MD10G1028700.v1.1

Violaxanthin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005484
Physical Location & Seq
Forward (+)
3697760 .. 3700515
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1028700.v1.1.491

Sequence Viewer

Length: 1632 bp
ATGACTATGGCTCTGGAAGCAGCTTCAGTTCCACCGCTTTTCAAACCCAGAACCGCAGTTTCATTGAACCGGCGGTTCGCAGCTTCCGCAGCAGCCGGTTTTCTTCATTTTCCATGTTCCCCGTTTGCAGTTGGAAAAACAATTTACCGTGCCCCACCCCCGGTTTTGGCGGTTAAGGAAGTGAATTCGAGCGCGGTGGTGACGGCGGAGGAGACAACACCGGTGAGAATAGTAGCAGTTGTCGGCCAAGGCACCCTCAGCCCTCTCAAGTCCACACCTTGGGAAGAGGTCATGCTCCACACTGCGAAAAGACTGAAATGGGTAGACGAAGCATATGAAATGCTTGTTTTTACCGATGACATCTGCAAATCTGATCATCAAATTGCCGTAAATTTCCACACGGAGTTTCAACGTGCGGATATTTTGGTGATTGTTTCTGTTACGAACCAAGAATCAGTTAAATGGATTCAAACTAATAGTCAGAATATTCGAAACATTATCTGCTTTGACTCATCTCCGAATTTAGCAAACAGATTAGGAGGATATGATATTCGTAGTGAAACCAAGGGAAATATATTAGGCAAAAGCTTTGACACTTCACAAAAATCAATGAACAGTGGATCAGAGGAAGTGATCCAGACTGTATCACAGGCGTGGGATCGGCATAATACGGATGATATAAGGTTTTGTTTATTGGTTATAATTAACACTTACATAAGACCAGTCCCAATCCTGAAGAATCTAAGATCAAGAGGTCTTTCTACCCTGAGCTGCATGGTAAAGAACTGCGGGCCTCAGGTACTAAACTGCCTCTTGGATCCAAATTGTCGGAAAGCACTTCAATGCTTGAATCGGTGCAGCCCAGTAGATCAAGTGTGTAACTATCGGTGCATTGCTTCGTATGAGAGTCCAAATCTAGAAGCATTTTCTCTGTGCGTGTTGCAGAAACATAATTGTCTTGGATTGGATGCAAAGATCCCTGACAAACCGTATGTGCCACCCATGGTTAAATTTCAAGGGAAGGACTTGTGCCACGAAACTGCTGAAGATCTTTTCGTTGGTTGGTTGGGGAGTTTGAATTGGAGTTGGCGTGTTGTGGCAGGGCAGAACCCAGCTTATGATCAATTTCCCTGTCAGTACCAGCTCTTTTATAGGGGGAAGGCCAGAGGGTCATTTTGGTACGAGCCAGTTTTTCAGGTACAAACATTGGAGGGGAAGATGGTCTGGAGGAGGCGGAAGTATCGAGTCAAGAGAAGCAAAATACCGGGGACATTCAACTTTAGTGTCTTGGACAATGGGGTTGTATCGAATGAGTTTTGGACAATTGTGGATGTTGCTGATGATCTTAGTTGGGGTTTGTTTTATTATAGCGGTGCTGCTCGAGCTGCAGGACAATGTTATACCGGTGCTGTGCTTGTCAGTCCGGATGGGGCATACCCAAATGATGTGCATAGGACGAGGTTAATTTCTGCTCTAGACAAGTGTGGAATTAAAGAGTGGGAGCTGTACACCGTTAATAATGCTTCATGTTTAGGTCCTCCATTGGGAATTCCCGAGGGTTCAAGTTTGCATTCAGTGATACAGGTTAAGGATCAGCAAGGGGCGTCCTTGTTGGGTTTGGCGGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3479

Protein Analysis

544

Amino Acids

60.45

Weight (kDa)

8.42

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VDE PF07137 257 - 507 5.8e-27 VDE lipocalin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 701
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 279
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 1423
AclWI GGATC 7 cut(s) 628, 628, 666, 812, 825, 970, 1599
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 5 cut(s) 184, 391, 520, 1010, 1548
AcuI CTGAAG 3 cut(s) 9, 755, 1065
AcyI GRCGYC 1 cut(s) 1604
AfaI GTAC 5 cut(s) 801, 1139, 1181, 1200, 1508
AfiI CCNNNNNNNGG 4 cut(s) 160, 166, 279, 1544
AgeI ACCGGT 2 cut(s) 220, 1403
AleI CACNNNNGTG 1 cut(s) 652
AluBI AGCT 8 cut(s) 23, 83, 588, 771, 1115, 1144, 1385, 1504
AluI AGCT 8 cut(s) 23, 83, 588, 771, 1115, 1144, 1385, 1504
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 7 cut(s) 628, 628, 666, 812, 825, 970, 1599
Ama87I CYCGRG 2 cut(s) 1380, 1553
Aor13HI TCCGGA 1 cut(s) 1423
AoxI GGCC 3 cut(s) 244, 791, 1161
ApeKI GCWGC 8 cut(s) 20, 80, 89, 92, 771, 858, 1376, 1385
ApoI RAATTY 5 cut(s) 184, 391, 520, 1010, 1548
ArsI GACNNNNNNTTYG 2 cut(s) 463, 495
AsiGI ACCGGT 2 cut(s) 220, 1403
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 791, 1535
AsuC2I CCSGG 2 cut(s) 161, 1266
AsuHPI GGTGA 3 cut(s) 211, 235, 439
AsuII TTCGAA 1 cut(s) 490
AvaI CYCGRG 2 cut(s) 1380, 1553
AvaII GGWCC 1 cut(s) 1535
AxyI CCTNAGG 1 cut(s) 795
BaeGI GKGCMC 1 cut(s) 154
BamHI GGATCC 1 cut(s) 817
BanI GGYRCC 1 cut(s) 251
BbvCI CCTCAGC 1 cut(s) 257
BbvI GCAGC 8 cut(s) 32, 92, 101, 104, 758, 870, 1363, 1372
BccI CCATC 2 cut(s) 1213, 1421
BceAI ACGGC 2 cut(s) 219, 371
BclI TGATCA 2 cut(s) 373, 1120
BcnI CCSGG 2 cut(s) 161, 1266
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 917, 1475
BfmI CTRYAG 1 cut(s) 1386
BglII AGATCT 1 cut(s) 1048
BisI GCNGC 8 cut(s) 21, 81, 90, 93, 772, 859, 1377, 1386
BlsI GCNGC 8 cut(s) 22, 82, 91, 94, 773, 860, 1378, 1387
Bme1390I CCNGG 2 cut(s) 161, 1266
Bme18I GGWCC 1 cut(s) 1535
BmeT110I CYCGRG 2 cut(s) 1380, 1553
BmgT120I GGNCC 2 cut(s) 791, 1535
BmiI GGNNCC 2 cut(s) 253, 819
BmrFI CCNGG 2 cut(s) 161, 1266
BmrI ACTGGG 1 cut(s) 857
BmsI GCATC 1 cut(s) 958
BmuI ACTGGG 1 cut(s) 857
BpmI CTGGAG 1 cut(s) 1246
Bpu10I CCTNAGC 2 cut(s) 257, 767
Bpu14I TTCGAA 1 cut(s) 490
BpuEI CTTGAG 1 cut(s) 251
BpuMI CCSGG 2 cut(s) 161, 1266
BsaHI GRCGYC 1 cut(s) 1604
BsaJI CCNNGG 7 cut(s) 159, 247, 278, 564, 1002, 1265, 1554
BsaWI WCCGGW 3 cut(s) 220, 1403, 1423
BsaXI ACNNNNNCTCC 2 cut(s) 1075, 1105
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 166, 279, 1544
Bse118I RCCGGY 4 cut(s) 69, 95, 220, 1403
Bse1I ACTGG 3 cut(s) 722, 863, 1187
Bse21I CCTNAGG 1 cut(s) 795
Bse3DI GCAATG 1 cut(s) 891
BseAI TCCGGA 1 cut(s) 1423
BseDI CCNNGG 7 cut(s) 159, 247, 278, 564, 1002, 1265, 1554
BseGI GGATG 5 cut(s) 679, 973, 1336, 1432, 1630
BseLI CCNNNNNNNGG 4 cut(s) 160, 166, 279, 1544
BseMI GCAATG 1 cut(s) 891
BseMII CTCAG 3 cut(s) 271, 758, 809
BseNI ACTGG 3 cut(s) 722, 863, 1187
BseRI GAGGAG 2 cut(s) 224, 1243
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 8 cut(s) 32, 92, 101, 104, 758, 870, 1363, 1372
BseYI CCCAGC 1 cut(s) 1111
BsgI GTGCAG 1 cut(s) 877
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 3 cut(s) 246, 793, 1163
BshNI GGYRCC 1 cut(s) 251
BshTI ACCGGT 2 cut(s) 220, 1403
BsiHKCI CYCGRG 2 cut(s) 1380, 1553
BsiSI CCGG 7 cut(s) 70, 96, 161, 221, 1265, 1404, 1424
BslFI GGGAC 2 cut(s) 710, 1282
BslI CCNNNNNNNGG 4 cut(s) 160, 166, 279, 1544
BsmAI GTCTC 1 cut(s) 206
BsmFI GGGAC 2 cut(s) 710, 1282
BsmI GAATGC 1 cut(s) 1570
BsnI GGCC 3 cut(s) 246, 793, 1163
BsoBI CYCGRG 2 cut(s) 1380, 1553
Bsp119I TTCGAA 1 cut(s) 490
Bsp1286I GDGCHC 1 cut(s) 154
Bsp13I TCCGGA 1 cut(s) 1423
Bsp1407I TGTACA 1 cut(s) 1506
Bsp19I CCATGG 1 cut(s) 1002
BspANI GGCC 3 cut(s) 246, 793, 1163
BspCNI CTCAG 3 cut(s) 270, 759, 808
BspEI TCCGGA 1 cut(s) 1423
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 253, 819
BspMAI CTGCAG 1 cut(s) 1390
BspPI GGATC 7 cut(s) 628, 628, 666, 812, 825, 970, 1599
BspT104I TTCGAA 1 cut(s) 490
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 891
BsrFI RCCGGY 4 cut(s) 69, 95, 220, 1403
BsrGI TGTACA 1 cut(s) 1506
BsrI ACTGG 3 cut(s) 722, 863, 1187
BssAI RCCGGY 4 cut(s) 69, 95, 220, 1403
BssECI CCNNGG 7 cut(s) 159, 247, 278, 564, 1002, 1265, 1554
BssNI GRCGYC 1 cut(s) 1604
BssT1I CCWWGG 4 cut(s) 247, 278, 564, 1002
Bst4CI ACNGT 5 cut(s) 149, 617, 643, 990, 1513
Bst6I CTCTTC 1 cut(s) 279
BstACI GRCGYC 1 cut(s) 1604
BstAUI TGTACA 1 cut(s) 1506
BstBI TTCGAA 1 cut(s) 490
BstC8I GCNNGC 1 cut(s) 791
BstDEI CTNAG 5 cut(s) 257, 743, 767, 795, 1346
BstDSI CCRYGG 1 cut(s) 1002
BstF5I GGATG 5 cut(s) 679, 973, 1336, 1432, 1630
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 194
BstMAI GTCTC 1 cut(s) 206
BstMWI GCNNNNNNNGC 6 cut(s) 17, 86, 89, 258, 1382, 1385
BstSCI CCNGG 2 cut(s) 159, 1264
BstSFI CTRYAG 1 cut(s) 1386
BstSLI GKGCMC 1 cut(s) 154
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 8 cut(s) 32, 92, 101, 104, 758, 870, 1363, 1372
BstX2I RGATCY 3 cut(s) 817, 975, 1048
BstYI RGATCY 3 cut(s) 817, 975, 1048
Bsu36I CCTNAGG 1 cut(s) 795
BsuRI GGCC 3 cut(s) 246, 793, 1163
BtgI CCRYGG 1 cut(s) 1002
BtsCI GGATG 5 cut(s) 679, 973, 1336, 1432, 1630
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 3 cut(s) 300, 622, 1581
Cac8I GCNNGC 1 cut(s) 791
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 4 cut(s) 69, 95, 220, 1403
Cfr13I GGNCC 2 cut(s) 791, 1535
CseI GACGC 1 cut(s) 1593
Csp6I GTAC 5 cut(s) 800, 1138, 1180, 1199, 1507
CspAI ACCGGT 2 cut(s) 220, 1403
CviAII CATG 5 cut(s) 114, 292, 775, 1003, 1527
CviQI GTAC 5 cut(s) 800, 1138, 1180, 1199, 1507
DdeI CTNAG 5 cut(s) 257, 743, 767, 795, 1346
EaeI YGGCCR 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EciI GGCGGA 2 cut(s) 221, 1249
Eco130I CCWWGG 4 cut(s) 247, 278, 564, 1002
Eco47I GGWCC 1 cut(s) 1535
Eco57I CTGAAG 3 cut(s) 9, 755, 1065
Eco81I CCTNAGG 1 cut(s) 795
Eco88I CYCGRG 2 cut(s) 1380, 1553
EcoO109I RGGNCCY 1 cut(s) 1535
EcoRI GAATTC 2 cut(s) 184, 1548
EcoT14I CCWWGG 4 cut(s) 247, 278, 564, 1002
ErhI CCWWGG 4 cut(s) 247, 278, 564, 1002
FaeI CATG 5 cut(s) 117, 295, 778, 1006, 1530
FaqI GGGAC 2 cut(s) 710, 1282
FatI CATG 5 cut(s) 113, 291, 774, 1002, 1526
FauI CCCGC 1 cut(s) 782
FauNDI CATATG 1 cut(s) 334
FbaI TGATCA 2 cut(s) 373, 1120
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 8 cut(s) 21, 81, 90, 93, 772, 859, 1377, 1386
FokI GGATG 4 cut(s) 686, 980, 1343, 1439
Fsp4HI GCNGC 8 cut(s) 21, 81, 90, 93, 772, 859, 1377, 1386
FspBI CTAG 2 cut(s) 917, 1475
GlaI GCGC 1 cut(s) 193
GluI GCNGC 8 cut(s) 21, 81, 90, 93, 772, 859, 1377, 1386
GsaI CCCAGC 1 cut(s) 1115
GsuI CTGGAG 1 cut(s) 1246
HaeIII GGCC 3 cut(s) 246, 793, 1163
HapII CCGG 7 cut(s) 70, 96, 161, 221, 1265, 1404, 1424
HgaI GACGC 1 cut(s) 1593
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 1604
Hin1II CATG 5 cut(s) 117, 295, 778, 1006, 1530
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HindIII AAGCTT 1 cut(s) 586
HinfI GANTC 7 cut(s) 452, 466, 509, 739, 850, 907, 1245
HpaII CCGG 7 cut(s) 70, 96, 161, 221, 1265, 1404, 1424
HphI GGTGA 3 cut(s) 211, 235, 439
Hpy166II GTNNAC 3 cut(s) 273, 325, 1509
Hpy188I TCNGA 5 cut(s) 373, 483, 519, 625, 831
Hpy8I GTNNAC 3 cut(s) 273, 325, 1509
HpyAV CCTTC 2 cut(s) 1015, 1153
HpyCH4III ACNGT 5 cut(s) 149, 617, 643, 990, 1513
HpyCH4IV ACGT 1 cut(s) 412
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 86, 89, 258, 1382, 1385
HpyF3I CTNAG 5 cut(s) 257, 743, 767, 795, 1346
HpySE526I ACGT 1 cut(s) 412
Hsp92I GRCGYC 1 cut(s) 1604
Hsp92II CATG 5 cut(s) 117, 295, 778, 1006, 1530
HspAI GCGC 1 cut(s) 192
Kpn2I TCCGGA 1 cut(s) 1423
Ksp22I TGATCA 2 cut(s) 373, 1120
LmnI GCTCC 2 cut(s) 300, 1501
Lsp1109I GCAGC 8 cut(s) 32, 92, 101, 104, 758, 870, 1363, 1372
LweI GCATC 1 cut(s) 958
MaeI CTAG 2 cut(s) 917, 1475
MaeII ACGT 1 cut(s) 412
MaeIII GTNAC 3 cut(s) 199, 439, 878
MboII GAAGA 5 cut(s) 95, 296, 748, 1058, 1228
MfeI CAATTG 1 cut(s) 1323
MflI RGATCY 3 cut(s) 817, 975, 1048
MhlI GDGCHC 1 cut(s) 154
MlyI GAGTC 3 cut(s) 503, 916, 1254
MmeI TCCRAC 2 cut(s) 112, 809
MroI TCCGGA 1 cut(s) 1423
MseI TTAA 8 cut(s) 174, 459, 705, 1008, 1463, 1491, 1515, 1587
MslI CAYNNNNRTG 2 cut(s) 652, 841
MspI CCGG 7 cut(s) 70, 96, 161, 221, 1265, 1404, 1424
MspR9I CCNGG 2 cut(s) 161, 1266
MunI CAATTG 1 cut(s) 1323
Mva1269I GAATGC 1 cut(s) 1570
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 6 cut(s) 17, 86, 89, 258, 1382, 1385
NciI CCSGG 2 cut(s) 161, 1266
NcoI CCATGG 1 cut(s) 1002
NdeI CATATG 1 cut(s) 334
NlaIII CATG 5 cut(s) 117, 295, 778, 1006, 1530
NlaIV GGNNCC 2 cut(s) 253, 819
NmuCI GTSAC 1 cut(s) 199
NspV TTCGAA 1 cut(s) 490
OliI CACNNNNGTG 1 cut(s) 652
PaeR7I CTCGAG 1 cut(s) 1380
PctI GAATGC 1 cut(s) 1570
PfeI GAWTC 4 cut(s) 452, 466, 739, 850
PflMI CCANNNNNTGG 1 cut(s) 279
PinAI ACCGGT 2 cut(s) 220, 1403
PkrI GCNGC 8 cut(s) 22, 82, 91, 94, 773, 860, 1378, 1387
PleI GAGTC 3 cut(s) 503, 915, 1253
PpsI GAGTC 3 cut(s) 503, 915, 1253
PpuMI RGGWCCY 1 cut(s) 1535
PsiI TTATAA 1 cut(s) 701
Psp5II RGGWCCY 1 cut(s) 1535
PspFI CCCAGC 1 cut(s) 1111
PspN4I GGNNCC 2 cut(s) 253, 819
PspPI GGNCC 2 cut(s) 791, 1535
PspPPI RGGWCCY 1 cut(s) 1535
PspXI VCTCGAGB 1 cut(s) 1380
PstI CTGCAG 1 cut(s) 1390
PsuI RGATCY 3 cut(s) 817, 975, 1048
RsaI GTAC 5 cut(s) 801, 1139, 1181, 1200, 1508
RsaNI GTAC 5 cut(s) 800, 1138, 1180, 1199, 1507
RseI CAYNNNNRTG 2 cut(s) 652, 841
SaqAI TTAA 8 cut(s) 174, 459, 705, 1008, 1463, 1491, 1515, 1587
SatI GCNGC 8 cut(s) 21, 81, 90, 93, 772, 859, 1377, 1386
Sau96I GGNCC 2 cut(s) 791, 1535
SchI GAGTC 3 cut(s) 503, 916, 1254
ScrFI CCNGG 2 cut(s) 161, 1266
SduI GDGCHC 1 cut(s) 154
SfaNI GCATC 1 cut(s) 958
SfcI CTRYAG 1 cut(s) 1386
Sfr274I CTCGAG 1 cut(s) 1380
SfuI TTCGAA 1 cut(s) 490
SgrAI CRCCGGYG 1 cut(s) 220
SinI GGWCC 1 cut(s) 1535
SlaI CTCGAG 1 cut(s) 1380
SmiMI CAYNNNNRTG 2 cut(s) 652, 841
SmlI CTYRAG 2 cut(s) 266, 1380
SmoI CTYRAG 2 cut(s) 266, 1380
SspI AATATT 1 cut(s) 487
SspMI CTAG 2 cut(s) 917, 1475
StyD4I CCNGG 2 cut(s) 159, 1264
StyI CCWWGG 4 cut(s) 247, 278, 564, 1002
TaaI ACNGT 5 cut(s) 149, 617, 643, 990, 1513
TaiI ACGT 1 cut(s) 415
TaqI TCGA 5 cut(s) 188, 490, 1243, 1307, 1381
TatI WGTACW 1 cut(s) 1506
TfiI GAWTC 4 cut(s) 452, 466, 739, 850
Tru1I TTAA 8 cut(s) 174, 459, 705, 1008, 1463, 1491, 1515, 1587
Tru9I TTAA 8 cut(s) 174, 459, 705, 1008, 1463, 1491, 1515, 1587
TscAI CASTG 3 cut(s) 307, 622, 1581
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 8 cut(s) 20, 80, 89, 92, 771, 858, 1376, 1385
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 5 cut(s) 51, 95, 351, 626, 1515
TspGWI ACGGA 2 cut(s) 416, 686
TspRI CASTG 3 cut(s) 307, 622, 1581
Van91I CCANNNNNTGG 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 1535
XapI RAATTY 5 cut(s) 184, 391, 520, 1010, 1548
XbaI TCTAGA 2 cut(s) 916, 1474
XhoI CTCGAG 1 cut(s) 1380
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 2 cut(s) 917, 1475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.