MD02G1178600.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012508
Physical Location & Seq
Reverse (-)
15782140 .. 15785397
3258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1178600.v1.1.491

Sequence Viewer

Length: 2346 bp
ATGAGCTCCCAAAGCCTCCATTTTTTCATATGCTTTCTTCTTCTCCTCTCGGTCCAATCTTTTGGTCTCGACAGAGATGGACTTCTGTTACTTTCTTTCAAGTTCTCTATCCTCCGTGACCCTCAGTCCGTTCTTGCCACCTGGAATTCGTGCGACGAGACGCCATGTTCATGGAGAGGCGTCGTTTGCGCGCCGCAGCCGACGAACTCGTCGGAAAACTACCAACGAGTGGTAAGTTTAAATCTTCCCAACTCTCAGCTTCTGGGTTCGATCCCTGCCAAACTTGGCATGATCGAACACCTTCAAAGTCTCGATCTTTCCAACAATTCCCTCAACGGTTCGCTCCCACAGTCGCTCTTCAACGCCACGGAGCTTCGGGTTCTTGATTTGTCGTACAATTTGATCTCCGGGGAGCTGCCGGACACGGTGTCGCAGCTGACCAACCTCCAGAGTCTCAACCTCTCCGACAATGCCTTGGCGGGAAAATTGCCCACCAATCTATCTTCTCTGCACTCTCTAACGAGCATTTCTTTGAAGGATAATTACTTCTCAGGCGGTCTTCCGAGCGGGTTCGAATCCGTTCAGGTTTTGGATCTGTCTTCGAATCTGATCAACGGCTCTCTGCCGTCTGATTTTGGCGGCAACAGTCTGAGTTACTTCAACGTCTCCCACAACGGACTTTCCGGGAAAATCCCGCCTGAATTTTCCGAGAAAATTCCAGGTAACGCCAAAACCGACCTATCTTTCAACAATCTTACCGGCGAAGTCCCGGAAACTCAGGTGTTCTTGAACCAAGAAACTGGGTCTTTTACCGGCAATCCTGATCTGTGTGGCCCACCTACTAAGAACCCATGTCCGATTCCTTCTTCTCCTTCCTCTTCACCCAATGATTCGTCTCCGAATTCTCCTCCGGCATTTGCTGCAATTCCCAGGAACCCAGAAGCCAGTTCGCCGAGATCGAAAAATGGGCATTCGCAACAAAGCCAGACCGGGCTGAGACCAGCCGCCATTGTCGGAATAGTCGCCGGTGACATAGCGGGAATCGGAATCCTCGCTCTCGTTTTCCTGTACATATACAGATTGAAGAAGAAGAAGAGCACCAAGGCCGAGATCACAGCAACGCTCAAGAAAGATGCTAATACAAATTCAACGCCGGTAGTCAACGACTGGTCGTCTTCCTCGTCGGAATCGAAAGGGTTCACCAGATGGTCGTGCCTGAGAAAGAGAGGCGAAGACGAAGACAGCTCCGACACGACAGCCTCCGACTCCGAAGAACATCAGACAGAGCAGAATCCGCAAAAGGGTAACGAAAGCAAACGGCAGCAAGAGCAGGAACAGAGCAAAGGCGGGACCTTGGTGACCGTCGACGGCGCAAAGGAGCTAGAACTCGAGACGCTACTCAAAGCTTCGGCGTACATTCTGGGCGCCACCGGTTCGAGCATAATGTACAAGGCGGTGCTCGAAGACGGAAGCTCGTTGGCGGTTCGGAGGATCGGGGAGCAGAGTGTGGACCGGTTCAAGGATTTCGAGAACCAAATCAAGCTTGTGGCCAAACTGGTCCACCCGAACCTGGTTCGAATTCGTGGGTTCTATTGGGGAGTCGACGAGAAGCTCATCATCTATGATTTCGTTCCAAATGGCAGCCTCGCAAACGCACGTTACAGGAAGGTGGGTTCGTCGCCTTGTCATCTACCGTGGGAAGCGAGGCTCAAGATAGCGAAGGGGGTTGCCCGTGGGCTTGCGTACCTCCACGAAAAGAAGCACGTCCACGGGAATCTCAAGCCGACCAACATTCTCTTGGACACAGACATGGAGCCCCGAATTGGTGATTTTGGACTTGAGAGACTTTTGTCCGGCGACACAAGTTACAAAATCGGAAGCTCGATAAGAAATTTCGGAAGCAAGAGATCAACCGCTTCACGGGATAGCTTTCAGGATTTTGCACTTGGGCCTAGTCCAGGCCCAAGTCCAAGCCCTAGCTCTATGGGTACATCGCCTTATCATTGCCCCGAATCGCTTCGGAGCCTGAAGCCGAACCCAAAGTGGGATGTGTACTCATTTGGGGTGATATTGCTTGAGCTACTAACCGGGAAAGTTGTGGTTATGGATGAGGCAGTGCAGGGTCTTGGGCTTGGAGTGGATGATAGTGGCAGGGCTTTCCGGATGGCAGATATGGCAATCAGAGGTGAATTGGAGGGCAAAGAGGAGGCCTTGTTGTCCTGCTTCAAGTTAGGGTACAATTGTGCGTCACCTGTCCCACAAAAGAGACCACCAATGAAAGAAGCCCTACAAGTCCTTGAGAAATTCCCATCTTCTGCTTCATCATCATATTATTATGCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

782

Amino Acids

84.63

Weight (kDa)

6.36

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 6.9e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 179 8.3e-08 Leucine-rich repeat region
LRR_8 PF13855 124 - 182 1.8e-06 Leucine rich repeat
Pkinase PF00069 474 - 764 6e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 489 - 624 1.1e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 1424
AccB7I CCANNNNNTGG 1 cut(s) 229
AccBSI CCGCTC 1 cut(s) 567
AccI GTMKAC 2 cut(s) 1365, 1602
AccII CGCG 1 cut(s) 191
AccIII TCCGGA 1 cut(s) 2160
AclWI GGATC 3 cut(s) 265, 600, 1499
AcoI YGGCCR 1 cut(s) 1548
AcsI RAATTY 8 cut(s) 145, 701, 714, 901, 1144, 1578, 1891, 2303
AcuI CTGAAG 1 cut(s) 2048
AcyI GRCGYC 3 cut(s) 161, 180, 1425
AfaI GTAC 8 cut(s) 395, 1070, 1415, 1448, 1745, 1990, 2054, 2237
AfiI CCNNNNNNNGG 8 cut(s) 229, 1301, 1519, 1570, 1823, 1920, 1958, 2044
AgeI ACCGGT 2 cut(s) 1430, 1512
AhdI GACNNNNNGTC 3 cut(s) 124, 427, 1171
AjiI CACGTC 1 cut(s) 1765
AjnI CCWGG 5 cut(s) 140, 718, 929, 1569, 1957
AloI GAACNNNNNNTCC 2 cut(s) 1657, 1689
Alw21I GWGCWC 3 cut(s) 8, 1100, 1461
AlwI GGATC 3 cut(s) 265, 600, 1499
AlwNI CAGNNNCTG 1 cut(s) 262
Ama87I CYCGRG 1 cut(s) 1388
Aor13HI TCCGGA 1 cut(s) 2160
AoxI GGCC 6 cut(s) 832, 1104, 1548, 1949, 1960, 2208
ApeKI GCWGC 6 cut(s) 196, 415, 433, 920, 1321, 1641
ApoI RAATTY 8 cut(s) 145, 701, 714, 901, 1144, 1578, 1891, 2303
AsiGI ACCGGT 2 cut(s) 1430, 1512
Asp700I GAANNNNTTC 4 cut(s) 300, 579, 1196, 2016
AspLEI GCGC 4 cut(s) 191, 193, 1373, 1427
AspS9I GGNCC 7 cut(s) 52, 833, 1350, 1510, 1558, 1949, 1961
AsuC2I CCSGG 5 cut(s) 409, 685, 770, 991, 2089
AsuHPI GGTGA 8 cut(s) 873, 1040, 1192, 1369, 1838, 2077, 2198, 2241
AsuII TTCGAA 3 cut(s) 573, 602, 1576
AvaI CYCGRG 1 cut(s) 1388
AvaII GGWCC 4 cut(s) 52, 1350, 1510, 1558
BaeI ACNNNNGTAYC 2 cut(s) 2227, 2260
BalI TGGCCA 1 cut(s) 1550
BanI GGYRCC 1 cut(s) 1424
BanII GRGCYC 2 cut(s) 8, 1818
BarI GAAGNNNNNNTAC 2 cut(s) 527, 559
BbsI GAAGAC 6 cut(s) 551, 591, 1167, 1239, 1245, 1470
Bbv12I GWGCWC 3 cut(s) 8, 1100, 1461
BbvI GCAGC 6 cut(s) 208, 402, 445, 907, 1333, 1653
BccI CCATC 4 cut(s) 71, 1200, 2158, 2317
BceAI ACGGC 4 cut(s) 610, 631, 1334, 1384
BciT130I CCWGG 5 cut(s) 142, 720, 931, 1571, 1959
BclI TGATCA 1 cut(s) 609
BcnI CCSGG 5 cut(s) 409, 685, 770, 991, 2089
BfaI CTAG 3 cut(s) 1382, 1953, 1977
BfoI RGCGCY 1 cut(s) 1428
BisI GCNGC 9 cut(s) 194, 197, 416, 434, 640, 921, 1005, 1322, 1642
BlsI GCNGC 9 cut(s) 195, 198, 417, 435, 641, 922, 1006, 1323, 1643
Bme18I GGWCC 4 cut(s) 52, 1350, 1510, 1558
BmeRI GACNNNNNGTC 3 cut(s) 124, 427, 1171
BmeT110I CYCGRG 1 cut(s) 1388
BmgBI CACGTC 1 cut(s) 1765
BmgT120I GGNCC 7 cut(s) 52, 833, 1350, 1510, 1558, 1949, 1961
BmiI GGNNCC 5 cut(s) 935, 1351, 1426, 1815, 2024
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 1 cut(s) 1123
BmuI ACTGGG 1 cut(s) 810
BoxI GACNNNNGTC 1 cut(s) 1849
BpiI GAAGAC 6 cut(s) 551, 591, 1167, 1239, 1245, 1470
BpmI CTGGAG 1 cut(s) 431
Bpu14I TTCGAA 3 cut(s) 573, 602, 1576
BpuEI CTTGAG 6 cut(s) 1109, 1694, 1763, 1859, 2096, 2318
BpuMI CCSGG 5 cut(s) 409, 685, 770, 991, 2089
BsaHI GRCGYC 3 cut(s) 161, 180, 1425
BsaI GGTCTC 3 cut(s) 71, 991, 2260
BsaJI CCNNGG 9 cut(s) 366, 408, 474, 929, 1101, 1353, 1694, 1732, 1768
BsaWI WCCGGW 3 cut(s) 1430, 1512, 2160
BsaXI ACNNNNNCTCC 2 cut(s) 2198, 2228
Bsc4I CCNNNNNNNGG 8 cut(s) 229, 1301, 1519, 1570, 1823, 1920, 1958, 2044
Bse118I RCCGGY 6 cut(s) 758, 812, 1025, 1153, 1430, 1512
Bse1I ACTGG 4 cut(s) 805, 945, 1172, 1560
Bse3DI GCAATG 1 cut(s) 2002
BseAI TCCGGA 1 cut(s) 2160
BseBI CCWGG 5 cut(s) 142, 720, 931, 1571, 1959
BseDI CCNNGG 9 cut(s) 366, 408, 474, 929, 1101, 1353, 1694, 1732, 1768
BseGI GGATG 4 cut(s) 2053, 2113, 2146, 2169
BseLI CCNNNNNNNGG 8 cut(s) 229, 1301, 1519, 1570, 1823, 1920, 1958, 2044
BseMI GCAATG 1 cut(s) 2002
BseMII CTCAG 7 cut(s) 137, 269, 564, 641, 791, 986, 1208
BseNI ACTGG 4 cut(s) 805, 945, 1172, 1560
BsePI GCGCGC 1 cut(s) 189
BseRI GAGGAG 3 cut(s) 35, 897, 2219
BseXI GCAGC 6 cut(s) 208, 402, 445, 907, 1333, 1653
BsgI GTGCAG 2 cut(s) 494, 2138
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 6 cut(s) 834, 1106, 1550, 1951, 1962, 2210
BshNI GGYRCC 1 cut(s) 1424
BshTI ACCGGT 2 cut(s) 1430, 1512
BsiHKAI GWGCWC 3 cut(s) 8, 1100, 1461
BsiHKCI CYCGRG 1 cut(s) 1388
BslFI GGGAC 3 cut(s) 752, 1363, 2240
BslI CCNNNNNNNGG 8 cut(s) 229, 1301, 1519, 1570, 1823, 1920, 1958, 2044
BsmBI CGTCTC 4 cut(s) 152, 670, 900, 1385
BsmFI GGGAC 3 cut(s) 752, 1363, 2240
BsmI GAATGC 1 cut(s) 970
BsnI GGCC 6 cut(s) 834, 1106, 1550, 1951, 1962, 2210
Bso31I GGTCTC 3 cut(s) 71, 991, 2260
BsoBI CYCGRG 1 cut(s) 1388
Bsp119I TTCGAA 3 cut(s) 573, 602, 1576
Bsp1286I GDGCHC 4 cut(s) 8, 1100, 1461, 1818
Bsp13I TCCGGA 1 cut(s) 2160
Bsp1407I TGTACA 2 cut(s) 1068, 1446
BspANI GGCC 6 cut(s) 834, 1106, 1550, 1951, 1962, 2210
BspCNI CTCAG 7 cut(s) 136, 268, 563, 642, 790, 987, 1209
BspEI TCCGGA 1 cut(s) 2160
BspFNI CGCG 1 cut(s) 191
BspLI GGNNCC 5 cut(s) 935, 1351, 1426, 1815, 2024
BspPI GGATC 3 cut(s) 265, 600, 1499
BspQI GCTCTTC 2 cut(s) 362, 1088
BspT104I TTCGAA 3 cut(s) 573, 602, 1576
BspT107I GGYRCC 1 cut(s) 1424
BspTNI GGTCTC 3 cut(s) 71, 991, 2260
BsrBI CCGCTC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 2002
BsrFI RCCGGY 6 cut(s) 758, 812, 1025, 1153, 1430, 1512
BsrGI TGTACA 2 cut(s) 1068, 1446
BsrI ACTGG 4 cut(s) 805, 945, 1172, 1560
BssAI RCCGGY 6 cut(s) 758, 812, 1025, 1153, 1430, 1512
BssECI CCNNGG 9 cut(s) 366, 408, 474, 929, 1101, 1353, 1694, 1732, 1768
BssHII GCGCGC 1 cut(s) 189
BssNI GRCGYC 3 cut(s) 161, 180, 1425
BssT1I CCWWGG 3 cut(s) 474, 1101, 1353
Bst2UI CCWGG 5 cut(s) 142, 720, 931, 1571, 1959
Bst4CI ACNGT 6 cut(s) 338, 351, 427, 647, 1363, 1695
Bst6I CTCTTC 3 cut(s) 362, 883, 1088
BstACI GRCGYC 3 cut(s) 161, 180, 1425
BstAPI GCANNNNNTGC 1 cut(s) 920
BstAUI TGTACA 2 cut(s) 1068, 1446
BstBI TTCGAA 3 cut(s) 573, 602, 1576
BstC8I GCNNGC 2 cut(s) 191, 1740
BstDEI CTNAG 8 cut(s) 123, 255, 550, 650, 777, 843, 995, 1217
BstDSI CCRYGG 4 cut(s) 366, 1694, 1732, 1768
BstEII GGTNACC 1 cut(s) 1357
BstENI CCTNNNNNAGG 1 cut(s) 1956
BstF5I GGATG 4 cut(s) 2053, 2113, 2146, 2169
BstFNI CGCG 1 cut(s) 191
BstH2I RGCGCY 1 cut(s) 1428
BstHHI GCGC 4 cut(s) 191, 193, 1373, 1427
BstMWI GCNNNNNNNGC 6 cut(s) 12, 186, 920, 1294, 1327, 2174
BstNI CCWGG 5 cut(s) 142, 720, 931, 1571, 1959
BstPAI GACNNNNGTC 1 cut(s) 1849
BstPI GGTNACC 1 cut(s) 1357
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 6 cut(s) 208, 402, 445, 907, 1333, 1653
BstV2I GAAGAC 6 cut(s) 551, 591, 1167, 1239, 1245, 1470
BstX2I RGATCY 1 cut(s) 592
BstXI CCANNNNNNTGG 3 cut(s) 62, 171, 800
BstYI RGATCY 1 cut(s) 592
BsuRI GGCC 6 cut(s) 834, 1106, 1550, 1951, 1962, 2210
BtgI CCRYGG 4 cut(s) 366, 1694, 1732, 1768
BtgZI GCGATG 1 cut(s) 1977
BtrI CACGTC 1 cut(s) 1765
BtsCI GGATG 4 cut(s) 2053, 2113, 2146, 2169
BtsI GCAGTG 1 cut(s) 2121
BtsIMutI CAGTG 1 cut(s) 2121
Cac8I GCNNGC 2 cut(s) 191, 1740
CaiI CAGNNNCTG 1 cut(s) 262
CfoI GCGC 4 cut(s) 191, 193, 1373, 1427
Cfr10I RCCGGY 6 cut(s) 758, 812, 1025, 1153, 1430, 1512
Cfr13I GGNCC 7 cut(s) 52, 833, 1350, 1510, 1558, 1949, 1961
CseI GACGC 4 cut(s) 169, 169, 1402, 2235
CsiI ACCWGGT 1 cut(s) 1569
Csp6I GTAC 8 cut(s) 394, 1069, 1414, 1447, 1744, 1989, 2053, 2236
CspAI ACCGGT 2 cut(s) 1430, 1512
CviAII CATG 5 cut(s) 165, 171, 289, 852, 1810
CviQI GTAC 8 cut(s) 394, 1069, 1414, 1447, 1744, 1989, 2053, 2236
DdeI CTNAG 8 cut(s) 123, 255, 550, 650, 777, 843, 995, 1217
DinI GGCGCC 1 cut(s) 1426
DraI TTTAAA 1 cut(s) 240
DrdI GACNNNNNNGTC 1 cut(s) 208
DriI GACNNNNNGTC 3 cut(s) 124, 427, 1171
DseDI GACNNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 1548
Eam1104I CTCTTC 3 cut(s) 362, 883, 1088
Eam1105I GACNNNNNGTC 3 cut(s) 124, 427, 1171
EarI CTCTTC 3 cut(s) 362, 883, 1088
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 474, 1101, 1353
Eco147I AGGCCT 1 cut(s) 2210
Eco24I GRGCYC 2 cut(s) 8, 1818
Eco31I GGTCTC 3 cut(s) 71, 991, 2260
Eco47I GGWCC 4 cut(s) 52, 1350, 1510, 1558
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 2048
Eco88I CYCGRG 1 cut(s) 1388
Eco91I GGTNACC 1 cut(s) 1357
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 1956
EcoO109I RGGNCCY 1 cut(s) 1350
EcoO65I GGTNACC 1 cut(s) 1357
EcoRI GAATTC 3 cut(s) 145, 901, 1578
EcoRII CCWGG 5 cut(s) 140, 718, 929, 1569, 1957
EcoT14I CCWWGG 3 cut(s) 474, 1101, 1353
EcoT38I GRGCYC 2 cut(s) 8, 1818
EgeI GGCGCC 1 cut(s) 1426
EheI GGCGCC 1 cut(s) 1426
ErhI CCWWGG 3 cut(s) 474, 1101, 1353
Esp3I CGTCTC 4 cut(s) 152, 670, 900, 1385
FaeI CATG 5 cut(s) 168, 174, 292, 855, 1813
FaqI GGGAC 3 cut(s) 752, 1363, 2240
FatI CATG 5 cut(s) 164, 170, 288, 851, 1809
FauI CCCGC 5 cut(s) 472, 560, 702, 1030, 1340
FauNDI CATATG 1 cut(s) 29
FbaI TGATCA 1 cut(s) 609
FblI GTMKAC 2 cut(s) 1365, 1602
Fnu4HI GCNGC 9 cut(s) 194, 197, 416, 434, 640, 921, 1005, 1322, 1642
FokI GGATG 4 cut(s) 2060, 2120, 2153, 2176
FriOI GRGCYC 2 cut(s) 8, 1818
Fsp4HI GCNGC 9 cut(s) 194, 197, 416, 434, 640, 921, 1005, 1322, 1642
FspBI CTAG 3 cut(s) 1382, 1953, 1977
GlaI GCGC 4 cut(s) 190, 192, 1372, 1426
GluI GCNGC 9 cut(s) 194, 197, 416, 434, 640, 921, 1005, 1322, 1642
GsuI CTGGAG 1 cut(s) 431
HaeII RGCGCY 1 cut(s) 1428
HaeIII GGCC 6 cut(s) 834, 1106, 1550, 1951, 1962, 2210
HgaI GACGC 4 cut(s) 169, 169, 1402, 2235
HhaI GCGC 4 cut(s) 191, 193, 1373, 1427
Hin1I GRCGYC 3 cut(s) 161, 180, 1425
Hin1II CATG 5 cut(s) 168, 174, 292, 855, 1813
Hin6I GCGC 4 cut(s) 189, 191, 1371, 1425
HinP1I GCGC 4 cut(s) 189, 191, 1371, 1425
HincII GTYRAC 3 cut(s) 1162, 1366, 1603
HindII GTYRAC 3 cut(s) 1162, 1366, 1603
HindIII AAGCTT 2 cut(s) 1404, 1541
HphI GGTGA 8 cut(s) 873, 1040, 1192, 1369, 1838, 2077, 2198, 2241
Hpy166II GTNNAC 8 cut(s) 1162, 1200, 1366, 1510, 1561, 1603, 1768, 2053
Hpy8I GTNNAC 8 cut(s) 1162, 1200, 1366, 1510, 1561, 1603, 1768, 2053
Hpy99I CGWCG 9 cut(s) 158, 185, 205, 214, 1186, 1367, 1370, 1607, 1681
HpyAV CCTTC 6 cut(s) 311, 529, 873, 882, 1660, 1714
HpyCH4III ACNGT 6 cut(s) 338, 351, 427, 647, 1363, 1695
HpyCH4IV ACGT 3 cut(s) 663, 1657, 1764
HpyCH4V TGCA 4 cut(s) 511, 923, 1943, 2119
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 186, 920, 1294, 1327, 2174
HpyF3I CTNAG 8 cut(s) 123, 255, 550, 650, 777, 843, 995, 1217
HpySE526I ACGT 3 cut(s) 663, 1657, 1764
Hsp92I GRCGYC 3 cut(s) 161, 180, 1425
Hsp92II CATG 5 cut(s) 168, 174, 292, 855, 1813
HspAI GCGC 4 cut(s) 189, 191, 1371, 1425
KasI GGCGCC 1 cut(s) 1424
Kpn2I TCCGGA 1 cut(s) 2160
Ksp22I TGATCA 1 cut(s) 609
LguI GCTCTTC 2 cut(s) 362, 1088
LmnI GCTCC 9 cut(s) 11, 348, 370, 412, 1250, 1378, 1498, 1813, 2022
Lsp1109I GCAGC 6 cut(s) 208, 402, 445, 907, 1333, 1653
LweI GCATC 1 cut(s) 1123
MabI ACCWGGT 1 cut(s) 1569
MaeI CTAG 3 cut(s) 1382, 1953, 1977
MaeII ACGT 3 cut(s) 663, 1657, 1764
MbiI CCGCTC 1 cut(s) 567
MfeI CAATTG 1 cut(s) 2239
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 4 cut(s) 8, 1100, 1461, 1818
MlsI TGGCCA 1 cut(s) 1550
MluNI TGGCCA 1 cut(s) 1550
Mly113I GGCGCC 1 cut(s) 1425
MlyI GAGTC 3 cut(s) 460, 1259, 1608
MmeI TCCRAC 7 cut(s) 192, 345, 489, 994, 1164, 1272, 1287
Mox20I TGGCCA 1 cut(s) 1550
MroI TCCGGA 1 cut(s) 2160
MroXI GAANNNNTTC 4 cut(s) 300, 579, 1196, 2016
MscI TGGCCA 1 cut(s) 1550
MseI TTAA 1 cut(s) 239
MslI CAYNNNNRTG 2 cut(s) 169, 1808
Msp20I TGGCCA 1 cut(s) 1550
MspA1I CMGCKG 1 cut(s) 436
MunI CAATTG 1 cut(s) 2239
Mva1269I GAATGC 1 cut(s) 970
MvaI CCWGG 5 cut(s) 142, 720, 931, 1571, 1959
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 6 cut(s) 12, 186, 920, 1294, 1327, 2174
NarI GGCGCC 1 cut(s) 1425
NciI CCSGG 5 cut(s) 409, 685, 770, 991, 2089
NdeI CATATG 1 cut(s) 29
NlaIII CATG 5 cut(s) 168, 174, 292, 855, 1813
NlaIV GGNNCC 5 cut(s) 935, 1351, 1426, 1815, 2024
NmeAIII GCCGAG 2 cut(s) 978, 1132
NmuCI GTSAC 4 cut(s) 116, 1028, 1357, 2247
NspV TTCGAA 3 cut(s) 573, 602, 1576
PaeR7I CTCGAG 1 cut(s) 1388
PauI GCGCGC 1 cut(s) 189
PceI AGGCCT 1 cut(s) 2210
PciSI GCTCTTC 2 cut(s) 362, 1088
PcsI WCGNNNNNNNCGW 4 cut(s) 732, 956, 1178, 1187
PctI GAATGC 1 cut(s) 970
PdmI GAANNNNTTC 4 cut(s) 300, 579, 1196, 2016
PflMI CCANNNNNTGG 1 cut(s) 229
PfoI TCCNGGA 2 cut(s) 683, 768
PinAI ACCGGT 2 cut(s) 1430, 1512
PkrI GCNGC 9 cut(s) 195, 198, 417, 435, 641, 922, 1006, 1323, 1643
PleI GAGTC 3 cut(s) 459, 1259, 1607
PluTI GGCGCC 1 cut(s) 1428
PpsI GAGTC 3 cut(s) 459, 1259, 1607
PpuMI RGGWCCY 1 cut(s) 1350
PshAI GACNNNNGTC 1 cut(s) 1849
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 1350
Psp6I CCWGG 5 cut(s) 140, 718, 929, 1569, 1957
PspEI GGTNACC 1 cut(s) 1357
PspGI CCWGG 5 cut(s) 140, 718, 929, 1569, 1957
PspN4I GGNNCC 5 cut(s) 935, 1351, 1426, 1815, 2024
PspPI GGNCC 7 cut(s) 52, 833, 1350, 1510, 1558, 1949, 1961
PspPPI RGGWCCY 1 cut(s) 1350
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 592
PteI GCGCGC 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 436
RsaI GTAC 8 cut(s) 395, 1070, 1415, 1448, 1745, 1990, 2054, 2237
RsaNI GTAC 8 cut(s) 394, 1069, 1414, 1447, 1744, 1989, 2053, 2236
RseI CAYNNNNRTG 2 cut(s) 169, 1808
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 2 cut(s) 1364, 1601
SapI GCTCTTC 2 cut(s) 362, 1088
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 9 cut(s) 194, 197, 416, 434, 640, 921, 1005, 1322, 1642
Sau96I GGNCC 7 cut(s) 52, 833, 1350, 1510, 1558, 1949, 1961
SchI GAGTC 3 cut(s) 460, 1259, 1608
SduI GDGCHC 4 cut(s) 8, 1100, 1461, 1818
SexAI ACCWGGT 1 cut(s) 1569
SfaNI GCATC 1 cut(s) 1123
SfoI GGCGCC 1 cut(s) 1426
Sfr274I CTCGAG 1 cut(s) 1388
SfuI TTCGAA 3 cut(s) 573, 602, 1576
SgrAI CRCCGGYG 1 cut(s) 1025
SgrDI CGTCGACG 1 cut(s) 1364
SinI GGWCC 4 cut(s) 52, 1350, 1510, 1558
SlaI CTCGAG 1 cut(s) 1388
SmiMI CAYNNNNRTG 2 cut(s) 169, 1808
SmlI CTYRAG 7 cut(s) 1124, 1388, 1709, 1778, 1838, 2075, 2297
SmoI CTYRAG 7 cut(s) 1124, 1388, 1709, 1778, 1838, 2075, 2297
SseBI AGGCCT 1 cut(s) 2210
SspDI GGCGCC 1 cut(s) 1424
SspMI CTAG 3 cut(s) 1382, 1953, 1977
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 2210
StyI CCWWGG 3 cut(s) 474, 1101, 1353
TaaI ACNGT 6 cut(s) 338, 351, 427, 647, 1363, 1695
TaiI ACGT 3 cut(s) 666, 1660, 1767
TaqII GACCGA 1 cut(s) 40
TatI WGTACW 3 cut(s) 1068, 1446, 2052
TauI GCSGC 3 cut(s) 196, 642, 1007
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TscAI CASTG 1 cut(s) 2121
TseFI GTSAC 4 cut(s) 116, 1028, 1357, 2247
TseI GCWGC 6 cut(s) 196, 415, 433, 920, 1321, 1641
Tsp45I GTSAC 4 cut(s) 116, 1028, 1357, 2247
TspDTI ATGAA 4 cut(s) 16, 159, 2291, 2310
TspGWI ACGGA 6 cut(s) 104, 118, 383, 568, 690, 1482
TspRI CASTG 1 cut(s) 2121
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 4 cut(s) 52, 1350, 1510, 1558
XagI CCTNNNNNAGG 1 cut(s) 1956
XapI RAATTY 8 cut(s) 145, 701, 714, 901, 1144, 1578, 1891, 2303
XcmI CCANNNNNNNNNTGG 1 cut(s) 1795
XhoI CTCGAG 1 cut(s) 1388
XmiI GTMKAC 2 cut(s) 1365, 1602
XmnI GAANNNNTTC 4 cut(s) 300, 579, 1196, 2016
XspI CTAG 3 cut(s) 1382, 1953, 1977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.