FvH4_1g24820

At4g29660-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014011
Physical Location & Seq
Reverse (-)
16599239 .. 16600307
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24820.t1

Sequence Viewer

Length: 300 bp
ATGGCGAGCTACATGTGGAGGAAATACGCAGACTATGTGTACACAAAATGGGAAAGAATGATTCTTTGGAACATGGTCAAGCCCTACACCCGAACCAAGTATTTTACTCCTCTCGTCACTATCTACGTCGCTGCTTTCTACACCGGAGTCAACGCCTCCGCCGTCACTGAACAGCTCTACAAGGAAAAATATTGGGAAGATCATCCTGGCCATCAGGTCCCTCTTATGAAGCCAAAGTTTTATGGAGGACCTTGGAAGGTAGAGAGAGGAGAGGTTCCAGCCTCTCTCCCAACCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

11.79

Weight (kDa)

9.4

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29660
fragaria_vesca FvH4_1g07240 FvH4_1g24820
malus_domestica MD02G1077000.v1.1 MD15G1205700.v1.1
prunus_persica Prupe.7G211400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0093151
rosa_laevigata RLG00000015678
rosa_multiflora Rmu_sc0003724.1_g000004
rosa_roxburghii Rroxscaffold_2G00110150 Rroxscaffold_7G00185150
rosa_rugosa Rorug02G0033200
rosa_samantha Rh2AG078900 Rh2BG080200 Rh2CG082400 Rh2DG077900
rosa_wichuraiana Rw2G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 208
AfaI GTAC 1 cut(s) 41
AflIII ACRYGT 1 cut(s) 12
AjnI CCWGG 1 cut(s) 205
AjuI GAANNNNNNNTTGG 2 cut(s) 49, 81
AluBI AGCT 2 cut(s) 9, 175
AluI AGCT 2 cut(s) 9, 175
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 217, 248
AvaII GGWCC 2 cut(s) 217, 248
BalI TGGCCA 1 cut(s) 210
BbvI GCAGC 1 cut(s) 118
BccI CCATC 1 cut(s) 219
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 207
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 2 cut(s) 217, 248
BmgT120I GGNCC 2 cut(s) 217, 248
BmiI GGNNCC 2 cut(s) 219, 276
BmrFI CCNGG 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 251
BsaWI WCCGGW 1 cut(s) 143
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 251
BseGI GGATG 1 cut(s) 202
BseRI GAGGAG 2 cut(s) 99, 282
BseXI GCAGC 1 cut(s) 118
BshFI GGCC 1 cut(s) 210
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 1 cut(s) 210
Bsp1407I TGTACA 1 cut(s) 39
Bsp143I GATC 1 cut(s) 199
BspACI CCGC 1 cut(s) 159
BspANI GGCC 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 219, 276
BsrGI TGTACA 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 251
BssMI GATC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 207
BstAUI TGTACA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 7
BstF5I GGATG 1 cut(s) 202
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 118
BsuRI GGCC 1 cut(s) 210
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 217, 248
Csp6I GTAC 1 cut(s) 40
CviAII CATG 2 cut(s) 13, 73
CviJI RGCY 6 cut(s) 9, 82, 175, 210, 232, 281
CviKI_1 RGCY 6 cut(s) 9, 82, 175, 210, 232, 281
CviQI GTAC 1 cut(s) 40
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
EaeI YGGCCR 1 cut(s) 208
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 251
Eco47I GGWCC 2 cut(s) 217, 248
EcoO109I RGGNCCY 2 cut(s) 217, 248
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 251
ErhI CCWWGG 1 cut(s) 251
FaeI CATG 2 cut(s) 16, 76
FaiI YATR 6 cut(s) 14, 36, 74, 227, 243, 298
FaqI GGGAC 1 cut(s) 203
FatI CATG 2 cut(s) 12, 72
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 132
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 144
Hin1II CATG 2 cut(s) 16, 76
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 2 cut(s) 61, 147
HpaII CCGG 1 cut(s) 144
Hpy166II GTNNAC 3 cut(s) 40, 42, 151
Hpy8I GTNNAC 3 cut(s) 40, 42, 151
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 16, 76
Kzo9I GATC 1 cut(s) 199
LpnPI CCDG 5 cut(s) 157, 192, 200, 219, 291
Lsp1109I GCAGC 1 cut(s) 118
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 115, 163
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 1 cut(s) 209
MlsI TGGCCA 1 cut(s) 210
MluNI TGGCCA 1 cut(s) 210
MlyI GAGTC 1 cut(s) 156
MnlI CCTC 8 cut(s) 12, 120, 166, 231, 239, 260, 265, 292
Mox20I TGGCCA 1 cut(s) 210
MscI TGGCCA 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 210
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
NdeII GATC 1 cut(s) 199
NlaIII CATG 2 cut(s) 16, 76
NlaIV GGNNCC 2 cut(s) 219, 276
NmuCI GTSAC 2 cut(s) 115, 163
NspI RCATGY 1 cut(s) 16
PciI ACATGT 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 1 cut(s) 61
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PpuMI RGGWCCY 2 cut(s) 217, 248
PscI ACATGT 1 cut(s) 12
Psp5II RGGWCCY 2 cut(s) 217, 248
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 219, 276
PspPI GGNCC 2 cut(s) 217, 248
PspPPI RGGWCCY 2 cut(s) 217, 248
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 1 cut(s) 199
Sau96I GGNCC 2 cut(s) 217, 248
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 1 cut(s) 207
SetI ASST 7 cut(s) 11, 129, 177, 219, 253, 261, 276
SinI GGWCC 2 cut(s) 217, 248
SsiI CCGC 1 cut(s) 159
SspI AATATT 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 251
TaiI ACGT 1 cut(s) 129
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 1 cut(s) 61
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 2 cut(s) 115, 163
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 2 cut(s) 115, 163
TspDTI ATGAA 1 cut(s) 242
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 2 cut(s) 217, 248
XceI RCATGY 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.