FvH4_1g15250

poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016239
Physical Location & Seq
Reverse (-)
8660360 .. 8664438
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15250.t1

Sequence Viewer

Length: 1362 bp
ATGGAAGCGAGGTTTGTGAGACCCAGGAGAACTAGTTTCATGGTTGATTGTGAAGATTCAATCATCAAAGTGGAAAATGGTAACCGGTACAAAAATGTGAAGAAGAAAATTGAAAAAGGCGGTATTGCTTTCCCAGAACAGATTCATATTGACACCGAGGATTATCACCAGGAGGAGGAGGTTTCTATCGTTGTGGATCAAAATTCCGGGTCGGATCGCACCGAATCTAAGCAGAACGATGAAGATTCCGAGTATTGCGCTGATTCGAATACCACGGATGATCAGTTTGGGGTTTTCACCAGAAATGGGATGATATTGAGGGTGAATGGATCGAGTGACGAATACGAGACTATTCTCAAGAGCTTTGGTGTTGGGATGGGATCGGCGGCAGGAGTTACGGAGGTTGTGGCGGTGCACAAGAATTTGGTTTCTGGATTGATCCGCCGGGCGAGGTTTGAAACATTTCGGGTGTTTTCACAGGCAGTGGGGAAGAAATGCGGTGGAAACCCGAACATTAGGTTTGCTTGGTACAGCGGATCGAGAGATGAGTATGCGTTGGATGGAGCAAGTGCATCACAGGTTGGTGATGATGGGCTCAGGCATATGCTGCTGTGCCGTGTTATACTGGGGAAGATGGAAGAGGTGCCTAGTGGTTCTGGACAAGTTGTTCCTGGTTCGAATGAGTTTGATTCTGGTGCGGATAATGTTGTGAATCCTCGAAGGTATTTTGTGTGGAGTCCATGCATGAACTCGCACATTTGTCCTGCTTATGTTGTGAGCTTCAAGGGTCCTGCTAGCTTGGAGGGTTATGTGACAAGTACTACTACTACTACTCAGACGGGAAGTATCGCTAGTGCTGTAACTCAGGGGAATGCTCGCTACAAGAGATCAGCGCTTGGAAATGTTACTCTTGTAGCTCTGCTGGCCGCGCTGACGAGATTCTTGACTCCTCGAGAAATGAAGATAGTTGTCACATTGCTGCAGGATTTCAGGGCAAAGAAGTTTGACGCAGACGAGTTGAAGCGAAAAGCGAGACTCATAGCTGGTGACAAGTTGATCTCGGCAATGAAATTGGCGGCTGTTAAGGGGAGTAGTACTCTTGGTGGCTTGCAGTTGGAGAATGGATCAGTGCGAGATATATATCTCCCTGGTCTGATAACCGTGCTTGCAGAGTTCTTGCCTGCTCAGAAGATTGAAATGCTTAGCAAAAGCCTTGGCGATTTCAAGGCTAAGAAGATAAATCGACCTCAGCTGGCTACAAGTGTGAGAGACATTGTAGGCAATGACAATGTGTTGGTTGCAGCAATCAACTCTTACAGAATCAGTAAGCACCGGCCGCAAGACAAGCAGTCAATTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

454

Amino Acids

49.67

Weight (kDa)

8.83

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 302 - 354 6.4e-08 RCD1-SRO-TAF4 (RST) plant domain
RST PF12174 382 - 440 1.4e-10 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016239)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15180 FvH4_1g15250 FvH4_6g03610 FvH4_6g03610
rosa_chinensis RchiOBHm_Chr3g0452151
rosa_laevigata RLG00000025624
rosa_multiflora Rmu_sc0039908.1_g000001
rosa_roxburghii Rroxscaffold_164G00436040 Rroxscaffold_6G00429270
rosa_rugosa Rorug02G0638600
rosa_samantha Rh3AG040600 Rh3BG042100 Rh3CG040500 Rh3DG041400
rosa_wichuraiana Rw3G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 643
AccII CGCG 1 cut(s) 929
AclWI GGATC 7 cut(s) 204, 222, 337, 388, 433, 544, 1132
AcoI YGGCCR 2 cut(s) 924, 1334
AcsI RAATTY 2 cut(s) 202, 421
AfaI GTAC 4 cut(s) 89, 530, 820, 1096
AfeI AGCGCT 1 cut(s) 894
AfiI CCNNNNNNNGG 2 cut(s) 175, 306
AgeI ACCGGT 1 cut(s) 84
AgsI TTSAA 7 cut(s) 60, 113, 458, 784, 1021, 1196, 1225
AhdI GACNNNNNGTC 1 cut(s) 1348
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 4 cut(s) 23, 168, 670, 1147
AloI GAACNNNNNNTCC 2 cut(s) 651, 683
AluBI AGCT 6 cut(s) 363, 780, 798, 917, 1043, 1252
AluI AGCT 6 cut(s) 363, 780, 798, 917, 1043, 1252
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 4 cut(s) 13, 341, 1027, 1263
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 7 cut(s) 204, 222, 337, 388, 433, 544, 1132
Ama87I CYCGRG 1 cut(s) 951
Aor51HI AGCGCT 1 cut(s) 894
AoxI GGCC 2 cut(s) 924, 1334
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 3 cut(s) 607, 979, 1301
ApoI RAATTY 2 cut(s) 202, 421
AsiGI ACCGGT 1 cut(s) 84
Asp700I GAANNNNTTC 2 cut(s) 141, 462
AspLEI GCGC 3 cut(s) 260, 895, 931
AspS9I GGNCC 1 cut(s) 788
AsuC2I CCSGG 2 cut(s) 208, 446
AsuHPI GGTGA 5 cut(s) 158, 289, 334, 596, 1058
AsuII TTCGAA 2 cut(s) 266, 677
AsuNHI GCTAGC 1 cut(s) 794
AvaI CYCGRG 1 cut(s) 951
AvaII GGWCC 1 cut(s) 788
BaeGI GKGCMC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 643
BanII GRGCYC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 417
BbvCI CCTCAGC 1 cut(s) 1248
BbvI GCAGC 3 cut(s) 594, 966, 1313
BccI CCATC 4 cut(s) 370, 554, 584, 628
BceAI ACGGC 1 cut(s) 600
BciT130I CCWGG 4 cut(s) 25, 170, 672, 1149
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 2 cut(s) 208, 446
BcoDI GTCTC 4 cut(s) 13, 341, 1027, 1263
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 4 cut(s) 33, 648, 795, 852
BfmI CTRYAG 1 cut(s) 980
BfoI RGCGCY 1 cut(s) 896
BisI GCNGC 7 cut(s) 387, 608, 927, 980, 1077, 1302, 1337
BlpI GCTNAGC 1 cut(s) 1202
BlsI GCNGC 7 cut(s) 388, 609, 928, 981, 1078, 1303, 1338
BmcAI AGTACT 2 cut(s) 820, 1096
Bme1390I CCNGG 6 cut(s) 25, 170, 208, 446, 672, 1149
Bme18I GGWCC 1 cut(s) 788
BmeRI GACNNNNNGTC 1 cut(s) 1348
BmeT110I CYCGRG 1 cut(s) 951
BmgT120I GGNCC 1 cut(s) 788
BmiI GGNNCC 2 cut(s) 645, 789
BmrFI CCNGG 6 cut(s) 25, 170, 208, 446, 672, 1149
BmrI ACTGGG 1 cut(s) 635
BmsI GCATC 1 cut(s) 581
BmtI GCTAGC 1 cut(s) 798
BmuI ACTGGG 1 cut(s) 635
BplI GAGNNNNNCTC 2 cut(s) 1081, 1113
Bpu10I CCTNAGC 2 cut(s) 596, 1248
Bpu1102I GCTNAGC 1 cut(s) 1202
Bpu14I TTCGAA 2 cut(s) 266, 677
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 2 cut(s) 208, 446
BsaBI GATNNNNATC 1 cut(s) 1140
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 5 cut(s) 23, 156, 273, 1147, 1213
BsaWI WCCGGW 1 cut(s) 84
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 306
Bse118I RCCGGY 2 cut(s) 84, 1332
Bse1I ACTGG 1 cut(s) 630
Bse3DI GCAATG 3 cut(s) 974, 1071, 1288
Bse8I GATNNNNATC 1 cut(s) 1140
BseBI CCWGG 4 cut(s) 25, 170, 672, 1149
BseDI CCNNGG 5 cut(s) 23, 156, 273, 1147, 1213
BseGI GGATG 4 cut(s) 283, 315, 381, 565
BseJI GATNNNNATC 1 cut(s) 1140
BseLI CCNNNNNNNGG 2 cut(s) 175, 306
BseMI GCAATG 3 cut(s) 974, 1071, 1288
BseMII CTCAG 5 cut(s) 610, 848, 878, 1199, 1262
BseNI ACTGG 1 cut(s) 630
BseRI GAGGAG 3 cut(s) 188, 191, 939
BseSI GKGCMC 1 cut(s) 417
BseX3I CGGCCG 1 cut(s) 1334
BseXI GCAGC 3 cut(s) 594, 966, 1313
Bsh1236I CGCG 1 cut(s) 929
Bsh1285I CGRYCG 1 cut(s) 1337
BshFI GGCC 2 cut(s) 926, 1336
BshNI GGYRCC 1 cut(s) 643
BshTI ACCGGT 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 1337
BsiHKAI GWGCWC 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 951
BsiSI CCGG 4 cut(s) 85, 207, 445, 1333
BslI CCNNNNNNNGG 2 cut(s) 175, 306
BsmAI GTCTC 4 cut(s) 13, 341, 1027, 1263
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 2 cut(s) 926, 1336
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 951
Bsp119I TTCGAA 2 cut(s) 266, 677
Bsp1286I GDGCHC 2 cut(s) 417, 597
Bsp1720I GCTNAGC 1 cut(s) 1202
BspANI GGCC 2 cut(s) 926, 1336
BspCNI CTCAG 5 cut(s) 609, 847, 877, 1198, 1261
BspFNI CGCG 1 cut(s) 929
BspLI GGNNCC 2 cut(s) 645, 789
BspMAI CTGCAG 1 cut(s) 984
BspOI GCTAGC 1 cut(s) 798
BspPI GGATC 7 cut(s) 204, 222, 337, 388, 433, 544, 1132
BspT104I TTCGAA 2 cut(s) 266, 677
BspT107I GGYRCC 1 cut(s) 643
BspTNI GGTCTC 1 cut(s) 13
BsrDI GCAATG 3 cut(s) 974, 1071, 1288
BsrFI RCCGGY 2 cut(s) 84, 1332
BsrI ACTGG 1 cut(s) 630
BssAI RCCGGY 2 cut(s) 84, 1332
BssECI CCNNGG 5 cut(s) 23, 156, 273, 1147, 1213
BssT1I CCWWGG 1 cut(s) 1213
Bst2UI CCWGG 4 cut(s) 25, 170, 672, 1149
Bst4CI ACNGT 1 cut(s) 1162
Bst6I CTCTTC 1 cut(s) 633
BstAPI GCANNNNNTGC 1 cut(s) 607
BstBI TTCGAA 2 cut(s) 266, 677
BstC8I GCNNGC 7 cut(s) 796, 877, 924, 1109, 1167, 1182, 1254
BstDEI CTNAG 8 cut(s) 228, 596, 834, 864, 1185, 1202, 1230, 1248
BstDSI CCRYGG 1 cut(s) 273
BstEII GGTNACC 1 cut(s) 80
BstF5I GGATG 4 cut(s) 283, 315, 381, 565
BstFNI CGCG 1 cut(s) 929
BstH2I RGCGCY 1 cut(s) 896
BstHHI GCGC 3 cut(s) 260, 895, 931
BstMAI GTCTC 4 cut(s) 13, 341, 1027, 1263
BstMCI CGRYCG 1 cut(s) 1337
BstMWI GCNNNNNNNGC 5 cut(s) 607, 923, 928, 1336, 1345
BstNI CCWGG 4 cut(s) 25, 170, 672, 1149
BstPI GGTNACC 1 cut(s) 80
BstSCI CCNGG 6 cut(s) 23, 168, 206, 444, 670, 1147
BstSFI CTRYAG 1 cut(s) 980
BstSLI GKGCMC 1 cut(s) 417
BstUI CGCG 1 cut(s) 929
BstV1I GCAGC 3 cut(s) 594, 966, 1313
BstZI CGGCCG 1 cut(s) 1334
BsuRI GGCC 2 cut(s) 926, 1336
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 4 cut(s) 283, 315, 381, 565
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 489, 1134
Cac8I GCNNGC 7 cut(s) 796, 877, 924, 1109, 1167, 1182, 1254
CfoI GCGC 3 cut(s) 260, 895, 931
Cfr10I RCCGGY 2 cut(s) 84, 1332
Cfr13I GGNCC 1 cut(s) 788
CseI GACGC 1 cut(s) 1016
Csp6I GTAC 4 cut(s) 88, 529, 819, 1095
CspAI ACCGGT 1 cut(s) 84
CviAII CATG 3 cut(s) 40, 741, 745
CviQI GTAC 4 cut(s) 88, 529, 819, 1095
DdeI CTNAG 8 cut(s) 228, 596, 834, 864, 1185, 1202, 1230, 1248
DriI GACNNNNNGTC 1 cut(s) 1348
EaeI YGGCCR 2 cut(s) 924, 1334
EagI CGGCCG 1 cut(s) 1334
Eam1104I CTCTTC 1 cut(s) 633
Eam1105I GACNNNNNGTC 1 cut(s) 1348
EarI CTCTTC 1 cut(s) 633
EciI GGCGGA 1 cut(s) 431
EclXI CGGCCG 1 cut(s) 1334
Eco130I CCWWGG 1 cut(s) 1213
Eco24I GRGCYC 1 cut(s) 597
Eco31I GGTCTC 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 788
Eco47III AGCGCT 1 cut(s) 894
Eco52I CGGCCG 1 cut(s) 1334
Eco88I CYCGRG 1 cut(s) 951
Eco91I GGTNACC 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 788
EcoO65I GGTNACC 1 cut(s) 80
EcoRII CCWGG 4 cut(s) 23, 168, 670, 1147
EcoT14I CCWWGG 1 cut(s) 1213
EcoT22I ATGCAT 1 cut(s) 746
EcoT38I GRGCYC 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 1213
FaeI CATG 3 cut(s) 43, 744, 748
FatI CATG 3 cut(s) 39, 740, 744
FauNDI CATATG 1 cut(s) 603
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 7 cut(s) 387, 608, 927, 980, 1077, 1302, 1337
FokI GGATG 4 cut(s) 290, 322, 388, 572
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 7 cut(s) 387, 608, 927, 980, 1077, 1302, 1337
FspBI CTAG 4 cut(s) 33, 648, 795, 852
GlaI GCGC 3 cut(s) 259, 894, 930
GluI GCNGC 7 cut(s) 387, 608, 927, 980, 1077, 1302, 1337
HaeII RGCGCY 1 cut(s) 896
HaeIII GGCC 2 cut(s) 926, 1336
HapII CCGG 4 cut(s) 85, 207, 445, 1333
HgaI GACGC 1 cut(s) 1016
HhaI GCGC 3 cut(s) 260, 895, 931
Hin1II CATG 3 cut(s) 43, 744, 748
Hin6I GCGC 3 cut(s) 258, 893, 929
HinP1I GCGC 3 cut(s) 258, 893, 929
HpaII CCGG 4 cut(s) 85, 207, 445, 1333
HphI GGTGA 5 cut(s) 158, 289, 334, 596, 1058
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 5 cut(s) 214, 250, 837, 1155, 1188
Hpy188III TCNNGA 6 cut(s) 358, 432, 540, 657, 943, 953
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 1 cut(s) 714
HpyCH4III ACNGT 1 cut(s) 1162
HpyCH4V TGCA 7 cut(s) 415, 572, 744, 982, 1111, 1169, 1301
HpyF10VI GCNNNNNNNGC 5 cut(s) 607, 923, 928, 1336, 1345
HpyF3I CTNAG 8 cut(s) 228, 596, 834, 864, 1185, 1202, 1230, 1248
Hsp92II CATG 3 cut(s) 43, 744, 748
HspAI GCGC 3 cut(s) 258, 893, 929
Ksp22I TGATCA 1 cut(s) 280
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 3 cut(s) 594, 966, 1313
LweI GCATC 1 cut(s) 581
MaeI CTAG 4 cut(s) 33, 648, 795, 852
MaeIII GTNAC 8 cut(s) 80, 335, 394, 811, 859, 904, 970, 1046
MfeI CAATTG 1 cut(s) 1353
MhlI GDGCHC 2 cut(s) 417, 597
MluCI AATT 5 cut(s) 108, 202, 421, 1070, 1353
MlyI GAGTC 3 cut(s) 745, 940, 1029
MmeI TCCRAC 3 cut(s) 192, 537, 1095
Mph1103I ATGCAT 1 cut(s) 746
MroXI GAANNNNTTC 2 cut(s) 141, 462
MseI TTAA 1 cut(s) 1083
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 2 cut(s) 534, 1252
MspI CCGG 4 cut(s) 85, 207, 445, 1333
MspR9I CCNGG 6 cut(s) 25, 170, 208, 446, 672, 1149
MunI CAATTG 1 cut(s) 1353
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 4 cut(s) 25, 170, 672, 1149
MvnI CGCG 1 cut(s) 929
MwoI GCNNNNNNNGC 5 cut(s) 607, 923, 928, 1336, 1345
NciI CCSGG 2 cut(s) 208, 446
NdeI CATATG 1 cut(s) 603
NheI GCTAGC 1 cut(s) 794
NlaIII CATG 3 cut(s) 43, 744, 748
NlaIV GGNNCC 2 cut(s) 645, 789
NmeAIII GCCGAG 1 cut(s) 1040
NmuCI GTSAC 4 cut(s) 335, 811, 970, 1046
NsiI ATGCAT 1 cut(s) 746
NspV TTCGAA 2 cut(s) 266, 677
PaeR7I CTCGAG 1 cut(s) 951
PctI GAATGC 1 cut(s) 877
PdmI GAANNNNTTC 2 cut(s) 141, 462
PfeI GAWTC 9 cut(s) 56, 142, 224, 245, 263, 689, 712, 939, 1320
PinAI ACCGGT 1 cut(s) 84
PkrI GCNGC 7 cut(s) 388, 609, 928, 981, 1078, 1303, 1338
PleI GAGTC 3 cut(s) 744, 940, 1029
PpsI GAGTC 3 cut(s) 744, 940, 1029
PpuMI RGGWCCY 1 cut(s) 788
Psp5II RGGWCCY 1 cut(s) 788
Psp6I CCWGG 4 cut(s) 23, 168, 670, 1147
PspEI GGTNACC 1 cut(s) 80
PspGI CCWGG 4 cut(s) 23, 168, 670, 1147
PspN4I GGNNCC 2 cut(s) 645, 789
PspPI GGNCC 1 cut(s) 788
PspPPI RGGWCCY 1 cut(s) 788
PstI CTGCAG 1 cut(s) 984
PvuII CAGCTG 1 cut(s) 1252
RsaI GTAC 4 cut(s) 89, 530, 820, 1096
RsaNI GTAC 4 cut(s) 88, 529, 819, 1095
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 1 cut(s) 1083
SatI GCNGC 7 cut(s) 387, 608, 927, 980, 1077, 1302, 1337
Sau96I GGNCC 1 cut(s) 788
ScaI AGTACT 2 cut(s) 820, 1096
SchI GAGTC 3 cut(s) 745, 940, 1029
ScrFI CCNGG 6 cut(s) 25, 170, 208, 446, 672, 1149
SduI GDGCHC 2 cut(s) 417, 597
SfaNI GCATC 1 cut(s) 581
SfcI CTRYAG 1 cut(s) 980
Sfr274I CTCGAG 1 cut(s) 951
SfuI TTCGAA 2 cut(s) 266, 677
SinI GGWCC 1 cut(s) 788
SlaI CTCGAG 1 cut(s) 951
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 2 cut(s) 356, 951
SmoI CTYRAG 2 cut(s) 356, 951
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 5 cut(s) 108, 202, 421, 1070, 1353
SspMI CTAG 4 cut(s) 33, 648, 795, 852
StyD4I CCNGG 6 cut(s) 23, 168, 206, 444, 670, 1147
StyI CCWWGG 1 cut(s) 1213
TaaI ACNGT 1 cut(s) 1162
TaqI TCGA 7 cut(s) 266, 332, 539, 677, 718, 952, 1243
TasI AATT 5 cut(s) 108, 202, 421, 1070, 1353
TatI WGTACW 2 cut(s) 818, 1094
TauI GCSGC 4 cut(s) 389, 929, 1079, 1339
TfiI GAWTC 9 cut(s) 56, 142, 224, 245, 263, 689, 712, 939, 1320
Tru1I TTAA 1 cut(s) 1083
Tru9I TTAA 1 cut(s) 1083
TscAI CASTG 2 cut(s) 489, 1134
TseFI GTSAC 4 cut(s) 335, 811, 970, 1046
TseI GCWGC 3 cut(s) 607, 979, 1301
Tsp45I GTSAC 4 cut(s) 335, 811, 970, 1046
TspDTI ATGAA 6 cut(s) 28, 134, 255, 761, 974, 1082
TspGWI ACGGA 2 cut(s) 290, 413
TspRI CASTG 2 cut(s) 489, 1134
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 788
XapI RAATTY 2 cut(s) 202, 421
XhoI CTCGAG 1 cut(s) 951
XmnI GAANNNNTTC 2 cut(s) 141, 462
XspI CTAG 4 cut(s) 33, 648, 795, 852
ZrmI AGTACT 2 cut(s) 820, 1096
Zsp2I ATGCAT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.