MD01G1031200.v1.1

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010882
Physical Location & Seq
Reverse (-)
10947748 .. 10954742
6995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1031200.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGGGTGCTGCGGGCTCCAAACTCGAGAAGGCTCTCGGCGACCAGTTTCCGGAAGGCGAGCGATATTTCGGACTCGAGAATTTCGGCAATACTTGCTATTGTAACAGCGTTTTGCAGGCACTTTATTTTTGTGTTCCGTTTCGTGAGCAGTTGCTAGAATATTATTCCAGCAACAAAAGTGCTGGGGATGCAGAAGAAAATCTGTTGACATGCTTGGCGGATTTGTTTACACAGATAAGTTCACAAAAGAAGAAAACGGGTGTCATTGCTCCAAAGCGTTTCGTACAAAGATTGAAGAAACAAAATGAGCTTTTCCGTAGCTATATGCACCAGGATGCTCATGAATTCTTAAACTTTTTGTTAAATGAACTTGTGGACATACTGGAGAAAGAAGCCCAAGCTGCAAAAAGTGATCAAGAAACTTCTTCACCTCCTGAAAAAATGGGAAATGGTCCGAAGAATGTTCAGGCCAATGGTACTCATAAAGATCCCTTAGTTACCTGGGTGCACAAAAATTTTCAGGGTATACTCACCAATGAAACAAGGTGCTTACGGTGTGAGACAGTGACTGCAAGAGATGAAACATTTTTTGACTTGAGCCTGGATATTGAACAGAACAGTTCAATAACGAGCTGTTTGAAAAACTTCAGTTCCACAGAGACATTAAATGCCGAGGACAAATTTTTTTGCGACAAGTGCTGCAGCTTGCAAGAAGCGCAGAAGAGGATGAAGATAAAAAAGCCGCCTCACATCCTTGTCATCCATCTTAAACGGTTTAAGTACATCGAGCAGCTAGGCCGTTACAAGAAGCTATCTTACCGGGTTGTATTCCCGCTAGAGCTGAAGCTGAGTAACACAGTTGAGGATGTAGAATCTGAGTACTCTCTATTTGCGGTGGTTGTCCATGTTGGTAGTGGGCCAAACCATGGACACTATGTTAGCCTTGTGAAAAGCCATAACCACTGGCTATTTTTTGATGACGAGAATGTGGAGATGATTGATGAGTCTGCTGTGCAAACTTTCTTTGGGTCGGCACAGGAGTATTCGAGTAATACAGATCATGGATACATCTTGTTCTATGAGAGCCTCGGCACCAGTAACAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH

Protein Analysis

370

Amino Acids

42.08

Weight (kDa)

5.88

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 23 - 360 1.3e-66 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 24 - 329 2.9e-26 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22310 AT2G22310 AT2G22310 AT4G39910
fragaria_vesca FvH4_1g20720
malus_domestica MD01G1031200.v1.1 MD15G1318500.v1.1
prunus_persica Prupe.6G187800_v2.0.a1
pyrus_communis pycom01g05860 pycom15g28490
rosa_chinensis RchiOBHm_Chr2g0113231
rosa_laevigata RLG00000018049
rosa_multiflora Rmu_sc0006838.1_g000020 Rmu_sc0008490.1_g000004
rosa_roxburghii Rroxscaffold_2G00129520 Rroxscaffold_2G00129770
rosa_rugosa Rorug02G0186000
rosa_samantha Rh2CG244600 Rh2DG248500
rosa_wichuraiana Rw2G018810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 1 cut(s) 926
AccI GTMKAC 1 cut(s) 526
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 5 cut(s) 11, 218, 743, 833, 893
AclWI GGATC 1 cut(s) 482
AcsI RAATTY 4 cut(s) 79, 344, 514, 680
AcuI CTGAAG 2 cut(s) 631, 863
AfaI GTAC 4 cut(s) 285, 478, 782, 881
AfiI CCNNNNNNNGG 2 cut(s) 49, 926
AgsI TTSAA 4 cut(s) 295, 611, 624, 640
AjnI CCWGG 3 cut(s) 330, 500, 600
AluBI AGCT 9 cut(s) 310, 321, 401, 633, 705, 793, 811, 841, 847
AluI AGCT 9 cut(s) 310, 321, 401, 633, 705, 793, 811, 841, 847
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 2 cut(s) 554, 653
Alw44I GTGCAC 1 cut(s) 506
AlwI GGATC 1 cut(s) 482
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 2 cut(s) 23, 74
Aor13HI TCCGGA 1 cut(s) 49
AoxI GGCC 3 cut(s) 468, 796, 917
ApaLI GTGCAC 1 cut(s) 506
ApeKI GCWGC 5 cut(s) 8, 401, 699, 702, 790
ApoI RAATTY 4 cut(s) 79, 344, 514, 680
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 1 cut(s) 718
AspS9I GGNCC 2 cut(s) 452, 917
AsuC2I CCSGG 1 cut(s) 821
AsuHPI GGTGA 2 cut(s) 420, 523
AvaI CYCGRG 2 cut(s) 23, 74
AvaII GGWCC 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 510
BanI GGYRCC 1 cut(s) 1091
BanII GRGCYC 1 cut(s) 17
BarI GAAGNNNNNNTAC 2 cut(s) 800, 832
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 4 cut(s) 388, 686, 714, 802
BccI CCATC 1 cut(s) 771
BceAI ACGGC 1 cut(s) 783
BciT130I CCWGG 3 cut(s) 332, 502, 602
BciVI GTATCC 1 cut(s) 1058
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 821
BcoDI GTCTC 2 cut(s) 554, 653
BfaI CTAG 3 cut(s) 155, 794, 836
BfmI CTRYAG 1 cut(s) 700
BfuI GTATCC 1 cut(s) 1058
BisI GCNGC 6 cut(s) 9, 402, 700, 703, 743, 791
BlsI GCNGC 6 cut(s) 10, 403, 701, 704, 744, 792
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 4 cut(s) 332, 502, 602, 821
Bme18I GGWCC 1 cut(s) 452
BmeT110I CYCGRG 2 cut(s) 23, 74
BmgT120I GGNCC 2 cut(s) 452, 917
BmiI GGNNCC 2 cut(s) 16, 1093
BmrFI CCNGG 4 cut(s) 332, 502, 602, 821
BmsI GCATC 2 cut(s) 178, 325
BpmI CTGGAG 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 616
BpuMI CCSGG 1 cut(s) 821
BsaJI CCNNGG 4 cut(s) 501, 672, 925, 1087
BsaWI WCCGGW 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 926
Bse1I ACTGG 4 cut(s) 43, 387, 968, 1095
Bse3DI GCAATG 1 cut(s) 264
BseAI TCCGGA 1 cut(s) 49
BseBI CCWGG 3 cut(s) 332, 502, 602
BseDI CCNNGG 4 cut(s) 501, 672, 925, 1087
BseGI GGATG 6 cut(s) 193, 340, 732, 750, 759, 871
BseLI CCNNNNNNNGG 2 cut(s) 49, 926
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 839, 867
BseNI ACTGG 4 cut(s) 43, 387, 968, 1095
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 4 cut(s) 388, 686, 714, 802
BseYI CCCAGC 1 cut(s) 182
BshFI GGCC 3 cut(s) 470, 798, 919
BshNI GGYRCC 1 cut(s) 1091
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 2 cut(s) 23, 74
BsiSI CCGG 2 cut(s) 50, 820
BslI CCNNNNNNNGG 2 cut(s) 49, 926
BsmAI GTCTC 2 cut(s) 554, 653
BsnI GGCC 3 cut(s) 470, 798, 919
BsoBI CYCGRG 2 cut(s) 23, 74
Bsp1286I GDGCHC 2 cut(s) 17, 510
Bsp13I TCCGGA 1 cut(s) 49
Bsp143I GATC 3 cut(s) 412, 487, 1057
Bsp19I CCATGG 1 cut(s) 925
BspACI CCGC 5 cut(s) 11, 218, 743, 833, 893
BspANI GGCC 3 cut(s) 470, 798, 919
BspCNI CTCAG 2 cut(s) 840, 868
BspEI TCCGGA 1 cut(s) 49
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 16, 1093
BspMAI CTGCAG 1 cut(s) 704
BspPI GGATC 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 1091
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 4 cut(s) 43, 387, 968, 1095
BssECI CCNNGG 4 cut(s) 501, 672, 925, 1087
BssMI GATC 3 cut(s) 412, 487, 1057
BssNAI GTATAC 1 cut(s) 527
BssT1I CCWWGG 1 cut(s) 925
Bst1107I GTATAC 1 cut(s) 527
Bst2UI CCWGG 3 cut(s) 332, 502, 602
Bst4CI ACNGT 5 cut(s) 555, 565, 620, 774, 859
Bst6I CTCTTC 1 cut(s) 716
BstAPI GCANNNNNTGC 1 cut(s) 93
BstC8I GCNNGC 4 cut(s) 13, 59, 117, 707
BstDEI CTNAG 3 cut(s) 493, 848, 876
BstDSI CCRYGG 1 cut(s) 925
BstF5I GGATG 6 cut(s) 193, 340, 732, 750, 759, 871
BstHHI GCGC 1 cut(s) 718
BstKTI GATC 3 cut(s) 415, 490, 1060
BstMAI GTCTC 2 cut(s) 554, 653
BstMBI GATC 3 cut(s) 412, 487, 1057
BstMWI GCNNNNNNNGC 5 cut(s) 93, 188, 401, 696, 715
BstNI CCWGG 3 cut(s) 332, 502, 602
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 4 cut(s) 330, 500, 600, 819
BstSFI CTRYAG 1 cut(s) 700
BstSLI GKGCMC 1 cut(s) 510
BstV1I GCAGC 4 cut(s) 388, 686, 714, 802
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BstZ17I GTATAC 1 cut(s) 527
BsuI GTATCC 1 cut(s) 1058
BsuRI GGCC 3 cut(s) 470, 798, 919
BtgI CCRYGG 1 cut(s) 925
BtsCI GGATG 6 cut(s) 193, 340, 732, 750, 759, 871
BtsIMutI CAGTG 2 cut(s) 570, 961
Cac8I GCNNGC 4 cut(s) 13, 59, 117, 707
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 340
CfoI GCGC 1 cut(s) 718
Cfr13I GGNCC 2 cut(s) 452, 917
Csp6I GTAC 4 cut(s) 284, 477, 781, 880
CviAII CATG 5 cut(s) 210, 341, 905, 926, 1061
CviQI GTAC 4 cut(s) 284, 477, 781, 880
DdeI CTNAG 3 cut(s) 493, 848, 876
DpnI GATC 3 cut(s) 414, 489, 1059
DpnII GATC 3 cut(s) 412, 487, 1057
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 925
Eco24I GRGCYC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 631, 863
Eco88I CYCGRG 2 cut(s) 23, 74
EcoRI GAATTC 1 cut(s) 344
EcoRII CCWGG 3 cut(s) 330, 500, 600
EcoT14I CCWWGG 1 cut(s) 925
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 1 cut(s) 925
FaeI CATG 5 cut(s) 213, 344, 908, 929, 1064
FatI CATG 5 cut(s) 209, 340, 904, 925, 1060
FauI CCCGC 2 cut(s) 4, 840
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 526
Fnu4HI GCNGC 6 cut(s) 9, 402, 700, 703, 743, 791
FokI GGATG 6 cut(s) 200, 347, 737, 739, 746, 878
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 6 cut(s) 9, 402, 700, 703, 743, 791
FspBI CTAG 3 cut(s) 155, 794, 836
GlaI GCGC 1 cut(s) 717
GluI GCNGC 6 cut(s) 9, 402, 700, 703, 743, 791
GsaI CCCAGC 1 cut(s) 186
GsuI CTGGAG 1 cut(s) 404
HaeIII GGCC 3 cut(s) 470, 798, 919
HapII CCGG 2 cut(s) 50, 820
HhaI GCGC 1 cut(s) 718
Hin1II CATG 5 cut(s) 213, 344, 908, 929, 1064
Hin6I GCGC 1 cut(s) 716
HinP1I GCGC 1 cut(s) 716
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HinfI GANTC 3 cut(s) 72, 872, 1004
HpaII CCGG 2 cut(s) 50, 820
HphI GGTGA 2 cut(s) 420, 523
Hpy166II GTNNAC 6 cut(s) 207, 228, 242, 376, 508, 527
Hpy188I TCNGA 3 cut(s) 71, 456, 877
Hpy188III TCNNGA 7 cut(s) 25, 50, 76, 143, 341, 416, 434
Hpy8I GTNNAC 6 cut(s) 207, 228, 242, 376, 508, 527
HpyAV CCTTC 2 cut(s) 22, 47
HpyCH4III ACNGT 5 cut(s) 555, 565, 620, 774, 859
HpyCH4V TGCA 9 cut(s) 115, 191, 328, 404, 508, 572, 702, 709, 1015
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 188, 401, 696, 715
HpyF3I CTNAG 3 cut(s) 493, 848, 876
Hsp92II CATG 5 cut(s) 213, 344, 908, 929, 1064
HspAI GCGC 1 cut(s) 716
Kpn2I TCCGGA 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 3 cut(s) 412, 487, 1057
LmnI GCTCC 2 cut(s) 20, 274
Lsp1109I GCAGC 4 cut(s) 388, 686, 714, 802
LweI GCATC 2 cut(s) 178, 325
MaeI CTAG 3 cut(s) 155, 794, 836
MaeIII GTNAC 6 cut(s) 101, 496, 565, 800, 851, 1097
MalI GATC 3 cut(s) 414, 489, 1059
MboI GATC 3 cut(s) 412, 487, 1057
MboII GAAGA 7 cut(s) 206, 262, 307, 417, 469, 733, 742
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 2 cut(s) 17, 510
MluCI AATT 4 cut(s) 79, 344, 514, 680
MlyI GAGTC 2 cut(s) 66, 1013
MnlI CCTC 6 cut(s) 441, 667, 717, 756, 856, 1097
MroI TCCGGA 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 6 cut(s) 350, 362, 665, 768, 777, 1108
MslI CAYNNNNRTG 1 cut(s) 333
MspI CCGG 2 cut(s) 50, 820
MspR9I CCNGG 4 cut(s) 332, 502, 602, 821
MvaI CCWGG 3 cut(s) 332, 502, 602
MwoI GCNNNNNNNGC 5 cut(s) 93, 188, 401, 696, 715
NciI CCSGG 1 cut(s) 821
NcoI CCATGG 1 cut(s) 925
NdeII GATC 3 cut(s) 412, 487, 1057
NlaIII CATG 5 cut(s) 213, 344, 908, 929, 1064
NlaIV GGNNCC 2 cut(s) 16, 1093
NmeAIII GCCGAG 3 cut(s) 15, 697, 1068
NmuCI GTSAC 1 cut(s) 565
NspI RCATGY 1 cut(s) 213
PaeR7I CTCGAG 2 cut(s) 23, 74
PagI TCATGA 1 cut(s) 340
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 1 cut(s) 872
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 6 cut(s) 10, 403, 701, 704, 744, 792
PleI GAGTC 2 cut(s) 66, 1012
PpsI GAGTC 2 cut(s) 66, 1012
Psp6I CCWGG 3 cut(s) 330, 500, 600
PspFI CCCAGC 1 cut(s) 182
PspGI CCWGG 3 cut(s) 330, 500, 600
PspN4I GGNNCC 2 cut(s) 16, 1093
PspPI GGNCC 2 cut(s) 452, 917
PstI CTGCAG 1 cut(s) 704
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 487
RsaI GTAC 4 cut(s) 285, 478, 782, 881
RsaNI GTAC 4 cut(s) 284, 477, 781, 880
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 6 cut(s) 350, 362, 665, 768, 777, 1108
SatI GCNGC 6 cut(s) 9, 402, 700, 703, 743, 791
Sau3AI GATC 3 cut(s) 412, 487, 1057
Sau96I GGNCC 2 cut(s) 452, 917
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 2 cut(s) 66, 1013
ScrFI CCNGG 4 cut(s) 332, 502, 602, 821
SduI GDGCHC 2 cut(s) 17, 510
SfaNI GCATC 2 cut(s) 178, 325
SfcI CTRYAG 1 cut(s) 700
Sfr274I CTCGAG 2 cut(s) 23, 74
SinI GGWCC 1 cut(s) 452
SlaI CTCGAG 2 cut(s) 23, 74
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 3 cut(s) 23, 74, 595
SmoI CTYRAG 3 cut(s) 23, 74, 595
Sse9I AATT 4 cut(s) 79, 344, 514, 680
SsiI CCGC 5 cut(s) 11, 218, 743, 833, 893
SspI AATATT 1 cut(s) 161
SspMI CTAG 3 cut(s) 155, 794, 836
StyD4I CCNGG 4 cut(s) 330, 500, 600, 819
StyI CCWWGG 1 cut(s) 925
TaaI ACNGT 5 cut(s) 555, 565, 620, 774, 859
TaqI TCGA 4 cut(s) 24, 75, 786, 1046
TasI AATT 4 cut(s) 79, 344, 514, 680
TatI WGTACW 2 cut(s) 780, 879
TauI GCSGC 1 cut(s) 745
TfiI GAWTC 1 cut(s) 872
Tru1I TTAA 6 cut(s) 350, 362, 665, 768, 777, 1108
Tru9I TTAA 6 cut(s) 350, 362, 665, 768, 777, 1108
TscAI CASTG 2 cut(s) 570, 968
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 5 cut(s) 8, 401, 699, 702, 790
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 5 cut(s) 357, 381, 552, 594, 743
TspGWI ACGGA 2 cut(s) 126, 305
TspRI CASTG 2 cut(s) 570, 968
Van91I CCANNNNNTGG 1 cut(s) 926
VneI GTGCAC 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 4 cut(s) 79, 344, 514, 680
XceI RCATGY 1 cut(s) 213
XcmI CCANNNNNNNNNTGG 1 cut(s) 911
XhoI CTCGAG 2 cut(s) 23, 74
XmiI GTMKAC 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 3 cut(s) 155, 794, 836
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.