Rmu_sc0003724.1_g000004

At4g29660-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003724.1
OG0014011
Physical Location & Seq
Reverse (-)
16930 .. 18247
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003724.1_g000004.1.cds

Sequence Viewer

Length: 228 bp
atggtggagccctacaccaggcccaaatcgtttacgcctctcgtcaccatctacgtcgccgctttctacactggagtcgtcgcctctgccatcactgaacagctctacaaggaaaaatactgggaagatcatcctggccatgcagtcccacttatgaagcccaagttttatggaggaccttggaaggtagagagaggagaggttccggccgctctcccacccccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

75

Amino Acids

8.45

Weight (kDa)

7.99

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29660
fragaria_vesca FvH4_1g07240 FvH4_1g24820
malus_domestica MD02G1077000.v1.1 MD15G1205700.v1.1
prunus_persica Prupe.7G211400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0093151
rosa_laevigata RLG00000015678
rosa_multiflora Rmu_sc0003724.1_g000004
rosa_roxburghii Rroxscaffold_2G00110150 Rroxscaffold_7G00185150
rosa_rugosa Rorug02G0033200
rosa_samantha Rh2AG078900 Rh2BG080200 Rh2CG082400 Rh2DG077900
rosa_wichuraiana Rw2G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 212
AciI CCGC 2 cut(s) 60, 210
AcoI YGGCCR 2 cut(s) 136, 207
AfiI CCNNNNNNNGG 1 cut(s) 18
AjnI CCWGG 2 cut(s) 17, 133
AluBI AGCT 1 cut(s) 103
AluI AGCT 1 cut(s) 103
AoxI GGCC 3 cut(s) 20, 136, 207
AspS9I GGNCC 2 cut(s) 21, 176
AsuHPI GGTGA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 176
BalI TGGCCA 1 cut(s) 138
BanII GRGCYC 1 cut(s) 12
BccI CCATC 2 cut(s) 56, 98
BciT130I CCWGG 2 cut(s) 19, 135
BisI GCNGC 2 cut(s) 60, 210
BlsI GCNGC 2 cut(s) 61, 211
Bme1390I CCNGG 2 cut(s) 19, 135
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 2 cut(s) 21, 176
BmiI GGNNCC 2 cut(s) 9, 204
BmrFI CCNGG 2 cut(s) 19, 135
BmrI ACTGGG 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 179
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 2 cut(s) 76, 125
BseBI CCWGG 2 cut(s) 19, 135
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 76, 125
BseRI GAGGAG 1 cut(s) 210
BseX3I CGGCCG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 22, 138, 209
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 3 cut(s) 22, 138, 209
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 1 cut(s) 127
BspACI CCGC 2 cut(s) 60, 210
BspANI GGCC 3 cut(s) 22, 138, 209
BspLI GGNNCC 2 cut(s) 9, 204
BsrBI CCGCTC 1 cut(s) 212
BsrI ACTGG 2 cut(s) 76, 125
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 2 cut(s) 19, 135
BstENI CCTNNNNNAGG 1 cut(s) 16
BstF5I GGATG 1 cut(s) 130
BstKTI GATC 1 cut(s) 130
BstMBI GATC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 210
BstNI CCWGG 2 cut(s) 19, 135
BstSCI CCNGG 2 cut(s) 17, 133
BstZI CGGCCG 1 cut(s) 207
BsuRI GGCC 3 cut(s) 22, 138, 209
BtsCI GGATG 1 cut(s) 130
BtsIMutI CAGTG 2 cut(s) 69, 93
Cfr13I GGNCC 2 cut(s) 21, 176
CviAII CATG 1 cut(s) 140
CviJI RGCY 6 cut(s) 10, 22, 103, 138, 160, 209
CviKI_1 RGCY 6 cut(s) 10, 22, 103, 138, 160, 209
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 136, 207
EagI CGGCCG 1 cut(s) 207
EclXI CGGCCG 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 176
Eco52I CGGCCG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 17, 133
EcoT14I CCWWGG 1 cut(s) 179
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 1 cut(s) 143
FaiI YATR 4 cut(s) 141, 155, 171, 226
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 60, 210
FokI GGATG 1 cut(s) 117
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 2 cut(s) 60, 210
GluI GCNGC 2 cut(s) 60, 210
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 3 cut(s) 22, 138, 209
HapII CCGG 1 cut(s) 206
Hin1II CATG 1 cut(s) 143
HinfI GANTC 1 cut(s) 75
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 1 cut(s) 33
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 2 cut(s) 59, 83
HpyAV CCTTC 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 143
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 1 cut(s) 143
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 7
LpnPI CCDG 7 cut(s) 4, 31, 57, 106, 120, 147, 219
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 129
MbiI CCGCTC 1 cut(s) 212
MboI GATC 1 cut(s) 127
MboII GAAGA 1 cut(s) 137
MhlI GDGCHC 1 cut(s) 12
MlsI TGGCCA 1 cut(s) 138
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 1 cut(s) 84
MnlI CCTC 5 cut(s) 48, 94, 167, 188, 193
Mox20I TGGCCA 1 cut(s) 138
MscI TGGCCA 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 138
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 19, 135
MvaI CCWGG 2 cut(s) 19, 135
NdeII GATC 1 cut(s) 127
NlaIII CATG 1 cut(s) 143
NlaIV GGNNCC 2 cut(s) 9, 204
NmuCI GTSAC 1 cut(s) 43
PkrI GCNGC 2 cut(s) 61, 211
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 17, 133
PspGI CCWGG 2 cut(s) 17, 133
PspN4I GGNNCC 2 cut(s) 9, 204
PspPI GGNCC 2 cut(s) 21, 176
PspPPI RGGWCCY 1 cut(s) 176
SatI GCNGC 2 cut(s) 60, 210
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 2 cut(s) 21, 176
SchI GAGTC 1 cut(s) 84
ScrFI CCNGG 2 cut(s) 19, 135
SduI GDGCHC 1 cut(s) 12
SetI ASST 5 cut(s) 57, 105, 181, 189, 204
SinI GGWCC 1 cut(s) 176
SsiI CCGC 2 cut(s) 60, 210
StyD4I CCNGG 2 cut(s) 17, 133
StyI CCWWGG 1 cut(s) 179
TaiI ACGT 1 cut(s) 57
TauI GCSGC 2 cut(s) 62, 212
TscAI CASTG 2 cut(s) 76, 100
TseFI GTSAC 1 cut(s) 43
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 170
TspRI CASTG 2 cut(s) 76, 100
VpaK11BI GGWCC 1 cut(s) 176
XagI CCTNNNNNAGG 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.