FvH4_6g03610

poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016239
Physical Location & Seq
Reverse (-)
1981474 .. 1983582
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03610.t2

Sequence Viewer

Length: 1347 bp
ATGGAGCAGATCCAGATTGCCACCGATGATATCCACAAGGAGGAGGAGGAGGTTTCTCCGATGGTAGAGGATCACGATTCCGGGTCGGATCGCTCCGAATCGGACCATAACGATCAAAATTCCGACGAGTCTGATTGCGAGAGCGAGGCGGCGGATTCGAATCCCGGTGATGATAATGCCGACGATCAATTTAGGGTTTTCACCAGAAATGGGTTTATGTCTCGAGTGGATGGATCGAGCGCGCAATACGAGACGATTCTCAAGAGCTTTGGTGTGGGGATGGGATCGGCTGCGGGAGTTACGGAGGTTGTGGCGGTGCACAAGTTTCTGGTGTCCGCAATGGCCGGTGGGCCCAGGGTCCGGATATTTCGGGGGTTTGCGAACGCGGTGGGGACCAAATGCGGGGGGAACCCCAACATTAGGTATGCTTGGTATGGCGGATCGAGGGATGAGATTGCTCGGATTGTGGCTCATGGGTTTGGTAGTTTGAATGGTGACGAGTGTGGTCGGATTCAACTGAATGCGGTGAAGTTTGCGTTGGATGGGGCAAAGGCGTTGGAGGTTGGTGATGATGGGCTCAGGCATATGCTGCTGTGCCTTGTTGTATTGGGGAAGATGGAGGAGTTGCCTAGTTGTTCTGCACAGGTTAAAGCTAGTTCGGATGAGTTTGATACTGGTGTGGATTGTGTTGTGAATCCTCGGAGGTATTTTGTGTGGAGTAATTGCATGAACTACGCTTGTCCTGCTTTTGTGGTGAGCTTCAAGGGTCCTGCTAGCTTGGAGGGTTATGCGACGACGGCAAGTACTACTACTACTCAGAGGGGGAGAGTCGCTAGCGCTAGCGCTATGGGGAATGCTCTCAACAAGCGATCAGCGCGTGGAGGATATATTTCTCTTGGTGCTCTGGTGGCCGCGCTGACGAGGTTCTTGTCTCCGAGAGAAATGAAGATAATGGTCAGATTGCTGGAAGATTTCAGGGCAAAGAAGTTTGACGTAGAAGAGTTGAAGCGAAAAGCGAAGCTCATAGCTGGTGACAAGTTGTTCTCGGCAATGAAATTGGCGGCTGTTAAGGGGAGTGGTACTGTTGGTGGCTTGCAGCTGGTGAATGGATCAGTACGAGATATATATCTCCCTGGTCTGATAACAGTGCTTGCAGAGTTCTTGCCTGCTACGAAGATTGAAATGCTTAGCAAAAGCCTTGGGGATTTCAAGGATAAGAAGATAAATCGATCTCAGCTGGCTAATAGTGTGAGAGCCATTGTTGGCAATGACAATATGTTGGTTGCAGCAATCAACTCTTACAGAATCAAAAAGCAGCGGTTGCAAGACAAGCAGTCAGTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

449

Amino Acids

48.4

Weight (kDa)

6.63

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 297 - 348 1.5e-06 RCD1-SRO-TAF4 (RST) plant domain
RST PF12174 377 - 435 2.9e-11 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016239)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15180 FvH4_1g15250 FvH4_6g03610 FvH4_6g03610
rosa_chinensis RchiOBHm_Chr3g0452151
rosa_laevigata RLG00000025624
rosa_multiflora Rmu_sc0039908.1_g000001
rosa_roxburghii Rroxscaffold_164G00436040 Rroxscaffold_6G00429270
rosa_rugosa Rorug02G0638600
rosa_samantha Rh3AG040600 Rh3BG042100 Rh3CG040500 Rh3DG041400
rosa_wichuraiana Rw3G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 242, 386, 877, 914
AccIII TCCGGA 1 cut(s) 360
AclWI GGATC 7 cut(s) 4, 78, 96, 241, 292, 448, 1117
AcoI YGGCCR 2 cut(s) 342, 909
AcsI RAATTY 1 cut(s) 118
AfaI GTAC 3 cut(s) 805, 1081, 1116
AfeI AGCGCT 2 cut(s) 838, 844
AfiI CCNNNNNNNGG 5 cut(s) 40, 210, 360, 402, 420
AgsI TTSAA 6 cut(s) 490, 515, 763, 1006, 1181, 1210
AhdI GACNNNNNGTC 1 cut(s) 1333
AjnI CCWGG 2 cut(s) 353, 1132
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
AluBI AGCT 8 cut(s) 267, 653, 759, 777, 1021, 1028, 1099, 1237
AluI AGCT 8 cut(s) 267, 653, 759, 777, 1021, 1028, 1099, 1237
Alw21I GWGCWC 2 cut(s) 321, 904
Alw26I GTCTC 3 cut(s) 225, 245, 936
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 7 cut(s) 4, 78, 96, 241, 292, 448, 1117
Ama87I CYCGRG 1 cut(s) 222
Aor13HI TCCGGA 1 cut(s) 360
Aor51HI AGCGCT 2 cut(s) 838, 844
AoxI GGCC 3 cut(s) 342, 350, 909
ApaI GGGCCC 1 cut(s) 354
ApaLI GTGCAC 1 cut(s) 317
ApeKI GCWGC 5 cut(s) 290, 589, 1096, 1286, 1315
ApoI RAATTY 1 cut(s) 118
AspLEI GCGC 6 cut(s) 242, 244, 839, 845, 877, 916
AspS9I GGNCC 6 cut(s) 103, 350, 351, 358, 393, 767
AsuC2I CCSGG 2 cut(s) 82, 165
AsuHPI GGTGA 8 cut(s) 179, 193, 506, 538, 578, 766, 1043, 1114
AsuII TTCGAA 1 cut(s) 158
AsuNHI GCTAGC 3 cut(s) 773, 833, 839
AvaI CYCGRG 1 cut(s) 222
AvaII GGWCC 4 cut(s) 103, 358, 393, 767
BaeGI GKGCMC 2 cut(s) 321, 354
BaeI ACNNNNGTAYC 2 cut(s) 663, 696
BanII GRGCYC 2 cut(s) 354, 579
Bbv12I GWGCWC 2 cut(s) 321, 904
BbvI GCAGC 5 cut(s) 277, 576, 1108, 1298, 1327
BccI CCATC 6 cut(s) 55, 224, 274, 536, 566, 610
BceAI ACGGC 1 cut(s) 813
BciT130I CCWGG 2 cut(s) 355, 1134
BcnI CCSGG 2 cut(s) 82, 165
BcoDI GTCTC 3 cut(s) 225, 245, 936
BfaI CTAG 5 cut(s) 630, 654, 774, 834, 840
BfoI RGCGCY 2 cut(s) 840, 846
BisI GCNGC 8 cut(s) 150, 291, 590, 912, 1062, 1097, 1287, 1316
BlpI GCTNAGC 1 cut(s) 1187
BlsI GCNGC 8 cut(s) 151, 292, 591, 913, 1063, 1098, 1288, 1317
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 4 cut(s) 82, 165, 355, 1134
Bme18I GGWCC 4 cut(s) 103, 358, 393, 767
BmeRI GACNNNNNGTC 1 cut(s) 1333
BmeT110I CYCGRG 1 cut(s) 222
BmgT120I GGNCC 6 cut(s) 103, 350, 351, 358, 393, 767
BmiI GGNNCC 5 cut(s) 352, 359, 394, 410, 768
BmrFI CCNGG 4 cut(s) 82, 165, 355, 1134
BmtI GCTAGC 3 cut(s) 777, 837, 843
Bpu10I CCTNAGC 1 cut(s) 578
Bpu1102I GCTNAGC 1 cut(s) 1187
Bpu14I TTCGAA 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 82, 165
Bsa29I ATCGAT 1 cut(s) 1228
BsaBI GATNNNNATC 2 cut(s) 159, 1125
BsaJI CCNNGG 5 cut(s) 353, 354, 698, 1132, 1198
BsaWI WCCGGW 1 cut(s) 360
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 210, 360, 402, 420
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 3 cut(s) 345, 1056, 1273
Bse8I GATNNNNATC 2 cut(s) 159, 1125
BseAI TCCGGA 1 cut(s) 360
BseBI CCWGG 2 cut(s) 355, 1134
BseCI ATCGAT 1 cut(s) 1228
BseDI CCNNGG 5 cut(s) 353, 354, 698, 1132, 1198
BseGI GGATG 5 cut(s) 235, 285, 454, 547, 667
BseJI GATNNNNATC 2 cut(s) 159, 1125
BseLI CCNNNNNNNGG 5 cut(s) 40, 210, 360, 402, 420
BseMI GCAATG 3 cut(s) 345, 1056, 1273
BseMII CTCAG 3 cut(s) 592, 830, 1247
BseNI ACTGG 1 cut(s) 679
BsePI GCGCGC 1 cut(s) 240
BseRI GAGGAG 4 cut(s) 56, 59, 62, 635
BseSI GKGCMC 2 cut(s) 321, 354
BseXI GCAGC 5 cut(s) 277, 576, 1108, 1298, 1327
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 4 cut(s) 242, 386, 877, 914
BshFI GGCC 3 cut(s) 344, 352, 911
BshVI ATCGAT 1 cut(s) 1228
BsiHKAI GWGCWC 2 cut(s) 321, 904
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 4 cut(s) 81, 165, 345, 361
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 5 cut(s) 40, 210, 360, 402, 420
BsmAI GTCTC 3 cut(s) 225, 245, 936
BsmBI CGTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 406
BsmI GAATGC 2 cut(s) 526, 859
BsnI GGCC 3 cut(s) 344, 352, 911
BsoBI CYCGRG 1 cut(s) 222
Bsp119I TTCGAA 1 cut(s) 158
Bsp120I GGGCCC 1 cut(s) 350
Bsp1286I GDGCHC 4 cut(s) 321, 354, 579, 904
Bsp13I TCCGGA 1 cut(s) 360
Bsp1720I GCTNAGC 1 cut(s) 1187
BspANI GGCC 3 cut(s) 344, 352, 911
BspCNI CTCAG 3 cut(s) 591, 829, 1246
BspDI ATCGAT 1 cut(s) 1228
BspEI TCCGGA 1 cut(s) 360
BspFNI CGCG 4 cut(s) 242, 386, 877, 914
BspLI GGNNCC 5 cut(s) 352, 359, 394, 410, 768
BspOI GCTAGC 3 cut(s) 777, 837, 843
BspPI GGATC 7 cut(s) 4, 78, 96, 241, 292, 448, 1117
BspT104I TTCGAA 1 cut(s) 158
BsrDI GCAATG 3 cut(s) 345, 1056, 1273
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 1 cut(s) 679
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 5 cut(s) 353, 354, 698, 1132, 1198
BssHII GCGCGC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 1198
Bst2UI CCWGG 2 cut(s) 355, 1134
Bst4CI ACNGT 2 cut(s) 1084, 1147
Bst6I CTCTTC 1 cut(s) 993
BstAPI GCANNNNNTGC 2 cut(s) 589, 1321
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 8 cut(s) 242, 775, 835, 841, 1094, 1152, 1167, 1239
BstDEI CTNAG 4 cut(s) 578, 816, 1187, 1233
BstF5I GGATG 5 cut(s) 235, 285, 454, 547, 667
BstFNI CGCG 4 cut(s) 242, 386, 877, 914
BstH2I RGCGCY 2 cut(s) 840, 846
BstHHI GCGC 6 cut(s) 242, 244, 839, 845, 877, 916
BstMAI GTCTC 3 cut(s) 225, 245, 936
BstMWI GCNNNNNNNGC 7 cut(s) 589, 743, 797, 874, 908, 1321, 1330
BstNI CCWGG 2 cut(s) 355, 1134
BstSCI CCNGG 4 cut(s) 80, 163, 353, 1132
BstSLI GKGCMC 2 cut(s) 321, 354
BstUI CGCG 4 cut(s) 242, 386, 877, 914
BstV1I GCAGC 5 cut(s) 277, 576, 1108, 1298, 1327
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
Bsu15I ATCGAT 1 cut(s) 1228
BsuRI GGCC 3 cut(s) 344, 352, 911
BsuTUI ATCGAT 1 cut(s) 1228
BtsCI GGATG 5 cut(s) 235, 285, 454, 547, 667
BtsIMutI CAGTG 1 cut(s) 1152
Cac8I GCNNGC 8 cut(s) 242, 775, 835, 841, 1094, 1152, 1167, 1239
CfoI GCGC 6 cut(s) 242, 244, 839, 845, 877, 916
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 6 cut(s) 103, 350, 351, 358, 393, 767
ClaI ATCGAT 1 cut(s) 1228
Csp6I GTAC 3 cut(s) 804, 1080, 1115
CviAII CATG 2 cut(s) 473, 727
CviQI GTAC 3 cut(s) 804, 1080, 1115
DdeI CTNAG 4 cut(s) 578, 816, 1187, 1233
DriI GACNNNNNGTC 1 cut(s) 1333
EaeI YGGCCR 2 cut(s) 342, 909
Eam1104I CTCTTC 1 cut(s) 993
Eam1105I GACNNNNNGTC 1 cut(s) 1333
EarI CTCTTC 1 cut(s) 993
EciI GGCGGA 2 cut(s) 167, 453
Eco130I CCWWGG 1 cut(s) 1198
Eco24I GRGCYC 2 cut(s) 354, 579
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 4 cut(s) 103, 358, 393, 767
Eco47III AGCGCT 2 cut(s) 838, 844
Eco88I CYCGRG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 767
EcoRII CCWGG 2 cut(s) 353, 1132
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 1198
EcoT38I GRGCYC 2 cut(s) 354, 579
ErhI CCWWGG 1 cut(s) 1198
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 2 cut(s) 476, 730
FaqI GGGAC 1 cut(s) 406
FatI CATG 2 cut(s) 472, 726
FauI CCCGC 2 cut(s) 286, 395
FauNDI CATATG 1 cut(s) 585
Fnu4HI GCNGC 8 cut(s) 150, 291, 590, 912, 1062, 1097, 1287, 1316
FokI GGATG 5 cut(s) 242, 292, 461, 554, 674
FriOI GRGCYC 2 cut(s) 354, 579
Fsp4HI GCNGC 8 cut(s) 150, 291, 590, 912, 1062, 1097, 1287, 1316
FspBI CTAG 5 cut(s) 630, 654, 774, 834, 840
GlaI GCGC 6 cut(s) 241, 243, 838, 844, 876, 915
GluI GCNGC 8 cut(s) 150, 291, 590, 912, 1062, 1097, 1287, 1316
HaeII RGCGCY 2 cut(s) 840, 846
HaeIII GGCC 3 cut(s) 344, 352, 911
HapII CCGG 4 cut(s) 81, 165, 345, 361
HhaI GCGC 6 cut(s) 242, 244, 839, 845, 877, 916
Hin1II CATG 2 cut(s) 476, 730
Hin6I GCGC 6 cut(s) 240, 242, 837, 843, 875, 914
HinP1I GCGC 6 cut(s) 240, 242, 837, 843, 875, 914
HpaII CCGG 4 cut(s) 81, 165, 345, 361
HphI GGTGA 8 cut(s) 179, 193, 506, 538, 578, 766, 1043, 1114
Hpy166II GTNNAC 1 cut(s) 319
Hpy188III TCNNGA 5 cut(s) 13, 74, 222, 262, 361
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 4 cut(s) 128, 185, 796, 799
HpyCH4III ACNGT 2 cut(s) 1084, 1147
HpyCH4IV ACGT 1 cut(s) 993
HpyCH4V TGCA 7 cut(s) 319, 641, 726, 1096, 1154, 1286, 1324
HpyF10VI GCNNNNNNNGC 7 cut(s) 589, 743, 797, 874, 908, 1321, 1330
HpyF3I CTNAG 4 cut(s) 578, 816, 1187, 1233
HpySE526I ACGT 1 cut(s) 993
Hsp92II CATG 2 cut(s) 476, 730
HspAI GCGC 6 cut(s) 240, 242, 837, 843, 875, 914
Kpn2I TCCGGA 1 cut(s) 360
LmnI GCTCC 2 cut(s) 4, 98
Lsp1109I GCAGC 5 cut(s) 277, 576, 1108, 1298, 1327
MaeI CTAG 5 cut(s) 630, 654, 774, 834, 840
MaeII ACGT 1 cut(s) 993
MaeIII GTNAC 3 cut(s) 298, 494, 1031
MboII GAAGA 6 cut(s) 625, 958, 980, 1010, 1186, 1231
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 4 cut(s) 321, 354, 579, 904
MluCI AATT 4 cut(s) 118, 188, 721, 1055
MlyI GAGTC 2 cut(s) 137, 837
MmeI TCCRAC 5 cut(s) 66, 147, 488, 519, 537
MroI TCCGGA 1 cut(s) 360
MseI TTAA 2 cut(s) 648, 1068
MspA1I CMGCKG 3 cut(s) 1099, 1237, 1318
MspI CCGG 4 cut(s) 81, 165, 345, 361
MspR9I CCNGG 4 cut(s) 82, 165, 355, 1134
Mva1269I GAATGC 2 cut(s) 526, 859
MvaI CCWGG 2 cut(s) 355, 1134
MvnI CGCG 4 cut(s) 242, 386, 877, 914
MwoI GCNNNNNNNGC 7 cut(s) 589, 743, 797, 874, 908, 1321, 1330
NciI CCSGG 2 cut(s) 82, 165
NdeI CATATG 1 cut(s) 585
NheI GCTAGC 3 cut(s) 773, 833, 839
NlaIII CATG 2 cut(s) 476, 730
NlaIV GGNNCC 5 cut(s) 352, 359, 394, 410, 768
NmeAIII GCCGAG 1 cut(s) 1025
NmuCI GTSAC 2 cut(s) 494, 1031
NspV TTCGAA 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 222
PasI CCCWGGG 1 cut(s) 354
PauI GCGCGC 1 cut(s) 240
PctI GAATGC 2 cut(s) 526, 859
PfeI GAWTC 8 cut(s) 77, 98, 155, 160, 256, 511, 694, 1305
PkrI GCNGC 8 cut(s) 151, 292, 591, 913, 1063, 1098, 1288, 1317
PleI GAGTC 2 cut(s) 136, 836
PpsI GAGTC 2 cut(s) 136, 836
PpuMI RGGWCCY 1 cut(s) 767
Psp5II RGGWCCY 1 cut(s) 767
Psp6I CCWGG 2 cut(s) 353, 1132
PspGI CCWGG 2 cut(s) 353, 1132
PspN4I GGNNCC 5 cut(s) 352, 359, 394, 410, 768
PspOMI GGGCCC 1 cut(s) 350
PspPI GGNCC 6 cut(s) 103, 350, 351, 358, 393, 767
PspPPI RGGWCCY 1 cut(s) 767
PsuI RGATCY 1 cut(s) 9
PteI GCGCGC 1 cut(s) 240
PvuII CAGCTG 2 cut(s) 1099, 1237
RsaI GTAC 3 cut(s) 805, 1081, 1116
RsaNI GTAC 3 cut(s) 804, 1080, 1115
SaqAI TTAA 2 cut(s) 648, 1068
SatI GCNGC 8 cut(s) 150, 291, 590, 912, 1062, 1097, 1287, 1316
Sau96I GGNCC 6 cut(s) 103, 350, 351, 358, 393, 767
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 2 cut(s) 137, 837
ScrFI CCNGG 4 cut(s) 82, 165, 355, 1134
SduI GDGCHC 4 cut(s) 321, 354, 579, 904
Sfr274I CTCGAG 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 158
SinI GGWCC 4 cut(s) 103, 358, 393, 767
SlaI CTCGAG 1 cut(s) 222
SmlI CTYRAG 2 cut(s) 222, 260
SmoI CTYRAG 2 cut(s) 222, 260
Sse9I AATT 4 cut(s) 118, 188, 721, 1055
SspMI CTAG 5 cut(s) 630, 654, 774, 834, 840
StyD4I CCNGG 4 cut(s) 80, 163, 353, 1132
StyI CCWWGG 1 cut(s) 1198
TaaI ACNGT 2 cut(s) 1084, 1147
TaiI ACGT 1 cut(s) 996
TaqI TCGA 5 cut(s) 158, 223, 236, 443, 1228
TasI AATT 4 cut(s) 118, 188, 721, 1055
TatI WGTACW 1 cut(s) 803
TauI GCSGC 3 cut(s) 152, 914, 1064
TfiI GAWTC 8 cut(s) 77, 98, 155, 160, 256, 511, 694, 1305
Tru1I TTAA 2 cut(s) 648, 1068
Tru9I TTAA 2 cut(s) 648, 1068
TscAI CASTG 1 cut(s) 1152
TseFI GTSAC 2 cut(s) 494, 1031
TseI GCWGC 5 cut(s) 290, 589, 1096, 1286, 1315
Tsp45I GTSAC 2 cut(s) 494, 1031
TspDTI ATGAA 3 cut(s) 743, 959, 1067
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 1152
VneI GTGCAC 1 cut(s) 317
VpaK11BI GGWCC 4 cut(s) 103, 358, 393, 767
XapI RAATTY 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 222
XspI CTAG 5 cut(s) 630, 654, 774, 834, 840
ZrmI AGTACT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.