MD15G1288500.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012508
Physical Location & Seq
Reverse (-)
26574596 .. 26577585
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1288500.v1.1.491

Sequence Viewer

Length: 2361 bp
ATGAGCTCCCAAAGCCTCCATTTTTTACCATGCTTTCTTCTTCTTCTTCTTCTTCTCTCGGTCCAATCTTCTGCTCTCGACACAGATGGACTTATCTTACTTTCTTTCAAGTTTTCTATCCTCCGAGACCCTCAGTCCGTTCTTGCAACCTGGAATTCGTACGACGAGACGCCGTGTTCATGGAGAGGCGTCGTTTGCGCGGCGCCGCCAACACCATCGTCGGAATACTACCCAAGAGTCGTAGGTTTGAATCTTCCCAACTCTCAGCTTCTGGGTTCCATCCCTGCCAACCTTGGCATGATCGAACACCTTCAAAGTCTCGATCTTTCCAACAATTCCCTCAACGGGTCCCTCCCACAGTCGCTCTTCAACGCCACGGAGCTTCGGGTTCTTGATTTGTCGTACAATTTGATCTCCGGCGAGCTGCCGGACGCGGTGTCGCAGCTCACCAACCTCCAGAGTCTCAACCTCTCCGACAACGCCTTGGCGGGAAAATTGCCCACCAATCTGTCTTCTCTGCCCTCTCTAACGATTATTTCTTTGAAGAATAATCATTTCTCCGGCGGCCTTCCCAGCGGGTTGGAATCCGTTCAGGTTTTGGATCTGTCTTCGAATCTGATCAACGGCTCCCTGCCGTCTGATTTCGGCGGCAACAGTCTGAGTTACTTAAACGTCTCCCACAACGGAATTTCCGGGAAAATACCACCGCAATTTTCCGAGAAAATTCCAGGTAAAGCCAAAACCGACCTATCTTTCAACAATCTCACCGGCGAACTCCCGGAATCTCAGGTGTTCTTGAACCAAGAAACTGGGTCTTTTACCGGCAATCCTGATCTGTGTGGCCCACCGACCAAGAACCCATGTCCGATTCCTTCTTCTCCTTCCTCTTCACCCAATGCTTCGTCTCCGAATTCTCCTCCGGCAATTGCTGCAATTCCCAGGAACCCAGAAGCCGTTTCGCCGATGTCTGCAAATGAGCATTCGCAGCAGAGCCAGACCGGGCTCAGACCGGCCGCCATTGTCGGAATAGTAGCCGGCGACATAGCCGGAATCGGAATCCTCGCGCTGATTTTCCTGTACATTTACAGATTGAAGAAGAAGAAGAAGAAGAGCACCAAGGCCGAGATCACAGCAACGCTGAAGAAAGACGCTAATACGAATTCAACGCCGGTAGTCAACGACTGGTCGTCTTCCTCGTCGGAATCGAAAGGGTTCACCAGATGGTCGTGCTTGAGAAAGCGAGGCGAAGACGAAGACAGCTCCAACACGACAGCCTCCGAATCCGAAGAACATCAGACGGAGCAGAATCCCCAAAGGGGTCACGAAATCAAACGGCAGCAAGAGCAGGAAAAGAGCAAAGGCGGGACCTTGGTCACCGTCGATGGCGAAAAGGAGCTAGAACTGGAGACACTGCTCAAAGCCTCGGCGTACATTCTGGGCGCCACCGGTTCGAGCATAATGTACAAGGCAGTGCTCGAAGACGGAAGCTCGTTGGCAGTTCGGAGGATCGGGGAGCAGAGTGTGGACCGGTTCAAGGATTTCGAGAACCAAATCAAGCTCGTGGCCAAATTGGTTCACCCGAACCTGGTTCGAATTCGTGGATTCTATTGGGGGATCGATGAGAAGCTCATTATCTATGATTTCGTCCCAAATGGCAGCCTCGCAAACGCTCGTTACAGGAAGGTGGGCACATCGCCTTGTCATCTACCGTGGGAAGCGAGGCTCAAGATAGCGAAAGGGGTTGCCCGTGGGCTCGCGTACCTCCACGAGAAGAAGCATGTCCATGGGAACCTCAAGCCTACCAACATTCTCCTAGACGCGGACATGGAGCCCCGGATTGGTGATTTTGGACTCGAGAGACTTTTGTCCGGCGACACAAGTTACAAAATGGGTAGCTCGACAAGAAATTTTGGAAGCAAGAGATCAACGACTTCACGGGATGGCTTTCAGGACTTTGGACTAGGACCTAGCCCGGGTCCAAGTCCGAGCCCTAGCTCCATGGGCACATCGCCTTATCATTGCCCCGAGTCGCTTCGGAGCCTAAAGCCGAACCCAAAGTGGGATGTGTACTCATTTGGGGTGATATTGCTTGAGCTACTAACCGGGAAAGTTGTGGTTGTGGATGAGGCAGGGCAGGGACTTGGGCTTGGAGTGGATGATGGCGGCAAGGCCTTGAGGATGGCAGATATGGCAATTAGAGGTGAATTGGAGGGCAAAGAGGAGGCCTTGTTGTCGTTGTTCAAGTTAGGGTACAATTGTGCGTCGCCTGTCCCACAAAAGAGACCAACAATGAAAGAAGCCCTACAGGTCCTTGGGAAATTCCCATCTTCTCCTTCATCATTGTATTATTATGCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

787

Amino Acids

84.8

Weight (kDa)

6.91

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 9.4e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 103 - 162 3e-09 Leucine rich repeat
LRR_4 PF12799 126 - 160 9.3e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 127 - 186 2.7e-07 Leucine rich repeat
Pkinase PF00069 479 - 637 1.3e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 494 - 633 6.2e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 651 - 769 4e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 202, 1439
AccII CGCG 5 cut(s) 200, 434, 1064, 1757, 1820
AclWI GGATC 3 cut(s) 609, 1514, 1622
AcoI YGGCCR 2 cut(s) 1011, 1563
AcsI RAATTY 8 cut(s) 154, 687, 723, 910, 1159, 1593, 1906, 2318
AcuI CTGAAG 1 cut(s) 1160
AcyI GRCGYC 4 cut(s) 170, 189, 203, 1440
AfaI GTAC 8 cut(s) 161, 404, 1079, 1430, 1463, 1760, 2069, 2252
AfiI CCNNNNNNNGG 8 cut(s) 345, 1316, 1534, 1585, 1819, 1838, 1973, 2059
AgeI ACCGGT 2 cut(s) 1445, 1527
AhdI GACNNNNNGTC 3 cut(s) 133, 436, 1186
AjnI CCWGG 4 cut(s) 149, 727, 938, 1584
Alw21I GWGCWC 3 cut(s) 8, 1115, 1476
Alw26I GTCTC 9 cut(s) 120, 161, 323, 467, 679, 909, 1400, 1852, 2275
AlwI GGATC 3 cut(s) 609, 1514, 1622
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 3 cut(s) 1853, 1972, 2024
AoxI GGCC 7 cut(s) 565, 841, 1011, 1119, 1563, 2169, 2223
ApeKI GCWGC 6 cut(s) 424, 442, 929, 985, 1336, 1656
ApoI RAATTY 8 cut(s) 154, 687, 723, 910, 1159, 1593, 1906, 2318
AsiGI ACCGGT 2 cut(s) 1445, 1527
Asp700I GAANNNNTTC 3 cut(s) 309, 588, 1211
AspLEI GCGC 4 cut(s) 200, 205, 1066, 1442
AspS9I GGNCC 8 cut(s) 61, 348, 842, 1365, 1525, 1964, 1976, 2308
AsuC2I CCSGG 7 cut(s) 694, 779, 1000, 1834, 1973, 1974, 2104
AsuHPI GGTGA 9 cut(s) 439, 757, 882, 1207, 1366, 1568, 1853, 2092, 2213
AsuII TTCGAA 2 cut(s) 611, 1591
AvaI CYCGRG 3 cut(s) 1853, 1972, 2024
AvaII GGWCC 7 cut(s) 61, 348, 1365, 1525, 1964, 1976, 2308
BaeGI GKGCMC 2 cut(s) 1691, 2006
BaeI ACNNNNGTAYC 2 cut(s) 2242, 2275
BalI TGGCCA 1 cut(s) 1565
BanI GGYRCC 2 cut(s) 202, 1439
BanII GRGCYC 5 cut(s) 8, 1005, 1755, 1833, 1991
BauI CACGAG 2 cut(s) 1559, 1766
BbsI GAAGAC 6 cut(s) 504, 600, 1182, 1254, 1260, 1485
Bbv12I GWGCWC 3 cut(s) 8, 1115, 1476
BbvI GCAGC 6 cut(s) 411, 454, 916, 997, 1348, 1668
BccI CCATC 9 cut(s) 80, 223, 287, 1215, 1376, 1934, 2153, 2173, 2332
BceAI ACGGC 5 cut(s) 157, 619, 640, 938, 1349
BciT130I CCWGG 4 cut(s) 151, 729, 940, 1586
BclI TGATCA 1 cut(s) 618
BcnI CCSGG 7 cut(s) 694, 779, 1000, 1834, 1973, 1974, 2104
BcoDI GTCTC 9 cut(s) 120, 161, 323, 467, 679, 909, 1400, 1852, 2275
BfaI CTAG 5 cut(s) 1397, 1814, 1961, 1968, 1992
BfmI CTRYAG 1 cut(s) 2303
BfoI RGCGCY 2 cut(s) 206, 1443
Bme18I GGWCC 7 cut(s) 61, 348, 1365, 1525, 1964, 1976, 2308
BmeRI GACNNNNNGTC 3 cut(s) 133, 436, 1186
BmeT110I CYCGRG 3 cut(s) 1853, 1972, 2024
BmgT120I GGNCC 8 cut(s) 61, 348, 842, 1365, 1525, 1964, 1976, 2308
BmrI ACTGGG 1 cut(s) 819
BmuI ACTGGG 1 cut(s) 819
BoxI GACNNNNGTC 2 cut(s) 1370, 1864
BpiI GAAGAC 6 cut(s) 504, 600, 1182, 1254, 1260, 1485
BpmI CTGGAG 2 cut(s) 440, 1424
Bpu14I TTCGAA 2 cut(s) 611, 1591
BpuEI CTTGAG 5 cut(s) 1252, 1709, 1778, 2111, 2194
BpuMI CCSGG 7 cut(s) 694, 779, 1000, 1834, 1973, 1974, 2104
Bsa29I ATCGAT 1 cut(s) 1617
BsaHI GRCGYC 4 cut(s) 170, 189, 203, 1440
BsaI GGTCTC 2 cut(s) 120, 2275
BsaWI WCCGGW 2 cut(s) 1445, 1527
BsaXI ACNNNNNCTCC 2 cut(s) 2213, 2243
Bsc4I CCNNNNNNNGG 8 cut(s) 345, 1316, 1534, 1585, 1819, 1838, 1973, 2059
Bse118I RCCGGY 7 cut(s) 767, 821, 1009, 1034, 1168, 1445, 1527
Bse1I ACTGG 3 cut(s) 814, 1187, 1407
Bse3DI GCAATG 1 cut(s) 2017
BseBI CCWGG 4 cut(s) 151, 729, 940, 1586
BseCI ATCGAT 1 cut(s) 1617
BseGI GGATG 6 cut(s) 279, 1945, 2068, 2128, 2161, 2184
BseLI CCNNNNNNNGG 8 cut(s) 345, 1316, 1534, 1585, 1819, 1838, 1973, 2059
BseMI GCAATG 1 cut(s) 2017
BseMII CTCAG 5 cut(s) 146, 278, 650, 800, 1018
BseNI ACTGG 3 cut(s) 814, 1187, 1407
BseRI GAGGAG 2 cut(s) 906, 2234
BseSI GKGCMC 2 cut(s) 1691, 2006
BseX3I CGGCCG 1 cut(s) 1011
BseXI GCAGC 6 cut(s) 411, 454, 916, 997, 1348, 1668
BseYI CCCAGC 1 cut(s) 572
Bsh1236I CGCG 5 cut(s) 200, 434, 1064, 1757, 1820
Bsh1285I CGRYCG 1 cut(s) 1014
BshFI GGCC 7 cut(s) 567, 843, 1013, 1121, 1565, 2171, 2225
BshNI GGYRCC 2 cut(s) 202, 1439
BshTI ACCGGT 2 cut(s) 1445, 1527
BshVI ATCGAT 1 cut(s) 1617
BsiEI CGRYCG 1 cut(s) 1014
BsiHKAI GWGCWC 3 cut(s) 8, 1115, 1476
BsiHKCI CYCGRG 3 cut(s) 1853, 1972, 2024
BsiWI CGTACG 1 cut(s) 159
BslFI GGGAC 5 cut(s) 334, 1378, 1631, 2151, 2255
BslI CCNNNNNNNGG 8 cut(s) 345, 1316, 1534, 1585, 1819, 1838, 1973, 2059
BsmAI GTCTC 9 cut(s) 120, 161, 323, 467, 679, 909, 1400, 1852, 2275
BsmBI CGTCTC 3 cut(s) 161, 679, 909
BsmFI GGGAC 5 cut(s) 334, 1378, 1631, 2151, 2255
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 7 cut(s) 567, 843, 1013, 1121, 1565, 2171, 2225
Bso31I GGTCTC 2 cut(s) 120, 2275
BsoBI CYCGRG 3 cut(s) 1853, 1972, 2024
Bsp119I TTCGAA 2 cut(s) 611, 1591
Bsp1286I GDGCHC 9 cut(s) 8, 1005, 1115, 1476, 1691, 1755, 1833, 1991, 2006
Bsp1407I TGTACA 2 cut(s) 1077, 1461
Bsp19I CCATGG 2 cut(s) 1783, 1998
BspANI GGCC 7 cut(s) 567, 843, 1013, 1121, 1565, 2171, 2225
BspCNI CTCAG 5 cut(s) 145, 277, 651, 799, 1017
BspDI ATCGAT 1 cut(s) 1617
BspFNI CGCG 5 cut(s) 200, 434, 1064, 1757, 1820
BspPI GGATC 3 cut(s) 609, 1514, 1622
BspQI GCTCTTC 2 cut(s) 371, 1103
BspT104I TTCGAA 2 cut(s) 611, 1591
BspT107I GGYRCC 2 cut(s) 202, 1439
BspTNI GGTCTC 2 cut(s) 120, 2275
BsrDI GCAATG 1 cut(s) 2017
BsrFI RCCGGY 7 cut(s) 767, 821, 1009, 1034, 1168, 1445, 1527
BsrGI TGTACA 2 cut(s) 1077, 1461
BsrI ACTGG 3 cut(s) 814, 1187, 1407
BssAI RCCGGY 7 cut(s) 767, 821, 1009, 1034, 1168, 1445, 1527
BssNI GRCGYC 4 cut(s) 170, 189, 203, 1440
BssSI CACGAG 2 cut(s) 1559, 1766
BssT1I CCWWGG 7 cut(s) 292, 483, 1116, 1368, 1783, 1998, 2311
Bst2BI CACGAG 2 cut(s) 1559, 1766
Bst2UI CCWGG 4 cut(s) 151, 729, 940, 1586
Bst4CI ACNGT 4 cut(s) 360, 656, 1378, 1710
Bst6I CTCTTC 3 cut(s) 371, 892, 1103
BstACI GRCGYC 4 cut(s) 170, 189, 203, 1440
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 2 cut(s) 1077, 1461
BstBI TTCGAA 2 cut(s) 611, 1591
BstC8I GCNNGC 3 cut(s) 422, 1036, 1755
BstDEI CTNAG 5 cut(s) 132, 264, 659, 786, 1004
BstDSI CCRYGG 5 cut(s) 375, 1709, 1747, 1783, 1998
BstEII GGTNACC 1 cut(s) 1372
BstF5I GGATG 6 cut(s) 279, 1945, 2068, 2128, 2161, 2184
BstFNI CGCG 5 cut(s) 200, 434, 1064, 1757, 1820
BstH2I RGCGCY 2 cut(s) 206, 1443
BstHHI GCGC 4 cut(s) 200, 205, 1066, 1442
BstMAI GTCTC 9 cut(s) 120, 161, 323, 467, 679, 909, 1400, 1852, 2275
BstMCI CGRYCG 1 cut(s) 1014
BstMWI GCNNNNNNNGC 8 cut(s) 12, 195, 573, 929, 985, 1342, 2001, 2189
BstNI CCWGG 4 cut(s) 151, 729, 940, 1586
BstNSI RCATGY 1 cut(s) 1781
BstPAI GACNNNNGTC 2 cut(s) 1370, 1864
BstPI GGTNACC 1 cut(s) 1372
BstSFI CTRYAG 1 cut(s) 2303
BstSLI GKGCMC 2 cut(s) 1691, 2006
BstUI CGCG 5 cut(s) 200, 434, 1064, 1757, 1820
BstV1I GCAGC 6 cut(s) 411, 454, 916, 997, 1348, 1668
BstV2I GAAGAC 6 cut(s) 504, 600, 1182, 1254, 1260, 1485
BstX2I RGATCY 1 cut(s) 601
BstXI CCANNNNNNTGG 2 cut(s) 580, 809
BstYI RGATCY 1 cut(s) 601
BstZI CGGCCG 1 cut(s) 1011
Bsu15I ATCGAT 1 cut(s) 1617
BsuRI GGCC 7 cut(s) 567, 843, 1013, 1121, 1565, 2171, 2225
BsuTUI ATCGAT 1 cut(s) 1617
BtgI CCRYGG 5 cut(s) 375, 1709, 1747, 1783, 1998
BtgZI GCGATG 2 cut(s) 1677, 1992
BtsCI GGATG 6 cut(s) 279, 1945, 2068, 2128, 2161, 2184
BtsI GCAGTG 2 cut(s) 1409, 1476
BtsIMutI CAGTG 2 cut(s) 1409, 1476
Cac8I GCNNGC 3 cut(s) 422, 1036, 1755
CaiI CAGNNNCTG 1 cut(s) 271
CfoI GCGC 4 cut(s) 200, 205, 1066, 1442
Cfr10I RCCGGY 7 cut(s) 767, 821, 1009, 1034, 1168, 1445, 1527
Cfr13I GGNCC 8 cut(s) 61, 348, 842, 1365, 1525, 1964, 1976, 2308
Cfr9I CCCGGG 1 cut(s) 1972
ClaI ATCGAT 1 cut(s) 1617
CseI GACGC 6 cut(s) 178, 178, 440, 1157, 1826, 2250
CsiI ACCWGGT 1 cut(s) 1584
Csp6I GTAC 8 cut(s) 160, 403, 1078, 1429, 1462, 1759, 2068, 2251
CspAI ACCGGT 2 cut(s) 1445, 1527
CviAII CATG 8 cut(s) 30, 180, 298, 861, 1778, 1784, 1825, 1999
CviQI GTAC 8 cut(s) 160, 403, 1078, 1429, 1462, 1759, 2068, 2251
DdeI CTNAG 5 cut(s) 132, 264, 659, 786, 1004
DinI GGCGCC 2 cut(s) 204, 1441
DriI GACNNNNNGTC 3 cut(s) 133, 436, 1186
EaeI YGGCCR 2 cut(s) 1011, 1563
EagI CGGCCG 1 cut(s) 1011
Eam1104I CTCTTC 3 cut(s) 371, 892, 1103
Eam1105I GACNNNNNGTC 3 cut(s) 133, 436, 1186
EarI CTCTTC 3 cut(s) 371, 892, 1103
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 1 cut(s) 1011
Eco130I CCWWGG 7 cut(s) 292, 483, 1116, 1368, 1783, 1998, 2311
Eco147I AGGCCT 2 cut(s) 2171, 2225
Eco24I GRGCYC 5 cut(s) 8, 1005, 1755, 1833, 1991
Eco31I GGTCTC 2 cut(s) 120, 2275
Eco47I GGWCC 7 cut(s) 61, 348, 1365, 1525, 1964, 1976, 2308
Eco52I CGGCCG 1 cut(s) 1011
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 1160
Eco88I CYCGRG 3 cut(s) 1853, 1972, 2024
Eco91I GGTNACC 1 cut(s) 1372
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 4 cut(s) 348, 1365, 1964, 2308
EcoO65I GGTNACC 1 cut(s) 1372
EcoRI GAATTC 4 cut(s) 154, 910, 1159, 1593
EcoRII CCWGG 4 cut(s) 149, 727, 938, 1584
EcoT14I CCWWGG 7 cut(s) 292, 483, 1116, 1368, 1783, 1998, 2311
EcoT38I GRGCYC 5 cut(s) 8, 1005, 1755, 1833, 1991
EgeI GGCGCC 2 cut(s) 204, 1441
EheI GGCGCC 2 cut(s) 204, 1441
ErhI CCWWGG 7 cut(s) 292, 483, 1116, 1368, 1783, 1998, 2311
Esp3I CGTCTC 3 cut(s) 161, 679, 909
FaeI CATG 8 cut(s) 33, 183, 301, 864, 1781, 1787, 1828, 2002
FaqI GGGAC 5 cut(s) 334, 1378, 1631, 2151, 2255
FatI CATG 8 cut(s) 29, 179, 297, 860, 1777, 1783, 1824, 1998
FauI CCCGC 3 cut(s) 481, 569, 1355
FbaI TGATCA 1 cut(s) 618
FokI GGATG 6 cut(s) 266, 1952, 2075, 2135, 2168, 2191
FriOI GRGCYC 5 cut(s) 8, 1005, 1755, 1833, 1991
FspBI CTAG 5 cut(s) 1397, 1814, 1961, 1968, 1992
GlaI GCGC 4 cut(s) 199, 204, 1065, 1441
GsaI CCCAGC 1 cut(s) 576
GsuI CTGGAG 2 cut(s) 440, 1424
HaeII RGCGCY 2 cut(s) 206, 1443
HaeIII GGCC 7 cut(s) 567, 843, 1013, 1121, 1565, 2171, 2225
HgaI GACGC 6 cut(s) 178, 178, 440, 1157, 1826, 2250
HhaI GCGC 4 cut(s) 200, 205, 1066, 1442
Hin1I GRCGYC 4 cut(s) 170, 189, 203, 1440
Hin1II CATG 8 cut(s) 33, 183, 301, 864, 1781, 1787, 1828, 2002
Hin6I GCGC 4 cut(s) 198, 203, 1064, 1440
HinP1I GCGC 4 cut(s) 198, 203, 1064, 1440
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HphI GGTGA 9 cut(s) 439, 757, 882, 1207, 1366, 1568, 1853, 2092, 2213
Hpy166II GTNNAC 5 cut(s) 1177, 1215, 1525, 1576, 2068
Hpy8I GTNNAC 5 cut(s) 1177, 1215, 1525, 1576, 2068
Hpy99I CGWCG 6 cut(s) 167, 194, 223, 1201, 1382, 2266
HpyAV CCTTC 6 cut(s) 320, 578, 882, 891, 1675, 2343
HpyCH4III ACNGT 4 cut(s) 360, 656, 1378, 1710
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 3 cut(s) 146, 932, 971
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 195, 573, 929, 985, 1342, 2001, 2189
HpyF3I CTNAG 5 cut(s) 132, 264, 659, 786, 1004
HpySE526I ACGT 1 cut(s) 672
Hsp92I GRCGYC 4 cut(s) 170, 189, 203, 1440
Hsp92II CATG 8 cut(s) 33, 183, 301, 864, 1781, 1787, 1828, 2002
HspAI GCGC 4 cut(s) 198, 203, 1064, 1440
KasI GGCGCC 2 cut(s) 202, 1439
KflI GGGWCCC 1 cut(s) 348
KroI GCCGGC 1 cut(s) 1034
KroNI GCCGGC 1 cut(s) 1036
Ksp22I TGATCA 1 cut(s) 618
LguI GCTCTTC 2 cut(s) 371, 1103
Lsp1109I GCAGC 6 cut(s) 411, 454, 916, 997, 1348, 1668
MabI ACCWGGT 1 cut(s) 1584
MaeI CTAG 5 cut(s) 1397, 1814, 1961, 1968, 1992
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 5 cut(s) 662, 1319, 1372, 1673, 1880
MfeI CAATTG 2 cut(s) 924, 2254
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 9 cut(s) 8, 1005, 1115, 1476, 1691, 1755, 1833, 1991, 2006
MlsI TGGCCA 1 cut(s) 1565
MluNI TGGCCA 1 cut(s) 1565
Mly113I GGCGCC 2 cut(s) 203, 1440
MlyI GAGTC 4 cut(s) 246, 469, 1845, 2036
MmeI TCCRAC 7 cut(s) 201, 354, 498, 561, 1003, 1179, 1287
Mox20I TGGCCA 1 cut(s) 1565
MroNI GCCGGC 1 cut(s) 1034
MroXI GAANNNNTTC 3 cut(s) 309, 588, 1211
MscI TGGCCA 1 cut(s) 1565
MseI TTAA 2 cut(s) 668, 2359
MslI CAYNNNNRTG 1 cut(s) 1782
Msp20I TGGCCA 1 cut(s) 1565
MspA1I CMGCKG 1 cut(s) 576
MteI GCGCNGCGC 1 cut(s) 201
MunI CAATTG 2 cut(s) 924, 2254
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 4 cut(s) 151, 729, 940, 1586
MvnI CGCG 5 cut(s) 200, 434, 1064, 1757, 1820
MwoI GCNNNNNNNGC 8 cut(s) 12, 195, 573, 929, 985, 1342, 2001, 2189
NaeI GCCGGC 1 cut(s) 1036
NarI GGCGCC 2 cut(s) 203, 1440
NciI CCSGG 7 cut(s) 694, 779, 1000, 1834, 1973, 1974, 2104
NcoI CCATGG 2 cut(s) 1783, 1998
NgoMIV GCCGGC 1 cut(s) 1034
NlaIII CATG 8 cut(s) 33, 183, 301, 864, 1781, 1787, 1828, 2002
NmeAIII GCCGAG 2 cut(s) 1147, 1403
NmuCI GTSAC 2 cut(s) 1319, 1372
NspI RCATGY 1 cut(s) 1781
NspV TTCGAA 2 cut(s) 611, 1591
PaeR7I CTCGAG 1 cut(s) 1853
PceI AGGCCT 2 cut(s) 2171, 2225
PciSI GCTCTTC 2 cut(s) 371, 1103
PcsI WCGNNNNNNNCGW 2 cut(s) 1193, 1202
PctI GAATGC 1 cut(s) 979
PdiI GCCGGC 1 cut(s) 1036
PdmI GAANNNNTTC 3 cut(s) 309, 588, 1211
Pfl23II CGTACG 1 cut(s) 159
PfoI TCCNGGA 2 cut(s) 692, 777
PinAI ACCGGT 2 cut(s) 1445, 1527
PleI GAGTC 4 cut(s) 245, 468, 1845, 2035
PluTI GGCGCC 2 cut(s) 206, 1443
PpsI GAGTC 4 cut(s) 245, 468, 1845, 2035
PpuMI RGGWCCY 4 cut(s) 348, 1365, 1964, 2308
PshAI GACNNNNGTC 2 cut(s) 1370, 1864
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 4 cut(s) 348, 1365, 1964, 2308
Psp6I CCWGG 4 cut(s) 149, 727, 938, 1584
PspEI GGTNACC 1 cut(s) 1372
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 4 cut(s) 149, 727, 938, 1584
PspLI CGTACG 1 cut(s) 159
PspPI GGNCC 8 cut(s) 61, 348, 842, 1365, 1525, 1964, 1976, 2308
PspPPI RGGWCCY 4 cut(s) 348, 1365, 1964, 2308
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 601
RsaI GTAC 8 cut(s) 161, 404, 1079, 1430, 1463, 1760, 2069, 2252
RsaNI GTAC 8 cut(s) 160, 403, 1078, 1429, 1462, 1759, 2068, 2251
RseI CAYNNNNRTG 1 cut(s) 1782
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 2 cut(s) 371, 1103
SaqAI TTAA 2 cut(s) 668, 2359
Sau96I GGNCC 8 cut(s) 61, 348, 842, 1365, 1525, 1964, 1976, 2308
SchI GAGTC 4 cut(s) 246, 469, 1845, 2036
SduI GDGCHC 9 cut(s) 8, 1005, 1115, 1476, 1691, 1755, 1833, 1991, 2006
SexAI ACCWGGT 1 cut(s) 1584
SfcI CTRYAG 1 cut(s) 2303
SfoI GGCGCC 2 cut(s) 204, 1441
Sfr274I CTCGAG 1 cut(s) 1853
SfuI TTCGAA 2 cut(s) 611, 1591
SgrAI CRCCGGYG 1 cut(s) 767
SinI GGWCC 7 cut(s) 61, 348, 1365, 1525, 1964, 1976, 2308
SlaI CTCGAG 1 cut(s) 1853
SmaI CCCGGG 1 cut(s) 1974
SmiMI CAYNNNNRTG 1 cut(s) 1782
SmlI CTYRAG 6 cut(s) 1231, 1724, 1793, 1853, 2090, 2173
SmoI CTYRAG 6 cut(s) 1231, 1724, 1793, 1853, 2090, 2173
SseBI AGGCCT 2 cut(s) 2171, 2225
SspDI GGCGCC 2 cut(s) 202, 1439
SspMI CTAG 5 cut(s) 1397, 1814, 1961, 1968, 1992
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 2 cut(s) 2171, 2225
StyI CCWWGG 7 cut(s) 292, 483, 1116, 1368, 1783, 1998, 2311
TaaI ACNGT 4 cut(s) 360, 656, 1378, 1710
TaiI ACGT 1 cut(s) 675
TaqII GACCGA 1 cut(s) 49
TatI WGTACW 3 cut(s) 1077, 1461, 2067
TauI GCSGC 6 cut(s) 203, 208, 567, 651, 1016, 2166
Tru1I TTAA 2 cut(s) 668, 2359
Tru9I TTAA 2 cut(s) 668, 2359
TscAI CASTG 2 cut(s) 1416, 1476
TseFI GTSAC 2 cut(s) 1319, 1372
TseI GCWGC 6 cut(s) 424, 442, 929, 985, 1336, 1656
Tsp45I GTSAC 2 cut(s) 1319, 1372
TspDTI ATGAA 3 cut(s) 168, 2306, 2325
TspGWI ACGGA 6 cut(s) 127, 392, 577, 699, 1313, 1497
TspMI CCCGGG 1 cut(s) 1972
TspRI CASTG 2 cut(s) 1416, 1476
VpaK11BI GGWCC 7 cut(s) 61, 348, 1365, 1525, 1964, 1976, 2308
XapI RAATTY 8 cut(s) 154, 687, 723, 910, 1159, 1593, 1906, 2318
XceI RCATGY 1 cut(s) 1781
XhoI CTCGAG 1 cut(s) 1853
XmaI CCCGGG 1 cut(s) 1972
XmnI GAANNNNTTC 3 cut(s) 309, 588, 1211
XspI CTAG 5 cut(s) 1397, 1814, 1961, 1968, 1992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.