Prupe.6G229200_v2.0.a1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0012508
Physical Location & Seq
Forward (+)
23280506 .. 23283858
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G229200.1

Sequence Viewer

Length: 2367 bp
ATGAGCACCAAAAGATACTGCGCCACTCTCCATTTGTGGACATGTCTTCTTCTTCTTCTTTCAGTGCAATCTTTTGGGCTTGACACAGATGGCCTTCTTCTACTTTCCTTCAAATTCTCCATTCTTCATGACCCTCAACGTGTTCTTGAGACCTGGAATTCGCACGACGACACGCCATGTTCATGGAGAGGTGTCGTTTGCAGAGGCGTGTCGTCTGATACGTACCAAAGAGTCATAGGCTTGGCTCTGCCCAACTCTCAGCTTCTGGGTTCAATCCCATCCAATCTTGGCATGATCCAGCACCTTCAAAGTCTCAATCTTTCCAACAATTCTCTCAATGGGTCTCTGCCAACCTCCATATTCAACGCCACGGAGCTTCGAGTTCTTGATTTGTCGTACAATCTCATCTCCGGTGAGCTGCCAGACACCATATCACAGCTCACCAACCTCCAGCTCCTCAACCTCTCCGACAATGCCTTGGCGGGAAACTTACCCACCAATCTCACTTCTCTCCGCAGCCTAACGATCGTTTCTTTGAAGAACAATTACTTCTCTGGTGGTCTTCCAAGCGGGTTCCAGTCCGTACAGGTTTTAGATCTGTCTTCCAATCTGATCAACGGCTCTCTGCCTTCTGATTTCGGCGGCAACAGTCTGAGTTACTTTAACGTCTCCCACAACGGACTTTCCGGGAAAATCCCACCGCTGTTTTCCGAGATAATTCCAGGTAACGCCAAAACCGACCTCTCATTCAACAATTTCAGTGGCGAAGTTCCGGAATCTCAGGTTTTCATGAACCAAGAAACAGAGTCTTTTCTTGGAAACCCTTATCTGTGTGGCCAGCCAACCAAGAACCCATGTCCTATTCCATCTTCACCCTCTTCTTCACCCAATGTTTCTTCACCAAATTCTCCTCCAGCATTTGCTGCAATTCCCAGGACTTTGAATTCATCGAACCCAGAAGCCATTTCACCAAGATCAGAGAAGGGGCGTTCACGAGAGAGCCAAAGCGGGCTTAGACCAGCAGCCATTGTAGGGATCATAGCTGGTGATATAGCAGGGATTGCACTTCTTGCTTTGATTTTCTTGTACATATACAGATTGAAGAAAAAGAAGAAGAGCAACAAGGCTGAGATCACCACAACGCTCAAGAAAGAAGCTAATTCCAATTCTACACCACCAGTCAATGATTGGTCTTCTTCCTCATCAGAATCAAAAGGGTTCACAAGATGGTCTTGTTTGAGAAAGAGAACCGAAGAAGAAGAGAGCTCAGATACCACCGCCTCCGACACTGAAGATCATCAAACGGACCAAAATGCTCAAAAGGGTCAAGAAATCAAACGCCAACAAGAGCAGGAACAGAGCAAAGGCGGGACCTTGGTGACTGTTGACGGTGAAAAGGCGCTTGAGCTCGAGACACTACTCAAAGCTTCAGCTTACATCCTTGGAGCCACTGGTTCAAGCATAATGTACAAGGCGGTTCTTGAAGATGGCAGTTCCCTAGCGGTTCGCAGAATCGGCGAGCACAGTGTGGACCGGTTCAAGGATTTTGAGAACCAAGTGAAGCTGGTGGCCAAGTTAGTCCACCCAAATCTGGTTCGAATTCGTGGGTTCTATTGGGGGGTGGAAGAGAAGCTCATCATCTATGACTTCGTCCCAAATGGCAGCCTCGCAAACGCCCGTTACAGGAAAGTGGGCTCCTCGCCTTGTCATCTACCATGGGAAGCTAGGCTCAGGATAGCGAAAGGCGTAGCCCGTGGGCTTGCGTACCTCCATGAGAAGAAGCACGTCCACGGAAACCTCAAGCCCACCAACATTCTCTTGGACAATGACATGGAGCCCAAAATTGGCGATTTTGGACTCGAGAGACTTTTGTCGGGCGACACTAGCTACAAAATTGGAAGCTCAATCCGAAATTTCGGAAGCAAGAGATCGACCACCTCGCGAGATAGCTTTCAAGATTTTGGGCTTGGGCCTAGTCCGGGCCCAAGTCCGAGCCCTAGTTCCATGGGCGCATCGCCTTACCATGCACCGGAGTCGCTTCGGAGCCTAAAGCCCAACCCAAAGTGGGATGCGTACTCATTTGGGGTGATATTGCTTGAGCTACTAACAGGGAAAGTTGTGATTGTGGATGAGACAGGGCAGGGCCTTGGGCTTGCAGTTGATGACACAAGCAGGGCTTTCAGGATGGCAGATATGGCAATTAGGGCTGAGTTGGAGGGCAAAGAGGAGGCCTTGTTGGCCTGCTTCAAGTTAGGGTATAATTGTGCCTCACCTGTCCCACAAAAGAGACCAGCAATGAAAGAAGCCCTGCAAGTCCTTGAGAAATTCCCATCTTCATCATCATCATCATATTATTATGGCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

789

Amino Acids

85.82

Weight (kDa)

7.92

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1942
AccIII TCCGGA 1 cut(s) 772
AclWI GGATC 2 cut(s) 289, 1043
AcoI YGGCCR 3 cut(s) 835, 1569, 2359
AcsI RAATTY 7 cut(s) 113, 157, 904, 943, 1599, 1912, 2324
AcuI CTGAAG 2 cut(s) 1311, 1413
AdeI CACNNNGTG 1 cut(s) 1528
AfaI GTAC 7 cut(s) 224, 398, 585, 1088, 1469, 1766, 2075
AfiI CCNNNNNNNGG 8 cut(s) 1032, 1540, 1591, 1683, 1844, 1979, 2029, 2065
AflIII ACRYGT 2 cut(s) 41, 139
AgeI ACCGGT 1 cut(s) 1533
AjiI CACGTC 1 cut(s) 1786
AjnI CCWGG 3 cut(s) 152, 721, 932
Alw21I GWGCWC 4 cut(s) 8, 1268, 1410, 1524
Alw26I GTCTC 8 cut(s) 143, 317, 348, 673, 1406, 1858, 2126, 2281
AlwI GGATC 2 cut(s) 289, 1043
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 2 cut(s) 1409, 1859
Aor13HI TCCGGA 1 cut(s) 772
AoxI GGCC 9 cut(s) 91, 835, 1569, 1970, 1981, 2143, 2229, 2238, 2359
ApaI GGGCCC 1 cut(s) 1985
ApeKI GCWGC 5 cut(s) 418, 516, 923, 1022, 1662
ApoI RAATTY 7 cut(s) 113, 157, 904, 943, 1599, 1912, 2324
ArsI GACNNNNNNTTYG 2 cut(s) 142, 174
AsiGI ACCGGT 1 cut(s) 1533
AspLEI GCGC 3 cut(s) 23, 1402, 2012
AspS9I GGNCC 7 cut(s) 1306, 1371, 1531, 1970, 1981, 1982, 2143
AsuC2I CCSGG 2 cut(s) 688, 1980
AsuII TTCGAA 1 cut(s) 1597
AvaI CYCGRG 2 cut(s) 1409, 1859
AvaII GGWCC 3 cut(s) 1306, 1371, 1531
BaeGI GKGCMC 1 cut(s) 1985
BalI TGGCCA 3 cut(s) 837, 1571, 2361
BanII GRGCYC 6 cut(s) 1268, 1410, 1697, 1839, 1985, 1997
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 1 cut(s) 993
BbsI GAAGAC 4 cut(s) 38, 554, 594, 1185
Bbv12I GWGCWC 4 cut(s) 8, 1268, 1410, 1524
BbvI GCAGC 5 cut(s) 405, 528, 910, 1034, 1674
BccI CCATC 7 cut(s) 83, 286, 874, 1221, 1481, 2179, 2338
BceAI ACGGC 1 cut(s) 634
BcgI CGANNNNNNTGC 2 cut(s) 2016, 2050
BciT130I CCWGG 3 cut(s) 154, 723, 934
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 2 cut(s) 688, 1980
BcoDI GTCTC 8 cut(s) 143, 317, 348, 673, 1406, 1858, 2126, 2281
BfaI CTAG 5 cut(s) 1499, 1725, 1884, 1974, 1998
BfoI RGCGCY 1 cut(s) 1403
BglI GCCNNNNNGGC 1 cut(s) 2237
BglII AGATCT 1 cut(s) 595
BisI GCNGC 6 cut(s) 419, 517, 643, 924, 1023, 1663
BlsI GCNGC 6 cut(s) 420, 518, 644, 925, 1024, 1664
Bme1390I CCNGG 5 cut(s) 154, 688, 723, 934, 1980
Bme18I GGWCC 3 cut(s) 1306, 1371, 1531
BmeT110I CYCGRG 2 cut(s) 1409, 1859
BmgBI CACGTC 1 cut(s) 1786
BmgT120I GGNCC 7 cut(s) 1306, 1371, 1531, 1970, 1981, 1982, 2143
BmiI GGNNCC 7 cut(s) 575, 1372, 1447, 1696, 1836, 1983, 2045
BmrFI CCNGG 5 cut(s) 154, 688, 723, 934, 1980
BmsI GCATC 2 cut(s) 2021, 2059
BoxI GACNNNNGTC 1 cut(s) 1870
BpiI GAAGAC 4 cut(s) 38, 554, 594, 1185
BpmI CTGGAG 2 cut(s) 434, 897
Bpu10I CCTNAGC 1 cut(s) 1730
Bpu14I TTCGAA 1 cut(s) 1597
BpuEI CTTGAG 6 cut(s) 167, 1130, 1424, 1784, 2117, 2339
BpuMI CCSGG 2 cut(s) 688, 1980
BsaAI YACGTR 1 cut(s) 222
BsaI GGTCTC 3 cut(s) 143, 348, 2281
BsaWI WCCGGW 4 cut(s) 410, 772, 1533, 2029
BsaXI ACNNNNNCTCC 2 cut(s) 2219, 2249
Bsc4I CCNNNNNNNGG 8 cut(s) 1032, 1540, 1591, 1683, 1844, 1979, 2029, 2065
Bse118I RCCGGY 1 cut(s) 1533
Bse1I ACTGG 3 cut(s) 577, 1178, 1456
Bse3DI GCAATG 1 cut(s) 2301
BseAI TCCGGA 1 cut(s) 772
BseBI CCWGG 3 cut(s) 154, 723, 934
BseGI GGATG 5 cut(s) 278, 1437, 2074, 2134, 2190
BseLI CCNNNNNNNGG 8 cut(s) 1032, 1540, 1591, 1683, 1844, 1979, 2029, 2065
BseMI GCAATG 1 cut(s) 2301
BseMII CTCAG 7 cut(s) 272, 644, 794, 1119, 1281, 1744, 2199
BseNI ACTGG 3 cut(s) 577, 1178, 1456
BseRI GAGGAG 4 cut(s) 446, 900, 1687, 2240
BseSI GKGCMC 1 cut(s) 1985
BseXI GCAGC 5 cut(s) 405, 528, 910, 1034, 1674
Bsh1236I CGCG 1 cut(s) 1942
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 9 cut(s) 93, 837, 1571, 1972, 1983, 2145, 2231, 2240, 2361
BshTI ACCGGT 1 cut(s) 1533
BsiEI CGRYCG 1 cut(s) 528
BsiHKAI GWGCWC 4 cut(s) 8, 1268, 1410, 1524
BsiHKCI CYCGRG 2 cut(s) 1409, 1859
BsiSI CCGG 6 cut(s) 411, 687, 773, 1534, 1979, 2030
BslFI GGGAC 3 cut(s) 1384, 1637, 2261
BslI CCNNNNNNNGG 8 cut(s) 1032, 1540, 1591, 1683, 1844, 1979, 2029, 2065
BsmAI GTCTC 8 cut(s) 143, 317, 348, 673, 1406, 1858, 2126, 2281
BsmBI CGTCTC 1 cut(s) 673
BsmFI GGGAC 3 cut(s) 1384, 1637, 2261
BsnI GGCC 9 cut(s) 93, 837, 1571, 1972, 1983, 2145, 2231, 2240, 2361
Bso31I GGTCTC 3 cut(s) 143, 348, 2281
BsoBI CYCGRG 2 cut(s) 1409, 1859
Bsp119I TTCGAA 1 cut(s) 1597
Bsp120I GGGCCC 1 cut(s) 1981
Bsp1286I GDGCHC 8 cut(s) 8, 1268, 1410, 1524, 1697, 1839, 1985, 1997
Bsp13I TCCGGA 1 cut(s) 772
Bsp1407I TGTACA 2 cut(s) 1086, 1467
Bsp143I GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
Bsp19I CCATGG 2 cut(s) 1715, 2004
Bsp68I TCGCGA 1 cut(s) 1942
BspANI GGCC 9 cut(s) 93, 837, 1571, 1972, 1983, 2145, 2231, 2240, 2361
BspCNI CTCAG 7 cut(s) 271, 645, 793, 1120, 1280, 1743, 2200
BspEI TCCGGA 1 cut(s) 772
BspFNI CGCG 1 cut(s) 1942
BspHI TCATGA 2 cut(s) 127, 789
BspLI GGNNCC 7 cut(s) 575, 1372, 1447, 1696, 1836, 1983, 2045
BspPI GGATC 2 cut(s) 289, 1043
BspQI GCTCTTC 1 cut(s) 1109
BspT104I TTCGAA 1 cut(s) 1597
BspTNI GGTCTC 3 cut(s) 143, 348, 2281
BsrDI GCAATG 1 cut(s) 2301
BsrFI RCCGGY 1 cut(s) 1533
BsrGI TGTACA 2 cut(s) 1086, 1467
BsrI ACTGG 3 cut(s) 577, 1178, 1456
BssAI RCCGGY 1 cut(s) 1533
BssMI GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
BssSI CACGAG 1 cut(s) 993
BssT1I CCWWGG 6 cut(s) 477, 1374, 1441, 1715, 2004, 2146
Bst2BI CACGAG 1 cut(s) 993
Bst2UI CCWGG 3 cut(s) 154, 723, 934
Bst4CI ACNGT 4 cut(s) 650, 1384, 1391, 1526
Bst6I CTCTTC 4 cut(s) 883, 1109, 1254, 1620
BstAPI GCANNNNNTGC 3 cut(s) 923, 1061, 1070
BstAUI TGTACA 2 cut(s) 1086, 1467
BstBAI YACGTR 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 1597
BstC8I GCNNGC 6 cut(s) 839, 1010, 1520, 1761, 2154, 2242
BstDEI CTNAG 8 cut(s) 258, 653, 780, 1013, 1128, 1267, 1730, 2208
BstDSI CCRYGG 5 cut(s) 369, 1715, 1753, 1789, 2004
BstF5I GGATG 5 cut(s) 278, 1437, 2074, 2134, 2190
BstFNI CGCG 1 cut(s) 1942
BstH2I RGCGCY 1 cut(s) 1403
BstHHI GCGC 3 cut(s) 23, 1402, 2012
BstKTI GATC 9 cut(s) 297, 528, 598, 615, 977, 1038, 1134, 1297, 1931
BstMAI GTCTC 8 cut(s) 143, 317, 348, 673, 1406, 1858, 2126, 2281
BstMBI GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 8 cut(s) 923, 1061, 1070, 1515, 1884, 2195, 2204, 2237
BstNI CCWGG 3 cut(s) 154, 723, 934
BstNSI RCATGY 1 cut(s) 45
BstPAI GACNNNNGTC 1 cut(s) 1870
BstSCI CCNGG 5 cut(s) 152, 686, 721, 932, 1978
BstSLI GKGCMC 1 cut(s) 1985
BstSNI TACGTA 1 cut(s) 222
BstUI CGCG 1 cut(s) 1942
BstV1I GCAGC 5 cut(s) 405, 528, 910, 1034, 1674
BstV2I GAAGAC 4 cut(s) 38, 554, 594, 1185
BstX2I RGATCY 1 cut(s) 595
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 1 cut(s) 595
BsuRI GGCC 9 cut(s) 93, 837, 1571, 1972, 1983, 2145, 2231, 2240, 2361
BtgI CCRYGG 5 cut(s) 369, 1715, 1753, 1789, 2004
BtgZI GCGATG 1 cut(s) 1998
BtrI CACGTC 1 cut(s) 1786
BtsCI GGATG 5 cut(s) 278, 1437, 2074, 2134, 2190
BtsIMutI CAGTG 5 cut(s) 69, 766, 1287, 1449, 1531
BtuMI TCGCGA 1 cut(s) 1942
Cac8I GCNNGC 6 cut(s) 839, 1010, 1520, 1761, 2154, 2242
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 2 cut(s) 127, 789
CfoI GCGC 3 cut(s) 23, 1402, 2012
Cfr10I RCCGGY 1 cut(s) 1533
Cfr13I GGNCC 7 cut(s) 1306, 1371, 1531, 1970, 1981, 1982, 2143
Csp6I GTAC 7 cut(s) 223, 397, 584, 1087, 1468, 1765, 2074
CspAI ACCGGT 1 cut(s) 1533
CspCI CAANNNNNGTGG 2 cut(s) 742, 777
CviQI GTAC 7 cut(s) 223, 397, 584, 1087, 1468, 1765, 2074
DdeI CTNAG 8 cut(s) 258, 653, 780, 1013, 1128, 1267, 1730, 2208
DpnI GATC 9 cut(s) 296, 527, 597, 614, 976, 1037, 1133, 1296, 1930
DpnII GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
DraIII CACNNNGTG 1 cut(s) 1528
EaeI YGGCCR 3 cut(s) 835, 1569, 2359
Eam1104I CTCTTC 4 cut(s) 883, 1109, 1254, 1620
EarI CTCTTC 4 cut(s) 883, 1109, 1254, 1620
Ecl136II GAGCTC 2 cut(s) 1266, 1408
Eco105I TACGTA 1 cut(s) 222
Eco130I CCWWGG 6 cut(s) 477, 1374, 1441, 1715, 2004, 2146
Eco147I AGGCCT 1 cut(s) 2231
Eco24I GRGCYC 6 cut(s) 1268, 1410, 1697, 1839, 1985, 1997
Eco31I GGTCTC 3 cut(s) 143, 348, 2281
Eco47I GGWCC 3 cut(s) 1306, 1371, 1531
Eco53kI GAGCTC 2 cut(s) 1266, 1408
Eco57I CTGAAG 2 cut(s) 1311, 1413
Eco88I CYCGRG 2 cut(s) 1409, 1859
EcoICRI GAGCTC 2 cut(s) 1266, 1408
EcoO109I RGGNCCY 2 cut(s) 1371, 2143
EcoRI GAATTC 3 cut(s) 157, 943, 1599
EcoRII CCWGG 3 cut(s) 152, 721, 932
EcoT14I CCWWGG 6 cut(s) 477, 1374, 1441, 1715, 2004, 2146
EcoT38I GRGCYC 6 cut(s) 1268, 1410, 1697, 1839, 1985, 1997
ErhI CCWWGG 6 cut(s) 477, 1374, 1441, 1715, 2004, 2146
Esp3I CGTCTC 1 cut(s) 673
FalI AAGNNNNNCTT 4 cut(s) 1216, 1248, 2161, 2193
FaqI GGGAC 3 cut(s) 1384, 1637, 2261
FauI CCCGC 4 cut(s) 475, 563, 1001, 1361
FbaI TGATCA 1 cut(s) 612
Fnu4HI GCNGC 6 cut(s) 419, 517, 643, 924, 1023, 1663
FokI GGATG 5 cut(s) 265, 1424, 2081, 2141, 2197
FriOI GRGCYC 6 cut(s) 1268, 1410, 1697, 1839, 1985, 1997
Fsp4HI GCNGC 6 cut(s) 419, 517, 643, 924, 1023, 1663
FspBI CTAG 5 cut(s) 1499, 1725, 1884, 1974, 1998
GlaI GCGC 3 cut(s) 22, 1401, 2011
GluI GCNGC 6 cut(s) 419, 517, 643, 924, 1023, 1663
GsuI CTGGAG 2 cut(s) 434, 897
HaeII RGCGCY 1 cut(s) 1403
HaeIII GGCC 9 cut(s) 93, 837, 1571, 1972, 1983, 2145, 2231, 2240, 2361
HapII CCGG 6 cut(s) 411, 687, 773, 1534, 1979, 2030
HhaI GCGC 3 cut(s) 23, 1402, 2012
Hin6I GCGC 3 cut(s) 21, 1400, 2010
HinP1I GCGC 3 cut(s) 21, 1400, 2010
HincII GTYRAC 1 cut(s) 1387
HindII GTYRAC 1 cut(s) 1387
HindIII AAGCTT 1 cut(s) 1425
HinfI GANTC 7 cut(s) 231, 776, 806, 1208, 1512, 1857, 2033
HpaII CCGG 6 cut(s) 411, 687, 773, 1534, 1979, 2030
Hpy166II GTNNAC 7 cut(s) 39, 992, 1221, 1387, 1531, 1582, 1789
Hpy8I GTNNAC 7 cut(s) 39, 992, 1221, 1387, 1531, 1582, 1789
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 5 cut(s) 104, 118, 314, 639, 976
HpyCH4III ACNGT 4 cut(s) 650, 1384, 1391, 1526
HpyCH4IV ACGT 4 cut(s) 139, 221, 666, 1785
HpyCH4V TGCA 7 cut(s) 67, 201, 926, 1064, 2027, 2156, 2311
HpyF10VI GCNNNNNNNGC 8 cut(s) 923, 1061, 1070, 1515, 1884, 2195, 2204, 2237
HpyF3I CTNAG 8 cut(s) 258, 653, 780, 1013, 1128, 1267, 1730, 2208
HpySE526I ACGT 4 cut(s) 139, 221, 666, 1785
HspAI GCGC 3 cut(s) 21, 1400, 2010
Kpn2I TCCGGA 1 cut(s) 772
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
LguI GCTCTTC 1 cut(s) 1109
LmnI GCTCC 6 cut(s) 373, 459, 1445, 1700, 1834, 2043
Lsp1109I GCAGC 5 cut(s) 405, 528, 910, 1034, 1674
LweI GCATC 2 cut(s) 2021, 2059
MaeI CTAG 5 cut(s) 1499, 1725, 1884, 1974, 1998
MaeII ACGT 4 cut(s) 139, 221, 666, 1785
MaeIII GTNAC 4 cut(s) 656, 725, 1378, 1679
MalI GATC 9 cut(s) 296, 527, 597, 614, 976, 1037, 1133, 1296, 1930
MboI GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 8 cut(s) 8, 1268, 1410, 1524, 1697, 1839, 1985, 1997
MlsI TGGCCA 3 cut(s) 837, 1571, 2361
MluNI TGGCCA 3 cut(s) 837, 1571, 2361
MlyI GAGTC 4 cut(s) 240, 815, 1851, 2042
MmeI TCCRAC 4 cut(s) 348, 492, 1308, 2193
Mox20I TGGCCA 3 cut(s) 837, 1571, 2361
MroI TCCGGA 1 cut(s) 772
MscI TGGCCA 3 cut(s) 837, 1571, 2361
MseI TTAA 1 cut(s) 663
MslI CAYNNNNRTG 1 cut(s) 181
Msp20I TGGCCA 3 cut(s) 837, 1571, 2361
MspA1I CMGCKG 1 cut(s) 703
MspI CCGG 6 cut(s) 411, 687, 773, 1534, 1979, 2030
MspR9I CCNGG 5 cut(s) 154, 688, 723, 934, 1980
MvaI CCWGG 3 cut(s) 154, 723, 934
MvnI CGCG 1 cut(s) 1942
MwoI GCNNNNNNNGC 8 cut(s) 923, 1061, 1070, 1515, 1884, 2195, 2204, 2237
NciI CCSGG 2 cut(s) 688, 1980
NcoI CCATGG 2 cut(s) 1715, 2004
NdeII GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
NlaIV GGNNCC 7 cut(s) 575, 1372, 1447, 1696, 1836, 1983, 2045
NmuCI GTSAC 1 cut(s) 1378
NruI TCGCGA 1 cut(s) 1942
NspI RCATGY 1 cut(s) 45
NspV TTCGAA 1 cut(s) 1597
PaeR7I CTCGAG 2 cut(s) 1409, 1859
PagI TCATGA 2 cut(s) 127, 789
PceI AGGCCT 1 cut(s) 2231
PciI ACATGT 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 1109
PcsI WCGNNNNNNNCGW 2 cut(s) 218, 735
PfeI GAWTC 3 cut(s) 776, 1208, 1512
PflFI GACNNNGTC 1 cut(s) 1649
PfoI TCCNGGA 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 1533
PkrI GCNGC 6 cut(s) 420, 518, 644, 925, 1024, 1664
Ple19I CGATCG 1 cut(s) 528
PleI GAGTC 4 cut(s) 239, 814, 1851, 2041
PpsI GAGTC 4 cut(s) 239, 814, 1851, 2041
Ppu21I YACGTR 1 cut(s) 222
PpuMI RGGWCCY 1 cut(s) 1371
PscI ACATGT 1 cut(s) 41
PshAI GACNNNNGTC 1 cut(s) 1870
Psp124BI GAGCTC 2 cut(s) 1268, 1410
Psp5II RGGWCCY 1 cut(s) 1371
Psp6I CCWGG 3 cut(s) 152, 721, 932
PspGI CCWGG 3 cut(s) 152, 721, 932
PspN4I GGNNCC 7 cut(s) 575, 1372, 1447, 1696, 1836, 1983, 2045
PspOMI GGGCCC 1 cut(s) 1981
PspPI GGNCC 7 cut(s) 1306, 1371, 1531, 1970, 1981, 1982, 2143
PspPPI RGGWCCY 1 cut(s) 1371
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 595
PsyI GACNNNGTC 1 cut(s) 1649
PvuI CGATCG 1 cut(s) 528
RruI TCGCGA 1 cut(s) 1942
RsaI GTAC 7 cut(s) 224, 398, 585, 1088, 1469, 1766, 2075
RsaNI GTAC 7 cut(s) 223, 397, 584, 1087, 1468, 1765, 2074
RseI CAYNNNNRTG 1 cut(s) 181
SacI GAGCTC 2 cut(s) 1268, 1410
SapI GCTCTTC 1 cut(s) 1109
SaqAI TTAA 1 cut(s) 663
SatI GCNGC 6 cut(s) 419, 517, 643, 924, 1023, 1663
Sau3AI GATC 9 cut(s) 294, 525, 595, 612, 974, 1035, 1131, 1294, 1928
Sau96I GGNCC 7 cut(s) 1306, 1371, 1531, 1970, 1981, 1982, 2143
SchI GAGTC 4 cut(s) 240, 815, 1851, 2042
ScrFI CCNGG 5 cut(s) 154, 688, 723, 934, 1980
SduI GDGCHC 8 cut(s) 8, 1268, 1410, 1524, 1697, 1839, 1985, 1997
SfaNI GCATC 2 cut(s) 2021, 2059
SfiI GGCCNNNNNGGCC 1 cut(s) 2237
Sfr274I CTCGAG 2 cut(s) 1409, 1859
SfuI TTCGAA 1 cut(s) 1597
SinI GGWCC 3 cut(s) 1306, 1371, 1531
SlaI CTCGAG 2 cut(s) 1409, 1859
SmiMI CAYNNNNRTG 1 cut(s) 181
SmlI CTYRAG 8 cut(s) 146, 1145, 1403, 1409, 1799, 1859, 2096, 2318
SmoI CTYRAG 8 cut(s) 146, 1145, 1403, 1409, 1799, 1859, 2096, 2318
SnaBI TACGTA 1 cut(s) 222
SseBI AGGCCT 1 cut(s) 2231
SspMI CTAG 5 cut(s) 1499, 1725, 1884, 1974, 1998
SstI GAGCTC 2 cut(s) 1268, 1410
StuI AGGCCT 1 cut(s) 2231
StyD4I CCNGG 5 cut(s) 152, 686, 721, 932, 1978
StyI CCWWGG 6 cut(s) 477, 1374, 1441, 1715, 2004, 2146
TaaI ACNGT 4 cut(s) 650, 1384, 1391, 1526
TaiI ACGT 4 cut(s) 142, 224, 669, 1788
TaqI TCGA 6 cut(s) 379, 950, 1410, 1597, 1860, 1931
TatI WGTACW 2 cut(s) 1086, 1467
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 3 cut(s) 776, 1208, 1512
Tru1I TTAA 1 cut(s) 663
Tru9I TTAA 1 cut(s) 663
TscAI CASTG 5 cut(s) 69, 766, 1294, 1456, 1531
TseFI GTSAC 1 cut(s) 1378
TseI GCWGC 5 cut(s) 418, 516, 923, 1022, 1662
Tsp45I GTSAC 1 cut(s) 1378
TspDTI ATGAA 7 cut(s) 116, 171, 778, 806, 936, 2312, 2325
TspGWI ACGGA 5 cut(s) 386, 571, 693, 1319, 1806
TspRI CASTG 5 cut(s) 69, 766, 1294, 1456, 1531
Tth111I GACNNNGTC 1 cut(s) 1649
VpaK11BI GGWCC 3 cut(s) 1306, 1371, 1531
XapI RAATTY 7 cut(s) 113, 157, 904, 943, 1599, 1912, 2324
XceI RCATGY 1 cut(s) 45
XcmI CCANNNNNNNNNTGG 2 cut(s) 1185, 1816
XhoI CTCGAG 2 cut(s) 1409, 1859
XspI CTAG 5 cut(s) 1499, 1725, 1884, 1974, 1998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.