FvH4_1g22940

Violaxanthin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005484
Physical Location & Seq
Forward (+)
14849386 .. 14851846
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22940.t1

Sequence Viewer

Length: 1287 bp
ATGCTTGTATTTACGGATGATATTTGCCAATCTAATGATCAAAATGCAGTGAGTTTCGGGATGGAATTACAACGCGCGGATATTTTGGTGATTGTTGCTGTTACCAATCAAGAATCTGTCGAGTGGATTCAAACTAATAGTGGGAATATTGGAAACATAATCTGTTTCGACTCATCTCCCGAATTGCAAAACAAGCTAGGAGGATCTTATATTCACAGGGAAGGTAAAGGAGATATCTTTGGCAAAATATTTGACACTGACAAATCAAAGAGAAGCAGTGAATCAATGGAAGTGGTACAGACTGTGTCACAGGCGTGGAAGCGGCACAATTCTGATGATATAAGGTTTTGTTTGTTGGTTATAATCAATGGTTATATAAGGCCGGTGCCAAGCCTGCAGAATCTAAGATCAAAAGGTCTTTCTACTCTCAGCTGCATGGTGAAGAACTGTGGACCTCAGATATTAAACTGTCTCTTGGATCCCAACTGTAGGAAAGCTCTTCAATGCTTGAATGAGTGCAGTCCGGTGGATCAAGTGTGTAACTATCGGTGCATTGCTTCGTATGAGAGTCCAGTTCTAGAAGCATTTTCTCTGTGTGTGCTGCAGAAGCACAATTGTCTTGGGTTGGATGCAAAGATCCCTGACAAACCTTATGTGCCACCCATGGTTAACTTTCGAGGGAAGGACTTGTGCCACGAAACTGCTGAAGATCTTTTTGTTGGTTGGTTGGGAAATTTGGATTGGAGTTGGCGTGTCGTGGCAGGGCAGAACCCAGCATATGATCAATTCCCTTGCCAGTACCAACTCTTCTACAGGGGCAAGGCAAAAGGTTCATTTTGGTATGAGCCGGTTTTTCAGGTGAAAACACTGGAGGGGAAGATGGTTTGGAGGAGGCGGAAGTATCGTGTGAAGAGAAACAAAGTCCCAGGGACGTTCCAATTCAGTGTCTTGGACAATGGGGTTGTTTCAAATGAGTCCTGGACAATCGTGGATGTAGCCGATGATCTTAGTTGGGGTTTGTTTTACTACTGTGGTGCTGCAAGAGTTGCCGGACAATCTTATACTGGCGCTGTGCTTGTCAGTCCAGATGGTGCATACCCGAATGATGTGCATAGGAGTAGATTAGTTTCTGCTTTGGAGAACTGTGGTATTAAAGAGTGGGAGCTGTTTACAGTTAACAATTCTTCATGTGCAAATCCTCCGTTGGGGATTCCTGAGGGTTCACGTTTGCATTCCGTGATAGAAGTTAAAGATCAGAAATCTATGTCGGGTTTGGCAGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3479

Protein Analysis

429

Amino Acids

47.96

Weight (kDa)

6.34

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VDE PF07137 144 - 394 7.4e-27 VDE lipocalin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 385
AccII CGCG 2 cut(s) 75, 77
AciI CCGC 3 cut(s) 77, 322, 895
AclWI GGATC 5 cut(s) 211, 473, 486, 537, 631
AcsI RAATTY 1 cut(s) 733
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 2 cut(s) 297, 800
AfiI CCNNNNNNNGG 2 cut(s) 489, 1205
AgsI TTSAA 4 cut(s) 131, 503, 511, 969
AjnI CCWGG 2 cut(s) 925, 977
AjuI GAANNNNNNNTTGG 2 cut(s) 724, 756
AleI CACNNNNGTG 1 cut(s) 313
AluBI AGCT 4 cut(s) 196, 432, 497, 1165
AluI AGCT 4 cut(s) 196, 432, 497, 1165
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 5 cut(s) 211, 473, 486, 537, 631
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 3 cut(s) 432, 601, 1037
ApoI RAATTY 1 cut(s) 733
AspLEI GCGC 2 cut(s) 77, 1070
AspS9I GGNCC 1 cut(s) 452
AsuHPI GGTGA 3 cut(s) 100, 451, 871
AvaII GGWCC 1 cut(s) 452
AxyI CCTNAGG 1 cut(s) 1215
BamHI GGATCC 1 cut(s) 478
BanI GGYRCC 1 cut(s) 385
BarI GAAGNNNNNNTAC 2 cut(s) 791, 823
BbvI GCAGC 3 cut(s) 419, 588, 1024
BccI CCATC 3 cut(s) 55, 874, 1082
BcgI CGANNNNNNTGC 2 cut(s) 1090, 1124
BciT130I CCWGG 2 cut(s) 927, 979
BclI TGATCA 2 cut(s) 37, 781
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 2 cut(s) 197, 578
BfmI CTRYAG 4 cut(s) 395, 487, 602, 811
BfoI RGCGCY 1 cut(s) 1071
BglII AGATCT 1 cut(s) 709
BisI GCNGC 4 cut(s) 323, 433, 602, 1038
BlsI GCNGC 4 cut(s) 324, 434, 603, 1039
Bme1390I CCNGG 2 cut(s) 927, 979
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 2 cut(s) 387, 480
BmrFI CCNGG 2 cut(s) 927, 979
BmsI GCATC 1 cut(s) 619
BpmI CTGGAG 1 cut(s) 890
BsaJI CCNNGG 3 cut(s) 663, 925, 926
BsaWI WCCGGW 1 cut(s) 523
BsaXI ACNNNNNCTCC 2 cut(s) 736, 766
Bsc4I CCNNNNNNNGG 2 cut(s) 489, 1205
Bse118I RCCGGY 2 cut(s) 382, 847
Bse1I ACTGG 4 cut(s) 572, 796, 873, 1069
Bse21I CCTNAGG 1 cut(s) 1215
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 2 cut(s) 927, 979
BseDI CCNNGG 3 cut(s) 663, 925, 926
BseGI GGATG 4 cut(s) 22, 66, 634, 997
BseLI CCNNNNNNNGG 2 cut(s) 489, 1205
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 3 cut(s) 442, 470, 1206
BseNI ACTGG 4 cut(s) 572, 796, 873, 1069
BseRI GAGGAG 1 cut(s) 904
BseXI GCAGC 3 cut(s) 419, 588, 1024
BseYI CCCAGC 1 cut(s) 772
BsgI GTGCAG 1 cut(s) 538
Bsh1236I CGCG 2 cut(s) 75, 77
BshFI GGCC 1 cut(s) 382
BshNI GGYRCC 1 cut(s) 385
BsiSI CCGG 4 cut(s) 383, 524, 848, 1050
BslFI GGGAC 2 cut(s) 908, 943
BslI CCNNNNNNNGG 2 cut(s) 489, 1205
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 908, 943
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 1 cut(s) 382
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 3 cut(s) 77, 322, 895
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 3 cut(s) 441, 469, 1207
BspFNI CGCG 2 cut(s) 75, 77
BspLI GGNNCC 2 cut(s) 387, 480
BspMAI CTGCAG 2 cut(s) 399, 606
BspPI GGATC 5 cut(s) 211, 473, 486, 537, 631
BspQI GCTCTTC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 385
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 2 cut(s) 382, 847
BsrI ACTGG 4 cut(s) 572, 796, 873, 1069
BssAI RCCGGY 2 cut(s) 382, 847
BssECI CCNNGG 3 cut(s) 663, 925, 926
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 927, 979
Bst4CI ACNGT 7 cut(s) 304, 449, 470, 488, 1031, 1145, 1174
Bst6I CTCTTC 3 cut(s) 504, 812, 905
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstC8I GCNNGC 1 cut(s) 395
BstDEI CTNAG 5 cut(s) 404, 428, 456, 1007, 1215
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 4 cut(s) 22, 66, 634, 997
BstFNI CGCG 2 cut(s) 75, 77
BstH2I RGCGCY 1 cut(s) 1071
BstHHI GCGC 2 cut(s) 77, 1070
BstMAI GTCTC 1 cut(s) 476
BstMWI GCNNNNNNNGC 4 cut(s) 193, 394, 607, 1046
BstNI CCWGG 2 cut(s) 927, 979
BstSCI CCNGG 2 cut(s) 925, 977
BstSFI CTRYAG 4 cut(s) 395, 487, 602, 811
BstUI CGCG 2 cut(s) 75, 77
BstV1I GCAGC 3 cut(s) 419, 588, 1024
BstX2I RGATCY 4 cut(s) 203, 478, 636, 709
BstYI RGATCY 4 cut(s) 203, 478, 636, 709
Bsu36I CCTNAGG 1 cut(s) 1215
BsuRI GGCC 1 cut(s) 382
BtgI CCRYGG 1 cut(s) 663
BtsCI GGATG 4 cut(s) 22, 66, 634, 997
BtsI GCAGTG 2 cut(s) 54, 283
BtsIMutI CAGTG 5 cut(s) 54, 255, 283, 866, 949
Cac8I GCNNGC 1 cut(s) 395
CfoI GCGC 2 cut(s) 77, 1070
Cfr10I RCCGGY 2 cut(s) 382, 847
Cfr13I GGNCC 1 cut(s) 452
Csp6I GTAC 2 cut(s) 296, 799
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 3 cut(s) 436, 664, 1188
CviJI RGCY 8 cut(s) 196, 382, 393, 432, 497, 847, 998, 1165
CviKI_1 RGCY 8 cut(s) 196, 382, 393, 432, 497, 847, 998, 1165
CviQI GTAC 2 cut(s) 296, 799
DdeI CTNAG 5 cut(s) 404, 428, 456, 1007, 1215
Eam1104I CTCTTC 3 cut(s) 504, 812, 905
EarI CTCTTC 3 cut(s) 504, 812, 905
EciI GGCGGA 1 cut(s) 910
Eco130I CCWWGG 1 cut(s) 663
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 726
Eco81I CCTNAGG 1 cut(s) 1215
EcoRII CCWGG 2 cut(s) 925, 977
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 3 cut(s) 439, 667, 1191
FaqI GGGAC 2 cut(s) 908, 943
FatI CATG 3 cut(s) 435, 663, 1187
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 2 cut(s) 37, 781
Fnu4HI GCNGC 4 cut(s) 323, 433, 602, 1038
FokI GGATG 4 cut(s) 29, 73, 641, 1004
Fsp4HI GCNGC 4 cut(s) 323, 433, 602, 1038
FspBI CTAG 2 cut(s) 197, 578
GlaI GCGC 2 cut(s) 76, 1069
GluI GCNGC 4 cut(s) 323, 433, 602, 1038
GsaI CCCAGC 1 cut(s) 776
GsuI CTGGAG 1 cut(s) 890
HaeII RGCGCY 1 cut(s) 1071
HaeIII GGCC 1 cut(s) 382
HapII CCGG 4 cut(s) 383, 524, 848, 1050
HhaI GCGC 2 cut(s) 77, 1070
Hin1II CATG 3 cut(s) 439, 667, 1191
Hin6I GCGC 2 cut(s) 75, 1068
HinP1I GCGC 2 cut(s) 75, 1068
HincII GTYRAC 2 cut(s) 670, 1177
HindII GTYRAC 2 cut(s) 670, 1177
HinfI GANTC 8 cut(s) 113, 127, 170, 281, 400, 568, 974, 1210
HpaI GTTAAC 2 cut(s) 670, 1177
HpaII CCGG 4 cut(s) 383, 524, 848, 1050
HphI GGTGA 3 cut(s) 100, 451, 871
Hpy166II GTNNAC 5 cut(s) 452, 670, 1170, 1177, 1223
Hpy188I TCNGA 3 cut(s) 334, 459, 1257
Hpy188III TCNNGA 6 cut(s) 58, 110, 179, 578, 1085, 1214
Hpy8I GTNNAC 5 cut(s) 452, 670, 1170, 1177, 1223
HpyAV CCTTC 2 cut(s) 215, 676
HpyCH4III ACNGT 7 cut(s) 304, 449, 470, 488, 1031, 1145, 1174
HpyCH4IV ACGT 2 cut(s) 932, 1225
HpyF10VI GCNNNNNNNGC 4 cut(s) 193, 394, 607, 1046
HpyF3I CTNAG 5 cut(s) 404, 428, 456, 1007, 1215
HpySE526I ACGT 2 cut(s) 932, 1225
Hsp92II CATG 3 cut(s) 439, 667, 1191
HspAI GCGC 2 cut(s) 75, 1068
Ksp22I TGATCA 2 cut(s) 37, 781
KspAI GTTAAC 2 cut(s) 670, 1177
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 1162
Lsp1109I GCAGC 3 cut(s) 419, 588, 1024
LweI GCATC 1 cut(s) 619
MaeI CTAG 2 cut(s) 197, 578
MaeII ACGT 2 cut(s) 932, 1225
MaeIII GTNAC 3 cut(s) 100, 306, 539
MboII GAAGA 7 cut(s) 454, 491, 719, 799, 889, 922, 1176
MfeI CAATTG 1 cut(s) 613
MflI RGATCY 4 cut(s) 203, 478, 636, 709
MluCI AATT 8 cut(s) 65, 182, 328, 613, 733, 785, 938, 1180
MlyI GAGTC 3 cut(s) 164, 577, 983
MmeI TCCRAC 1 cut(s) 606
MnlI CCTC 9 cut(s) 194, 465, 671, 865, 882, 885, 1209, 1210, 1273
MseI TTAA 5 cut(s) 464, 669, 1152, 1176, 1248
MslI CAYNNNNRTG 1 cut(s) 313
MspA1I CMGCKG 1 cut(s) 432
MspI CCGG 4 cut(s) 383, 524, 848, 1050
MspR9I CCNGG 2 cut(s) 927, 979
MunI CAATTG 1 cut(s) 613
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 2 cut(s) 927, 979
MvnI CGCG 2 cut(s) 75, 77
MwoI GCNNNNNNNGC 4 cut(s) 193, 394, 607, 1046
NcoI CCATGG 1 cut(s) 663
NdeI CATATG 1 cut(s) 778
NlaIII CATG 3 cut(s) 439, 667, 1191
NlaIV GGNNCC 2 cut(s) 387, 480
NmuCI GTSAC 1 cut(s) 306
OliI CACNNNNGTG 1 cut(s) 313
PasI CCCWGGG 1 cut(s) 926
PciSI GCTCTTC 1 cut(s) 504
PctI GAATGC 1 cut(s) 1231
PfeI GAWTC 5 cut(s) 113, 127, 281, 400, 1210
PflFI GACNNNGTC 1 cut(s) 304
PfoI TCCNGGA 1 cut(s) 977
PkrI GCNGC 4 cut(s) 324, 434, 603, 1039
PleI GAGTC 3 cut(s) 164, 576, 982
PpsI GAGTC 3 cut(s) 164, 576, 982
PsiI TTATAA 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 925, 977
PspFI CCCAGC 1 cut(s) 772
PspGI CCWGG 2 cut(s) 925, 977
PspN4I GGNNCC 2 cut(s) 387, 480
PspPI GGNCC 1 cut(s) 452
PstI CTGCAG 2 cut(s) 399, 606
PsuI RGATCY 4 cut(s) 203, 478, 636, 709
PsyI GACNNNGTC 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 432
RsaI GTAC 2 cut(s) 297, 800
RsaNI GTAC 2 cut(s) 296, 799
RseI CAYNNNNRTG 1 cut(s) 313
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 5 cut(s) 464, 669, 1152, 1176, 1248
SatI GCNGC 4 cut(s) 323, 433, 602, 1038
Sau96I GGNCC 1 cut(s) 452
SchI GAGTC 3 cut(s) 164, 577, 983
ScrFI CCNGG 2 cut(s) 927, 979
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 4 cut(s) 395, 487, 602, 811
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 1 cut(s) 313
Sse9I AATT 8 cut(s) 65, 182, 328, 613, 733, 785, 938, 1180
SsiI CCGC 3 cut(s) 77, 322, 895
SspI AATATT 2 cut(s) 148, 249
SspMI CTAG 2 cut(s) 197, 578
StyD4I CCNGG 2 cut(s) 925, 977
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 7 cut(s) 304, 449, 470, 488, 1031, 1145, 1174
TaiI ACGT 2 cut(s) 935, 1228
TaqI TCGA 3 cut(s) 120, 168, 676
TasI AATT 8 cut(s) 65, 182, 328, 613, 733, 785, 938, 1180
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 5 cut(s) 113, 127, 281, 400, 1210
Tru1I TTAA 5 cut(s) 464, 669, 1152, 1176, 1248
Tru9I TTAA 5 cut(s) 464, 669, 1152, 1176, 1248
TscAI CASTG 5 cut(s) 54, 262, 283, 873, 949
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 3 cut(s) 432, 601, 1037
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 2 cut(s) 822, 1176
TspGWI ACGGA 3 cut(s) 29, 1191, 1225
TspRI CASTG 5 cut(s) 54, 262, 283, 873, 949
Tth111I GACNNNGTC 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 1 cut(s) 733
XbaI TCTAGA 1 cut(s) 577
XspI CTAG 2 cut(s) 197, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.