FvH4_1g24890

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017232
Physical Location & Seq
Reverse (-)
16670328 .. 16673564
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24890.t2

Sequence Viewer

Length: 810 bp
ATGGCTGCTTGTGGTCCAGCACTGACTTTTATAGCAGCATGCTTTTTTGGGATGGAGTATGTGCACTGGACTGCTCGGAGCTTTCAGGCGAAGGTTTTAGGCATGATATTGATGGTGGGCGGAGGTTGTATCGTTGGATTTTATCACGGCTCCTCTATCAATATCATGCACACTTCTATTGGTACTCGGTTTGGAAGGACTGAGCGTGAACTTCGAGAGGACTGGTTTCGTGGTCCTCTTCTGGTGTTTGTGTCATTAGCTGCATCAGTTTGGTACAATCTTCAGATTAAGAAGGTGGCAGAGCAGGTAGGACCAGCCATATGGCTGAGTTTCTATATTTTTGCGTTGGGTACGGCTATGACTGTGCTAGTCGCTCTGGGATTAGAGGTGAGATCTCCACATGTATGGTATGTTATAGCAGACATCCGATTAGTCAGCTACATATATGCGGGCGTCGTAGTTTCTGGTCTTGCTTCATTCATCCAGATTACACTCACTAGAATGAGAGACCCTGTATTTGTTGCTGCTTTTAGCCCCATCTCATTGATATTTGTGATGGTGATGAGTCTATTAACATTGCGGGACGTCATACATATGGGGAGCATTTTAGGGGCGATATGCATCATGTTTGGGCTTCTTATGCTTCTGTGGGGAAAGGCGCATGATCCCACACCCCTAGTACTCCCTGTTCAGGAGATGACATATGTACCTCGGTCATCACCTTCAGCAGTGCCGTCGCTACAGCCACTGTCATCGCCACAGCTAGAGGCTGGAGAGCCAGATGGGCCAGGGCCAGAGCCAGAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

270

Amino Acids

29.37

Weight (kDa)

6.58

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 61 - 199 9e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24890 FvH4_1g24890
malus_domestica MD15G1135500.v1.1
prunus_persica Prupe.4G273500_v2.0.a1
pyrus_communis pycom15g12280
rosa_chinensis RchiOBHm_Chr2g0122551
rosa_laevigata RLG00000018686
rosa_multiflora Rmu_sc0003832.1_g000004
rosa_roxburghii Rroxscaffold_2G00121480
rosa_samantha Rh2AG298100 Rh2BG306900 Rh2CG285000 Rh2DG321700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 588
Acc36I ACCTGC 1 cut(s) 295
AciI CCGC 3 cut(s) 120, 449, 580
AclWI GGATC 1 cut(s) 659
AcuI CTGAAG 2 cut(s) 266, 708
AcyI GRCGYC 2 cut(s) 453, 585
AfaI GTAC 5 cut(s) 184, 275, 352, 681, 708
AfiI CCNNNNNNNGG 1 cut(s) 691
AflIII ACRYGT 1 cut(s) 400
AjnI CCWGG 1 cut(s) 787
AluBI AGCT 5 cut(s) 81, 260, 438, 763, 807
AluI AGCT 5 cut(s) 81, 260, 438, 763, 807
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 1 cut(s) 501
Alw44I GTGCAC 1 cut(s) 62
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 1 cut(s) 748
AoxI GGCC 2 cut(s) 785, 791
ApaLI GTGCAC 1 cut(s) 62
ApeKI GCWGC 4 cut(s) 5, 35, 260, 524
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 5 cut(s) 14, 233, 311, 785, 791
AsuHPI GGTGA 3 cut(s) 400, 571, 711
AvaII GGWCC 3 cut(s) 14, 233, 311
BaeGI GKGCMC 1 cut(s) 66
BaeI ACNNNNGTAYC 2 cut(s) 690, 723
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 3 cut(s) 47, 247, 511
BccI CCATC 5 cut(s) 46, 106, 545, 550, 776
BceAI ACGGC 3 cut(s) 163, 369, 718
BcgI CGANNNNNNTGC 2 cut(s) 717, 751
BciT130I CCWGG 1 cut(s) 789
BcoDI GTCTC 1 cut(s) 501
BfaI CTAG 4 cut(s) 368, 498, 677, 764
BfmI CTRYAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 295
BglI GCCNNNNNGGC 1 cut(s) 784
BglII AGATCT 1 cut(s) 392
BisI GCNGC 4 cut(s) 6, 36, 261, 525
BlsI GCNGC 4 cut(s) 7, 37, 262, 526
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 1 cut(s) 789
Bme18I GGWCC 3 cut(s) 14, 233, 311
BmgT120I GGNCC 5 cut(s) 14, 233, 311, 785, 791
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 789
BmsI GCATC 2 cut(s) 272, 630
BpmI CTGGAG 1 cut(s) 792
BsaBI GATNNNNATC 1 cut(s) 620
BsaHI GRCGYC 2 cut(s) 453, 585
BsaI GGTCTC 1 cut(s) 501
BsaJI CCNNGG 2 cut(s) 710, 788
Bsc4I CCNNNNNNNGG 1 cut(s) 691
Bse1I ACTGG 2 cut(s) 71, 227
Bse3DI GCAATG 1 cut(s) 575
Bse8I GATNNNNATC 1 cut(s) 620
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 710, 788
BseGI GGATG 3 cut(s) 57, 423, 480
BseJI GATNNNNATC 1 cut(s) 620
BseLI CCNNNNNNNGG 1 cut(s) 691
BseMI GCAATG 1 cut(s) 575
BseMII CTCAG 2 cut(s) 192, 317
BseNI ACTGG 2 cut(s) 71, 227
BseRI GAGGAG 1 cut(s) 142
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 3 cut(s) 47, 247, 511
BshFI GGCC 2 cut(s) 787, 793
BsiHKAI GWGCWC 1 cut(s) 66
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 691
BsmAI GTCTC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 596
BsnI GGCC 2 cut(s) 787, 793
Bso31I GGTCTC 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 2 cut(s) 392, 664
BspACI CCGC 3 cut(s) 120, 449, 580
BspANI GGCC 2 cut(s) 787, 793
BspCNI CTCAG 2 cut(s) 193, 318
BspLI GGNNCC 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 295
BspPI GGATC 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 501
BsrDI GCAATG 1 cut(s) 575
BsrI ACTGG 2 cut(s) 71, 227
BssECI CCNNGG 2 cut(s) 710, 788
BssMI GATC 2 cut(s) 392, 664
BssNI GRCGYC 2 cut(s) 453, 585
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 2 cut(s) 364, 750
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 2 cut(s) 453, 585
BstC8I GCNNGC 2 cut(s) 40, 451
BstDEI CTNAG 2 cut(s) 201, 326
BstF5I GGATG 3 cut(s) 57, 423, 480
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 2 cut(s) 395, 667
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 2 cut(s) 392, 664
BstMWI GCNNNNNNNGC 2 cut(s) 640, 784
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 2 cut(s) 42, 404
BstSCI CCNGG 1 cut(s) 787
BstSFI CTRYAG 1 cut(s) 740
BstSLI GKGCMC 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 47, 247, 511
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 2 cut(s) 321, 405
BstYI RGATCY 1 cut(s) 392
BsuRI GGCC 2 cut(s) 787, 793
BtgZI GCGATG 1 cut(s) 738
BtsCI GGATG 3 cut(s) 57, 423, 480
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 4 cut(s) 20, 64, 735, 746
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 2 cut(s) 40, 451
CaiI CAGNNNCTG 1 cut(s) 748
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 5 cut(s) 14, 233, 311, 785, 791
CseI GACGC 1 cut(s) 442
Csp6I GTAC 5 cut(s) 183, 274, 351, 680, 707
CviAII CATG 6 cut(s) 39, 103, 166, 401, 625, 662
CviQI GTAC 5 cut(s) 183, 274, 351, 680, 707
DdeI CTNAG 2 cut(s) 201, 326
DpnI GATC 2 cut(s) 394, 666
DpnII GATC 2 cut(s) 392, 664
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
EciI GGCGGA 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 501
Eco47I GGWCC 3 cut(s) 14, 233, 311
Eco57I CTGAAG 2 cut(s) 266, 708
EcoRII CCWGG 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 623
FaeI CATG 6 cut(s) 42, 106, 169, 404, 628, 665
FaqI GGGAC 1 cut(s) 596
FatI CATG 6 cut(s) 38, 102, 165, 400, 624, 661
FauI CCCGC 2 cut(s) 442, 573
FauNDI CATATG 3 cut(s) 320, 594, 703
Fnu4HI GCNGC 4 cut(s) 6, 36, 261, 525
FokI GGATG 3 cut(s) 64, 410, 467
Fsp4HI GCNGC 4 cut(s) 6, 36, 261, 525
FspBI CTAG 4 cut(s) 368, 498, 677, 764
GlaI GCGC 1 cut(s) 660
GluI GCNGC 4 cut(s) 6, 36, 261, 525
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 2 cut(s) 787, 793
HgaI GACGC 1 cut(s) 442
HhaI GCGC 1 cut(s) 661
Hin1I GRCGYC 2 cut(s) 453, 585
Hin1II CATG 6 cut(s) 42, 106, 169, 404, 628, 665
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HinfI GANTC 1 cut(s) 565
HphI GGTGA 3 cut(s) 400, 571, 711
Hpy166II GTNNAC 2 cut(s) 64, 209
Hpy188I TCNGA 3 cut(s) 78, 285, 428
Hpy188III TCNNGA 3 cut(s) 215, 484, 692
Hpy8I GTNNAC 2 cut(s) 64, 209
Hpy99I CGWCG 2 cut(s) 458, 739
HpyAV CCTTC 4 cut(s) 85, 189, 286, 732
HpyCH4III ACNGT 2 cut(s) 364, 750
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 4 cut(s) 64, 169, 263, 621
HpyF10VI GCNNNNNNNGC 2 cut(s) 640, 784
HpyF3I CTNAG 2 cut(s) 201, 326
HpySE526I ACGT 1 cut(s) 585
Hsp92I GRCGYC 2 cut(s) 453, 585
Hsp92II CATG 6 cut(s) 42, 106, 169, 404, 628, 665
HspAI GCGC 1 cut(s) 659
Kzo9I GATC 2 cut(s) 392, 664
LmnI GCTCC 3 cut(s) 78, 155, 600
Lsp1109I GCAGC 3 cut(s) 47, 247, 511
LweI GCATC 2 cut(s) 272, 630
MaeI CTAG 4 cut(s) 368, 498, 677, 764
MaeII ACGT 1 cut(s) 585
MalI GATC 2 cut(s) 394, 666
MboI GATC 2 cut(s) 392, 664
MboII GAAGA 2 cut(s) 230, 272
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 7 cut(s) 116, 163, 211, 246, 379, 720, 760
Mph1103I ATGCAT 1 cut(s) 623
MseI TTAA 2 cut(s) 288, 572
MslI CAYNNNNRTG 3 cut(s) 403, 500, 593
MspR9I CCNGG 1 cut(s) 789
MvaI CCWGG 1 cut(s) 789
MwoI GCNNNNNNNGC 2 cut(s) 640, 784
NdeI CATATG 3 cut(s) 320, 594, 703
NdeII GATC 2 cut(s) 392, 664
NlaIII CATG 6 cut(s) 42, 106, 169, 404, 628, 665
NlaIV GGNNCC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 2 cut(s) 42, 404
PaeI GCATGC 1 cut(s) 42
PciI ACATGT 1 cut(s) 400
PkrI GCNGC 4 cut(s) 7, 37, 262, 526
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
PscI ACATGT 1 cut(s) 400
Psp6I CCWGG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 5 cut(s) 14, 233, 311, 785, 791
PsrI GAACNNNNNNTAC 2 cut(s) 672, 704
PstNI CAGNNNCTG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 392
RsaI GTAC 5 cut(s) 184, 275, 352, 681, 708
RsaNI GTAC 5 cut(s) 183, 274, 351, 680, 707
RseI CAYNNNNRTG 3 cut(s) 403, 500, 593
SaqAI TTAA 2 cut(s) 288, 572
SatI GCNGC 4 cut(s) 6, 36, 261, 525
Sau3AI GATC 2 cut(s) 392, 664
Sau96I GGNCC 5 cut(s) 14, 233, 311, 785, 791
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 1 cut(s) 789
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 2 cut(s) 272, 630
SfcI CTRYAG 1 cut(s) 740
SinI GGWCC 3 cut(s) 14, 233, 311
SmiMI CAYNNNNRTG 3 cut(s) 403, 500, 593
SphI GCATGC 1 cut(s) 42
SsiI CCGC 3 cut(s) 120, 449, 580
SspMI CTAG 4 cut(s) 368, 498, 677, 764
StyD4I CCNGG 1 cut(s) 787
TaaI ACNGT 2 cut(s) 364, 750
TaiI ACGT 1 cut(s) 588
TaqI TCGA 1 cut(s) 214
TaqII GACCGA 1 cut(s) 702
TatI WGTACW 1 cut(s) 679
Tru1I TTAA 2 cut(s) 288, 572
Tru9I TTAA 2 cut(s) 288, 572
TscAI CASTG 4 cut(s) 27, 71, 735, 753
TseI GCWGC 4 cut(s) 5, 35, 260, 524
TspDTI ATGAA 2 cut(s) 465, 469
TspRI CASTG 4 cut(s) 27, 71, 735, 753
VneI GTGCAC 1 cut(s) 62
VpaK11BI GGWCC 3 cut(s) 14, 233, 311
XceI RCATGY 2 cut(s) 42, 404
XspI CTAG 4 cut(s) 368, 498, 677, 764
ZraI GACGTC 1 cut(s) 586
ZrmI AGTACT 1 cut(s) 681
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.