Rw2G020020

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016508
Physical Location & Seq
Forward (+)
27114892 .. 27115299
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020020.1

Sequence Viewer

Length: 408 bp
ATGACTATATACAGCTCCCCAAAATTTACATCTTTCACAATTCTCATTAGTTTTTTGATCACTTTGCTTACAGAAAACAAAAAAAACTTGAAGAAAGGAGTTGCTGCGCTGCTACTCTTCCTTGCTTGTGCAGCCCTTTGCTTGGCTGCTTCTGATGCTACTGCTGCAGAGCCTCCAAAGCTATCTGGGCACGCTAAGGTGCACACGTGCATTGCTGGTTTCCACACTGCAAGGGGGACTTGTCTGAAGGAGATCTATGCATCTTATTGGAATCACAAACTCAATATTGGAGAAGATTGCTGCAAGGCCATTAAGGAAGCAGATGATGATTGTGCCGAGACCATTTTCAGTCGCTTCAAGAACCCTACTTATGTTCTTTTGTTGAAGGAGCATTGCTCAAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

135

Amino Acids

14.83

Weight (kDa)

8.77

Isoelectric Point (pI)

28.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 70 - 133 3.5e-08 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03272 AT5G17340
fragaria_vesca FvH4_1g21720
malus_domestica MD01G1086400.v1.1
prunus_persica Prupe.2G194700_v2.0.a1 Prupe.2G194800_v2.0.a1
rosa_multiflora Rmu_ssc0000197.1_g000089
rosa_roxburghii Rroxscaffold_2G00128080 Rroxscaffold_2G00128950
rosa_rugosa Rorug02G0198300 Rorug02G0198600
rosa_samantha Rh2BG266400 Rh2DG263900
rosa_wichuraiana Rw2G020020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 23
AcuI CTGAAG 1 cut(s) 266
AcvI CACGTG 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 3 cut(s) 91, 358, 385
AluBI AGCT 2 cut(s) 15, 181
AluI AGCT 2 cut(s) 15, 181
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 332
Alw44I GTGCAC 1 cut(s) 200
AoxI GGCC 1 cut(s) 306
ApaLI GTGCAC 1 cut(s) 200
ApeKI GCWGC 6 cut(s) 104, 109, 131, 146, 164, 300
ApoI RAATTY 1 cut(s) 23
AspLEI GCGC 1 cut(s) 109
BaeGI GKGCMC 2 cut(s) 192, 204
BbrPI CACGTG 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 6 cut(s) 91, 96, 133, 143, 151, 287
BclI TGATCA 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 165
BglII AGATCT 1 cut(s) 252
BisI GCNGC 6 cut(s) 105, 110, 132, 147, 165, 301
BlsI GCNGC 6 cut(s) 106, 111, 133, 148, 166, 302
BmsI GCATC 2 cut(s) 145, 269
BplI GAGNNNNNCTC 1 cut(s) 380
Bpu10I CCTNAGC 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 332
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse3DI GCAATG 2 cut(s) 210, 391
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMI GCAATG 2 cut(s) 210, 391
BseSI GKGCMC 2 cut(s) 192, 204
BseXI GCAGC 6 cut(s) 91, 96, 133, 143, 151, 287
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 204
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 1 cut(s) 308
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 192, 204
Bsp143I GATC 2 cut(s) 57, 252
BspANI GGCC 1 cut(s) 308
BspMAI CTGCAG 1 cut(s) 169
BspTNI GGTCTC 1 cut(s) 332
BsrDI GCAATG 2 cut(s) 210, 391
BssMI GATC 2 cut(s) 57, 252
Bst6I CTCTTC 1 cut(s) 122
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 2 cut(s) 60, 255
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 2 cut(s) 57, 252
BstMWI GCNNNNNNNGC 5 cut(s) 131, 155, 164, 178, 187
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 2 cut(s) 192, 204
BstV1I GCAGC 6 cut(s) 91, 96, 133, 143, 151, 287
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuRI GGCC 1 cut(s) 308
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 192
CfoI GCGC 1 cut(s) 109
CviJI RGCY 6 cut(s) 15, 134, 146, 172, 181, 308
CviKI_1 RGCY 6 cut(s) 15, 134, 146, 172, 181, 308
DdeI CTNAG 1 cut(s) 195
DpnI GATC 2 cut(s) 59, 254
DpnII GATC 2 cut(s) 57, 252
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco31I GGTCTC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 266
Eco72I CACGTG 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 262
FaiI YATR 4 cut(s) 8, 10, 258, 372
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FaqI GGGAC 1 cut(s) 250
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 6 cut(s) 105, 110, 132, 147, 165, 301
Fsp4HI GCNGC 6 cut(s) 105, 110, 132, 147, 165, 301
GlaI GCGC 1 cut(s) 108
GluI GCNGC 6 cut(s) 105, 110, 132, 147, 165, 301
HaeIII GGCC 1 cut(s) 308
HhaI GCGC 1 cut(s) 109
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 1 cut(s) 271
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 2 cut(s) 154, 246
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 241, 379
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 7 cut(s) 131, 167, 202, 210, 230, 260, 303
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 155, 164, 178, 187
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 206
HspAI GCGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 2 cut(s) 57, 252
LmnI GCTCC 2 cut(s) 20, 388
LpnPI CCDG 2 cut(s) 171, 201
Lsp1109I GCAGC 6 cut(s) 91, 96, 133, 143, 151, 287
LweI GCATC 2 cut(s) 145, 269
MaeII ACGT 1 cut(s) 206
MalI GATC 2 cut(s) 59, 254
MboI GATC 2 cut(s) 57, 252
MboII GAAGA 3 cut(s) 103, 109, 305
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 2 cut(s) 192, 204
MluCI AATT 2 cut(s) 23, 39
MnlI CCTC 1 cut(s) 183
Mph1103I ATGCAT 1 cut(s) 262
MseI TTAA 1 cut(s) 312
MwoI GCNNNNNNNGC 5 cut(s) 131, 155, 164, 178, 187
NdeII GATC 2 cut(s) 57, 252
NmeAIII GCCGAG 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 262
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 6 cut(s) 106, 111, 133, 148, 166, 302
PmaCI CACGTG 1 cut(s) 207
PmlI CACGTG 1 cut(s) 207
Ppu21I YACGTR 1 cut(s) 207
PspCI CACGTG 1 cut(s) 207
PstI CTGCAG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 252
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 6 cut(s) 105, 110, 132, 147, 165, 301
Sau3AI GATC 2 cut(s) 57, 252
SduI GDGCHC 2 cut(s) 192, 204
SetI ASST 4 cut(s) 17, 183, 201, 209
SfaNI GCATC 2 cut(s) 145, 269
SfcI CTRYAG 1 cut(s) 165
Sse9I AATT 2 cut(s) 23, 39
SspI AATATT 1 cut(s) 286
TaiI ACGT 1 cut(s) 209
TasI AATT 2 cut(s) 23, 39
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 1 cut(s) 232
TseI GCWGC 6 cut(s) 104, 109, 131, 146, 164, 300
TspRI CASTG 1 cut(s) 232
VneI GTGCAC 1 cut(s) 200
XapI RAATTY 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.