Rw2G014810

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012508
Physical Location & Seq
Reverse (-)
16893602 .. 16896846
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014810.1

Sequence Viewer

Length: 2361 bp
ATGACCTCCCTAAACCCCACTCCTCTTCTTCTTCTTCTTCTACTTCTCACTCTTCTAGCTCAATGCTCTTCTGGGCTCGACACAGACGGTCTTCTTCTCCTCTCCTTCAAACTCTCTATCCTCCAAGACCCTCAGCGAGTTCTTCAAACTTGGAACCCCTACGATACAACGCCATGTTCATGGCGAGGTGTGACCTGCGCCACAATTCTCACTCCTCCTGAGAATATCTTCTACCCTAGAGTCATAGGCCTAGCTCTGCCCAGCTCTGCGCTTGTGGGTTCGATCCCTGCCAATCTTGGCATGATCGAACACCTTCAAAGCCTCAATCTTTCCGACAATTCCCTCAATGGGTCTCTTCCACAGTCTTTGTTCAACGCAACCGAGCTTCAAGTTTTGGACTTGGCATTCAATCGAATACTTGGTCCGCTACCCGAAACCATTTCAGGGCCCAAAAATCTCCAGCTTCTTAACCTCTCTGATAATGCCTTGGAGGGGAAGATACCCCGTAATCTTGCTTCTCTGAGCAACTTGACTGTGATTTCTCTCAAGAACAATTACTTCTCCGGCGAAATTCCGACTGGGTTTGCAGCCGTGGAGGTTTTGGATCTGTCTTCGAATCTGATCAACGGATCTCTGCCGTCGGATTTCGGCGGCGACAGTCTCCGTTACTTTAACGTCTCCCACAACGGAATCTCCGGCGCAATCCCAGCTAACTTCGCCGAGAAAATTCCCGGTAATGCGACCCTTGATCTTTCCTTCAACAACCTCACCGGCGAAGTCCCGGAATCTCCGGCTTTCTTGAACCAGGAAACGGCGGCGTTTCGCGGCAACCCTGATCTGTGCGGCGAAGCAACCGAGAACAACCCGTGCCCTATTCCTTCTTCACCTTCTTCCAATGCACCCAATTCTCCTCCAGCTTTTGCCGCAATTCCCAGAACGAGAAATTCCACCAACGCAGATGAAACTTCGCCTGGATCGTCGGAAAAATCCCAATCCGGGCTCAAAGCGGCGACGATCGTCGGAATCGTGGCCGGCGACATTGCTGGGATTGCAATTATCGCGATGGTGTTCTTGTACATGTACAGATTGAGGAAGAAAAAGAAGGAGAAGGAGAAGACAACAAGCGGCGACGTCACTACGCTGAAGAAAGAAGCCAACCCAGTAGTGACGGATTGGTCTTCTTCTTCATCAGAATCAAGAGGGTTCACGCGGTGGTCTTGCTTGAGAAACAAGAAGGGCGAACCAGACGAGCAGAGCTCCGATTCCAGCAGCTCTTACAGCGAAGAAGTTCAAACGGAGCAGAATGGTCAGAAGGGTAGTGAGAGTAAACGGCAAACAGAGCAAGAACAGAGCAAAGGTGGCACATTGGTGACCCTGGACGGCGAAAAGCAGCTAGAGCTCGAGACTCTGCTTAAAGCATCAGCTTACATTTTGGGTGCTACTGGTTCTAGTATAATGTACAAGGCGGTTTTGGAAGACGGGAGTAGTCTAGCGGTTCGGAGAATTGGCGAGCATAGTGTCGACCGGTTCAAGGATTTCGAGAACCAAATCCGGCTAGTAGCGAAATTGGTCCACCCAAATCTGGTTCGAGTTCGTGGGTTCTTTTGGGGAGTTGATGAAAAGCTCATCATCTATGATTTCGTCCCAAATGGCAGCCTCGCCAATGCTCGTTACAGGAAGGTGGGCTCGTCGCCTTGTCATCTACCATGGGAAGCGAGGTTGAGGATAGCCAAGGGGGTGGCCCGTGGGCTGGGGTACCTCCACGAGAAGAAGCACGTCCACGGGAATCTCAAGCCCACCAACATTCTCCTCGGCGCCGATATGGAGCCCAAAGTCGGTGATTTTGGCCTCGAGAGGCTCTTGTCCGGTGATACGAGCTACAAAATAGGGAGCTCGACTAGGAATTTTGGAAGCAAGAGGTCGACGGCTTCACGTGATAGCTTTCAAGATTTCGCGTTAGGGCCCAGTCCGGGCCCTAGTCCGAGCCCTAGCTCGATGGGGACGTCGCCCTATCATGCGCCCGAGTCGCTTCGGAGCCTAAAGCCCCACCCCAAGTGGGATGTGTACTCATTTGGGGTCATATTGCTGGAGTTGATAACGGGAAAAGTTGTGGTTGTGGATGAGTTGGGCCAGGGTCTTGGGCTAGCCGTTGATGATCCGAACCGCGCTTTTCGGATGGCGGATATGGCGATTAGAGGCGAGTTGGAGGGCAAAGAGGAACCCTTACTGGCTCTCTTCAAGTTAGGGTACAGTTGTGCGTCACCAGTTCCACAAAAGAGACCAGCAATGAAAGAAGCCCTACAAGTTCTTGAGAAATTTCCATGTTCACCTTCATCATCATATTATTATGGCCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

786

Amino Acids

84.93

Weight (kDa)

6.33

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 5.2e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 128 - 186 4.6e-08 Leucine rich repeat
Pkinase PF00069 478 - 768 5e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 493 - 630 6.2e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2359
AasI GACNNNNNNGTC 1 cut(s) 1174
AatII GACGTC 2 cut(s) 1134, 2006
Acc36I ACCTGC 1 cut(s) 203
Acc65I GGTACC 1 cut(s) 1755
AccB1I GGYRCC 2 cut(s) 1755, 1814
AccI GTMKAC 2 cut(s) 1521, 1922
AccII CGCG 5 cut(s) 825, 1061, 1210, 1955, 2166
AclWI GGATC 5 cut(s) 277, 612, 637, 982, 2150
AcoI YGGCCR 2 cut(s) 1029, 2350
AcsI RAATTY 5 cut(s) 570, 726, 943, 1903, 2315
AcuI CTGAAG 1 cut(s) 1163
AcvI CACGTG 1 cut(s) 1934
AcyI GRCGYC 3 cut(s) 1131, 1815, 2003
AdeI CACNNNGTG 1 cut(s) 1212
AfaI GTAC 6 cut(s) 1076, 1082, 1460, 1757, 2066, 2249
AflIII ACRYGT 1 cut(s) 1077
AgeI ACCGGT 1 cut(s) 1524
AjiI CACGTC 1 cut(s) 1777
AjnI CCWGG 4 cut(s) 804, 970, 1374, 2130
AleI CACNNNNGTG 1 cut(s) 1367
Alw21I GWGCWC 3 cut(s) 1259, 1401, 1895
Alw26I GTCTC 5 cut(s) 357, 665, 682, 1397, 2272
AlwI GGATC 5 cut(s) 277, 612, 637, 982, 2150
Ama87I CYCGRG 3 cut(s) 1400, 1850, 2021
AoxI GGCC 9 cut(s) 247, 446, 1029, 1740, 1846, 1961, 1972, 2128, 2350
ApaI GGGCCC 3 cut(s) 450, 1965, 1976
ApeKI GCWGC 4 cut(s) 587, 1269, 1390, 1653
ApoI RAATTY 5 cut(s) 570, 726, 943, 1903, 2315
ArsI GACNNNNNNTTYG 2 cut(s) 406, 438
AsiGI ACCGGT 1 cut(s) 1524
Asp700I GAANNNNTTC 3 cut(s) 227, 312, 1287
Asp718I GGTACC 1 cut(s) 1755
AspLEI GCGC 6 cut(s) 200, 271, 701, 1817, 2020, 2168
AsuC2I CCSGG 4 cut(s) 732, 782, 997, 1971
AsuHPI GGTGA 7 cut(s) 760, 876, 1381, 1850, 1880, 2253, 2319
AsuII TTCGAA 1 cut(s) 614
AsuNHI GCTAGC 1 cut(s) 2143
AvaI CYCGRG 3 cut(s) 1400, 1850, 2021
AvaII GGWCC 2 cut(s) 422, 1570
BaeGI GKGCMC 4 cut(s) 450, 872, 1965, 1976
BaeI ACNNNNGTAYC 2 cut(s) 2239, 2272
BalI TGGCCA 1 cut(s) 2352
BanI GGYRCC 2 cut(s) 1755, 1814
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BauI CACGAG 1 cut(s) 1763
BbrPI CACGTG 1 cut(s) 1934
BbsI GAAGAC 5 cut(s) 83, 603, 1121, 1170, 1482
Bbv12I GWGCWC 3 cut(s) 1259, 1401, 1895
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 4 cut(s) 599, 1281, 1402, 1665
BccI CCATC 3 cut(s) 1057, 1990, 2170
BceAI ACGGC 7 cut(s) 575, 622, 828, 1346, 1396, 1941, 2132
BciT130I CCWGG 4 cut(s) 806, 972, 1376, 2132
BclI TGATCA 1 cut(s) 621
BcnI CCSGG 4 cut(s) 732, 782, 997, 1971
BcoDI GTCTC 5 cut(s) 357, 665, 682, 1397, 2272
BfoI RGCGCY 1 cut(s) 1818
BfuAI ACCTGC 1 cut(s) 203
Bme1390I CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 1971, 2132
Bme18I GGWCC 2 cut(s) 422, 1570
BmeT110I CYCGRG 3 cut(s) 1400, 1850, 2021
BmgBI CACGTC 1 cut(s) 1777
BmiI GGNNCC 9 cut(s) 155, 448, 1757, 1816, 1827, 1963, 1974, 2036, 2220
BmrFI CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 1971, 2132
BmrI ACTGGG 3 cut(s) 588, 1154, 1959
BmsI GCATC 1 cut(s) 1427
BmtI GCTAGC 1 cut(s) 2147
BmuI ACTGGG 3 cut(s) 588, 1154, 1959
BoxI GACNNNNGTC 1 cut(s) 1016
BpiI GAAGAC 5 cut(s) 83, 603, 1121, 1170, 1482
BplI GAGNNNNNCTC 2 cut(s) 1241, 1273
BpmI CTGGAG 3 cut(s) 443, 897, 2108
Bpu10I CCTNAGC 1 cut(s) 132
Bpu14I TTCGAA 1 cut(s) 614
BpuEI CTTGAG 4 cut(s) 530, 1243, 1775, 2330
BpuMI CCSGG 4 cut(s) 732, 782, 997, 1971
BsaAI YACGTR 1 cut(s) 1934
BsaHI GRCGYC 3 cut(s) 1131, 1815, 2003
BsaI GGTCTC 2 cut(s) 357, 2272
BsaJI CCNNGG 9 cut(s) 486, 591, 1374, 1706, 1731, 1744, 1780, 1810, 2131
BsaWI WCCGGW 2 cut(s) 1524, 1865
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bse118I RCCGGY 3 cut(s) 770, 1031, 1524
Bse1I ACTGG 6 cut(s) 583, 1160, 1447, 1965, 2232, 2264
Bse3DI GCAATG 2 cut(s) 1038, 2292
BseBI CCWGG 4 cut(s) 806, 972, 1376, 2132
BseDI CCNNGG 9 cut(s) 486, 591, 1374, 1706, 1731, 1744, 1780, 1810, 2131
BseGI GGATG 3 cut(s) 2065, 2125, 2181
BseMI GCAATG 2 cut(s) 1038, 2292
BseMII CTCAG 3 cut(s) 146, 210, 512
BseNI ACTGG 6 cut(s) 583, 1160, 1447, 1965, 2232, 2264
BseRI GAGGAG 5 cut(s) 12, 89, 204, 900, 1799
BseSI GKGCMC 4 cut(s) 450, 872, 1965, 1976
BseXI GCAGC 4 cut(s) 599, 1281, 1402, 1665
BseYI CCCAGC 4 cut(s) 260, 706, 1043, 1750
Bsh1236I CGCG 5 cut(s) 825, 1061, 1210, 1955, 2166
Bsh1285I CGRYCG 2 cut(s) 1017, 1525
BshFI GGCC 9 cut(s) 249, 448, 1031, 1742, 1848, 1963, 1974, 2130, 2352
BshNI GGYRCC 2 cut(s) 1755, 1814
BshTI ACCGGT 1 cut(s) 1524
BsiEI CGRYCG 2 cut(s) 1017, 1525
BsiHKAI GWGCWC 3 cut(s) 1259, 1401, 1895
BsiHKCI CYCGRG 3 cut(s) 1400, 1850, 2021
BslFI GGGAC 3 cut(s) 764, 1628, 2014
BsmAI GTCTC 5 cut(s) 357, 665, 682, 1397, 2272
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 3 cut(s) 764, 1628, 2014
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 9 cut(s) 249, 448, 1031, 1742, 1848, 1963, 1974, 2130, 2352
Bso31I GGTCTC 2 cut(s) 357, 2272
BsoBI CYCGRG 3 cut(s) 1400, 1850, 2021
Bsp119I TTCGAA 1 cut(s) 614
Bsp120I GGGCCC 3 cut(s) 446, 1961, 1972
Bsp1407I TGTACA 3 cut(s) 1074, 1080, 1458
Bsp19I CCATGG 1 cut(s) 1706
Bsp68I TCGCGA 1 cut(s) 1061
BspANI GGCC 9 cut(s) 249, 448, 1031, 1742, 1848, 1963, 1974, 2130, 2352
BspCNI CTCAG 3 cut(s) 145, 211, 513
BspFNI CGCG 5 cut(s) 825, 1061, 1210, 1955, 2166
BspLI GGNNCC 9 cut(s) 155, 448, 1757, 1816, 1827, 1963, 1974, 2036, 2220
BspMI ACCTGC 1 cut(s) 203
BspOI GCTAGC 1 cut(s) 2147
BspPI GGATC 5 cut(s) 277, 612, 637, 982, 2150
BspQI GCTCTTC 1 cut(s) 73
BspT104I TTCGAA 1 cut(s) 614
BspT107I GGYRCC 2 cut(s) 1755, 1814
BspTNI GGTCTC 2 cut(s) 357, 2272
BsrDI GCAATG 2 cut(s) 1038, 2292
BsrFI RCCGGY 3 cut(s) 770, 1031, 1524
BsrGI TGTACA 3 cut(s) 1074, 1080, 1458
BsrI ACTGG 6 cut(s) 583, 1160, 1447, 1965, 2232, 2264
BssAI RCCGGY 3 cut(s) 770, 1031, 1524
BssECI CCNNGG 9 cut(s) 486, 591, 1374, 1706, 1731, 1744, 1780, 1810, 2131
BssNI GRCGYC 3 cut(s) 1131, 1815, 2003
BssSI CACGAG 1 cut(s) 1763
BssT1I CCWWGG 3 cut(s) 486, 1706, 1731
Bst2BI CACGAG 1 cut(s) 1763
Bst2UI CCWGG 4 cut(s) 806, 972, 1376, 2132
Bst4CI ACNGT 5 cut(s) 89, 363, 535, 659, 2252
Bst6I CTCTTC 5 cut(s) 30, 57, 73, 360, 2240
BstACI GRCGYC 3 cut(s) 1131, 1815, 2003
BstAUI TGTACA 3 cut(s) 1074, 1080, 1458
BstBAI YACGTR 1 cut(s) 1934
BstBI TTCGAA 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 1033, 1511, 2145
BstDEI CTNAG 3 cut(s) 132, 219, 521
BstDSI CCRYGG 4 cut(s) 591, 1706, 1744, 1780
BstEII GGTNACC 1 cut(s) 1369
BstF5I GGATG 3 cut(s) 2065, 2125, 2181
BstFNI CGCG 5 cut(s) 825, 1061, 1210, 1955, 2166
BstH2I RGCGCY 1 cut(s) 1818
BstHHI GCGC 6 cut(s) 200, 271, 701, 1817, 2020, 2168
BstMAI GTCTC 5 cut(s) 357, 665, 682, 1397, 2272
BstMCI CGRYCG 2 cut(s) 1017, 1525
BstNI CCWGG 4 cut(s) 806, 972, 1376, 2132
BstNSI RCATGY 1 cut(s) 1081
BstPAI GACNNNNGTC 1 cut(s) 1016
BstPI GGTNACC 1 cut(s) 1369
BstSCI CCNGG 8 cut(s) 730, 780, 804, 970, 995, 1374, 1969, 2130
BstSLI GKGCMC 4 cut(s) 450, 872, 1965, 1976
BstUI CGCG 5 cut(s) 825, 1061, 1210, 1955, 2166
BstV1I GCAGC 4 cut(s) 599, 1281, 1402, 1665
BstV2I GAAGAC 5 cut(s) 83, 603, 1121, 1170, 1482
BstX2I RGATCY 2 cut(s) 604, 629
BstXI CCANNNNNNTGG 3 cut(s) 180, 1738, 2138
BstYI RGATCY 2 cut(s) 604, 629
BsuRI GGCC 9 cut(s) 249, 448, 1031, 1742, 1848, 1963, 1974, 2130, 2352
BtgI CCRYGG 4 cut(s) 591, 1706, 1744, 1780
BtgZI GCGATG 1 cut(s) 1076
BtrI CACGTC 1 cut(s) 1777
BtsCI GGATG 3 cut(s) 2065, 2125, 2181
BtuMI TCGCGA 1 cut(s) 1061
BveI ACCTGC 1 cut(s) 203
Cac8I GCNNGC 3 cut(s) 1033, 1511, 2145
CfoI GCGC 6 cut(s) 200, 271, 701, 1817, 2020, 2168
Cfr10I RCCGGY 3 cut(s) 770, 1031, 1524
CseI GACGC 1 cut(s) 2247
Csp6I GTAC 6 cut(s) 1075, 1081, 1459, 1756, 2065, 2248
CspAI ACCGGT 1 cut(s) 1524
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviAII CATG 7 cut(s) 174, 180, 301, 1078, 1707, 2015, 2322
CviQI GTAC 6 cut(s) 1075, 1081, 1459, 1756, 2065, 2248
DdeI CTNAG 3 cut(s) 132, 219, 521
DinI GGCGCC 1 cut(s) 1816
DraIII CACNNNGTG 1 cut(s) 1212
DrdI GACNNNNNNGTC 1 cut(s) 1174
DseDI GACNNNNNNGTC 1 cut(s) 1174
EaeI YGGCCR 2 cut(s) 1029, 2350
Eam1104I CTCTTC 5 cut(s) 30, 57, 73, 360, 2240
EarI CTCTTC 5 cut(s) 30, 57, 73, 360, 2240
EciI GGCGGA 1 cut(s) 2195
Ecl136II GAGCTC 3 cut(s) 1257, 1399, 1893
Eco130I CCWWGG 3 cut(s) 486, 1706, 1731
Eco147I AGGCCT 1 cut(s) 249
Eco31I GGTCTC 2 cut(s) 357, 2272
Eco47I GGWCC 2 cut(s) 422, 1570
Eco53kI GAGCTC 3 cut(s) 1257, 1399, 1893
Eco57I CTGAAG 1 cut(s) 1163
Eco72I CACGTG 1 cut(s) 1934
Eco88I CYCGRG 3 cut(s) 1400, 1850, 2021
Eco91I GGTNACC 1 cut(s) 1369
EcoICRI GAGCTC 3 cut(s) 1257, 1399, 1893
EcoO109I RGGNCCY 3 cut(s) 446, 1961, 1973
EcoO65I GGTNACC 1 cut(s) 1369
EcoRII CCWGG 4 cut(s) 804, 970, 1374, 2130
EcoT14I CCWWGG 3 cut(s) 486, 1706, 1731
EgeI GGCGCC 1 cut(s) 1816
EheI GGCGCC 1 cut(s) 1816
ErhI CCWWGG 3 cut(s) 486, 1706, 1731
Esp3I CGTCTC 1 cut(s) 682
FaeI CATG 7 cut(s) 177, 183, 304, 1081, 1710, 2018, 2325
FaqI GGGAC 3 cut(s) 764, 1628, 2014
FatI CATG 7 cut(s) 173, 179, 300, 1077, 1706, 2014, 2321
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 2 cut(s) 1521, 1922
FokI GGATG 3 cut(s) 2072, 2132, 2188
GlaI GCGC 6 cut(s) 199, 270, 700, 1816, 2019, 2167
GsaI CCCAGC 4 cut(s) 264, 710, 1047, 1754
GsuI CTGGAG 3 cut(s) 443, 897, 2108
HaeII RGCGCY 1 cut(s) 1818
HaeIII GGCC 9 cut(s) 249, 448, 1031, 1742, 1848, 1963, 1974, 2130, 2352
HgaI GACGC 1 cut(s) 2247
HhaI GCGC 6 cut(s) 200, 271, 701, 1817, 2020, 2168
Hin1I GRCGYC 3 cut(s) 1131, 1815, 2003
Hin1II CATG 7 cut(s) 177, 183, 304, 1081, 1710, 2018, 2325
Hin6I GCGC 6 cut(s) 198, 269, 699, 1815, 2018, 2166
HinP1I GCGC 6 cut(s) 198, 269, 699, 1815, 2018, 2166
HincII GTYRAC 2 cut(s) 1522, 1923
HindII GTYRAC 2 cut(s) 1522, 1923
HphI GGTGA 7 cut(s) 760, 876, 1381, 1850, 1880, 2253, 2319
Hpy166II GTNNAC 8 cut(s) 1206, 1328, 1522, 1573, 1780, 1923, 2065, 2327
Hpy8I GTNNAC 8 cut(s) 1206, 1328, 1522, 1573, 1780, 1923, 2065, 2327
Hpy99I CGWCG 8 cut(s) 643, 982, 1015, 1022, 1133, 1693, 1927, 2008
HpyCH4III ACNGT 5 cut(s) 89, 363, 535, 659, 2252
HpyCH4IV ACGT 5 cut(s) 675, 1131, 1776, 1933, 2003
HpyCH4V TGCA 3 cut(s) 587, 899, 1052
HpyF3I CTNAG 3 cut(s) 132, 219, 521
HpySE526I ACGT 5 cut(s) 675, 1131, 1776, 1933, 2003
Hsp92I GRCGYC 3 cut(s) 1131, 1815, 2003
Hsp92II CATG 7 cut(s) 177, 183, 304, 1081, 1710, 2018, 2325
HspAI GCGC 6 cut(s) 198, 269, 699, 1815, 2018, 2166
KasI GGCGCC 1 cut(s) 1814
KpnI GGTACC 1 cut(s) 1759
KroI GCCGGC 1 cut(s) 1031
KroNI GCCGGC 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 621
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 5 cut(s) 1262, 1297, 1825, 1890, 2034
Lsp1109I GCAGC 4 cut(s) 599, 1281, 1402, 1665
LweI GCATC 1 cut(s) 1427
MaeII ACGT 5 cut(s) 675, 1131, 1776, 1933, 2003
MaeIII GTNAC 7 cut(s) 190, 665, 1132, 1165, 1369, 1670, 2259
MflI RGATCY 2 cut(s) 604, 629
MlsI TGGCCA 1 cut(s) 2352
MluNI TGGCCA 1 cut(s) 2352
Mly113I GGCGCC 1 cut(s) 1815
MlyI GAGTC 3 cut(s) 249, 1399, 2033
MmeI TCCRAC 6 cut(s) 357, 599, 621, 960, 1000, 2184
Mox20I TGGCCA 1 cut(s) 2352
MroNI GCCGGC 1 cut(s) 1031
MroXI GAANNNNTTC 3 cut(s) 227, 312, 1287
MscI TGGCCA 1 cut(s) 2352
MseI TTAA 3 cut(s) 468, 672, 1413
MslI CAYNNNNRTG 2 cut(s) 178, 1367
Msp20I TGGCCA 1 cut(s) 2352
MspR9I CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 1971, 2132
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 4 cut(s) 806, 972, 1376, 2132
MvnI CGCG 5 cut(s) 825, 1061, 1210, 1955, 2166
NaeI GCCGGC 1 cut(s) 1033
NarI GGCGCC 1 cut(s) 1815
NciI CCSGG 4 cut(s) 732, 782, 997, 1971
NcoI CCATGG 1 cut(s) 1706
NgoMIV GCCGGC 1 cut(s) 1031
NheI GCTAGC 1 cut(s) 2143
NlaIII CATG 7 cut(s) 177, 183, 304, 1081, 1710, 2018, 2325
NlaIV GGNNCC 9 cut(s) 155, 448, 1757, 1816, 1827, 1963, 1974, 2036, 2220
NmeAIII GCCGAG 2 cut(s) 745, 1791
NmuCI GTSAC 5 cut(s) 190, 1132, 1165, 1369, 2259
NruI TCGCGA 1 cut(s) 1061
NspI RCATGY 1 cut(s) 1081
NspV TTCGAA 1 cut(s) 614
OliI CACNNNNGTG 1 cut(s) 1367
PaeR7I CTCGAG 2 cut(s) 1400, 1850
PceI AGGCCT 1 cut(s) 249
PciI ACATGT 1 cut(s) 1077
PciSI GCTCTTC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 4 cut(s) 974, 1023, 1032, 2000
PctI GAATGC 1 cut(s) 404
PdiI GCCGGC 1 cut(s) 1033
PdmI GAANNNNTTC 3 cut(s) 227, 312, 1287
PfeI GAWTC 7 cut(s) 616, 690, 785, 1023, 1193, 1262, 1786
PfoI TCCNGGA 1 cut(s) 780
PinAI ACCGGT 1 cut(s) 1524
Ple19I CGATCG 1 cut(s) 1017
PleI GAGTC 3 cut(s) 248, 1399, 2032
PluTI GGCGCC 1 cut(s) 1818
PmaCI CACGTG 1 cut(s) 1934
PmlI CACGTG 1 cut(s) 1934
PpsI GAGTC 3 cut(s) 248, 1399, 2032
Ppu21I YACGTR 1 cut(s) 1934
PscI ACATGT 1 cut(s) 1077
PshAI GACNNNNGTC 1 cut(s) 1016
PsiI TTATAA 1 cut(s) 2359
Psp124BI GAGCTC 3 cut(s) 1259, 1401, 1895
Psp6I CCWGG 4 cut(s) 804, 970, 1374, 2130
PspCI CACGTG 1 cut(s) 1934
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 4 cut(s) 260, 706, 1043, 1750
PspGI CCWGG 4 cut(s) 804, 970, 1374, 2130
PspN4I GGNNCC 9 cut(s) 155, 448, 1757, 1816, 1827, 1963, 1974, 2036, 2220
PspOMI GGGCCC 3 cut(s) 446, 1961, 1972
PsuI RGATCY 2 cut(s) 604, 629
PvuI CGATCG 1 cut(s) 1017
RruI TCGCGA 1 cut(s) 1061
RsaI GTAC 6 cut(s) 1076, 1082, 1460, 1757, 2066, 2249
RsaNI GTAC 6 cut(s) 1075, 1081, 1459, 1756, 2065, 2248
RseI CAYNNNNRTG 2 cut(s) 178, 1367
SacI GAGCTC 3 cut(s) 1259, 1401, 1895
SalI GTCGAC 2 cut(s) 1520, 1921
SapI GCTCTTC 1 cut(s) 73
SaqAI TTAA 3 cut(s) 468, 672, 1413
SchI GAGTC 3 cut(s) 249, 1399, 2033
ScrFI CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 1971, 2132
SfaNI GCATC 1 cut(s) 1427
SfoI GGCGCC 1 cut(s) 1816
Sfr274I CTCGAG 2 cut(s) 1400, 1850
SfuI TTCGAA 1 cut(s) 614
SgrAI CRCCGGYG 1 cut(s) 770
SinI GGWCC 2 cut(s) 422, 1570
SlaI CTCGAG 2 cut(s) 1400, 1850
SmiMI CAYNNNNRTG 2 cut(s) 178, 1367
SmlI CTYRAG 6 cut(s) 545, 1222, 1400, 1790, 1850, 2309
SmoI CTYRAG 6 cut(s) 545, 1222, 1400, 1790, 1850, 2309
SseBI AGGCCT 1 cut(s) 249
SspDI GGCGCC 1 cut(s) 1814
SstI GAGCTC 3 cut(s) 1259, 1401, 1895
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 8 cut(s) 730, 780, 804, 970, 995, 1374, 1969, 2130
StyI CCWWGG 3 cut(s) 486, 1706, 1731
TaaI ACNGT 5 cut(s) 89, 363, 535, 659, 2252
TaiI ACGT 5 cut(s) 678, 1134, 1779, 1936, 2006
TatI WGTACW 4 cut(s) 1074, 1080, 1458, 2064
TauI GCSGC 7 cut(s) 654, 818, 828, 846, 926, 1010, 1128
TfiI GAWTC 7 cut(s) 616, 690, 785, 1023, 1193, 1262, 1786
Tru1I TTAA 3 cut(s) 468, 672, 1413
Tru9I TTAA 3 cut(s) 468, 672, 1413
TseFI GTSAC 5 cut(s) 190, 1132, 1165, 1369, 2259
TseI GCWGC 4 cut(s) 587, 1269, 1390, 1653
Tsp45I GTSAC 5 cut(s) 190, 1132, 1165, 1369, 2259
TspDTI ATGAA 6 cut(s) 168, 975, 1176, 1632, 2303, 2322
TspGWI ACGGA 5 cut(s) 642, 653, 702, 1184, 1310
VpaK11BI GGWCC 2 cut(s) 422, 1570
XapI RAATTY 5 cut(s) 570, 726, 943, 1903, 2315
XceI RCATGY 1 cut(s) 1081
XhoI CTCGAG 2 cut(s) 1400, 1850
XmiI GTMKAC 2 cut(s) 1521, 1922
XmnI GAANNNNTTC 3 cut(s) 227, 312, 1287
ZraI GACGTC 2 cut(s) 1132, 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.