut(s) 46, 82
NspI RCATGY 1 cut(s) 139
PaeI GCATGC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 46, 82
PsuI RGATCY 1 cut(s) 44
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SacI GAGCTC 1 cut(s) 58
SaqAI TTAA 5 cut(s) 60, 95, 104, 192, 411
Sau3AI GATC 2 cut(s) 44, 128
SduI GDGCHC 2 cut(s) 58, 509
SetI ASST 6 cut(s) 58, 68, 150, 176, 252, 307
SfaNI GCATC 1 cut(s) 357
SphI GCATGC 1 cut(s) 139
Sse9I AATT 7 cut(s) 14, 96, 195, 360, 390, 399, 439
SspMI CTAG 1 cut(s) 374
SstI GAGCTC 1 cut(s) 58
TaaI ACNGT 4 cut(s) 184, 210, 431, 461
TaqI TCGA 1 cut(s) 115
TasI AATT 7 cut(s) 14, 96, 195, 360, 390, 399, 439
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 5 cut(s) 60, 95, 104, 192, 411
Tru9I TTAA 5 cut(s) 60, 95, 104, 192, 411
TscAI CASTG 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 7, 216
TspRI CASTG 1 cut(s) 55
XapI RAATTY 1 cut(s) 195
XceI RCATGY 1 cut(s) 139
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.