Rroxscaffold_2G00136920

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012508
Physical Location & Seq
Forward (+)
74622551 .. 74625586
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136920.1

Sequence Viewer

Length: 2481 bp
ATGACCTCCCTAAACCCCACTCCTCTTCTTCTTCTTCTTCTACTTCTCACTCTTCTAGCTCAATGCTCTTCTGGGCTCGACACAGATGGTCTTCTTCTCCTCTCCTTCAAACTCTCTATCCTCCAAGACCCTCAGCAAGTTCTTCAAACTTGGAACCCCTACGATACAACACCATGTTCATGGCGAGGTGTGACCTGCGCCACAATTCTCACTCCTCCTGAGAATATCTTCTACCCTAGAGTCATAGGCCTAGCTTTGCCCAGCTCTGCGCTTGTGGGTTCGATCCCTGCCAATCTTGGCATGATCGAGCACCTTCAAAGCCTCAATCTTTCCGACAACTCCCTTAATGGGTCTATTCCACTGTCTCTGTTCAACGCAACCGAGCTTCAAGTTTTAGACTTGGCATTCAATCGATTACTTGGTCCGTTACCAGAAACCATTTCAGGGCCCAAAAAACTCCAGCTTCTTAACCTCTCTGATAATGCCTTGGAGGGGAAGATACCCAGTAATCTTGCTTCTCTGAGCAACTTGACTGTCTTTTCTCTCAAGAACAATTACTTCTCCGGCGAAATTCCGACTGGGTTTGCCGCCGTGGAGGTTTTGGATCTGTCCTCGAATCTGATCAACGGATCTCTGCCGTCGGATTTCGGCGGCGACAGTCTCCGTTACTTTAACGTCTCCCACAACGGACTCTCCGGCGCAATCCCAGCTAACTTCGCCGAGAAAATTCCGGGTAATGCGATCCTTGATCTCTCCTTCAACAACCTCACCGGCGAAGTCCCGGAATCTCCGGCTTTCTTGAACCAGGAAACGGCGGCGTTTCGCGGCAACCCTGATTTGTGCGGCGAAGCAACCGAGAACAACCCGTGCCCTATTCCTTCTTCACCTTCTTCCAATGCACCCAATTCTCCTCCAGCTTTTGCCGCAATTCCCAGAACGAGAAATTCCACCAACGCAGATGAAACTTCGCCGGGATCGTCGGAAAACTCCCAATCCGGGCTCAAAGCGGCGACGATCGTCGGAATCGTGGCCGGCGACATTGCTGGGATTGCAATTATCGCGATGGTGTTCTTGTACATATACAGATTGAGGAAGAAAAAGAAGGAGAAGGAGAAGACAACAAGCGGCGAAGTCACTACGCTGAAGAAAGAAGCCAACCCAGTAGTGACGGATTGGTCTTCTTCTTCATCAGAATCAAGAGGGTTCACGCGGTGGTCTTGCTTGAGAAACAAAAAGGGCGAACCAGAGGAGCAGAGCTCCGATTCCAGCAGCTCTTACAGCGAAGAAGTTCAAACGGAGCAGAATGGTCAGAAGGGTGGTGAGAGTAAACGGCAAACAGAGCAAGAACAGAGCAAAGGCGGCACATTGGTGACCCTGGACGGCGAAAAGCAGCTAGAGCTGGAGACTCTGCTTAAAGCATCAGCTTACATTTTGGGTGCTACTGGTTCTAGTATAATGTACAAGGCGGTTTTGGAAGACGGGAGTAGTCTAGCGGTTCGGAGAATCGGCGAGCATAGTGTCGACCGGTTCAAGGATTTCGAGAACCAAATCCGGCTCGTAGCGAAATTGGTCCACCCAAATCTGGTTCGAGTTCGTGGGTTCTTTTGGGGAGTTGATGAAAAGCTCATCATCTATGATTTCGTCCCAAATGGCAGCCTCGCCAATGCTCGTTACATTTACTCTCTTTCTGGTGCTTCTGATAATGATACTCCGAAGCCAATTATTTCAGGAACATCTAATATGCTATGTTTGCTCTTTGGCTCTTCGTTACTTTCCATATCTGTCTTCTCATTTTTGGAAGGTGGGCTCGTCGCCTTGTCATCTACCATGGGAAGCGAGGTTGAGGATAGCCAAGGGGTGGCCCGTGGGCTAGGGTACCTCCACGAGAAGAAGCACGTCCACGGAAATCTCAAGCCCACTAACATTCTCCTCGGCGCCGATATGGAGCCCAAAATTGGTGATTTTGGACTCGAGAGGCTCTTGTCCGGTGATACGAGCTACAAGATAGGGAGCTCGACTAGAAATTTCGGAAGCAAGAGGTCGACGGCTTCACGTGATAGCTTTCAAGATTTCGCGTTAGGGCCCAGTCCGGGCCCTAGTCCGAGCCCTAGCTCGATGGGGACGTCGCCCTATCATGCGCCCGAGTCACTTCGGAGCCTAAAGCCCCACCCCAAGTGGGATGTGTACTCATTTGGGGTAATATTGCTTGAGTTGATAACGGGAAAAGTTGTGGTTGTGGATGAGTTGGGCCAGGGTCTTGGGCTAGCCGTCGATGATCCGAACCGCGCTTTTCGGATGGCGGATATGGCGATTAGAGGCGAGTTGGAGGGCAAAGAGGAACCCTTACTGGCTCTCTTCAAGTTAGGGTACAGTTGTGCATCACCAGTTCCACAAAAGAGACCAGCAATGAAAGAAGCCCTACAAGTTCTTGATAAATTTCCATGTTCACCTTCATCATCATATTATTATGGCCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

826

Amino Acids

88.71

Weight (kDa)

5.37

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 6.5e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 128 - 186 1.9e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 493 - 670 7.9e-16 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 619 - 808 7.3e-18 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2479
AasI GACNNNNNNGTC 1 cut(s) 1174
AatII GACGTC 1 cut(s) 2126
Acc36I ACCTGC 1 cut(s) 203
Acc65I GGTACC 1 cut(s) 1875
AccB1I GGYRCC 2 cut(s) 1875, 1934
AccB7I CCANNNNNTGG 1 cut(s) 1858
AccI GTMKAC 2 cut(s) 1521, 2042
AccII CGCG 5 cut(s) 825, 1061, 1210, 2075, 2286
AclWI GGATC 6 cut(s) 277, 612, 637, 736, 982, 2270
AcoI YGGCCR 2 cut(s) 1029, 2470
AcsI RAATTY 5 cut(s) 570, 726, 943, 2023, 2435
AcuI CTGAAG 1 cut(s) 1163
AcvI CACGTG 1 cut(s) 2054
AcyI GRCGYC 2 cut(s) 1935, 2123
AdeI CACNNNGTG 1 cut(s) 1212
AfaI GTAC 5 cut(s) 1076, 1460, 1877, 2186, 2369
AgeI ACCGGT 1 cut(s) 1524
AjiI CACGTC 1 cut(s) 1897
AjnI CCWGG 3 cut(s) 804, 1374, 2250
AleI CACNNNNGTG 1 cut(s) 1367
Alw21I GWGCWC 3 cut(s) 312, 1259, 2015
Alw26I GTCTC 5 cut(s) 369, 665, 682, 1397, 2392
AlwI GGATC 6 cut(s) 277, 612, 637, 736, 982, 2270
Ama87I CYCGRG 2 cut(s) 1970, 2141
AoxI GGCC 8 cut(s) 247, 446, 1029, 1860, 2081, 2092, 2248, 2470
ApaI GGGCCC 3 cut(s) 450, 2085, 2096
ApeKI GCWGC 3 cut(s) 1269, 1390, 1653
ApoI RAATTY 5 cut(s) 570, 726, 943, 2023, 2435
AsiGI ACCGGT 1 cut(s) 1524
Asp700I GAANNNNTTC 2 cut(s) 227, 1287
Asp718I GGTACC 1 cut(s) 1875
AspLEI GCGC 6 cut(s) 200, 271, 701, 1937, 2140, 2288
AsuC2I CCSGG 5 cut(s) 732, 782, 972, 997, 2091
AsuHPI GGTGA 8 cut(s) 760, 876, 1331, 1381, 1970, 2000, 2373, 2439
AsuNHI GCTAGC 1 cut(s) 2263
AvaI CYCGRG 2 cut(s) 1970, 2141
AvaII GGWCC 2 cut(s) 422, 1570
BaeGI GKGCMC 4 cut(s) 450, 872, 2085, 2096
BaeI ACNNNNGTAYC 2 cut(s) 2359, 2392
BalI TGGCCA 1 cut(s) 2472
BanI GGYRCC 2 cut(s) 1875, 1934
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BauI CACGAG 1 cut(s) 1883
BbrPI CACGTG 1 cut(s) 2054
BbsI GAAGAC 5 cut(s) 83, 1121, 1170, 1482, 1777
Bbv12I GWGCWC 3 cut(s) 312, 1259, 2015
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 1281, 1402, 1665
BccI CCATC 4 cut(s) 80, 1057, 2110, 2290
BceAI ACGGC 7 cut(s) 575, 622, 828, 1346, 1396, 2061, 2252
BciT130I CCWGG 3 cut(s) 806, 1376, 2252
BclI TGATCA 1 cut(s) 621
BcnI CCSGG 5 cut(s) 732, 782, 972, 997, 2091
BcoDI GTCTC 5 cut(s) 369, 665, 682, 1397, 2392
BfoI RGCGCY 1 cut(s) 1938
BfuAI ACCTGC 1 cut(s) 203
Bme1390I CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 2091, 2252
Bme18I GGWCC 2 cut(s) 422, 1570
BmeT110I CYCGRG 2 cut(s) 1970, 2141
BmgBI CACGTC 1 cut(s) 1897
BmiI GGNNCC 9 cut(s) 155, 448, 1877, 1936, 1947, 2083, 2094, 2156, 2340
BmrFI CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 2091, 2252
BmrI ACTGGG 4 cut(s) 498, 588, 1154, 2079
BmsI GCATC 2 cut(s) 1427, 2387
BmtI GCTAGC 1 cut(s) 2267
BmuI ACTGGG 4 cut(s) 498, 588, 1154, 2079
BoxI GACNNNNGTC 1 cut(s) 1016
BpiI GAAGAC 5 cut(s) 83, 1121, 1170, 1482, 1777
BplI GAGNNNNNCTC 2 cut(s) 1241, 1273
BpmI CTGGAG 3 cut(s) 443, 897, 1421
Bpu10I CCTNAGC 1 cut(s) 132
BpuEI CTTGAG 4 cut(s) 530, 1243, 1895, 2228
BpuMI CCSGG 5 cut(s) 732, 782, 972, 997, 2091
Bsa29I ATCGAT 1 cut(s) 412
BsaAI YACGTR 1 cut(s) 2054
BsaHI GRCGYC 2 cut(s) 1935, 2123
BsaI GGTCTC 1 cut(s) 2392
BsaJI CCNNGG 9 cut(s) 486, 591, 1374, 1827, 1852, 1864, 1900, 1930, 2251
BsaWI WCCGGW 2 cut(s) 1524, 1985
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bse118I RCCGGY 3 cut(s) 770, 1031, 1524
Bse1I ACTGG 7 cut(s) 504, 583, 1160, 1447, 2085, 2352, 2384
Bse3DI GCAATG 2 cut(s) 1038, 2412
BseBI CCWGG 3 cut(s) 806, 1376, 2252
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 9 cut(s) 486, 591, 1374, 1827, 1852, 1864, 1900, 1930, 2251
BseGI GGATG 3 cut(s) 2185, 2245, 2301
BseMI GCAATG 2 cut(s) 1038, 2412
BseMII CTCAG 3 cut(s) 146, 210, 512
BseNI ACTGG 7 cut(s) 504, 583, 1160, 1447, 2085, 2352, 2384
BseRI GAGGAG 6 cut(s) 12, 89, 204, 900, 1262, 1919
BseSI GKGCMC 4 cut(s) 450, 872, 2085, 2096
BseXI GCAGC 3 cut(s) 1281, 1402, 1665
BseYI CCCAGC 3 cut(s) 260, 706, 1043
Bsh1236I CGCG 5 cut(s) 825, 1061, 1210, 2075, 2286
Bsh1285I CGRYCG 2 cut(s) 1017, 1525
BshFI GGCC 8 cut(s) 249, 448, 1031, 1862, 2083, 2094, 2250, 2472
BshNI GGYRCC 2 cut(s) 1875, 1934
BshTI ACCGGT 1 cut(s) 1524
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 2 cut(s) 1017, 1525
BsiHKAI GWGCWC 3 cut(s) 312, 1259, 2015
BsiHKCI CYCGRG 2 cut(s) 1970, 2141
BslFI GGGAC 3 cut(s) 764, 1628, 2134
BsmAI GTCTC 5 cut(s) 369, 665, 682, 1397, 2392
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 3 cut(s) 764, 1628, 2134
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 8 cut(s) 249, 448, 1031, 1862, 2083, 2094, 2250, 2472
Bso31I GGTCTC 1 cut(s) 2392
BsoBI CYCGRG 2 cut(s) 1970, 2141
Bsp120I GGGCCC 3 cut(s) 446, 2081, 2092
Bsp1407I TGTACA 2 cut(s) 1074, 1458
Bsp19I CCATGG 1 cut(s) 1827
Bsp68I TCGCGA 1 cut(s) 1061
BspANI GGCC 8 cut(s) 249, 448, 1031, 1862, 2083, 2094, 2250, 2472
BspCNI CTCAG 3 cut(s) 145, 211, 513
BspDI ATCGAT 1 cut(s) 412
BspFNI CGCG 5 cut(s) 825, 1061, 1210, 2075, 2286
BspLI GGNNCC 9 cut(s) 155, 448, 1877, 1936, 1947, 2083, 2094, 2156, 2340
BspMI ACCTGC 1 cut(s) 203
BspOI GCTAGC 1 cut(s) 2267
BspPI GGATC 6 cut(s) 277, 612, 637, 736, 982, 2270
BspQI GCTCTTC 2 cut(s) 73, 1768
BspT107I GGYRCC 2 cut(s) 1875, 1934
BspTNI GGTCTC 1 cut(s) 2392
BsrDI GCAATG 2 cut(s) 1038, 2412
BsrFI RCCGGY 3 cut(s) 770, 1031, 1524
BsrGI TGTACA 2 cut(s) 1074, 1458
BsrI ACTGG 7 cut(s) 504, 583, 1160, 1447, 2085, 2352, 2384
BssAI RCCGGY 3 cut(s) 770, 1031, 1524
BssECI CCNNGG 9 cut(s) 486, 591, 1374, 1827, 1852, 1864, 1900, 1930, 2251
BssNI GRCGYC 2 cut(s) 1935, 2123
BssSI CACGAG 1 cut(s) 1883
BssT1I CCWWGG 3 cut(s) 486, 1827, 1852
Bst2BI CACGAG 1 cut(s) 1883
Bst2UI CCWGG 3 cut(s) 806, 1376, 2252
Bst4CI ACNGT 4 cut(s) 363, 535, 659, 2372
Bst6I CTCTTC 5 cut(s) 30, 57, 73, 1768, 2360
BstACI GRCGYC 2 cut(s) 1935, 2123
BstAUI TGTACA 2 cut(s) 1074, 1458
BstBAI YACGTR 1 cut(s) 2054
BstC8I GCNNGC 3 cut(s) 1033, 1511, 2265
BstDEI CTNAG 3 cut(s) 132, 219, 521
BstDSI CCRYGG 4 cut(s) 591, 1827, 1864, 1900
BstEII GGTNACC 1 cut(s) 1369
BstF5I GGATG 3 cut(s) 2185, 2245, 2301
BstFNI CGCG 5 cut(s) 825, 1061, 1210, 2075, 2286
BstH2I RGCGCY 1 cut(s) 1938
BstHHI GCGC 6 cut(s) 200, 271, 701, 1937, 2140, 2288
BstMAI GTCTC 5 cut(s) 369, 665, 682, 1397, 2392
BstMCI CGRYCG 2 cut(s) 1017, 1525
BstNI CCWGG 3 cut(s) 806, 1376, 2252
BstPAI GACNNNNGTC 1 cut(s) 1016
BstPI GGTNACC 1 cut(s) 1369
BstSCI CCNGG 8 cut(s) 730, 780, 804, 970, 995, 1374, 2089, 2250
BstSLI GKGCMC 4 cut(s) 450, 872, 2085, 2096
BstUI CGCG 5 cut(s) 825, 1061, 1210, 2075, 2286
BstV1I GCAGC 3 cut(s) 1281, 1402, 1665
BstV2I GAAGAC 5 cut(s) 83, 1121, 1170, 1482, 1777
BstX2I RGATCY 2 cut(s) 604, 629
BstXI CCANNNNNNTGG 2 cut(s) 180, 2258
BstYI RGATCY 2 cut(s) 604, 629
Bsu15I ATCGAT 1 cut(s) 412
BsuRI GGCC 8 cut(s) 249, 448, 1031, 1862, 2083, 2094, 2250, 2472
BsuTUI ATCGAT 1 cut(s) 412
BtgI CCRYGG 4 cut(s) 591, 1827, 1864, 1900
BtgZI GCGATG 1 cut(s) 1076
BtrI CACGTC 1 cut(s) 1897
BtsCI GGATG 3 cut(s) 2185, 2245, 2301
BtsIMutI CAGTG 1 cut(s) 359
BtuMI TCGCGA 1 cut(s) 1061
BveI ACCTGC 1 cut(s) 203
Cac8I GCNNGC 3 cut(s) 1033, 1511, 2265
CfoI GCGC 6 cut(s) 200, 271, 701, 1937, 2140, 2288
Cfr10I RCCGGY 3 cut(s) 770, 1031, 1524
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 5 cut(s) 1075, 1459, 1876, 2185, 2368
CspAI ACCGGT 1 cut(s) 1524
CviAII CATG 6 cut(s) 174, 180, 301, 1828, 2135, 2442
CviQI GTAC 5 cut(s) 1075, 1459, 1876, 2185, 2368
DdeI CTNAG 3 cut(s) 132, 219, 521
DinI GGCGCC 1 cut(s) 1936
DraIII CACNNNGTG 1 cut(s) 1212
DrdI GACNNNNNNGTC 1 cut(s) 1174
DseDI GACNNNNNNGTC 1 cut(s) 1174
EaeI YGGCCR 2 cut(s) 1029, 2470
Eam1104I CTCTTC 5 cut(s) 30, 57, 73, 1768, 2360
EarI CTCTTC 5 cut(s) 30, 57, 73, 1768, 2360
EciI GGCGGA 1 cut(s) 2315
Ecl136II GAGCTC 2 cut(s) 1257, 2013
Eco130I CCWWGG 3 cut(s) 486, 1827, 1852
Eco147I AGGCCT 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 2392
Eco47I GGWCC 2 cut(s) 422, 1570
Eco53kI GAGCTC 2 cut(s) 1257, 2013
Eco57I CTGAAG 1 cut(s) 1163
Eco72I CACGTG 1 cut(s) 2054
Eco88I CYCGRG 2 cut(s) 1970, 2141
Eco91I GGTNACC 1 cut(s) 1369
EcoICRI GAGCTC 2 cut(s) 1257, 2013
EcoO109I RGGNCCY 3 cut(s) 446, 2081, 2093
EcoO65I GGTNACC 1 cut(s) 1369
EcoRII CCWGG 3 cut(s) 804, 1374, 2250
EcoT14I CCWWGG 3 cut(s) 486, 1827, 1852
EgeI GGCGCC 1 cut(s) 1936
EheI GGCGCC 1 cut(s) 1936
ErhI CCWWGG 3 cut(s) 486, 1827, 1852
Esp3I CGTCTC 1 cut(s) 682
FaeI CATG 6 cut(s) 177, 183, 304, 1831, 2138, 2445
FaqI GGGAC 3 cut(s) 764, 1628, 2134
FatI CATG 6 cut(s) 173, 179, 300, 1827, 2134, 2441
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 2 cut(s) 1521, 2042
FokI GGATG 3 cut(s) 2192, 2252, 2308
GlaI GCGC 6 cut(s) 199, 270, 700, 1936, 2139, 2287
GsaI CCCAGC 3 cut(s) 264, 710, 1047
GsuI CTGGAG 3 cut(s) 443, 897, 1421
HaeII RGCGCY 1 cut(s) 1938
HaeIII GGCC 8 cut(s) 249, 448, 1031, 1862, 2083, 2094, 2250, 2472
HhaI GCGC 6 cut(s) 200, 271, 701, 1937, 2140, 2288
Hin1I GRCGYC 2 cut(s) 1935, 2123
Hin1II CATG 6 cut(s) 177, 183, 304, 1831, 2138, 2445
Hin6I GCGC 6 cut(s) 198, 269, 699, 1935, 2138, 2286
HinP1I GCGC 6 cut(s) 198, 269, 699, 1935, 2138, 2286
HincII GTYRAC 2 cut(s) 1522, 2043
HindII GTYRAC 2 cut(s) 1522, 2043
HphI GGTGA 8 cut(s) 760, 876, 1331, 1381, 1970, 2000, 2373, 2439
Hpy166II GTNNAC 8 cut(s) 1206, 1328, 1522, 1573, 1900, 2043, 2185, 2447
Hpy8I GTNNAC 8 cut(s) 1206, 1328, 1522, 1573, 1900, 2043, 2185, 2447
Hpy99I CGWCG 8 cut(s) 643, 982, 1015, 1022, 1814, 2047, 2128, 2273
HpyCH4III ACNGT 4 cut(s) 363, 535, 659, 2372
HpyCH4IV ACGT 4 cut(s) 675, 1896, 2053, 2123
HpyCH4V TGCA 3 cut(s) 899, 1052, 2378
HpyF3I CTNAG 3 cut(s) 132, 219, 521
HpySE526I ACGT 4 cut(s) 675, 1896, 2053, 2123
Hsp92I GRCGYC 2 cut(s) 1935, 2123
Hsp92II CATG 6 cut(s) 177, 183, 304, 1831, 2138, 2445
HspAI GCGC 6 cut(s) 198, 269, 699, 1935, 2138, 2286
KasI GGCGCC 1 cut(s) 1934
KpnI GGTACC 1 cut(s) 1879
KroI GCCGGC 1 cut(s) 1031
KroNI GCCGGC 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 621
LguI GCTCTTC 2 cut(s) 73, 1768
LmnI GCTCC 6 cut(s) 1249, 1262, 1297, 1945, 2010, 2154
Lsp1109I GCAGC 3 cut(s) 1281, 1402, 1665
LweI GCATC 2 cut(s) 1427, 2387
MaeII ACGT 4 cut(s) 675, 1896, 2053, 2123
MaeIII GTNAC 9 cut(s) 190, 426, 665, 1132, 1165, 1369, 1670, 1767, 2145
MflI RGATCY 2 cut(s) 604, 629
MlsI TGGCCA 1 cut(s) 2472
MluNI TGGCCA 1 cut(s) 2472
Mly113I GGCGCC 1 cut(s) 1935
MlyI GAGTC 5 cut(s) 249, 684, 1399, 1962, 2153
MmeI TCCRAC 6 cut(s) 357, 599, 621, 960, 1000, 2304
Mox20I TGGCCA 1 cut(s) 2472
MroNI GCCGGC 1 cut(s) 1031
MroXI GAANNNNTTC 2 cut(s) 227, 1287
MscI TGGCCA 1 cut(s) 2472
MseI TTAA 4 cut(s) 345, 468, 672, 1413
MslI CAYNNNNRTG 2 cut(s) 178, 1367
Msp20I TGGCCA 1 cut(s) 2472
MspR9I CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 2091, 2252
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 3 cut(s) 806, 1376, 2252
MvnI CGCG 5 cut(s) 825, 1061, 1210, 2075, 2286
NaeI GCCGGC 1 cut(s) 1033
NarI GGCGCC 1 cut(s) 1935
NciI CCSGG 5 cut(s) 732, 782, 972, 997, 2091
NcoI CCATGG 1 cut(s) 1827
NgoMIV GCCGGC 1 cut(s) 1031
NheI GCTAGC 1 cut(s) 2263
NlaIII CATG 6 cut(s) 177, 183, 304, 1831, 2138, 2445
NlaIV GGNNCC 9 cut(s) 155, 448, 1877, 1936, 1947, 2083, 2094, 2156, 2340
NmeAIII GCCGAG 2 cut(s) 745, 1911
NmuCI GTSAC 5 cut(s) 190, 1132, 1165, 1369, 2145
NruI TCGCGA 1 cut(s) 1061
OliI CACNNNNGTG 1 cut(s) 1367
PaeR7I CTCGAG 1 cut(s) 1970
PceI AGGCCT 1 cut(s) 249
PciSI GCTCTTC 2 cut(s) 73, 1768
PcsI WCGNNNNNNNCGW 4 cut(s) 974, 1023, 1032, 2120
PctI GAATGC 1 cut(s) 404
PdiI GCCGGC 1 cut(s) 1033
PdmI GAANNNNTTC 2 cut(s) 227, 1287
PfeI GAWTC 6 cut(s) 616, 785, 1023, 1193, 1262, 1503
PflMI CCANNNNNTGG 1 cut(s) 1858
PfoI TCCNGGA 1 cut(s) 780
PinAI ACCGGT 1 cut(s) 1524
Ple19I CGATCG 1 cut(s) 1017
PleI GAGTC 5 cut(s) 248, 684, 1399, 1962, 2152
PluTI GGCGCC 1 cut(s) 1938
PmaCI CACGTG 1 cut(s) 2054
PmlI CACGTG 1 cut(s) 2054
PpsI GAGTC 5 cut(s) 248, 684, 1399, 1962, 2152
Ppu21I YACGTR 1 cut(s) 2054
PshAI GACNNNNGTC 1 cut(s) 1016
PsiI TTATAA 1 cut(s) 2479
Psp124BI GAGCTC 2 cut(s) 1259, 2015
Psp6I CCWGG 3 cut(s) 804, 1374, 2250
PspCI CACGTG 1 cut(s) 2054
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 3 cut(s) 260, 706, 1043
PspGI CCWGG 3 cut(s) 804, 1374, 2250
PspN4I GGNNCC 9 cut(s) 155, 448, 1877, 1936, 1947, 2083, 2094, 2156, 2340
PspOMI GGGCCC 3 cut(s) 446, 2081, 2092
PsuI RGATCY 2 cut(s) 604, 629
PvuI CGATCG 1 cut(s) 1017
RruI TCGCGA 1 cut(s) 1061
RsaI GTAC 5 cut(s) 1076, 1460, 1877, 2186, 2369
RsaNI GTAC 5 cut(s) 1075, 1459, 1876, 2185, 2368
RseI CAYNNNNRTG 2 cut(s) 178, 1367
SacI GAGCTC 2 cut(s) 1259, 2015
SalI GTCGAC 2 cut(s) 1520, 2041
SapI GCTCTTC 2 cut(s) 73, 1768
SaqAI TTAA 4 cut(s) 345, 468, 672, 1413
SchI GAGTC 5 cut(s) 249, 684, 1399, 1962, 2153
ScrFI CCNGG 8 cut(s) 732, 782, 806, 972, 997, 1376, 2091, 2252
SfaNI GCATC 2 cut(s) 1427, 2387
SfoI GGCGCC 1 cut(s) 1936
Sfr274I CTCGAG 1 cut(s) 1970
SgrAI CRCCGGYG 1 cut(s) 770
SinI GGWCC 2 cut(s) 422, 1570
SlaI CTCGAG 1 cut(s) 1970
SmiMI CAYNNNNRTG 2 cut(s) 178, 1367
SmlI CTYRAG 5 cut(s) 545, 1222, 1910, 1970, 2207
SmoI CTYRAG 5 cut(s) 545, 1222, 1910, 1970, 2207
SseBI AGGCCT 1 cut(s) 249
SspDI GGCGCC 1 cut(s) 1934
SspI AATATT 1 cut(s) 2202
SstI GAGCTC 2 cut(s) 1259, 2015
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 8 cut(s) 730, 780, 804, 970, 995, 1374, 2089, 2250
StyI CCWWGG 3 cut(s) 486, 1827, 1852
TaaI ACNGT 4 cut(s) 363, 535, 659, 2372
TaiI ACGT 4 cut(s) 678, 1899, 2056, 2126
TatI WGTACW 3 cut(s) 1074, 1458, 2184
TauI GCSGC 9 cut(s) 590, 654, 818, 828, 846, 926, 1010, 1128, 1362
TfiI GAWTC 6 cut(s) 616, 785, 1023, 1193, 1262, 1503
Tru1I TTAA 4 cut(s) 345, 468, 672, 1413
Tru9I TTAA 4 cut(s) 345, 468, 672, 1413
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 5 cut(s) 190, 1132, 1165, 1369, 2145
TseI GCWGC 3 cut(s) 1269, 1390, 1653
Tsp45I GTSAC 5 cut(s) 190, 1132, 1165, 1369, 2145
TspDTI ATGAA 6 cut(s) 168, 975, 1176, 1632, 2423, 2442
TspGWI ACGGA 7 cut(s) 414, 642, 653, 702, 1184, 1310, 1917
TspRI CASTG 1 cut(s) 366
Van91I CCANNNNNTGG 1 cut(s) 1858
VpaK11BI GGWCC 2 cut(s) 422, 1570
XapI RAATTY 5 cut(s) 570, 726, 943, 2023, 2435
XhoI CTCGAG 1 cut(s) 1970
XmiI GTMKAC 2 cut(s) 1521, 2042
XmnI GAANNNNTTC 2 cut(s) 227, 1287
ZraI GACGTC 1 cut(s) 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.