MD15G1270400.v1.1

Cold acclimation protein WCOR413

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013591
Physical Location & Seq
Forward (+)
23487034 .. 23495644
8611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1270400.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGACATGGGGTTTGCTGGATTGGCGGCTGCTGCTGCAAATGTTGGATTTGGAGAAGCAGAAGATGGATTTGGATCGAGCGCGAGCAGGGCTCGAGCTGCCTTTCAATGGGGTGGAACCGTCTTTGCCTTAATTTTGTTAATCTTGAACCGAGCAGGGCGGAGATCAGCCATGCAAAGTAACTTTCTTGTACTGTACTTGTTTACAAGCTTCCCAACAGTGTTGTTCAAAATTTTGAGGGGGCAATTCGGTTATTGGGTTTCCTTTCTGGCTGTTGCCGCAAACCTCTTCTTTCCTCAAACTGTTCCAGTTTCTCGTTTTCTCTTGTTTGTCGTCACACCTGTCTGGGTCGCTAACGGACTGCGTGATAGTATCGTGGGCTGCATCTTTTGTCTAATACTCGGAGTTTCGCTGGTCATAACAGAGATACGAGGAATCGGAGGTTTTTGTAACTGTCAATGTAATTTTCATTGCTTTGCTTATTGTCTTTGTATAGGATTTATCTTCTTCTTTACAATATTGTATCTATGCTCAGAAACTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WCOR413

Protein Analysis

182

Amino Acids

19.85

Weight (kDa)

8.43

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WCOR413 PF05562 32 - 160 9.7e-28 Cold acclimation protein WCOR413
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013591)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14181 FvH4_1g14252
malus_domestica MD15G1270400.v1.1
prunus_persica Prupe.7G147700_v2.0.a1
pyrus_communis pycom15g23460
rosa_chinensis RchiOBHm_Chr2g0102851
rosa_laevigata RLG00000017184
rosa_multiflora Rmu_sc0000388.1_g000049
rosa_roxburghii Rroxscaffold_2G00140250
rosa_rugosa Rorug02G0110100
rosa_samantha Rh2AG158500 Rh2AG434100 Rh2BG164900 Rh2CG164600 Rh2CG419800 Rh2DG163700
rosa_wichuraiana Rw2G012330 Rw2G012380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 83
AciI CCGC 3 cut(s) 26, 160, 279
AclWI GGATC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 231
AfaI GTAC 2 cut(s) 192, 197
AfiI CCNNNNNNNGG 1 cut(s) 108
AgsI TTSAA 3 cut(s) 107, 148, 229
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 2 cut(s) 98, 210
AluI AGCT 2 cut(s) 98, 210
AlwI GGATC 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 93
ApeKI GCWGC 5 cut(s) 29, 32, 35, 98, 381
ApoI RAATTY 1 cut(s) 231
ArsI GACNNNNNNTTYG 1 cut(s) 28
AspLEI GCGC 1 cut(s) 83
AvaI CYCGRG 1 cut(s) 93
BanII GRGCYC 1 cut(s) 94
BbvI GCAGC 5 cut(s) 16, 19, 22, 85, 368
BccI CCATC 1 cut(s) 59
BisI GCNGC 7 cut(s) 27, 30, 33, 36, 99, 279, 382
BlsI GCNGC 7 cut(s) 28, 31, 34, 37, 100, 280, 383
BmeT110I CYCGRG 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 118
BmsI GCATC 1 cut(s) 393
BplI GAGNNNNNCTC 2 cut(s) 76, 108
BsaBI GATNNNNATC 1 cut(s) 73
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 469
Bse8I GATNNNNATC 1 cut(s) 73
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 1 cut(s) 308
BseXI GCAGC 5 cut(s) 16, 19, 22, 85, 368
Bsh1236I CGCG 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 94
Bsp143I GATC 2 cut(s) 74, 164
BspACI CCGC 3 cut(s) 26, 160, 279
BspCNI CTCAG 1 cut(s) 545
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 469
BsrI ACTGG 1 cut(s) 308
BssMI GATC 2 cut(s) 74, 164
Bst4CI ACNGT 5 cut(s) 121, 195, 220, 304, 455
Bst6I CTCTTC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 83
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 2 cut(s) 77, 167
BstMBI GATC 2 cut(s) 74, 164
BstMWI GCNNNNNNNGC 6 cut(s) 23, 32, 35, 89, 98, 278
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 16, 19, 22, 85, 368
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 191, 196
CviAII CATG 2 cut(s) 7, 172
CviJI RGCY 7 cut(s) 29, 92, 98, 170, 210, 272, 381
CviKI_1 RGCY 7 cut(s) 29, 92, 98, 170, 210, 272, 381
CviQI GTAC 2 cut(s) 191, 196
DdeI CTNAG 1 cut(s) 532
DpnI GATC 2 cut(s) 76, 166
DpnII GATC 2 cut(s) 74, 164
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 94
Eco88I CYCGRG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 94
FaeI CATG 2 cut(s) 10, 175
FaiI YATR 5 cut(s) 8, 173, 419, 494, 529
FatI CATG 2 cut(s) 6, 171
Fnu4HI GCNGC 7 cut(s) 27, 30, 33, 36, 99, 279, 382
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 7 cut(s) 27, 30, 33, 36, 99, 279, 382
GlaI GCGC 1 cut(s) 82
GluI GCNGC 7 cut(s) 27, 30, 33, 36, 99, 279, 382
HhaI GCGC 1 cut(s) 83
Hin1II CATG 2 cut(s) 10, 175
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 1 cut(s) 435
Hpy166II GTNNAC 1 cut(s) 204
Hpy188I TCNGA 3 cut(s) 404, 440, 535
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 1 cut(s) 204
HpyCH4III ACNGT 5 cut(s) 121, 195, 220, 304, 455
HpyCH4V TGCA 3 cut(s) 38, 175, 384
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 32, 35, 89, 98, 278
HpyF3I CTNAG 1 cut(s) 532
Hsp92II CATG 2 cut(s) 10, 175
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 74, 164
LpnPI CCDG 8 cut(s) 3, 73, 141, 254, 321, 331, 354, 398
Lsp1109I GCAGC 5 cut(s) 16, 19, 22, 85, 368
LweI GCATC 1 cut(s) 393
MaeIII GTNAC 3 cut(s) 179, 334, 449
MalI GATC 2 cut(s) 76, 166
MboI GATC 2 cut(s) 74, 164
MboII GAAGA 4 cut(s) 74, 280, 496, 499
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 4 cut(s) 132, 231, 245, 463
MmeI TCCRAC 1 cut(s) 25
MnlI CCTC 5 cut(s) 231, 296, 306, 425, 434
MseI TTAA 2 cut(s) 131, 140
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 6 cut(s) 23, 32, 35, 89, 98, 278
NdeII GATC 2 cut(s) 74, 164
NlaIII CATG 2 cut(s) 10, 175
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 334
PaeR7I CTCGAG 1 cut(s) 93
PfeI GAWTC 1 cut(s) 435
PkrI GCNGC 7 cut(s) 28, 31, 34, 37, 100, 280, 383
PspN4I GGNNCC 1 cut(s) 118
PspXI VCTCGAGB 1 cut(s) 93
RsaI GTAC 2 cut(s) 192, 197
RsaNI GTAC 2 cut(s) 191, 196
SaqAI TTAA 2 cut(s) 131, 140
SatI GCNGC 7 cut(s) 27, 30, 33, 36, 99, 279, 382
Sau3AI GATC 2 cut(s) 74, 164
SduI GDGCHC 1 cut(s) 94
SetI ASST 5 cut(s) 100, 212, 288, 343, 445
SfaNI GCATC 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 93
SlaI CTCGAG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
Sse9I AATT 4 cut(s) 132, 231, 245, 463
SsiI CCGC 3 cut(s) 26, 160, 279
SspI AATATT 1 cut(s) 519
TaaI ACNGT 5 cut(s) 121, 195, 220, 304, 455
TaqI TCGA 2 cut(s) 77, 94
TasI AATT 4 cut(s) 132, 231, 245, 463
TatI WGTACW 2 cut(s) 190, 195
TauI GCSGC 2 cut(s) 29, 281
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 2 cut(s) 131, 140
Tru9I TTAA 2 cut(s) 131, 140
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 5 cut(s) 29, 32, 35, 98, 381
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 458
TspGWI ACGGA 1 cut(s) 372
TspRI CASTG 1 cut(s) 225
XapI RAATTY 1 cut(s) 231
XhoI CTCGAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.