FvH4_1g16750

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012508
Physical Location & Seq
Reverse (-)
9689706 .. 9694249
4544 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16750.t2

Sequence Viewer

Length: 2364 bp
ATGACCACCCTAAACCCCATTCTTCTTCTTCTTCCGCTTCTTCTAGCTCAATGCTCTTATGGGCTCAACACAGATGGACTTCTTCTCCTCTCTTTCAAACTCTCCATCCTCCAAGACCCTCAGCTAGTCCTCCAAAATTGGAACCCCAACGACGAAACACCATGCTCATGGCGAGGTGTCACCTGCGCCACAATTCTCACTCCTCCTGAGAATGTCTTCTACCCTAGAGTCATCTCCCTAGCTCTCCCAAGCTCTGAGCTTGTGGGTTCGATCCCTGCCAATCTTGGCATGATCGAACAGCTCCAAAGCCTCAATCTTTCCGATAATTCCCTCAATGGGTCTCTCCCATTGTCTCTCTTCAACTCGACCCAGCTTCAAGTTCTTGACTTGGCTTTTAATCGAATCGCTGGCGTGTTGCCTGAAGCCGTTTCAGAGCTACCCAGAAGTCTCAGTCTTCTTAATCTTTCTGACAATGCCATGGAGGGTAAGATACCCAGTGATCTTGGCTCTCTGACCAACTTGACGGTGATTTCTTTGAAGAATAATTACTTCTCCGGCGAAATTCCGGCTGGGTTTGCAGCCGTGGAGGTTCTGGATCTGTCGTCCAATCTGATCAACGGATCTCTGCCGTCCGATTTCGGCGGCGACAGTCTCCGTTACTTTAACGTCTCCCACAACGAACTTTCCGGCGAAATCCCGGCGAGTTTCGCGGAGAAATTCCCGGGTAATGCGACTCTTGATCTCTCCTTCAACAACCTCACCGGCGCAATCCCGGAATCTCGGGTTTTCTTGAACCAGGAAACGGAGTCGTTTCGCGGCAACCCTGATCTCTGCGGCGAGGAAACAGAGCACAACCCTTGCCCTATTCCTTCTTCGCCGTCTTCCAACTCACCCAACTCTCCTCCGGCGTTTGCCGCGATTCCCAGAACGATGAACTCCTCCGATGACGATGCAGTTTCGCCGGGATCGGCAAAAGGGTCGTCGGGAAAATCCCAAACCGGGCTCAAGACGGCGACCATCGTCGGAATTGTGGCCGGCGATGTTGCCGGGATTGCTGTTATTGCGATGGTGTTCTTGTACGTATACAGAATGAGGAGGAAGAAGAAGAAGGAGAAGACAGCAAGCGGCGACGTCACGACGCTGAAGAACGAACCCAACAACCCAGTAGTGACGGATTGGTCTTCTTCTTCATCAGAATCAAGAGGGTTTACACGGTGGTCTTGCCTGAGAAACAAGAAGGGCGAAACGGAGGAGCAGAGCTCCGATTCCAGCAGCTCTGATAGTGAAGAAGCTCAAACGGAGCAGAATGGTCACAAGGGTAGTGAGAGCAAGCGGCAAACAGAGTCAGAACAGAGCAAAGGAGGCACATTGGTGACACTCGACGGCGAAAAGCAGCTTGAGCTCGAGACTCTGCTCAAAGCATCAGCCTACATTTTGGGTGCTACTGGTTCTAGCATAATGTACAAGGCGGTTTTGGAAGATGGGAGTAGTCTAGCGGTTCGGAGAATCGGAGAGCATAGTGTGGATCGGTTCAAGGATTTCGAGAACCAAATCCGGCTGGTAGCGAAATTGGTGCACCCAAATTTGGTTCGTGTTCGTGGATTCTTTTGGGGAGTTGATGAGAAGCTCATCATCTATGATTTCGTCCCAAATGGCAGCCTCGCCAATGCTCGTTACAGGAAGGTGGGCTCGTCGCCTTGTCATCTACCATGGGAAGCGAGGCTGAGGATAGCAAAGGGGGTGGCCCGTGGGCTCGGCTACCTCCACGAGAAGAAGCACGTCCATGGGAATCTCAAGCCCACCAACATTCTCCTCGGTGCCGATATGGAGCCCAAAATCGGCGATTTTGGTCTCGAGAGACTGTTGTCCGGCGACACGAGTTATAAGATTGGAAGCTCGACGAGGAACTTCGGAAGCAAGAGGTCCACGGCTTCGCGTGACAGCTTTCAAGATTTCACACTCGGGCCTAGCCCGGGCCCGAGTCCGAGCCCTAGCGCGATGGGGACGTCGCCCTATCATGCGCCCGAGTCGCTTCGGAGCCTCAAGCCCCACCCCAAGTGGGATGTGTTCTCGTTTGGGATCATATTGCTTGAGTTGATAACGGGGAAAGTTGTGGTTGTGGATGAGTTGGGCCAAGGTCTTGGGCTTTCGATCGATGATCCAAACCGCGCTTTTCGGATGGCTGATATGGCTATTCGAGGCGAGTTGGAGGGCAAGGAGGAACCCTTGTTGGCTCTCTTCAAGTTAGGGTACAGTTGTGCATCACCAGTTCCACAAAAGAGACCAGCAATGAAAGAAGCCCTACAAGCTCTTGAGAAATTTCCATGTTCACCTTCATCATCATATTATTATGGCCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

788

Amino Acids

85.09

Weight (kDa)

5.72

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 22 - 62 5.2e-13 Leucine rich repeat N-terminal domain
LRR_8 PF13855 124 - 183 5e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1884, 2362
AarI CACCTGC 1 cut(s) 191
AasI GACNNNNNNGTC 1 cut(s) 1177
AatII GACGTC 2 cut(s) 1134, 2009
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 1817
AccI GTMKAC 1 cut(s) 1083
AccII CGCG 6 cut(s) 710, 816, 917, 1936, 1997, 2169
AclWI GGATC 7 cut(s) 265, 603, 628, 973, 1533, 2087, 2153
AcoI YGGCCR 2 cut(s) 1032, 2353
AcsI RAATTY 4 cut(s) 561, 716, 1582, 2318
AcuI CTGAAG 2 cut(s) 441, 1163
AcyI GRCGYC 2 cut(s) 1131, 2006
AfaI GTAC 3 cut(s) 1079, 1463, 2252
AfiI CCNNNNNNNGG 7 cut(s) 336, 802, 999, 1585, 1838, 1973, 2059
AgsI TTSAA 9 cut(s) 97, 361, 377, 538, 751, 793, 1534, 1949, 2242
AjiI CACGTC 1 cut(s) 1780
AjnI CCWGG 1 cut(s) 795
AleI CACNNNNGTG 1 cut(s) 1370
Alw21I GWGCWC 4 cut(s) 852, 1262, 1404, 1578
Alw26I GTCTC 9 cut(s) 345, 357, 452, 656, 673, 1400, 1852, 1856, 2275
Alw44I GTGCAC 1 cut(s) 1574
AlwI GGATC 7 cut(s) 265, 603, 628, 973, 1533, 2087, 2153
Ama87I CYCGRG 8 cut(s) 721, 780, 1403, 1853, 1961, 1972, 1978, 2024
AoxI GGCC 6 cut(s) 1032, 1743, 1964, 1975, 2131, 2353
ApaI GGGCCC 1 cut(s) 1979
ApaLI GTGCAC 1 cut(s) 1574
ApeKI GCWGC 4 cut(s) 578, 1272, 1393, 1656
ApoI RAATTY 4 cut(s) 561, 716, 1582, 2318
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 5 cut(s) 188, 767, 1997, 2023, 2171
AspS9I GGNCC 6 cut(s) 1744, 1923, 1964, 1975, 1976, 2131
AsuC2I CCSGG 9 cut(s) 698, 722, 723, 773, 963, 1000, 1048, 1973, 1974
AsuHPI GGTGA 7 cut(s) 172, 538, 751, 882, 1384, 2256, 2322
AvaI CYCGRG 8 cut(s) 721, 780, 1403, 1853, 1961, 1972, 1978, 2024
AvaII GGWCC 1 cut(s) 1923
BaeGI GKGCMC 2 cut(s) 1578, 1979
BaeI ACNNNNGTAYC 2 cut(s) 2242, 2275
BalI TGGCCA 1 cut(s) 2355
BanI GGYRCC 1 cut(s) 1817
BanII GRGCYC 9 cut(s) 66, 1005, 1262, 1404, 1691, 1755, 1833, 1979, 1991
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 2 cut(s) 1766, 1876
BbsI GAAGAC 5 cut(s) 208, 446, 873, 1121, 1173
Bbv12I GWGCWC 4 cut(s) 852, 1262, 1404, 1578
BbvCI CCTCAGC 2 cut(s) 120, 1724
BbvI GCAGC 4 cut(s) 590, 1284, 1405, 1668
BccI CCATC 7 cut(s) 68, 113, 1025, 1060, 1475, 1993, 2173
BceAI ACGGC 7 cut(s) 410, 566, 613, 862, 1026, 1399, 1944
BcgI CGANNNNNNTGC 6 cut(s) 932, 960, 966, 994, 1555, 1589
BciT130I CCWGG 1 cut(s) 797
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 9 cut(s) 698, 722, 723, 773, 963, 1000, 1048, 1973, 1974
BcoDI GTCTC 9 cut(s) 345, 357, 452, 656, 673, 1400, 1852, 1856, 2275
BfaI CTAG 8 cut(s) 44, 125, 225, 239, 1452, 1493, 1968, 1992
BfuAI ACCTGC 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 1923
BmeT110I CYCGRG 8 cut(s) 721, 780, 1403, 1853, 1961, 1972, 1978, 2024
BmgBI CACGTC 1 cut(s) 1780
BmgT120I GGNCC 6 cut(s) 1744, 1923, 1964, 1975, 1976, 2131
BmiI GGNNCC 6 cut(s) 143, 1819, 1830, 1977, 2039, 2223
BmrI ACTGGG 2 cut(s) 489, 1157
BmsI GCATC 3 cut(s) 940, 1430, 2270
BmuI ACTGGG 2 cut(s) 489, 1157
BoxI GACNNNNGTC 2 cut(s) 1019, 1864
BpiI GAAGAC 5 cut(s) 208, 446, 873, 1121, 1173
BplI GAGNNNNNCTC 2 cut(s) 1244, 1276
Bpu10I CCTNAGC 2 cut(s) 120, 1724
BpuEI CTTGAG 6 cut(s) 989, 1418, 1778, 2027, 2111, 2333
BpuMI CCSGG 9 cut(s) 698, 722, 723, 773, 963, 1000, 1048, 1973, 1974
Bsa29I ATCGAT 1 cut(s) 2154
BsaAI YACGTR 1 cut(s) 1081
BsaHI GRCGYC 2 cut(s) 1131, 2006
BsaI GGTCTC 3 cut(s) 345, 1856, 2275
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 7 cut(s) 336, 802, 999, 1585, 1838, 1973, 2059
Bse118I RCCGGY 2 cut(s) 761, 1034
Bse1I ACTGG 4 cut(s) 495, 1163, 1450, 2267
Bse3DI GCAATG 1 cut(s) 2295
BseBI CCWGG 1 cut(s) 797
BseCI ATCGAT 1 cut(s) 2154
BseGI GGATG 4 cut(s) 105, 2068, 2128, 2184
BseLI CCNNNNNNNGG 7 cut(s) 336, 802, 999, 1585, 1838, 1973, 2059
BseMI GCAATG 1 cut(s) 2295
BseMII CTCAG 6 cut(s) 134, 198, 246, 463, 1217, 1715
BseNI ACTGG 4 cut(s) 495, 1163, 1450, 2267
BseRI GAGGAG 7 cut(s) 77, 192, 891, 928, 1108, 1265, 1802
BseSI GKGCMC 2 cut(s) 1578, 1979
BseXI GCAGC 4 cut(s) 590, 1284, 1405, 1668
BseYI CCCAGC 2 cut(s) 369, 569
Bsh1236I CGCG 6 cut(s) 710, 816, 917, 1936, 1997, 2169
Bsh1285I CGRYCG 1 cut(s) 2154
BshFI GGCC 6 cut(s) 1034, 1745, 1966, 1977, 2133, 2355
BshNI GGYRCC 1 cut(s) 1817
BshVI ATCGAT 1 cut(s) 2154
BsiEI CGRYCG 1 cut(s) 2154
BsiHKAI GWGCWC 4 cut(s) 852, 1262, 1404, 1578
BsiHKCI CYCGRG 8 cut(s) 721, 780, 1403, 1853, 1961, 1972, 1978, 2024
BslFI GGGAC 2 cut(s) 1631, 2017
BslI CCNNNNNNNGG 7 cut(s) 336, 802, 999, 1585, 1838, 1973, 2059
BsmAI GTCTC 9 cut(s) 345, 357, 452, 656, 673, 1400, 1852, 1856, 2275
BsmBI CGTCTC 1 cut(s) 673
BsmFI GGGAC 2 cut(s) 1631, 2017
BsnI GGCC 6 cut(s) 1034, 1745, 1966, 1977, 2133, 2355
Bso31I GGTCTC 3 cut(s) 345, 1856, 2275
BsoBI CYCGRG 8 cut(s) 721, 780, 1403, 1853, 1961, 1972, 1978, 2024
Bsp120I GGGCCC 1 cut(s) 1975
Bsp1407I TGTACA 1 cut(s) 1461
Bsp19I CCATGG 3 cut(s) 477, 1709, 1783
BspANI GGCC 6 cut(s) 1034, 1745, 1966, 1977, 2133, 2355
BspCNI CTCAG 6 cut(s) 133, 199, 247, 462, 1218, 1716
BspDI ATCGAT 1 cut(s) 2154
BspFNI CGCG 6 cut(s) 710, 816, 917, 1936, 1997, 2169
BspLI GGNNCC 6 cut(s) 143, 1819, 1830, 1977, 2039, 2223
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 7 cut(s) 265, 603, 628, 973, 1533, 2087, 2153
BspT107I GGYRCC 1 cut(s) 1817
BspTNI GGTCTC 3 cut(s) 345, 1856, 2275
BsrDI GCAATG 1 cut(s) 2295
BsrFI RCCGGY 2 cut(s) 761, 1034
BsrGI TGTACA 1 cut(s) 1461
BsrI ACTGG 4 cut(s) 495, 1163, 1450, 2267
BssAI RCCGGY 2 cut(s) 761, 1034
BssNAI GTATAC 1 cut(s) 1084
BssNI GRCGYC 2 cut(s) 1131, 2006
BssSI CACGAG 2 cut(s) 1766, 1876
BssT1I CCWWGG 4 cut(s) 477, 1709, 1783, 2134
Bst1107I GTATAC 1 cut(s) 1084
Bst2BI CACGAG 2 cut(s) 1766, 1876
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 5 cut(s) 526, 650, 1215, 1863, 2255
Bst6I CTCTTC 2 cut(s) 362, 2243
BstACI GRCGYC 2 cut(s) 1131, 2006
BstAUI TGTACA 1 cut(s) 1461
BstBAI YACGTR 1 cut(s) 1081
BstC8I GCNNGC 4 cut(s) 409, 1036, 1123, 1331
BstDEI CTNAG 6 cut(s) 120, 207, 255, 449, 1226, 1724
BstDSI CCRYGG 6 cut(s) 477, 582, 1709, 1747, 1783, 1926
BstF5I GGATG 4 cut(s) 105, 2068, 2128, 2184
BstFNI CGCG 6 cut(s) 710, 816, 917, 1936, 1997, 2169
BstHHI GCGC 5 cut(s) 188, 767, 1997, 2023, 2171
BstMAI GTCTC 9 cut(s) 345, 357, 452, 656, 673, 1400, 1852, 1856, 2275
BstMCI CGRYCG 1 cut(s) 2154
BstNI CCWGG 1 cut(s) 797
BstPAI GACNNNNGTC 2 cut(s) 1019, 1864
BstSLI GKGCMC 2 cut(s) 1578, 1979
BstSNI TACGTA 1 cut(s) 1081
BstUI CGCG 6 cut(s) 710, 816, 917, 1936, 1997, 2169
BstV1I GCAGC 4 cut(s) 590, 1284, 1405, 1668
BstV2I GAAGAC 5 cut(s) 208, 446, 873, 1121, 1173
BstX2I RGATCY 2 cut(s) 595, 620
BstXI CCANNNNNNTGG 2 cut(s) 168, 2141
BstYI RGATCY 2 cut(s) 595, 620
BstZ17I GTATAC 1 cut(s) 1084
Bsu15I ATCGAT 1 cut(s) 2154
BsuRI GGCC 6 cut(s) 1034, 1745, 1966, 1977, 2133, 2355
BsuTUI ATCGAT 1 cut(s) 2154
BtgI CCRYGG 6 cut(s) 477, 582, 1709, 1747, 1783, 1926
BtgZI GCGATG 3 cut(s) 1053, 1079, 2012
BtrI CACGTC 1 cut(s) 1780
BtsCI GGATG 4 cut(s) 105, 2068, 2128, 2184
BtsIMutI CAGTG 1 cut(s) 502
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 4 cut(s) 409, 1036, 1123, 1331
CfoI GCGC 5 cut(s) 188, 767, 1997, 2023, 2171
Cfr10I RCCGGY 2 cut(s) 761, 1034
Cfr13I GGNCC 6 cut(s) 1744, 1923, 1964, 1975, 1976, 2131
Cfr9I CCCGGG 2 cut(s) 721, 1972
ClaI ATCGAT 1 cut(s) 2154
CseI GACGC 1 cut(s) 1147
Csp6I GTAC 3 cut(s) 1078, 1462, 2251
CspCI CAANNNNNGTGG 2 cut(s) 1722, 1757
CviAII CATG 8 cut(s) 162, 168, 289, 478, 1710, 1784, 2018, 2325
CviQI GTAC 3 cut(s) 1078, 1462, 2251
DdeI CTNAG 6 cut(s) 120, 207, 255, 449, 1226, 1724
DrdI GACNNNNNNGTC 1 cut(s) 1177
DseDI GACNNNNNNGTC 1 cut(s) 1177
EaeI YGGCCR 2 cut(s) 1032, 2353
Eam1104I CTCTTC 2 cut(s) 362, 2243
EarI CTCTTC 2 cut(s) 362, 2243
Ecl136II GAGCTC 2 cut(s) 1260, 1402
Eco105I TACGTA 1 cut(s) 1081
Eco130I CCWWGG 4 cut(s) 477, 1709, 1783, 2134
Eco24I GRGCYC 9 cut(s) 66, 1005, 1262, 1404, 1691, 1755, 1833, 1979, 1991
Eco31I GGTCTC 3 cut(s) 345, 1856, 2275
Eco47I GGWCC 1 cut(s) 1923
Eco53kI GAGCTC 2 cut(s) 1260, 1402
Eco57I CTGAAG 2 cut(s) 441, 1163
Eco88I CYCGRG 8 cut(s) 721, 780, 1403, 1853, 1961, 1972, 1978, 2024
EcoICRI GAGCTC 2 cut(s) 1260, 1402
EcoRII CCWGG 1 cut(s) 795
EcoT14I CCWWGG 4 cut(s) 477, 1709, 1783, 2134
EcoT38I GRGCYC 9 cut(s) 66, 1005, 1262, 1404, 1691, 1755, 1833, 1979, 1991
ErhI CCWWGG 4 cut(s) 477, 1709, 1783, 2134
Esp3I CGTCTC 1 cut(s) 673
FaeI CATG 8 cut(s) 165, 171, 292, 481, 1713, 1787, 2021, 2328
FaqI GGGAC 2 cut(s) 1631, 2017
FatI CATG 8 cut(s) 161, 167, 288, 477, 1709, 1783, 2017, 2324
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 1 cut(s) 1083
FokI GGATG 4 cut(s) 92, 2075, 2135, 2191
FriOI GRGCYC 9 cut(s) 66, 1005, 1262, 1404, 1691, 1755, 1833, 1979, 1991
FspBI CTAG 8 cut(s) 44, 125, 225, 239, 1452, 1493, 1968, 1992
GlaI GCGC 5 cut(s) 187, 766, 1996, 2022, 2170
GsaI CCCAGC 2 cut(s) 373, 573
HaeIII GGCC 6 cut(s) 1034, 1745, 1966, 1977, 2133, 2355
HgaI GACGC 1 cut(s) 1147
HhaI GCGC 5 cut(s) 188, 767, 1997, 2023, 2171
Hin1I GRCGYC 2 cut(s) 1131, 2006
Hin1II CATG 8 cut(s) 165, 171, 292, 481, 1713, 1787, 2021, 2328
Hin6I GCGC 5 cut(s) 186, 765, 1995, 2021, 2169
HinP1I GCGC 5 cut(s) 186, 765, 1995, 2021, 2169
HphI GGTGA 7 cut(s) 172, 538, 751, 882, 1384, 2256, 2322
Hpy166II GTNNAC 5 cut(s) 1084, 1209, 1576, 1926, 2330
Hpy8I GTNNAC 5 cut(s) 1084, 1209, 1576, 1926, 2330
Hpy99I CGWCG 9 cut(s) 155, 985, 1025, 1133, 1141, 1385, 1696, 1903, 2011
HpyAV CCTTC 6 cut(s) 757, 879, 1102, 1231, 1675, 2343
HpyCH4III ACNGT 5 cut(s) 526, 650, 1215, 1863, 2255
HpyCH4IV ACGT 5 cut(s) 666, 1080, 1131, 1779, 2006
HpyCH4V TGCA 4 cut(s) 578, 953, 1576, 2261
HpyF3I CTNAG 6 cut(s) 120, 207, 255, 449, 1226, 1724
HpySE526I ACGT 5 cut(s) 666, 1080, 1131, 1779, 2006
Hsp92I GRCGYC 2 cut(s) 1131, 2006
Hsp92II CATG 8 cut(s) 165, 171, 292, 481, 1713, 1787, 2021, 2328
HspAI GCGC 5 cut(s) 186, 765, 1995, 2021, 2169
KroI GCCGGC 1 cut(s) 1034
KroNI GCCGGC 1 cut(s) 1036
Ksp22I TGATCA 1 cut(s) 612
LmnI GCTCC 6 cut(s) 306, 1252, 1265, 1300, 1828, 2037
Lsp1109I GCAGC 4 cut(s) 590, 1284, 1405, 1668
LweI GCATC 3 cut(s) 940, 1430, 2270
MaeI CTAG 8 cut(s) 44, 125, 225, 239, 1452, 1493, 1968, 1992
MaeII ACGT 5 cut(s) 666, 1080, 1131, 1779, 2006
MaeIII GTNAC 8 cut(s) 178, 656, 1132, 1168, 1310, 1372, 1673, 1937
MflI RGATCY 2 cut(s) 595, 620
MlsI TGGCCA 1 cut(s) 2355
MluNI TGGCCA 1 cut(s) 2355
MlyI GAGTC 7 cut(s) 237, 727, 815, 1352, 1402, 1990, 2036
MmeI TCCRAC 3 cut(s) 909, 1003, 2187
Mox20I TGGCCA 1 cut(s) 2355
MroNI GCCGGC 1 cut(s) 1034
MroXI GAANNNNTTC 1 cut(s) 215
MscI TGGCCA 1 cut(s) 2355
MseI TTAA 3 cut(s) 396, 459, 663
MslI CAYNNNNRTG 3 cut(s) 166, 1370, 1782
Msp20I TGGCCA 1 cut(s) 2355
MvaI CCWGG 1 cut(s) 797
MvnI CGCG 6 cut(s) 710, 816, 917, 1936, 1997, 2169
NaeI GCCGGC 1 cut(s) 1036
NciI CCSGG 9 cut(s) 698, 722, 723, 773, 963, 1000, 1048, 1973, 1974
NcoI CCATGG 3 cut(s) 477, 1709, 1783
NgoMIV GCCGGC 1 cut(s) 1034
NlaIII CATG 8 cut(s) 165, 171, 292, 481, 1713, 1787, 2021, 2328
NlaIV GGNNCC 6 cut(s) 143, 1819, 1830, 1977, 2039, 2223
NmeAIII GCCGAG 1 cut(s) 1734
NmuCI GTSAC 6 cut(s) 178, 1132, 1168, 1310, 1372, 1937
OliI CACNNNNGTG 1 cut(s) 1370
PaeR7I CTCGAG 2 cut(s) 1403, 1853
PaqCI CACCTGC 1 cut(s) 191
PdiI GCCGGC 1 cut(s) 1036
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 8 cut(s) 402, 776, 919, 1196, 1265, 1506, 1602, 1789
PfoI TCCNGGA 1 cut(s) 771
Ple19I CGATCG 1 cut(s) 2154
PleI GAGTC 7 cut(s) 236, 727, 814, 1351, 1402, 1989, 2035
PpsI GAGTC 7 cut(s) 236, 727, 814, 1351, 1402, 1989, 2035
Ppu21I YACGTR 1 cut(s) 1081
PshAI GACNNNNGTC 2 cut(s) 1019, 1864
PsiI TTATAA 2 cut(s) 1884, 2362
Psp124BI GAGCTC 2 cut(s) 1262, 1404
Psp6I CCWGG 1 cut(s) 795
PspFI CCCAGC 2 cut(s) 369, 569
PspGI CCWGG 1 cut(s) 795
PspN4I GGNNCC 6 cut(s) 143, 1819, 1830, 1977, 2039, 2223
PspOMI GGGCCC 1 cut(s) 1975
PspPI GGNCC 6 cut(s) 1744, 1923, 1964, 1975, 1976, 2131
PsuI RGATCY 2 cut(s) 595, 620
PvuI CGATCG 1 cut(s) 2154
RsaI GTAC 3 cut(s) 1079, 1463, 2252
RsaNI GTAC 3 cut(s) 1078, 1462, 2251
RseI CAYNNNNRTG 3 cut(s) 166, 1370, 1782
SacI GAGCTC 2 cut(s) 1262, 1404
SaqAI TTAA 3 cut(s) 396, 459, 663
Sau96I GGNCC 6 cut(s) 1744, 1923, 1964, 1975, 1976, 2131
SchI GAGTC 7 cut(s) 237, 727, 815, 1352, 1402, 1990, 2036
SfaNI GCATC 3 cut(s) 940, 1430, 2270
Sfr274I CTCGAG 2 cut(s) 1403, 1853
SgrAI CRCCGGYG 1 cut(s) 761
SinI GGWCC 1 cut(s) 1923
SlaI CTCGAG 2 cut(s) 1403, 1853
SmaI CCCGGG 2 cut(s) 723, 1974
SmiMI CAYNNNNRTG 3 cut(s) 166, 1370, 1782
SmlI CTYRAG 8 cut(s) 1004, 1397, 1403, 1793, 1853, 2042, 2090, 2312
SmoI CTYRAG 8 cut(s) 1004, 1397, 1403, 1793, 1853, 2042, 2090, 2312
SnaBI TACGTA 1 cut(s) 1081
SrfI GCCCGGGC 1 cut(s) 1974
SspMI CTAG 8 cut(s) 44, 125, 225, 239, 1452, 1493, 1968, 1992
SstI GAGCTC 2 cut(s) 1262, 1404
StyI CCWWGG 4 cut(s) 477, 1709, 1783, 2134
TaaI ACNGT 5 cut(s) 526, 650, 1215, 1863, 2255
TaiI ACGT 5 cut(s) 669, 1083, 1134, 1782, 2009
TatI WGTACW 1 cut(s) 1461
TauI GCSGC 6 cut(s) 645, 819, 837, 917, 1128, 1336
TfiI GAWTC 8 cut(s) 402, 776, 919, 1196, 1265, 1506, 1602, 1789
Tru1I TTAA 3 cut(s) 396, 459, 663
Tru9I TTAA 3 cut(s) 396, 459, 663
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 6 cut(s) 178, 1132, 1168, 1310, 1372, 1937
TseI GCWGC 4 cut(s) 578, 1272, 1393, 1656
Tsp45I GTSAC 6 cut(s) 178, 1132, 1168, 1310, 1372, 1937
TspDTI ATGAA 4 cut(s) 947, 1179, 2306, 2325
TspGWI ACGGA 6 cut(s) 633, 644, 818, 1187, 1262, 1313
TspMI CCCGGG 2 cut(s) 721, 1972
TspRI CASTG 1 cut(s) 502
VneI GTGCAC 1 cut(s) 1574
VpaK11BI GGWCC 1 cut(s) 1923
XapI RAATTY 4 cut(s) 561, 716, 1582, 2318
XhoI CTCGAG 2 cut(s) 1403, 1853
XmaI CCCGGG 2 cut(s) 721, 1972
XmiI GTMKAC 1 cut(s) 1083
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 8 cut(s) 44, 125, 225, 239, 1452, 1493, 1968, 1992
ZraI GACGTC 2 cut(s) 1132, 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.