MD02G1077000.v1.1

At4g29660-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014011
Physical Location & Seq
Reverse (-)
6079650 .. 6081193
1544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1077000.v1.1.491

Sequence Viewer

Length: 291 bp
ATGGCGAGCTATCTATGGAGGAAATACGCAGATTACGTTTACACAAAGTGGGAAAGAATGATTCTTTGGAACATGGTGGAGCCCTACAGCAGACCCAAATCGTTCACCCCGTTGGTCACCATCTACGTCGCCGCTTTCTACACTGGTGTGATTGGTTCTGCTATCACTGAGCAGCTTTACAAGGAAAAATACTGGGAAGAACATCCCGGGCAGGCAGTGCCTCTTATGAAGCCAAAGTTTTACGGAGGACCGTGGAGGGTACAGCAAGGCGAGGTCCCAGCCACTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

11.36

Weight (kDa)

9.13

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29660
fragaria_vesca FvH4_1g07240 FvH4_1g24820
malus_domestica MD02G1077000.v1.1 MD15G1205700.v1.1
prunus_persica Prupe.7G211400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0093151
rosa_laevigata RLG00000015678
rosa_multiflora Rmu_sc0003724.1_g000004
rosa_roxburghii Rroxscaffold_2G00110150 Rroxscaffold_7G00185150
rosa_rugosa Rorug02G0033200
rosa_samantha Rh2AG078900 Rh2BG080200 Rh2CG082400 Rh2DG077900
rosa_wichuraiana Rw2G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 132
AdeI CACNNNGTG 2 cut(s) 48, 288
AfaI GTAC 1 cut(s) 261
AjuI GAANNNNNNNTTGG 2 cut(s) 49, 81
AleI CACNNNNGTG 1 cut(s) 146
AluBI AGCT 2 cut(s) 9, 175
AluI AGCT 2 cut(s) 9, 175
Ama87I CYCGRG 1 cut(s) 206
ApeKI GCWGC 1 cut(s) 172
AspS9I GGNCC 2 cut(s) 248, 274
AsuC2I CCSGG 2 cut(s) 207, 208
AsuHPI GGTGA 2 cut(s) 97, 109
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 2 cut(s) 248, 274
BanII GRGCYC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 184
BccI CCATC 1 cut(s) 128
BcnI CCSGG 2 cut(s) 207, 208
BfmI CTRYAG 1 cut(s) 85
BisI GCNGC 2 cut(s) 132, 173
BlsI GCNGC 2 cut(s) 133, 174
Bme1390I CCNGG 2 cut(s) 207, 208
Bme18I GGWCC 2 cut(s) 248, 274
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 248, 274
BmiI GGNNCC 2 cut(s) 81, 276
BmrFI CCNGG 2 cut(s) 207, 208
BmrI ACTGGG 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 202
BpuMI CCSGG 2 cut(s) 207, 208
BsaJI CCNNGG 2 cut(s) 206, 251
Bse1I ACTGG 2 cut(s) 148, 197
BseDI CCNNGG 2 cut(s) 206, 251
BseGI GGATG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 2 cut(s) 148, 197
BseXI GCAGC 1 cut(s) 184
BseYI CCCAGC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 84
BspACI CCGC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 81, 276
BsrI ACTGG 2 cut(s) 148, 197
BssECI CCNNGG 2 cut(s) 206, 251
Bst4CI ACNGT 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 217
BstC8I GCNNGC 2 cut(s) 7, 213
BstDEI CTNAG 2 cut(s) 168, 285
BstDSI CCRYGG 1 cut(s) 251
BstEII GGTNACC 1 cut(s) 115
BstF5I GGATG 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstPI GGTNACC 1 cut(s) 115
BstSCI CCNGG 2 cut(s) 205, 206
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 184
BtgI CCRYGG 1 cut(s) 251
BtsCI GGATG 1 cut(s) 202
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 3 cut(s) 141, 165, 222
Cac8I GCNNGC 2 cut(s) 7, 213
Cfr13I GGNCC 2 cut(s) 248, 274
Cfr9I CCCGGG 1 cut(s) 206
Csp6I GTAC 1 cut(s) 260
CviAII CATG 1 cut(s) 73
CviJI RGCY 5 cut(s) 9, 82, 175, 232, 281
CviKI_1 RGCY 5 cut(s) 9, 82, 175, 232, 281
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 2 cut(s) 168, 285
DraIII CACNNNGTG 2 cut(s) 48, 288
Eco24I GRGCYC 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 248, 274
Eco88I CYCGRG 1 cut(s) 206
Eco91I GGTNACC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 274
EcoO65I GGTNACC 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 84
FaeI CATG 1 cut(s) 76
FaiI YATR 3 cut(s) 16, 74, 227
FaqI GGGAC 1 cut(s) 260
FatI CATG 1 cut(s) 72
Fnu4HI GCNGC 2 cut(s) 132, 173
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 132, 173
GluI GCNGC 2 cut(s) 132, 173
GsaI CCCAGC 1 cut(s) 281
HapII CCGG 1 cut(s) 207
Hin1II CATG 1 cut(s) 76
HinfI GANTC 1 cut(s) 61
HpaII CCGG 1 cut(s) 207
HphI GGTGA 2 cut(s) 97, 109
Hpy166II GTNNAC 2 cut(s) 40, 105
Hpy8I GTNNAC 2 cut(s) 40, 105
Hpy99I CGWCG 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4IV ACGT 2 cut(s) 36, 126
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 2 cut(s) 168, 285
HpySE526I ACGT 2 cut(s) 36, 126
Hsp92II CATG 1 cut(s) 76
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 4 cut(s) 129, 178, 197, 220
Lsp1109I GCAGC 1 cut(s) 184
MaeII ACGT 2 cut(s) 36, 126
MaeIII GTNAC 1 cut(s) 115
MboII GAAGA 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 84
MnlI CCTC 5 cut(s) 12, 231, 239, 249, 265
MslI CAYNNNNRTG 1 cut(s) 146
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 207, 208
MwoI GCNNNNNNNGC 1 cut(s) 217
NciI CCSGG 2 cut(s) 207, 208
NlaIII CATG 1 cut(s) 76
NlaIV GGNNCC 2 cut(s) 81, 276
NmuCI GTSAC 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 146
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 107
PfeI GAWTC 1 cut(s) 61
PkrI GCNGC 2 cut(s) 133, 174
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
PspEI GGTNACC 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 81, 276
PspPI GGNCC 2 cut(s) 248, 274
PspPPI RGGWCCY 1 cut(s) 274
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
RseI CAYNNNNRTG 1 cut(s) 146
SatI GCNGC 2 cut(s) 132, 173
Sau96I GGNCC 2 cut(s) 248, 274
ScrFI CCNGG 2 cut(s) 207, 208
SduI GDGCHC 1 cut(s) 84
SetI ASST 5 cut(s) 11, 39, 129, 177, 276
SfcI CTRYAG 1 cut(s) 85
SinI GGWCC 2 cut(s) 248, 274
SmaI CCCGGG 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 146
SsiI CCGC 1 cut(s) 132
StyD4I CCNGG 2 cut(s) 205, 206
TaaI ACNGT 1 cut(s) 252
TaiI ACGT 2 cut(s) 39, 129
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 61
TscAI CASTG 3 cut(s) 148, 172, 222
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 242
TspGWI ACGGA 1 cut(s) 258
TspMI CCCGGG 1 cut(s) 206
TspRI CASTG 3 cut(s) 148, 172, 222
VpaK11BI GGWCC 2 cut(s) 248, 274
XmaI CCCGGG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.