FvH4_1g21720

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016508
Physical Location & Seq
Reverse (-)
13692863 .. 13693727
865 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21720.t1

Sequence Viewer

Length: 468 bp
ATGATCGGTCGTGAAGTTCTGAGTGAGATTGCTGCACAGACCTCCGATCGCGATCTCTCCGGCCTTCTTCTTCATTCCAACACCGTCGGGGGAGATCCTCCGAAGCAGCAATCGCCTCCCAAGGCAGCAGAGCCTCCAATGCAACCTCCTCGTAATGCTGCAGAGTCTCCCAATCAATCTCCTCCTACAGCAGCAGAGTCTCCCATGCAACATCCTAAGACACCAGAGCCTTCAAAGCTAAAGGGTCGCGCTAAGTTGCGCAAGTGTATTGCTGCTTTCCACAGTGCAAAGGGAACTTGTCTGAAGGAGATCTATGCATCTTATTGGCATCACAGACTCAATATTGGAGAAGATTGCTGCAAGGCTATTAGGGAAGCTGATGATCATTGCGCCGAGACCATTTTCAGTCACTTCAAGAACCCTACTTATGTTCGATTGTTGAAGGAGTATTGCTCAATCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

156

Amino Acids

17.14

Weight (kDa)

8.74

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 88 - 152 3.5e-09 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0016508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03272 AT5G17340
fragaria_vesca FvH4_1g21720
malus_domestica MD01G1086400.v1.1
prunus_persica Prupe.2G194700_v2.0.a1 Prupe.2G194800_v2.0.a1
rosa_multiflora Rmu_ssc0000197.1_g000089
rosa_roxburghii Rroxscaffold_2G00128080 Rroxscaffold_2G00128950
rosa_rugosa Rorug02G0198300 Rorug02G0198600
rosa_samantha Rh2BG266400 Rh2DG263900
rosa_wichuraiana Rw2G020020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 260
AccII CGCG 2 cut(s) 51, 249
AclWI GGATC 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 323
AgsI TTSAA 3 cut(s) 234, 415, 442
AluBI AGCT 2 cut(s) 238, 377
AluI AGCT 2 cut(s) 238, 377
Alw26I GTCTC 3 cut(s) 171, 204, 389
AlwI GGATC 1 cut(s) 89
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 7 cut(s) 32, 106, 125, 158, 191, 272, 357
AspLEI GCGC 3 cut(s) 251, 261, 392
BbvI GCAGC 7 cut(s) 19, 118, 137, 145, 203, 259, 344
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 3 cut(s) 171, 204, 389
BfmI CTRYAG 2 cut(s) 159, 186
BglII AGATCT 1 cut(s) 309
BisI GCNGC 7 cut(s) 33, 107, 126, 159, 192, 273, 358
BlsI GCNGC 7 cut(s) 34, 108, 127, 160, 193, 274, 359
BmsI GCATC 2 cut(s) 326, 337
BplI GAGNNNNNCTC 1 cut(s) 437
BsaBI GATNNNNATC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 385
Bse8I GATNNNNATC 1 cut(s) 51
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 211
BseJI GATNNNNATC 1 cut(s) 51
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 1 cut(s) 11
BseRI GAGGAG 2 cut(s) 138, 171
BseXI GCAGC 7 cut(s) 19, 118, 137, 145, 203, 259, 344
BsgI GTGCAG 1 cut(s) 18
Bsh1236I CGCG 2 cut(s) 51, 249
Bsh1285I CGRYCG 2 cut(s) 10, 49
BshFI GGCC 1 cut(s) 63
BsiEI CGRYCG 2 cut(s) 10, 49
BsiSI CCGG 1 cut(s) 60
BsmAI GTCTC 3 cut(s) 171, 204, 389
BsnI GGCC 1 cut(s) 63
Bso31I GGTCTC 1 cut(s) 389
Bsp143I GATC 6 cut(s) 3, 46, 52, 94, 309, 382
Bsp68I TCGCGA 1 cut(s) 51
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 12
BspFNI CGCG 2 cut(s) 51, 249
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 385
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 6 cut(s) 3, 46, 52, 94, 309, 382
BssT1I CCWWGG 1 cut(s) 120
Bst4CI ACNGT 2 cut(s) 85, 284
BstDEI CTNAG 3 cut(s) 20, 216, 252
BstF5I GGATG 1 cut(s) 211
BstFNI CGCG 2 cut(s) 51, 249
BstHHI GCGC 3 cut(s) 251, 261, 392
BstKTI GATC 6 cut(s) 6, 49, 55, 97, 312, 385
BstMAI GTCTC 3 cut(s) 171, 204, 389
BstMBI GATC 6 cut(s) 3, 46, 52, 94, 309, 382
BstMCI CGRYCG 2 cut(s) 10, 49
BstMWI GCNNNNNNNGC 3 cut(s) 112, 139, 235
BstSFI CTRYAG 2 cut(s) 159, 186
BstUI CGCG 2 cut(s) 51, 249
BstV1I GCAGC 7 cut(s) 19, 118, 137, 145, 203, 259, 344
BstX2I RGATCY 2 cut(s) 94, 309
BstYI RGATCY 2 cut(s) 94, 309
BsuRI GGCC 1 cut(s) 63
BtsCI GGATG 1 cut(s) 211
BtsIMutI CAGTG 1 cut(s) 289
BtuMI TCGCGA 1 cut(s) 51
CfoI GCGC 3 cut(s) 251, 261, 392
CviAII CATG 1 cut(s) 205
CviJI RGCY 6 cut(s) 63, 133, 229, 238, 365, 377
CviKI_1 RGCY 6 cut(s) 63, 133, 229, 238, 365, 377
DdeI CTNAG 3 cut(s) 20, 216, 252
DpnI GATC 6 cut(s) 5, 48, 54, 96, 311, 384
DpnII GATC 6 cut(s) 3, 46, 52, 94, 309, 382
Eco130I CCWWGG 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 1 cut(s) 208
FaiI YATR 3 cut(s) 206, 315, 429
FatI CATG 1 cut(s) 204
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 7 cut(s) 33, 107, 126, 159, 192, 273, 358
FokI GGATG 1 cut(s) 198
Fsp4HI GCNGC 7 cut(s) 33, 107, 126, 159, 192, 273, 358
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 3 cut(s) 250, 260, 391
GluI GCNGC 7 cut(s) 33, 107, 126, 159, 192, 273, 358
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 60
HhaI GCGC 3 cut(s) 251, 261, 392
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 3 cut(s) 249, 259, 390
HinP1I GCGC 3 cut(s) 249, 259, 390
HinfI GANTC 3 cut(s) 164, 197, 336
HpaII CCGG 1 cut(s) 60
Hpy188I TCNGA 4 cut(s) 21, 46, 102, 303
Hpy188III TCNNGA 3 cut(s) 11, 50, 415
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 4 cut(s) 74, 240, 298, 436
HpyCH4III ACNGT 2 cut(s) 85, 284
HpyCH4V TGCA 7 cut(s) 35, 142, 161, 208, 287, 317, 360
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 139, 235
HpyF3I CTNAG 3 cut(s) 20, 216, 252
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 3 cut(s) 249, 259, 390
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 6 cut(s) 3, 46, 52, 94, 309, 382
LpnPI CCDG 2 cut(s) 73, 237
Lsp1109I GCAGC 7 cut(s) 19, 118, 137, 145, 203, 259, 344
LweI GCATC 2 cut(s) 326, 337
MaeIII GTNAC 1 cut(s) 407
MalI GATC 6 cut(s) 5, 48, 54, 96, 311, 384
MboI GATC 6 cut(s) 3, 46, 52, 94, 309, 382
MboII GAAGA 3 cut(s) 59, 62, 362
MflI RGATCY 2 cut(s) 94, 309
MlyI GAGTC 3 cut(s) 173, 206, 330
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 7 cut(s) 52, 108, 126, 144, 156, 159, 192
Mph1103I ATGCAT 1 cut(s) 319
MspI CCGG 1 cut(s) 60
MvnI CGCG 2 cut(s) 51, 249
MwoI GCNNNNNNNGC 3 cut(s) 112, 139, 235
NdeII GATC 6 cut(s) 3, 46, 52, 94, 309, 382
NlaIII CATG 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 1 cut(s) 407
NruI TCGCGA 1 cut(s) 51
NsbI TGCGCA 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 319
PkrI GCNGC 7 cut(s) 34, 108, 127, 160, 193, 274, 359
Ple19I CGATCG 1 cut(s) 49
PleI GAGTC 3 cut(s) 172, 205, 330
PpsI GAGTC 3 cut(s) 172, 205, 330
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 2 cut(s) 94, 309
PvuI CGATCG 1 cut(s) 49
RruI TCGCGA 1 cut(s) 51
SatI GCNGC 7 cut(s) 33, 107, 126, 159, 192, 273, 358
Sau3AI GATC 6 cut(s) 3, 46, 52, 94, 309, 382
SchI GAGTC 3 cut(s) 173, 206, 330
SetI ASST 4 cut(s) 44, 148, 240, 379
SfaNI GCATC 2 cut(s) 326, 337
SfcI CTRYAG 2 cut(s) 159, 186
SspI AATATT 1 cut(s) 343
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 85, 284
TaqI TCGA 1 cut(s) 433
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 7 cut(s) 32, 106, 125, 158, 191, 272, 357
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 1 cut(s) 62
TspRI CASTG 1 cut(s) 289
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.