FvH4_1g20720

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010882
Physical Location & Seq
Forward (+)
12866487 .. 12871223
4737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20720.t1

Sequence Viewer

Length: 1110 bp
ATGGGCGCTGCCGGCTCCAAACTCGAGAAGGCTCTCGGCGACCAGTTCCCCGAAGGCGAGCGCTATTTCGGCCTCGAGAATTTCGGCAACACTTGCTATTGTAACAGCGTTTTGCAGGCGCTTTATTTTTGCGTTCCATTTCGTGAACAGTTGTTAGAATACTATTCCAACAGTAAGAATATTGGGGATGCAGAAGAAAATCTCTTGACGTGCTTAGCCGATTTGTTTACGCAGATAAGTTCACAAAAGAAGAAGACAGGTGTAATTGCTCCAAAGCGTTTCGTACAGAGATTGAAGAAACAAAATGAGCTCTTTCGTAGCTACATGCATCAGGATGCTCATGAATTCTTAAACTTTTTGCTAAATGAACTTGTCGACATACTGGAGAAGGAAGCACAAGCTGCAAAAAGTGATCAAGAAACTTCTTCACCTCCTGAGAAGCTGGCAAATGGTCCGAAGAATGGTCAAGCTAATGGTTCTCATAAAGATCCCTTAGTTACGTGGGTGCACAAAAATTTTCAGGGAATACTTACCAATGAAACAAGGTGCTTACGGTGTGAGACAGTGACTGCAAGAGATGAAACATTTTTTGACTTGAGCCTTGATATTGAACAGAACAGTTCAATAACAAGCTGTTTGAAAAACTTCAGTTCCACAGAGACATTGAATGCTGAGGACAAATTTTTTTGCGACAAGTGCTGCAGCTTGCAAGAAGCGCAGAAGAGGATGAAGATAAAGAAACCACCTCACGTTCTGGTCATACATCTGAAGCGGTTCAAGTACATTGAGCAGCTTGGCCGTTACAAGAAGCTATCTTACCGGGTTGTATTTCCTCTTGAGCTCAAGTTGAGCAACACGGTTGAAGATGCAGAGTCTGAGTATTCCCTGTTTGCAGTAGTTGTCCATGTCGGGAGTGGTCCAAACCATGGACACTATGTTAGCCTTGTGAAAAGCCATAATCACTGGTTATTTTTTGATGACGAAAATGTGGAGATGATTGATGAGTCTGCTGTGCAAACTTTCTTTGGGTCAGCACAAGAGTATTCGAGTAATACAGATCACGGATACATCCTGTTCTATGAAAGCCTCGGCACCAGTAACAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH

Protein Analysis

370

Amino Acids

42.05

Weight (kDa)

5.88

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 23 - 360 6.3e-67 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 24 - 329 1.7e-26 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22310 AT2G22310 AT2G22310 AT4G39910
fragaria_vesca FvH4_1g20720
malus_domestica MD01G1031200.v1.1 MD15G1318500.v1.1
prunus_persica Prupe.6G187800_v2.0.a1
pyrus_communis pycom01g05860 pycom15g28490
rosa_chinensis RchiOBHm_Chr2g0113231
rosa_laevigata RLG00000018049
rosa_multiflora Rmu_sc0006838.1_g000020 Rmu_sc0008490.1_g000004
rosa_roxburghii Rroxscaffold_2G00129520 Rroxscaffold_2G00129770
rosa_rugosa Rorug02G0186000
rosa_samantha Rh2CG244600 Rh2DG248500
rosa_wichuraiana Rw2G018810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 1 cut(s) 926
AccI GTMKAC 1 cut(s) 375
AciI CCGC 1 cut(s) 772
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 4 cut(s) 79, 344, 514, 680
AcuI CTGAAG 2 cut(s) 631, 788
AfaI GTAC 2 cut(s) 285, 782
AfeI AGCGCT 1 cut(s) 62
AfiI CCNNNNNNNGG 2 cut(s) 461, 926
AgsI TTSAA 7 cut(s) 295, 611, 624, 640, 667, 778, 863
AjiI CACGTC 1 cut(s) 210
Alw21I GWGCWC 3 cut(s) 312, 510, 843
Alw26I GTCTC 2 cut(s) 554, 653
Alw44I GTGCAC 1 cut(s) 506
AlwI GGATC 1 cut(s) 482
AlwNI CAGNNNCTG 2 cut(s) 569, 875
Ama87I CYCGRG 2 cut(s) 23, 74
Aor51HI AGCGCT 1 cut(s) 62
AoxI GGCC 2 cut(s) 70, 796
ApaLI GTGCAC 1 cut(s) 506
ApeKI GCWGC 5 cut(s) 8, 401, 699, 702, 790
ApoI RAATTY 4 cut(s) 79, 344, 514, 680
Asp700I GAANNNNTTC 2 cut(s) 644, 773
AspLEI GCGC 4 cut(s) 8, 63, 121, 718
AspS9I GGNCC 2 cut(s) 452, 917
AsuC2I CCSGG 1 cut(s) 821
AsuHPI GGTGA 1 cut(s) 420
AvaI CYCGRG 2 cut(s) 23, 74
AvaII GGWCC 2 cut(s) 452, 917
BaeGI GKGCMC 1 cut(s) 510
BanI GGYRCC 1 cut(s) 1091
BanII GRGCYC 2 cut(s) 312, 843
BarI GAAGNNNNNNTAC 2 cut(s) 800, 832
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 3 cut(s) 312, 510, 843
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 4 cut(s) 388, 686, 714, 802
BceAI ACGGC 1 cut(s) 783
BciVI GTATCC 1 cut(s) 1058
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 821
BcoDI GTCTC 2 cut(s) 554, 653
BfmI CTRYAG 1 cut(s) 700
BfoI RGCGCY 3 cut(s) 9, 64, 122
BfuI GTATCC 1 cut(s) 1058
BisI GCNGC 5 cut(s) 9, 402, 700, 703, 791
BlpI GCTNAGC 1 cut(s) 214
BlsI GCNGC 5 cut(s) 10, 403, 701, 704, 792
Bme1390I CCNGG 1 cut(s) 821
Bme18I GGWCC 2 cut(s) 452, 917
BmeT110I CYCGRG 2 cut(s) 23, 74
BmgBI CACGTC 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 452, 917
BmiI GGNNCC 2 cut(s) 16, 1093
BmrFI CCNGG 1 cut(s) 821
BmsI GCATC 4 cut(s) 178, 325, 337, 856
BpiI GAAGAC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 404
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 1 cut(s) 214
BpuEI CTTGAG 3 cut(s) 616, 827, 857
BpuMI CCSGG 1 cut(s) 821
BsaAI YACGTR 1 cut(s) 501
BsaJI CCNNGG 2 cut(s) 925, 1087
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 926
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 4 cut(s) 43, 387, 968, 1095
BseDI CCNNGG 2 cut(s) 925, 1087
BseGI GGATG 4 cut(s) 193, 340, 732, 1068
BseLI CCNNNNNNNGG 2 cut(s) 461, 926
BseMII CTCAG 3 cut(s) 426, 663, 867
BseNI ACTGG 4 cut(s) 43, 387, 968, 1095
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 4 cut(s) 388, 686, 714, 802
BshFI GGCC 2 cut(s) 72, 798
BshNI GGYRCC 1 cut(s) 1091
BsiHKAI GWGCWC 3 cut(s) 312, 510, 843
BsiHKCI CYCGRG 2 cut(s) 23, 74
BsiSI CCGG 2 cut(s) 12, 820
BslI CCNNNNNNNGG 2 cut(s) 461, 926
BsmAI GTCTC 2 cut(s) 554, 653
BsmI GAATGC 1 cut(s) 673
BsnI GGCC 2 cut(s) 72, 798
BsoBI CYCGRG 2 cut(s) 23, 74
Bsp1286I GDGCHC 3 cut(s) 312, 510, 843
Bsp143I GATC 3 cut(s) 412, 487, 1057
Bsp1720I GCTNAGC 1 cut(s) 214
Bsp19I CCATGG 1 cut(s) 925
BspACI CCGC 1 cut(s) 772
BspANI GGCC 2 cut(s) 72, 798
BspCNI CTCAG 3 cut(s) 427, 664, 868
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 16, 1093
BspMAI CTGCAG 1 cut(s) 704
BspPI GGATC 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 1091
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 4 cut(s) 43, 387, 968, 1095
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 925, 1087
BssMI GATC 3 cut(s) 412, 487, 1057
BssT1I CCWWGG 1 cut(s) 925
Bst4CI ACNGT 6 cut(s) 150, 173, 555, 565, 620, 859
Bst6I CTCTTC 1 cut(s) 716
BstAPI GCANNNNNTGC 2 cut(s) 93, 401
BstBAI YACGTR 1 cut(s) 501
BstC8I GCNNGC 5 cut(s) 13, 59, 117, 444, 707
BstDEI CTNAG 5 cut(s) 214, 435, 493, 672, 876
BstDSI CCRYGG 1 cut(s) 925
BstF5I GGATG 4 cut(s) 193, 340, 732, 1068
BstH2I RGCGCY 3 cut(s) 9, 64, 122
BstHHI GCGC 4 cut(s) 8, 63, 121, 718
BstKTI GATC 3 cut(s) 415, 490, 1060
BstMAI GTCTC 2 cut(s) 554, 653
BstMBI GATC 3 cut(s) 412, 487, 1057
BstMWI GCNNNNNNNGC 6 cut(s) 12, 69, 93, 401, 696, 715
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 819
BstSFI CTRYAG 1 cut(s) 700
BstSLI GKGCMC 1 cut(s) 510
BstV1I GCAGC 4 cut(s) 388, 686, 714, 802
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BsuI GTATCC 1 cut(s) 1058
BsuRI GGCC 2 cut(s) 72, 798
BtgI CCRYGG 1 cut(s) 925
BtrI CACGTC 1 cut(s) 210
BtsCI GGATG 4 cut(s) 193, 340, 732, 1068
BtsIMutI CAGTG 2 cut(s) 570, 961
Cac8I GCNNGC 5 cut(s) 13, 59, 117, 444, 707
CaiI CAGNNNCTG 2 cut(s) 569, 875
CciI TCATGA 1 cut(s) 340
CfoI GCGC 4 cut(s) 8, 63, 121, 718
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 452, 917
Csp6I GTAC 2 cut(s) 284, 781
CviAII CATG 4 cut(s) 325, 341, 905, 926
CviQI GTAC 2 cut(s) 284, 781
DdeI CTNAG 5 cut(s) 214, 435, 493, 672, 876
DpnI GATC 3 cut(s) 414, 489, 1059
DpnII GATC 3 cut(s) 412, 487, 1057
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
Ecl136II GAGCTC 2 cut(s) 310, 841
Eco130I CCWWGG 1 cut(s) 925
Eco24I GRGCYC 2 cut(s) 312, 843
Eco47I GGWCC 2 cut(s) 452, 917
Eco47III AGCGCT 1 cut(s) 62
Eco53kI GAGCTC 2 cut(s) 310, 841
Eco57I CTGAAG 2 cut(s) 631, 788
Eco88I CYCGRG 2 cut(s) 23, 74
EcoICRI GAGCTC 2 cut(s) 310, 841
EcoRI GAATTC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 925
EcoT22I ATGCAT 1 cut(s) 330
EcoT38I GRGCYC 2 cut(s) 312, 843
ErhI CCWWGG 1 cut(s) 925
FaeI CATG 4 cut(s) 328, 344, 908, 929
FatI CATG 4 cut(s) 324, 340, 904, 925
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 5 cut(s) 9, 402, 700, 703, 791
FokI GGATG 4 cut(s) 200, 347, 739, 1055
FriOI GRGCYC 2 cut(s) 312, 843
Fsp4HI GCNGC 5 cut(s) 9, 402, 700, 703, 791
GlaI GCGC 4 cut(s) 7, 62, 120, 717
GluI GCNGC 5 cut(s) 9, 402, 700, 703, 791
GsuI CTGGAG 1 cut(s) 404
HaeII RGCGCY 3 cut(s) 9, 64, 122
HaeIII GGCC 2 cut(s) 72, 798
HapII CCGG 2 cut(s) 12, 820
HhaI GCGC 4 cut(s) 8, 63, 121, 718
Hin1II CATG 4 cut(s) 328, 344, 908, 929
Hin6I GCGC 4 cut(s) 6, 61, 119, 716
HinP1I GCGC 4 cut(s) 6, 61, 119, 716
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 2 cut(s) 872, 1004
HpaII CCGG 2 cut(s) 12, 820
HphI GGTGA 1 cut(s) 420
Hpy166II GTNNAC 5 cut(s) 146, 228, 242, 376, 508
Hpy188I TCNGA 3 cut(s) 456, 768, 877
Hpy8I GTNNAC 5 cut(s) 146, 228, 242, 376, 508
HpyAV CCTTC 3 cut(s) 22, 47, 382
HpyCH4III ACNGT 6 cut(s) 150, 173, 555, 565, 620, 859
HpyCH4IV ACGT 3 cut(s) 209, 500, 750
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 69, 93, 401, 696, 715
HpyF3I CTNAG 5 cut(s) 214, 435, 493, 672, 876
HpySE526I ACGT 3 cut(s) 209, 500, 750
Hsp92II CATG 4 cut(s) 328, 344, 908, 929
HspAI GCGC 4 cut(s) 6, 61, 119, 716
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 3 cut(s) 412, 487, 1057
LmnI GCTCC 2 cut(s) 20, 274
Lsp1109I GCAGC 4 cut(s) 388, 686, 714, 802
LweI GCATC 4 cut(s) 178, 325, 337, 856
MaeII ACGT 3 cut(s) 209, 500, 750
MaeIII GTNAC 5 cut(s) 101, 496, 565, 800, 1097
MalI GATC 3 cut(s) 414, 489, 1059
MboI GATC 3 cut(s) 412, 487, 1057
MboII GAAGA 9 cut(s) 206, 262, 265, 307, 417, 469, 733, 742, 875
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 3 cut(s) 312, 510, 843
MluCI AATT 5 cut(s) 79, 264, 344, 514, 680
MlyI GAGTC 2 cut(s) 881, 1013
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 7 cut(s) 83, 441, 667, 717, 756, 843, 1097
Mph1103I ATGCAT 1 cut(s) 330
MroNI GCCGGC 1 cut(s) 11
MroXI GAANNNNTTC 2 cut(s) 644, 773
MseI TTAA 2 cut(s) 350, 1108
MslI CAYNNNNRTG 1 cut(s) 333
MspI CCGG 2 cut(s) 12, 820
MspR9I CCNGG 1 cut(s) 821
Mva1269I GAATGC 1 cut(s) 673
MwoI GCNNNNNNNGC 6 cut(s) 12, 69, 93, 401, 696, 715
NaeI GCCGGC 1 cut(s) 13
NciI CCSGG 1 cut(s) 821
NcoI CCATGG 1 cut(s) 925
NdeII GATC 3 cut(s) 412, 487, 1057
NgoMIV GCCGGC 1 cut(s) 11
NlaIII CATG 4 cut(s) 328, 344, 908, 929
NlaIV GGNNCC 2 cut(s) 16, 1093
NmeAIII GCCGAG 2 cut(s) 15, 1068
NmuCI GTSAC 1 cut(s) 565
NsiI ATGCAT 1 cut(s) 330
NspI RCATGY 1 cut(s) 328
PaeR7I CTCGAG 2 cut(s) 23, 74
PagI TCATGA 1 cut(s) 340
PctI GAATGC 1 cut(s) 673
PdiI GCCGGC 1 cut(s) 13
PdmI GAANNNNTTC 2 cut(s) 644, 773
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 5 cut(s) 10, 403, 701, 704, 792
PleI GAGTC 2 cut(s) 880, 1012
PpsI GAGTC 2 cut(s) 880, 1012
Ppu21I YACGTR 1 cut(s) 501
Psp124BI GAGCTC 2 cut(s) 312, 843
PspN4I GGNNCC 2 cut(s) 16, 1093
PspPI GGNCC 2 cut(s) 452, 917
PstI CTGCAG 1 cut(s) 704
PstNI CAGNNNCTG 2 cut(s) 569, 875
PsuI RGATCY 1 cut(s) 487
RsaI GTAC 2 cut(s) 285, 782
RsaNI GTAC 2 cut(s) 284, 781
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 2 cut(s) 312, 843
SalI GTCGAC 1 cut(s) 374
SaqAI TTAA 2 cut(s) 350, 1108
SatI GCNGC 5 cut(s) 9, 402, 700, 703, 791
Sau3AI GATC 3 cut(s) 412, 487, 1057
Sau96I GGNCC 2 cut(s) 452, 917
SchI GAGTC 2 cut(s) 881, 1013
ScrFI CCNGG 1 cut(s) 821
SduI GDGCHC 3 cut(s) 312, 510, 843
SfaNI GCATC 4 cut(s) 178, 325, 337, 856
SfcI CTRYAG 1 cut(s) 700
Sfr274I CTCGAG 2 cut(s) 23, 74
SinI GGWCC 2 cut(s) 452, 917
SlaI CTCGAG 2 cut(s) 23, 74
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 5 cut(s) 23, 74, 595, 836, 842
SmoI CTYRAG 5 cut(s) 23, 74, 595, 836, 842
Sse9I AATT 5 cut(s) 79, 264, 344, 514, 680
SsiI CCGC 1 cut(s) 772
SspI AATATT 1 cut(s) 181
SstI GAGCTC 2 cut(s) 312, 843
StyD4I CCNGG 1 cut(s) 819
StyI CCWWGG 1 cut(s) 925
TaaI ACNGT 6 cut(s) 150, 173, 555, 565, 620, 859
TaiI ACGT 3 cut(s) 212, 503, 753
TaqI TCGA 4 cut(s) 24, 75, 375, 1046
TasI AATT 5 cut(s) 79, 264, 344, 514, 680
TatI WGTACW 1 cut(s) 780
Tru1I TTAA 2 cut(s) 350, 1108
Tru9I TTAA 2 cut(s) 350, 1108
TscAI CASTG 2 cut(s) 570, 968
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 5 cut(s) 8, 401, 699, 702, 790
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 6 cut(s) 357, 381, 552, 594, 743, 1095
TspGWI ACGGA 1 cut(s) 1077
TspRI CASTG 2 cut(s) 570, 968
Van91I CCANNNNNTGG 1 cut(s) 926
VneI GTGCAC 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 452, 917
XapI RAATTY 4 cut(s) 79, 344, 514, 680
XceI RCATGY 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 911
XhoI CTCGAG 2 cut(s) 23, 74
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 2 cut(s) 644, 773
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.