Rh2BG199500

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012508
Physical Location & Seq
Reverse (-)
18300282 .. 18303681
3400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG199500.1

Sequence Viewer

Length: 2367 bp
ATGACCTCCCTAAACCCCACTCCTCTTCTTCTTCTTCTTCTTCTTCTACTTCTCACTCTTCTAGCTCAATGCTCTTCTGGGCTCGACACAGACGGTCTTCTTCTCCTCTCCTTCAAACTCTCTATCCTCCAAGACCCTCAGCGAGTTCTTCAAACTTGGAACCCCTACGATACAACACCATGTTCATGGCGAGGTGTGACCTGCGCCACAATTCTCACTCCTCCTGAAAATATCTTCTACCCTAGAGTCATAGGCCTAGCTCTGCCCAGCTCTGCGCTTGTGGGTTCGATCCCTGCCAATCTTGGCATGATCGAACACCTTCAAAGCCTCAATCTTTCTGACAATTCCCTCAATGGGTCTCTTCCACAGTCTTTGTTCAACGCAACCGAGCTTCAAGTTTTGGACTTGGCATTTAATCGAATACTTGGTCCGCTACCAGAAACCATTTCAGGGCCCAAAAAACTCCAGCTTCTTAACCTCTCTGATAATGCCTTGGAGGGGAAGATACCCAGTAATCTTGCTTCTCTGAGCAACTTGACTGTGATTTCTCTCAAGAACAATTACTTCTCCGGCGAAATTCCGACTGGGTTTGCAGCCGTGGAGGTTTTGGATCTGTCGTCGAATCTGATCAACGGATCTCTGCCGTCGGATTTCGGCGGCGACAGTCTCCGTTACTTTAACGTCTCCCACAACGGAATCTCCGGCGCAATCCCAGCTAACTTCGCCGAGAAAATTCCCGGTAATGCGACCCTTGATCTTTCCTTCAACAACCTCACCGGCGAAGTCCCGGAGTCTCCGGCTTTCTTGAACCAGGAAACGGCGGCGTTTCGCGGCAACCCTGATCTGTGCGGCGAAGCAACCGAGAACAACCCGTGCCCTATTCCTTCTTCACCTTCTTCCAATGCACCCAATTCTCCTCCAGCTTTTGCCGCAATTCCCAGAACGAGAAATTCCACCAACGCAGATGAAACTTCGCCGGGATCGTCGGAAAAATCCCAATCCGGGCTCAAAGCGGCGACGATCGTCGGAATCGTGGCCGGCGACATTGCTGGGATTGCAATTATCGCGATGGTGTTCTTGTACATGTACAGATTGAGGAAGAAAAAGAAGGAGAAGGAGAAGACAACAAGCGGCGACGTCACTACGCTGAAGAAAGAAGCCAACCCAGTAGTGACGGATTGGTCTTCTTCTTCATCAGAATCAAGAGGGTTCACGCGGTGGTCTTGCTTGAGAAACAAGAAGGGCGAACCAGACGAGCAGAGCTCCGATTCCAGCAGCTCTTACAGCGAAGAAGTTCAAACGGAGCAGAATGGTCAGAAGGGTAGTGAGAGTAAACGGCAAACAGAGCAAGAACAGAGCAAAGGCGGCACATTGGTGACCCTGGACGGCGAAAAGCAGCTAGAGCTGGAGACTCTGCTTAAAGCATCAGCTTACATTTTGGGTGCTACTGGTTCTAGTATAATGTACAAGGCGGTTTTGGAAGACGGGAGTAGTCTAGCGGTTCGGAGAATCGGCGAGCATAGTGTCGACCGGTTCAAGGATTTCGAGAACCAAATCCGGCTAGTAGCGAAATTGGTCCACCCAAATCTGGTTCGAGTTCGTGGGTTCTTTTGGGGAGTTGATGAAAAGCTCATCATCTATGATTTCGTCCCAAATGGCAGCCTCGCCAATGCTCGTTACAGGAAGGTGGGCTCGTCGCCTTGTCATCTACCATGGGAAGCGAGGTTGAGGATAGCCAAGGGGGTGGCCCGTGGGCTGGGGTACCTCCACGAGAAGAAGCACGTCCACGGGAATCTCAAGCCCACCAACATTCTCCTCGGCGCTGATATGGAGCCCAAAGTCGGTGATTTTGGCCTCGAGAGGCTCTTGTCCGGTGATACGAGCTACAAAATAGGGAGCTCGACTAGGAATTTCGGAAGCAAGAGGTCGACGGCTTCACGTGATAGCTTTCAAGATTTCGCGTTAGGACCCAGTCCGGGCCCTAGTCCGAGCCCTAGCTCGATGGGGACGTCGCCCTATCATGCGCCCGAGTCACTTCGTAGCCTAAAGCCCCAACCCAAGTGGGATGTGTACTCATTTGGGGTCATATTGCTGGAGTTGATAACGGGAAAAGTTGTGGTTGTGGATGAGTTGGGCCAGGGTCTTGGGCTAGCCGTTGATGATCCAAACCGCGCTTTTCGGATGGCGGATATGGCGATTAGAGGCGAGTTGGAGGGCAAAGAGGAACCCTTACTGGCTCTCTTCAAGTTAGGGTACAGTTGTGCGTCACCAGTTCCACAAAAGAGACCAGCAATGAAAGAAGCCCTACAAGTTCTTGAGAAATTTCCATGTTCACCTTCATCATCATATTATTATGGCCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

788

Amino Acids

85.09

Weight (kDa)

6.28

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 5.3e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 130 - 188 2.5e-07 Leucine rich repeat
Pkinase PF00069 480 - 770 3.7e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 495 - 632 6.2e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2365
AasI GACNNNNNNGTC 1 cut(s) 1180
AatII GACGTC 2 cut(s) 1140, 2012
Acc36I ACCTGC 1 cut(s) 209
Acc65I GGTACC 1 cut(s) 1761
AccB1I GGYRCC 1 cut(s) 1761
AccI GTMKAC 2 cut(s) 1527, 1928
AccII CGCG 5 cut(s) 831, 1067, 1216, 1961, 2172
AclWI GGATC 5 cut(s) 283, 618, 643, 988, 2156
AcoI YGGCCR 2 cut(s) 1035, 2356
AcsI RAATTY 5 cut(s) 576, 732, 949, 1909, 2321
AcuI CTGAAG 1 cut(s) 1169
AcvI CACGTG 1 cut(s) 1940
AcyI GRCGYC 2 cut(s) 1137, 2009
AdeI CACNNNGTG 1 cut(s) 1218
AfaI GTAC 6 cut(s) 1082, 1088, 1466, 1763, 2072, 2255
AflIII ACRYGT 1 cut(s) 1083
AgeI ACCGGT 1 cut(s) 1530
AjiI CACGTC 1 cut(s) 1783
AjnI CCWGG 3 cut(s) 810, 1380, 2136
AleI CACNNNNGTG 1 cut(s) 1373
Alw21I GWGCWC 2 cut(s) 1265, 1901
Alw26I GTCTC 6 cut(s) 363, 671, 688, 798, 1403, 2278
AlwI GGATC 5 cut(s) 283, 618, 643, 988, 2156
Ama87I CYCGRG 2 cut(s) 1856, 2027
AoxI GGCC 8 cut(s) 253, 452, 1035, 1746, 1852, 1978, 2134, 2356
ApaI GGGCCC 2 cut(s) 456, 1982
ApeKI GCWGC 4 cut(s) 593, 1275, 1396, 1659
ApoI RAATTY 5 cut(s) 576, 732, 949, 1909, 2321
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
AsiGI ACCGGT 1 cut(s) 1530
Asp700I GAANNNNTTC 2 cut(s) 318, 1293
Asp718I GGTACC 1 cut(s) 1761
AspLEI GCGC 6 cut(s) 206, 277, 707, 1823, 2026, 2174
AspS9I GGNCC 9 cut(s) 428, 452, 453, 1576, 1747, 1967, 1978, 1979, 2134
AsuC2I CCSGG 5 cut(s) 738, 788, 978, 1003, 1977
AsuHPI GGTGA 7 cut(s) 766, 882, 1387, 1856, 1886, 2259, 2325
AsuNHI GCTAGC 1 cut(s) 2149
AvaI CYCGRG 2 cut(s) 1856, 2027
AvaII GGWCC 3 cut(s) 428, 1576, 1967
BaeGI GKGCMC 3 cut(s) 456, 878, 1982
BaeI ACNNNNGTAYC 2 cut(s) 2245, 2278
BalI TGGCCA 1 cut(s) 2358
BanI GGYRCC 1 cut(s) 1761
BanII GRGCYC 9 cut(s) 84, 456, 1008, 1265, 1694, 1836, 1901, 1982, 1994
BarI GAAGNNNNNNTAC 2 cut(s) 505, 537
BauI CACGAG 1 cut(s) 1769
BbrPI CACGTG 1 cut(s) 1940
BbsI GAAGAC 4 cut(s) 89, 1127, 1176, 1488
Bbv12I GWGCWC 2 cut(s) 1265, 1901
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 4 cut(s) 605, 1287, 1408, 1671
BccI CCATC 3 cut(s) 1063, 1996, 2176
BceAI ACGGC 7 cut(s) 581, 628, 834, 1352, 1402, 1947, 2138
BciT130I CCWGG 3 cut(s) 812, 1382, 2138
BclI TGATCA 1 cut(s) 627
BcnI CCSGG 5 cut(s) 738, 788, 978, 1003, 1977
BcoDI GTCTC 6 cut(s) 363, 671, 688, 798, 1403, 2278
BfoI RGCGCY 1 cut(s) 1824
BfuAI ACCTGC 1 cut(s) 209
Bme1390I CCNGG 8 cut(s) 738, 788, 812, 978, 1003, 1382, 1977, 2138
Bme18I GGWCC 3 cut(s) 428, 1576, 1967
BmeT110I CYCGRG 2 cut(s) 1856, 2027
BmgBI CACGTC 1 cut(s) 1783
BmgT120I GGNCC 9 cut(s) 428, 452, 453, 1576, 1747, 1967, 1978, 1979, 2134
BmiI GGNNCC 7 cut(s) 161, 454, 1763, 1833, 1969, 1980, 2226
BmrFI CCNGG 8 cut(s) 738, 788, 812, 978, 1003, 1382, 1977, 2138
BmrI ACTGGG 4 cut(s) 504, 594, 1160, 1965
BmsI GCATC 1 cut(s) 1433
BmtI GCTAGC 1 cut(s) 2153
BmuI ACTGGG 4 cut(s) 504, 594, 1160, 1965
BoxI GACNNNNGTC 1 cut(s) 1022
BpiI GAAGAC 4 cut(s) 89, 1127, 1176, 1488
BplI GAGNNNNNCTC 2 cut(s) 1247, 1279
BpmI CTGGAG 4 cut(s) 449, 903, 1427, 2114
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 4 cut(s) 536, 1249, 1781, 2336
BpuMI CCSGG 5 cut(s) 738, 788, 978, 1003, 1977
BsaAI YACGTR 1 cut(s) 1940
BsaHI GRCGYC 2 cut(s) 1137, 2009
BsaI GGTCTC 2 cut(s) 363, 2278
BsaJI CCNNGG 9 cut(s) 492, 597, 1380, 1712, 1737, 1750, 1786, 1816, 2137
BsaWI WCCGGW 2 cut(s) 1530, 1871
BsaXI ACNNNNNCTCC 2 cut(s) 683, 713
Bse118I RCCGGY 3 cut(s) 776, 1037, 1530
Bse1I ACTGG 7 cut(s) 510, 589, 1166, 1453, 1971, 2238, 2270
Bse3DI GCAATG 2 cut(s) 1044, 2298
BseBI CCWGG 3 cut(s) 812, 1382, 2138
BseDI CCNNGG 9 cut(s) 492, 597, 1380, 1712, 1737, 1750, 1786, 1816, 2137
BseGI GGATG 3 cut(s) 2071, 2131, 2187
BseMI GCAATG 2 cut(s) 1044, 2298
BseMII CTCAG 2 cut(s) 152, 518
BseNI ACTGG 7 cut(s) 510, 589, 1166, 1453, 1971, 2238, 2270
BseRI GAGGAG 5 cut(s) 12, 95, 210, 906, 1805
BseSI GKGCMC 3 cut(s) 456, 878, 1982
BseXI GCAGC 4 cut(s) 605, 1287, 1408, 1671
BseYI CCCAGC 4 cut(s) 266, 712, 1049, 1756
Bsh1236I CGCG 5 cut(s) 831, 1067, 1216, 1961, 2172
Bsh1285I CGRYCG 2 cut(s) 1023, 1531
BshFI GGCC 8 cut(s) 255, 454, 1037, 1748, 1854, 1980, 2136, 2358
BshNI GGYRCC 1 cut(s) 1761
BshTI ACCGGT 1 cut(s) 1530
BsiEI CGRYCG 2 cut(s) 1023, 1531
BsiHKAI GWGCWC 2 cut(s) 1265, 1901
BsiHKCI CYCGRG 2 cut(s) 1856, 2027
BslFI GGGAC 3 cut(s) 770, 1634, 2020
BsmAI GTCTC 6 cut(s) 363, 671, 688, 798, 1403, 2278
BsmBI CGTCTC 1 cut(s) 688
BsmFI GGGAC 3 cut(s) 770, 1634, 2020
BsnI GGCC 8 cut(s) 255, 454, 1037, 1748, 1854, 1980, 2136, 2358
Bso31I GGTCTC 2 cut(s) 363, 2278
BsoBI CYCGRG 2 cut(s) 1856, 2027
Bsp120I GGGCCC 2 cut(s) 452, 1978
Bsp1407I TGTACA 3 cut(s) 1080, 1086, 1464
Bsp19I CCATGG 1 cut(s) 1712
Bsp68I TCGCGA 1 cut(s) 1067
BspANI GGCC 8 cut(s) 255, 454, 1037, 1748, 1854, 1980, 2136, 2358
BspCNI CTCAG 2 cut(s) 151, 519
BspFNI CGCG 5 cut(s) 831, 1067, 1216, 1961, 2172
BspLI GGNNCC 7 cut(s) 161, 454, 1763, 1833, 1969, 1980, 2226
BspMI ACCTGC 1 cut(s) 209
BspOI GCTAGC 1 cut(s) 2153
BspPI GGATC 5 cut(s) 283, 618, 643, 988, 2156
BspQI GCTCTTC 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 1761
BspTNI GGTCTC 2 cut(s) 363, 2278
BsrDI GCAATG 2 cut(s) 1044, 2298
BsrFI RCCGGY 3 cut(s) 776, 1037, 1530
BsrGI TGTACA 3 cut(s) 1080, 1086, 1464
BsrI ACTGG 7 cut(s) 510, 589, 1166, 1453, 1971, 2238, 2270
BssAI RCCGGY 3 cut(s) 776, 1037, 1530
BssECI CCNNGG 9 cut(s) 492, 597, 1380, 1712, 1737, 1750, 1786, 1816, 2137
BssNI GRCGYC 2 cut(s) 1137, 2009
BssSI CACGAG 1 cut(s) 1769
BssT1I CCWWGG 3 cut(s) 492, 1712, 1737
Bst2BI CACGAG 1 cut(s) 1769
Bst2UI CCWGG 3 cut(s) 812, 1382, 2138
Bst4CI ACNGT 5 cut(s) 95, 369, 541, 665, 2258
Bst6I CTCTTC 5 cut(s) 30, 63, 79, 366, 2246
BstACI GRCGYC 2 cut(s) 1137, 2009
BstAUI TGTACA 3 cut(s) 1080, 1086, 1464
BstBAI YACGTR 1 cut(s) 1940
BstC8I GCNNGC 3 cut(s) 1039, 1517, 2151
BstDEI CTNAG 2 cut(s) 138, 527
BstDSI CCRYGG 4 cut(s) 597, 1712, 1750, 1786
BstEII GGTNACC 1 cut(s) 1375
BstF5I GGATG 3 cut(s) 2071, 2131, 2187
BstFNI CGCG 5 cut(s) 831, 1067, 1216, 1961, 2172
BstH2I RGCGCY 1 cut(s) 1824
BstHHI GCGC 6 cut(s) 206, 277, 707, 1823, 2026, 2174
BstMAI GTCTC 6 cut(s) 363, 671, 688, 798, 1403, 2278
BstMCI CGRYCG 2 cut(s) 1023, 1531
BstNI CCWGG 3 cut(s) 812, 1382, 2138
BstNSI RCATGY 1 cut(s) 1087
BstPAI GACNNNNGTC 1 cut(s) 1022
BstPI GGTNACC 1 cut(s) 1375
BstSCI CCNGG 8 cut(s) 736, 786, 810, 976, 1001, 1380, 1975, 2136
BstSLI GKGCMC 3 cut(s) 456, 878, 1982
BstUI CGCG 5 cut(s) 831, 1067, 1216, 1961, 2172
BstV1I GCAGC 4 cut(s) 605, 1287, 1408, 1671
BstV2I GAAGAC 4 cut(s) 89, 1127, 1176, 1488
BstX2I RGATCY 2 cut(s) 610, 635
BstXI CCANNNNNNTGG 3 cut(s) 186, 1744, 2144
BstYI RGATCY 2 cut(s) 610, 635
BsuRI GGCC 8 cut(s) 255, 454, 1037, 1748, 1854, 1980, 2136, 2358
BtgI CCRYGG 4 cut(s) 597, 1712, 1750, 1786
BtgZI GCGATG 1 cut(s) 1082
BtrI CACGTC 1 cut(s) 1783
BtsCI GGATG 3 cut(s) 2071, 2131, 2187
BtuMI TCGCGA 1 cut(s) 1067
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 3 cut(s) 1039, 1517, 2151
CfoI GCGC 6 cut(s) 206, 277, 707, 1823, 2026, 2174
Cfr10I RCCGGY 3 cut(s) 776, 1037, 1530
Cfr13I GGNCC 9 cut(s) 428, 452, 453, 1576, 1747, 1967, 1978, 1979, 2134
CseI GACGC 1 cut(s) 2253
Csp6I GTAC 6 cut(s) 1081, 1087, 1465, 1762, 2071, 2254
CspAI ACCGGT 1 cut(s) 1530
CspCI CAANNNNNGTGG 4 cut(s) 354, 389, 2042, 2077
CviAII CATG 7 cut(s) 180, 186, 307, 1084, 1713, 2021, 2328
CviQI GTAC 6 cut(s) 1081, 1087, 1465, 1762, 2071, 2254
DdeI CTNAG 2 cut(s) 138, 527
DraIII CACNNNGTG 1 cut(s) 1218
DrdI GACNNNNNNGTC 1 cut(s) 1180
DseDI GACNNNNNNGTC 1 cut(s) 1180
EaeI YGGCCR 2 cut(s) 1035, 2356
Eam1104I CTCTTC 5 cut(s) 30, 63, 79, 366, 2246
EarI CTCTTC 5 cut(s) 30, 63, 79, 366, 2246
EciI GGCGGA 1 cut(s) 2201
Ecl136II GAGCTC 2 cut(s) 1263, 1899
Eco130I CCWWGG 3 cut(s) 492, 1712, 1737
Eco147I AGGCCT 1 cut(s) 255
Eco24I GRGCYC 9 cut(s) 84, 456, 1008, 1265, 1694, 1836, 1901, 1982, 1994
Eco31I GGTCTC 2 cut(s) 363, 2278
Eco47I GGWCC 3 cut(s) 428, 1576, 1967
Eco53kI GAGCTC 2 cut(s) 1263, 1899
Eco57I CTGAAG 1 cut(s) 1169
Eco72I CACGTG 1 cut(s) 1940
Eco88I CYCGRG 2 cut(s) 1856, 2027
Eco91I GGTNACC 1 cut(s) 1375
EcoICRI GAGCTC 2 cut(s) 1263, 1899
EcoO109I RGGNCCY 3 cut(s) 452, 1967, 1979
EcoO65I GGTNACC 1 cut(s) 1375
EcoRII CCWGG 3 cut(s) 810, 1380, 2136
EcoT14I CCWWGG 3 cut(s) 492, 1712, 1737
EcoT38I GRGCYC 9 cut(s) 84, 456, 1008, 1265, 1694, 1836, 1901, 1982, 1994
ErhI CCWWGG 3 cut(s) 492, 1712, 1737
Esp3I CGTCTC 1 cut(s) 688
FaeI CATG 7 cut(s) 183, 189, 310, 1087, 1716, 2024, 2331
FaqI GGGAC 3 cut(s) 770, 1634, 2020
FatI CATG 7 cut(s) 179, 185, 306, 1083, 1712, 2020, 2327
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 2 cut(s) 1527, 1928
FokI GGATG 3 cut(s) 2078, 2138, 2194
FriOI GRGCYC 9 cut(s) 84, 456, 1008, 1265, 1694, 1836, 1901, 1982, 1994
GlaI GCGC 6 cut(s) 205, 276, 706, 1822, 2025, 2173
GsaI CCCAGC 4 cut(s) 270, 716, 1053, 1760
GsuI CTGGAG 4 cut(s) 449, 903, 1427, 2114
HaeII RGCGCY 1 cut(s) 1824
HaeIII GGCC 8 cut(s) 255, 454, 1037, 1748, 1854, 1980, 2136, 2358
HgaI GACGC 1 cut(s) 2253
HhaI GCGC 6 cut(s) 206, 277, 707, 1823, 2026, 2174
Hin1I GRCGYC 2 cut(s) 1137, 2009
Hin1II CATG 7 cut(s) 183, 189, 310, 1087, 1716, 2024, 2331
Hin6I GCGC 6 cut(s) 204, 275, 705, 1821, 2024, 2172
HinP1I GCGC 6 cut(s) 204, 275, 705, 1821, 2024, 2172
HincII GTYRAC 2 cut(s) 1528, 1929
HindII GTYRAC 2 cut(s) 1528, 1929
HphI GGTGA 7 cut(s) 766, 882, 1387, 1856, 1886, 2259, 2325
Hpy166II GTNNAC 8 cut(s) 1212, 1334, 1528, 1579, 1786, 1929, 2071, 2333
Hpy188III TCNNGA 9 cut(s) 224, 553, 805, 1066, 1203, 1546, 1858, 1952, 2315
Hpy8I GTNNAC 8 cut(s) 1212, 1334, 1528, 1579, 1786, 1929, 2071, 2333
Hpy99I CGWCG 9 cut(s) 622, 649, 988, 1021, 1028, 1139, 1699, 1933, 2014
HpyCH4III ACNGT 5 cut(s) 95, 369, 541, 665, 2258
HpyCH4IV ACGT 5 cut(s) 681, 1137, 1782, 1939, 2009
HpyCH4V TGCA 3 cut(s) 593, 905, 1058
HpyF3I CTNAG 2 cut(s) 138, 527
HpySE526I ACGT 5 cut(s) 681, 1137, 1782, 1939, 2009
Hsp92I GRCGYC 2 cut(s) 1137, 2009
Hsp92II CATG 7 cut(s) 183, 189, 310, 1087, 1716, 2024, 2331
HspAI GCGC 6 cut(s) 204, 275, 705, 1821, 2024, 2172
KpnI GGTACC 1 cut(s) 1765
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
Ksp22I TGATCA 1 cut(s) 627
LguI GCTCTTC 1 cut(s) 79
LmnI GCTCC 4 cut(s) 1268, 1303, 1831, 1896
Lsp1109I GCAGC 4 cut(s) 605, 1287, 1408, 1671
LweI GCATC 1 cut(s) 1433
MaeII ACGT 5 cut(s) 681, 1137, 1782, 1939, 2009
MaeIII GTNAC 8 cut(s) 196, 671, 1138, 1171, 1375, 1676, 2031, 2265
MflI RGATCY 2 cut(s) 610, 635
MlsI TGGCCA 1 cut(s) 2358
MluNI TGGCCA 1 cut(s) 2358
MlyI GAGTC 4 cut(s) 255, 800, 1405, 2039
MmeI TCCRAC 5 cut(s) 605, 627, 966, 1006, 2190
Mox20I TGGCCA 1 cut(s) 2358
MroNI GCCGGC 1 cut(s) 1037
MroXI GAANNNNTTC 2 cut(s) 318, 1293
MscI TGGCCA 1 cut(s) 2358
MseI TTAA 4 cut(s) 414, 474, 678, 1419
MslI CAYNNNNRTG 2 cut(s) 184, 1373
Msp20I TGGCCA 1 cut(s) 2358
MspR9I CCNGG 8 cut(s) 738, 788, 812, 978, 1003, 1382, 1977, 2138
MvaI CCWGG 3 cut(s) 812, 1382, 2138
MvnI CGCG 5 cut(s) 831, 1067, 1216, 1961, 2172
NaeI GCCGGC 1 cut(s) 1039
NciI CCSGG 5 cut(s) 738, 788, 978, 1003, 1977
NcoI CCATGG 1 cut(s) 1712
NgoMIV GCCGGC 1 cut(s) 1037
NheI GCTAGC 1 cut(s) 2149
NlaIII CATG 7 cut(s) 183, 189, 310, 1087, 1716, 2024, 2331
NlaIV GGNNCC 7 cut(s) 161, 454, 1763, 1833, 1969, 1980, 2226
NmeAIII GCCGAG 2 cut(s) 751, 1797
NmuCI GTSAC 6 cut(s) 196, 1138, 1171, 1375, 2031, 2265
NruI TCGCGA 1 cut(s) 1067
NspI RCATGY 1 cut(s) 1087
OliI CACNNNNGTG 1 cut(s) 1373
PaeR7I CTCGAG 1 cut(s) 1856
PceI AGGCCT 1 cut(s) 255
PciI ACATGT 1 cut(s) 1083
PciSI GCTCTTC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 4 cut(s) 980, 1029, 1038, 2006
PdiI GCCGGC 1 cut(s) 1039
PdmI GAANNNNTTC 2 cut(s) 318, 1293
PfeI GAWTC 7 cut(s) 622, 696, 1029, 1199, 1268, 1509, 1792
PflFI GACNNNGTC 1 cut(s) 1971
PfoI TCCNGGA 1 cut(s) 786
PinAI ACCGGT 1 cut(s) 1530
Ple19I CGATCG 1 cut(s) 1023
PleI GAGTC 4 cut(s) 254, 799, 1405, 2038
PmaCI CACGTG 1 cut(s) 1940
PmlI CACGTG 1 cut(s) 1940
PpsI GAGTC 4 cut(s) 254, 799, 1405, 2038
Ppu21I YACGTR 1 cut(s) 1940
PpuMI RGGWCCY 1 cut(s) 1967
PscI ACATGT 1 cut(s) 1083
PshAI GACNNNNGTC 1 cut(s) 1022
PsiI TTATAA 1 cut(s) 2365
Psp124BI GAGCTC 2 cut(s) 1265, 1901
Psp5II RGGWCCY 1 cut(s) 1967
Psp6I CCWGG 3 cut(s) 810, 1380, 2136
PspCI CACGTG 1 cut(s) 1940
PspEI GGTNACC 1 cut(s) 1375
PspFI CCCAGC 4 cut(s) 266, 712, 1049, 1756
PspGI CCWGG 3 cut(s) 810, 1380, 2136
PspN4I GGNNCC 7 cut(s) 161, 454, 1763, 1833, 1969, 1980, 2226
PspOMI GGGCCC 2 cut(s) 452, 1978
PspPI GGNCC 9 cut(s) 428, 452, 453, 1576, 1747, 1967, 1978, 1979, 2134
PspPPI RGGWCCY 1 cut(s) 1967
PsuI RGATCY 2 cut(s) 610, 635
PsyI GACNNNGTC 1 cut(s) 1971
PvuI CGATCG 1 cut(s) 1023
RruI TCGCGA 1 cut(s) 1067
RsaI GTAC 6 cut(s) 1082, 1088, 1466, 1763, 2072, 2255
RsaNI GTAC 6 cut(s) 1081, 1087, 1465, 1762, 2071, 2254
RseI CAYNNNNRTG 2 cut(s) 184, 1373
SacI GAGCTC 2 cut(s) 1265, 1901
SalI GTCGAC 2 cut(s) 1526, 1927
SapI GCTCTTC 1 cut(s) 79
SaqAI TTAA 4 cut(s) 414, 474, 678, 1419
Sau96I GGNCC 9 cut(s) 428, 452, 453, 1576, 1747, 1967, 1978, 1979, 2134
SchI GAGTC 4 cut(s) 255, 800, 1405, 2039
ScrFI CCNGG 8 cut(s) 738, 788, 812, 978, 1003, 1382, 1977, 2138
SfaNI GCATC 1 cut(s) 1433
Sfr274I CTCGAG 1 cut(s) 1856
SgrAI CRCCGGYG 1 cut(s) 776
SinI GGWCC 3 cut(s) 428, 1576, 1967
SlaI CTCGAG 1 cut(s) 1856
SmiMI CAYNNNNRTG 2 cut(s) 184, 1373
SmlI CTYRAG 5 cut(s) 551, 1228, 1796, 1856, 2315
SmoI CTYRAG 5 cut(s) 551, 1228, 1796, 1856, 2315
SseBI AGGCCT 1 cut(s) 255
SstI GAGCTC 2 cut(s) 1265, 1901
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 8 cut(s) 736, 786, 810, 976, 1001, 1380, 1975, 2136
StyI CCWWGG 3 cut(s) 492, 1712, 1737
TaaI ACNGT 5 cut(s) 95, 369, 541, 665, 2258
TaiI ACGT 5 cut(s) 684, 1140, 1785, 1942, 2012
TatI WGTACW 4 cut(s) 1080, 1086, 1464, 2070
TauI GCSGC 8 cut(s) 660, 824, 834, 852, 932, 1016, 1134, 1368
TfiI GAWTC 7 cut(s) 622, 696, 1029, 1199, 1268, 1509, 1792
Tru1I TTAA 4 cut(s) 414, 474, 678, 1419
Tru9I TTAA 4 cut(s) 414, 474, 678, 1419
TseFI GTSAC 6 cut(s) 196, 1138, 1171, 1375, 2031, 2265
TseI GCWGC 4 cut(s) 593, 1275, 1396, 1659
Tsp45I GTSAC 6 cut(s) 196, 1138, 1171, 1375, 2031, 2265
TspDTI ATGAA 6 cut(s) 174, 981, 1182, 1638, 2309, 2328
TspGWI ACGGA 5 cut(s) 648, 659, 708, 1190, 1316
Tth111I GACNNNGTC 1 cut(s) 1971
VpaK11BI GGWCC 3 cut(s) 428, 1576, 1967
XapI RAATTY 5 cut(s) 576, 732, 949, 1909, 2321
XceI RCATGY 1 cut(s) 1087
XhoI CTCGAG 1 cut(s) 1856
XmiI GTMKAC 2 cut(s) 1527, 1928
XmnI GAANNNNTTC 2 cut(s) 318, 1293
ZraI GACGTC 2 cut(s) 1138, 2010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.