Prupe.4G273500_v2.0.a1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017232
Physical Location & Seq
Forward (+)
22662365 .. 22666352
3988 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G273500.1

Sequence Viewer

Length: 1128 bp
ATGGAGGGTCAACAACAAGTGATGGTTGTTGAAGAAATTGTGGAGGAGGGAGAACAACATGAGCTGGCTATTGAACAACAGCCAGCAAATGTTGTCATGAACTCGAACGAAGAAGAAGCTCAAGGACAATGGGGAAAAATAAAAAAGGTTGCCCCATTTATCATTGGGATGTTGATCCCAATGCTAATTGGAGGCAACTCTATCTTCACTAAAAGGGGATTGAAGGAGGGAATGAGCTTTAGTGTGTTTTTATTTTATAAAAACACACTAGCCTCCATTGTGTTACTTGTGGCTACATTGATTACCAGAACACCGTGGCCCAAAATGACGCGCTGGCTTGTCATTCGGATCATAGCATTGAGCGTACTCGAGCCGGTCTTGAGCCAGATTGCCATATTTTCTGGACTGAAATTCACACCAGCGAGTTTCTCTAGTTGTCTCATTGCCACTGGTCCAGCCTTGACATTAGTTGCCTCCTGGATTTTACAACTGGAGCACATTGCAATTAGTGAGAGGCGCAGCCAAGCAAAATTGCTTGGCATGGTGATGGTGATTACTGGAGGTTTGGTTGTCGGCCTGTATAATGGCCCAACCATCACAGTGTTGCAGCCCTCGCACATCAGCGGCCAAAATGTCGTTTCTCATCGGCTGACCGAGAATTGGATCCGAGGTCCATTACTGGTGTCGTTCTCTGTGCTCTTCAGTGTTTTGTACAACATCTTGATGGAAATGACAACCAAGAAGACAGATGCCTCGCCTCTATTTCTCACAGCATCTATATGCACTCTAGGTGCATTGATGAATTTGATTGTTGCTGTTGCAATGGAGAGTGGATCCGGATTCGTTTGGATCGTGGGCCTAAACACAAAACTCGTCTGCTATCTATATTCGGGAATTGTGGTCTCGGGTTTTACGTCATTCCTACAGAGCATGCTGCTGCGAGAGAGAGGTGCTGTGTTCTTGACGTCGTTTAGCCCCCTATCTACAATTTTTGTCATGATGGTGGGTGCTATAGTGCTTAAGGAGGTCATACATGTAGGAAGTATTCTGGGGGCTCTCATTATCGTTACAGGGCTTTTCATCCTTCAATGGGGCAAGCTCAAGGCTAGTGCATCTGAGGATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

376

Amino Acids

40.78

Weight (kDa)

8.38

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24890 FvH4_1g24890
malus_domestica MD15G1135500.v1.1
prunus_persica Prupe.4G273500_v2.0.a1
pyrus_communis pycom15g12280
rosa_chinensis RchiOBHm_Chr2g0122551
rosa_laevigata RLG00000018686
rosa_multiflora Rmu_sc0003832.1_g000004
rosa_roxburghii Rroxscaffold_2G00121480
rosa_samantha Rh2AG298100 Rh2BG306900 Rh2CG285000 Rh2DG321700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AatII GACGTC 1 cut(s) 968
AccII CGCG 1 cut(s) 331
AccIII TCCGGA 1 cut(s) 836
AciI CCGC 1 cut(s) 624
AclWI GGATC 7 cut(s) 169, 356, 658, 671, 828, 841, 857
AcoI YGGCCR 1 cut(s) 625
AcsI RAATTY 2 cut(s) 410, 802
AcuI CTGAAG 1 cut(s) 685
AcyI GRCGYC 1 cut(s) 965
AfaI GTAC 2 cut(s) 366, 713
AfiI CCNNNNNNNGG 2 cut(s) 660, 1090
AflII CTTAAG 1 cut(s) 1019
AflIII ACRYGT 1 cut(s) 1033
AgsI TTSAA 4 cut(s) 32, 74, 223, 1088
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 4 cut(s) 64, 119, 237, 1099
AluI AGCT 4 cut(s) 64, 119, 237, 1099
Alw21I GWGCWC 2 cut(s) 498, 699
Alw26I GTCTC 2 cut(s) 443, 907
AlwI GGATC 7 cut(s) 169, 356, 658, 671, 828, 841, 857
Ama87I CYCGRG 2 cut(s) 368, 904
Aor13HI TCCGGA 1 cut(s) 836
AoxI GGCC 5 cut(s) 317, 574, 586, 625, 856
ApeKI GCWGC 4 cut(s) 519, 607, 934, 937
ApoI RAATTY 2 cut(s) 410, 802
AspLEI GCGC 2 cut(s) 333, 519
AspS9I GGNCC 5 cut(s) 318, 452, 587, 671, 856
AsuHPI GGTGA 2 cut(s) 556, 562
AvaI CYCGRG 2 cut(s) 368, 904
AvaII GGWCC 2 cut(s) 452, 671
BamHI GGATCC 2 cut(s) 663, 833
BanII GRGCYC 1 cut(s) 1057
BbsI GAAGAC 1 cut(s) 749
Bbv12I GWGCWC 2 cut(s) 498, 699
BbvI GCAGC 4 cut(s) 531, 619, 921, 924
BccI CCATC 6 cut(s) 16, 541, 602, 718, 994, 1115
BciT130I CCWGG 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 443, 907
BfaI CTAG 4 cut(s) 269, 432, 788, 1107
BfmI CTRYAG 2 cut(s) 923, 1011
BfrI CTTAAG 1 cut(s) 1019
BisI GCNGC 5 cut(s) 520, 608, 625, 935, 938
BlsI GCNGC 5 cut(s) 521, 609, 626, 936, 939
Bme1390I CCNGG 1 cut(s) 478
Bme18I GGWCC 2 cut(s) 452, 671
BmeT110I CYCGRG 2 cut(s) 368, 904
BmgT120I GGNCC 5 cut(s) 318, 452, 587, 671, 856
BmiI GGNNCC 2 cut(s) 665, 835
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 3 cut(s) 739, 782, 1121
BpiI GAAGAC 1 cut(s) 749
BpmI CTGGAG 2 cut(s) 512, 579
BpuEI CTTGAG 3 cut(s) 105, 400, 1085
BsaBI GATNNNNATC 1 cut(s) 173
BsaHI GRCGYC 1 cut(s) 965
BsaI GGTCTC 1 cut(s) 907
BsaJI CCNNGG 2 cut(s) 314, 667
BsaWI WCCGGW 1 cut(s) 836
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 2 cut(s) 660, 1090
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 4 cut(s) 454, 495, 562, 684
Bse3DI GCAATG 3 cut(s) 441, 498, 828
Bse8I GATNNNNATC 1 cut(s) 173
BseAI TCCGGA 1 cut(s) 836
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 2 cut(s) 314, 667
BseGI GGATG 3 cut(s) 174, 1080, 1126
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 2 cut(s) 660, 1090
BseMI GCAATG 3 cut(s) 441, 498, 828
BseMII CTCAG 1 cut(s) 1107
BseNI ACTGG 4 cut(s) 454, 495, 562, 684
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 4 cut(s) 531, 619, 921, 924
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 5 cut(s) 319, 576, 588, 627, 858
BsiHKAI GWGCWC 2 cut(s) 498, 699
BsiHKCI CYCGRG 2 cut(s) 368, 904
BsiSI CCGG 2 cut(s) 374, 837
BslI CCNNNNNNNGG 2 cut(s) 660, 1090
BsmAI GTCTC 2 cut(s) 443, 907
BsnI GGCC 5 cut(s) 319, 576, 588, 627, 858
Bso31I GGTCTC 1 cut(s) 907
BsoBI CYCGRG 2 cut(s) 368, 904
Bsp1286I GDGCHC 3 cut(s) 498, 699, 1057
Bsp13I TCCGGA 1 cut(s) 836
Bsp1407I TGTACA 1 cut(s) 711
Bsp143I GATC 5 cut(s) 174, 348, 663, 833, 849
BspACI CCGC 1 cut(s) 624
BspANI GGCC 5 cut(s) 319, 576, 588, 627, 858
BspCNI CTCAG 1 cut(s) 1108
BspEI TCCGGA 1 cut(s) 836
BspFNI CGCG 1 cut(s) 331
BspHI TCATGA 2 cut(s) 96, 996
BspLI GGNNCC 2 cut(s) 665, 835
BspPI GGATC 7 cut(s) 169, 356, 658, 671, 828, 841, 857
BspQI GCTCTTC 1 cut(s) 704
BspTI CTTAAG 1 cut(s) 1019
BspTNI GGTCTC 1 cut(s) 907
BsrDI GCAATG 3 cut(s) 441, 498, 828
BsrFI RCCGGY 1 cut(s) 373
BsrGI TGTACA 1 cut(s) 711
BsrI ACTGG 4 cut(s) 454, 495, 562, 684
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 2 cut(s) 314, 667
BssMI GATC 5 cut(s) 174, 348, 663, 833, 849
BssNI GRCGYC 1 cut(s) 965
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 2 cut(s) 315, 601
Bst6I CTCTTC 1 cut(s) 704
BstACI GRCGYC 1 cut(s) 965
BstAFI CTTAAG 1 cut(s) 1019
BstAUI TGTACA 1 cut(s) 711
BstC8I GCNNGC 5 cut(s) 66, 84, 335, 932, 1097
BstDEI CTNAG 1 cut(s) 1116
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 3 cut(s) 174, 1080, 1126
BstFNI CGCG 1 cut(s) 331
BstHHI GCGC 2 cut(s) 333, 519
BstKTI GATC 5 cut(s) 177, 351, 666, 836, 852
BstMAI GTCTC 2 cut(s) 443, 907
BstMBI GATC 5 cut(s) 174, 348, 663, 833, 849
BstMWI GCNNNNNNNGC 1 cut(s) 613
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 2 cut(s) 934, 1037
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 2 cut(s) 923, 1011
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 4 cut(s) 531, 619, 921, 924
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 2 cut(s) 663, 833
BstYI RGATCY 2 cut(s) 663, 833
BsuRI GGCC 5 cut(s) 319, 576, 588, 627, 858
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 3 cut(s) 174, 1080, 1126
BtsIMutI CAGTG 3 cut(s) 447, 606, 709
Cac8I GCNNGC 5 cut(s) 66, 84, 335, 932, 1097
CciI TCATGA 2 cut(s) 96, 996
CfoI GCGC 2 cut(s) 333, 519
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 5 cut(s) 318, 452, 587, 671, 856
CseI GACGC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 365, 712
CviAII CATG 6 cut(s) 59, 97, 541, 931, 997, 1034
CviQI GTAC 2 cut(s) 365, 712
DdeI CTNAG 1 cut(s) 1116
DpnI GATC 5 cut(s) 176, 350, 665, 835, 851
DpnII GATC 5 cut(s) 174, 348, 663, 833, 849
EaeI YGGCCR 1 cut(s) 625
Eam1104I CTCTTC 1 cut(s) 704
EarI CTCTTC 1 cut(s) 704
Eco24I GRGCYC 1 cut(s) 1057
Eco31I GGTCTC 1 cut(s) 907
Eco47I GGWCC 2 cut(s) 452, 671
Eco57I CTGAAG 1 cut(s) 685
Eco88I CYCGRG 2 cut(s) 368, 904
EcoRII CCWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 1057
FaeI CATG 6 cut(s) 62, 100, 544, 934, 1000, 1037
FatI CATG 6 cut(s) 58, 96, 540, 930, 996, 1033
Fnu4HI GCNGC 5 cut(s) 520, 608, 625, 935, 938
FokI GGATG 2 cut(s) 181, 1067
FriOI GRGCYC 1 cut(s) 1057
Fsp4HI GCNGC 5 cut(s) 520, 608, 625, 935, 938
FspBI CTAG 4 cut(s) 269, 432, 788, 1107
GlaI GCGC 2 cut(s) 332, 518
GluI GCNGC 5 cut(s) 520, 608, 625, 935, 938
GsuI CTGGAG 2 cut(s) 512, 579
HaeIII GGCC 5 cut(s) 319, 576, 588, 627, 858
HapII CCGG 2 cut(s) 374, 837
HgaI GACGC 1 cut(s) 337
HhaI GCGC 2 cut(s) 333, 519
Hin1I GRCGYC 1 cut(s) 965
Hin1II CATG 6 cut(s) 62, 100, 544, 934, 1000, 1037
Hin6I GCGC 2 cut(s) 331, 517
HinP1I GCGC 2 cut(s) 331, 517
HincII GTYRAC 1 cut(s) 11
HindII GTYRAC 1 cut(s) 11
HinfI GANTC 1 cut(s) 840
HpaII CCGG 2 cut(s) 374, 837
HphI GGTGA 2 cut(s) 556, 562
Hpy166II GTNNAC 1 cut(s) 11
Hpy188I TCNGA 3 cut(s) 348, 668, 1117
Hpy188III TCNNGA 8 cut(s) 97, 379, 402, 721, 837, 891, 961, 997
Hpy8I GTNNAC 1 cut(s) 11
Hpy99I CGWCG 1 cut(s) 970
HpyAV CCTTC 2 cut(s) 217, 1094
HpyCH4III ACNGT 2 cut(s) 315, 601
HpyCH4IV ACGT 2 cut(s) 914, 965
HpyCH4V TGCA 6 cut(s) 503, 607, 783, 794, 821, 1112
HpyF10VI GCNNNNNNNGC 1 cut(s) 613
HpyF3I CTNAG 1 cut(s) 1116
HpySE526I ACGT 2 cut(s) 914, 965
Hsp92I GRCGYC 1 cut(s) 965
Hsp92II CATG 6 cut(s) 62, 100, 544, 934, 1000, 1037
HspAI GCGC 2 cut(s) 331, 517
Kpn2I TCCGGA 1 cut(s) 836
Kzo9I GATC 5 cut(s) 174, 348, 663, 833, 849
LguI GCTCTTC 1 cut(s) 704
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 4 cut(s) 531, 619, 921, 924
LweI GCATC 3 cut(s) 739, 782, 1121
MaeI CTAG 4 cut(s) 269, 432, 788, 1107
MaeII ACGT 2 cut(s) 914, 965
MaeIII GTNAC 2 cut(s) 282, 1066
MalI GATC 5 cut(s) 176, 350, 665, 835, 851
MboI GATC 5 cut(s) 174, 348, 663, 833, 849
MboII GAAGA 6 cut(s) 44, 122, 125, 196, 691, 754
MflI RGATCY 2 cut(s) 663, 833
MhlI GDGCHC 3 cut(s) 498, 699, 1057
MluCI AATT 9 cut(s) 36, 186, 410, 504, 530, 658, 802, 894, 987
MroI TCCGGA 1 cut(s) 836
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 6 cut(s) 167, 545, 599, 722, 778, 1001
MspA1I CMGCKG 1 cut(s) 624
MspCI CTTAAG 1 cut(s) 1019
MspI CCGG 2 cut(s) 374, 837
MspR9I CCNGG 1 cut(s) 478
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 1 cut(s) 613
NdeII GATC 5 cut(s) 174, 348, 663, 833, 849
NlaIII CATG 6 cut(s) 62, 100, 544, 934, 1000, 1037
NlaIV GGNNCC 2 cut(s) 665, 835
NspI RCATGY 2 cut(s) 934, 1037
PaeI GCATGC 1 cut(s) 934
PaeR7I CTCGAG 1 cut(s) 368
PagI TCATGA 2 cut(s) 96, 996
PciI ACATGT 1 cut(s) 1033
PciSI GCTCTTC 1 cut(s) 704
PcsI WCGNNNNNNNCGW 2 cut(s) 849, 911
PfeI GAWTC 1 cut(s) 840
PfoI TCCNGGA 1 cut(s) 476
PkrI GCNGC 5 cut(s) 521, 609, 626, 936, 939
PscI ACATGT 1 cut(s) 1033
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 476
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 2 cut(s) 665, 835
PspPI GGNCC 5 cut(s) 318, 452, 587, 671, 856
PspXI VCTCGAGB 1 cut(s) 368
PsuI RGATCY 2 cut(s) 663, 833
RsaI GTAC 2 cut(s) 366, 713
RsaNI GTAC 2 cut(s) 365, 712
RseI CAYNNNNRTG 6 cut(s) 167, 545, 599, 722, 778, 1001
SapI GCTCTTC 1 cut(s) 704
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 5 cut(s) 520, 608, 625, 935, 938
Sau3AI GATC 5 cut(s) 174, 348, 663, 833, 849
Sau96I GGNCC 5 cut(s) 318, 452, 587, 671, 856
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 3 cut(s) 498, 699, 1057
SfaNI GCATC 3 cut(s) 739, 782, 1121
SfcI CTRYAG 2 cut(s) 923, 1011
Sfr274I CTCGAG 1 cut(s) 368
SinI GGWCC 2 cut(s) 452, 671
SlaI CTCGAG 1 cut(s) 368
SmiMI CAYNNNNRTG 6 cut(s) 167, 545, 599, 722, 778, 1001
SmlI CTYRAG 5 cut(s) 120, 368, 379, 1019, 1100
SmoI CTYRAG 5 cut(s) 120, 368, 379, 1019, 1100
SphI GCATGC 1 cut(s) 934
Sse9I AATT 9 cut(s) 36, 186, 410, 504, 530, 658, 802, 894, 987
SsiI CCGC 1 cut(s) 624
SspMI CTAG 4 cut(s) 269, 432, 788, 1107
StyD4I CCNGG 1 cut(s) 476
TaaI ACNGT 2 cut(s) 315, 601
TaiI ACGT 2 cut(s) 917, 968
TaqI TCGA 2 cut(s) 104, 369
TaqII GACCGA 1 cut(s) 668
TasI AATT 9 cut(s) 36, 186, 410, 504, 530, 658, 802, 894, 987
TatI WGTACW 1 cut(s) 711
TauI GCSGC 1 cut(s) 627
TfiI GAWTC 1 cut(s) 840
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 3 cut(s) 454, 606, 709
TseI GCWGC 4 cut(s) 519, 607, 934, 937
TspDTI ATGAA 3 cut(s) 113, 815, 1069
TspRI CASTG 3 cut(s) 454, 606, 709
Vha464I CTTAAG 1 cut(s) 1019
VpaK11BI GGWCC 2 cut(s) 452, 671
XapI RAATTY 2 cut(s) 410, 802
XceI RCATGY 2 cut(s) 934, 1037
XhoI CTCGAG 1 cut(s) 368
XspI CTAG 4 cut(s) 269, 432, 788, 1107
ZraI GACGTC 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.