RLG00000018686

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017232
Physical Location & Seq
Reverse (-)
33941691 .. 33944266
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018686

Sequence Viewer

Length: 1914 bp
ATGGCTGAGATTTGGGGCGCTATAGTAGGAGTTTCCTCTTGGATCATTGGACCGGTGATTCCATTGATTCTAGGGGGAAATTCTATTATATGCAGGAGGGCTTTTGAGAAGGGGGTCACAATTGGTGTGTTTCTATTTTTTAAGAACTTCCTGGCCACTGTTGTATTAGCATCTGCAGCCGTCATTTCTAGGGCCGATAGGACTCCACTGGGGTGTGCAGGAGTCTTTCGAGTTCTTCATCTAGCTATTCTTGATGTTGTGAGTCAGTTGTGTTCCTTTACTGGTCTTCGATACACATCGGCCAGTTTTTCTTCATCGTTGGCTGCCTGTGGTCCAGCATTCATTTTTTTAGCAGCATGCTTTTGTGGGATGGAGTATTTCCAGTGGACTGCCAGGAGTTTTCAGGCTAAAGTCTTTGGTATGACACTGATGGTGGGCGGAGGCTTTATCGTGGGATTTTATCAAGGCCCCACCATCAGTATCATGCACTCTCCTGCTAGTAGCCATATTGGGGTGGATGCACGAGAGCTTAGAGAGGACTGGTTTCGTGGTCCACTTCTAATTTTAGTTGCATTAGCTGCCTCAGTTTGGTATAATCTTCAGATTAAGAATGTGGCGGCGCAGTATGGATCAGCCATATGGCTGAGTTTCTACATAACTGCTGCGGATACACTTATGACTTTGGCAGTTGCTCTGGGGTTAGAGGTGAGATCTCCATTTGCATGGAAGATTATAGCAGACATCAGACTGGTCAGCTACGTATATTCGGGTGTCGTTGTTTCCGGTCTTGCTTCATTCCTCCAGATCACACTCACTAGGATGAGAGATCCAGTCTTCGCAGCTGCATTTAGCCCGATATCACTAGTCTTTGTTATGATGATGAGTTTGGTAGCATTGAGGGACGTTATACATCTTGGAAGCATTATGGGGGCGATTTCCATCATAGTTGGGCTTTTCATGCTTCTTTGGGGAAAGATGCATGATCCTACACCCCCAGTACTCCCTATTCAGGAGTCGGCATCCCTACCTGGACCACACTCATCACCTGCATCAGCCGAAGCGAGGACAGTAGGAGAGGCAGATGAGTTATCTATACTAGAGCGGGGAGAGCAGGAGCGACGACAGCATCCAGAGGCATGTCAGTCACAGGAAAGGCCAGAGACACAGCAGGCAGAGCAAGAGACAGAGTTGCTAGAGCGACTGAGAGAGGCAGAGGTGAGAGAGCAGCCAGGGCTAGAGCCAGCTGAGTCTAGACAAGAGAGGTCAGAGACACAGTTAGAAAGGCCGAGAGAGGCAGAGCTGAGAGAGATAGAGATGCCTGCACCAGAGCAGCCAGCGGTAGACACTTCTACACAGGAAATGATGGAGGTAGAGGAGTTAGAGCAGCCAGGGCTGCCCGCACTAGAGCAGCCAGAGGCTGACGGGTCCTCGAAAACACAGTTAGTGCGGCCGAGAGAGGCAGAACTTAGAGAGCGAGAGCAGCCAGTGGTACACACTTCTACACAGGAGAGGATAGATGTAGAGGAGTCAGAGCAACCAGGGCTACCCGCACTAGAGCAGCTAGAGGCTGACGAGTCTTTAGAGACACAGTTAGAGCGGCCGAGAGAGGCAGAGCTTAGAGAGCCAGAGCAGCCAGCGATAGACACTTCTACACAGGAGAGGATAGATGTAGAGGAGTCAGAGCAGCCAGGGCTACCCGCACTAGAGCAGCTAGAGGCTGACGAGTCTTCGGAGACACAGTTAGAGCGGCCGAGAGAGGCAGAGCAGCCAACTGTAGACACTTCTACACAGGAGAGGATGGAGGTAGAGGAGTTAGAGCAGCCAGAGGCTGACGAGTCCTCGGAGACACAGTTACAGCAGCTGAGAGAGGCAGAGCTTAGAGAGCCAGAGCTGCCCGCACATGATTCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

638

Amino Acids

70.44

Weight (kDa)

4.51

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24890 FvH4_1g24890
malus_domestica MD15G1135500.v1.1
prunus_persica Prupe.4G273500_v2.0.a1
pyrus_communis pycom15g12280
rosa_chinensis RchiOBHm_Chr2g0122551
rosa_laevigata RLG00000018686
rosa_multiflora Rmu_sc0003832.1_g000004
rosa_roxburghii Rroxscaffold_2G00121480
rosa_samantha Rh2AG298100 Rh2BG306900 Rh2CG285000 Rh2DG321700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1054
Acc36I ACCTGC 1 cut(s) 1054
AccBSI CCGCTC 3 cut(s) 1102, 1597, 1747
AccI GTMKAC 2 cut(s) 1341, 1776
AclWI GGATC 4 cut(s) 50, 637, 821, 977
AcoI YGGCCR 5 cut(s) 153, 300, 1448, 1598, 1748
AcsI RAATTY 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 2 cut(s) 999, 1491
AfiI CCNNNNNNNGG 4 cut(s) 511, 588, 1009, 1062
AgeI ACCGGT 1 cut(s) 52
AhlI ACTAGT 1 cut(s) 862
AjnI CCWGG 7 cut(s) 150, 392, 1027, 1228, 1387, 1537, 1687
AleI CACNNNNGTG 1 cut(s) 211
Alw26I GTCTC 6 cut(s) 1154, 1175, 1262, 1577, 1727, 1838
AlwI GGATC 4 cut(s) 50, 637, 821, 977
AlwNI CAGNNNCTG 3 cut(s) 1418, 1829, 1861
AoxI GGCC 9 cut(s) 153, 192, 300, 466, 1154, 1283, 1448, 1598, 1748
ApoI RAATTY 1 cut(s) 79
AsiGI ACCGGT 1 cut(s) 52
AspLEI GCGC 2 cut(s) 20, 622
AspS9I GGNCC 7 cut(s) 50, 192, 332, 467, 551, 1031, 1425
AsuHPI GGTGA 4 cut(s) 67, 718, 1035, 1228
AvaII GGWCC 5 cut(s) 50, 332, 551, 1031, 1425
BalI TGGCCA 1 cut(s) 155
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 3 cut(s) 278, 826, 1719
BccI CCATC 6 cut(s) 364, 424, 482, 947, 1357, 1792
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
BciVI GTATCC 1 cut(s) 661
BcoDI GTCTC 6 cut(s) 1154, 1175, 1262, 1577, 1727, 1838
BcuI ACTAGT 1 cut(s) 862
BfmI CTRYAG 3 cut(s) 21, 174, 1773
BfoI RGCGCY 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 1054
BfuI GTATCC 1 cut(s) 661
BglII AGATCT 1 cut(s) 710
BmcAI AGTACT 1 cut(s) 999
Bme1390I CCNGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
Bme18I GGWCC 5 cut(s) 50, 332, 551, 1031, 1425
BmgT120I GGNCC 7 cut(s) 50, 192, 332, 467, 551, 1031, 1425
BmiI GGNNCC 2 cut(s) 469, 1426
BmrFI CCNGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
BmrI ACTGGG 2 cut(s) 218, 989
BmsI GCATC 7 cut(s) 179, 508, 966, 1028, 1058, 1135, 1305
BmuI ACTGGG 2 cut(s) 218, 989
BpiI GAAGAC 3 cut(s) 278, 826, 1719
BpmI CTGGAG 1 cut(s) 785
BsaAI YACGTR 1 cut(s) 760
BsaBI GATNNNNATC 2 cut(s) 295, 938
BsaJI CCNNGG 5 cut(s) 1229, 1388, 1538, 1688, 1839
BsaWI WCCGGW 2 cut(s) 52, 782
BsaXI ACNNNNNCTCC 4 cut(s) 1724, 1754, 1835, 1865
Bsc4I CCNNNNNNNGG 4 cut(s) 511, 588, 1009, 1062
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 9 cut(s) 213, 286, 303, 382, 545, 753, 830, 995, 1484
Bse8I GATNNNNATC 2 cut(s) 295, 938
BseBI CCWGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
BseDI CCNNGG 5 cut(s) 1229, 1388, 1538, 1688, 1839
BseGI GGATG 6 cut(s) 375, 523, 825, 1019, 1126, 1803
BseJI GATNNNNATC 2 cut(s) 295, 938
BseLI CCNNNNNNNGG 4 cut(s) 511, 588, 1009, 1062
BseMII CTCAG 6 cut(s) 597, 635, 1193, 1236, 1292, 1853
BseNI ACTGG 9 cut(s) 213, 286, 303, 382, 545, 753, 830, 995, 1484
BseRI GAGGAG 4 cut(s) 1388, 1538, 1688, 1823
BseX3I CGGCCG 3 cut(s) 1448, 1598, 1748
BsgI GTGCAG 2 cut(s) 237, 1305
Bsh1285I CGRYCG 3 cut(s) 1451, 1601, 1751
BshFI GGCC 9 cut(s) 155, 194, 302, 468, 1156, 1285, 1450, 1600, 1750
BshTI ACCGGT 1 cut(s) 52
BsiEI CGRYCG 3 cut(s) 1451, 1601, 1751
BsiSI CCGG 2 cut(s) 53, 783
BslFI GGGAC 1 cut(s) 914
BslI CCNNNNNNNGG 4 cut(s) 511, 588, 1009, 1062
BsmAI GTCTC 6 cut(s) 1154, 1175, 1262, 1577, 1727, 1838
BsmFI GGGAC 1 cut(s) 914
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 9 cut(s) 155, 194, 302, 468, 1156, 1285, 1450, 1600, 1750
Bsp143I GATC 6 cut(s) 42, 629, 710, 804, 826, 982
BspANI GGCC 9 cut(s) 155, 194, 302, 468, 1156, 1285, 1450, 1600, 1750
BspCNI CTCAG 6 cut(s) 596, 636, 1194, 1237, 1293, 1854
BspLI GGNNCC 2 cut(s) 469, 1426
BspMAI CTGCAG 1 cut(s) 178
BspMI ACCTGC 1 cut(s) 1054
BspPI GGATC 4 cut(s) 50, 637, 821, 977
BsrBI CCGCTC 3 cut(s) 1102, 1597, 1747
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 9 cut(s) 213, 286, 303, 382, 545, 753, 830, 995, 1484
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 5 cut(s) 1229, 1388, 1538, 1688, 1839
BssMI GATC 6 cut(s) 42, 629, 710, 804, 826, 982
BssSI CACGAG 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
Bst4CI ACNGT 8 cut(s) 160, 1069, 1275, 1440, 1590, 1740, 1774, 1851
BstAPI GCANNNNNTGC 1 cut(s) 578
BstBAI YACGTR 1 cut(s) 760
BstC8I GCNNGC 8 cut(s) 358, 1170, 1242, 1320, 1335, 1398, 1635, 1896
BstF5I GGATG 6 cut(s) 375, 523, 825, 1019, 1126, 1803
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 2 cut(s) 20, 622
BstKTI GATC 6 cut(s) 45, 632, 713, 807, 829, 985
BstMAI GTCTC 6 cut(s) 1154, 1175, 1262, 1577, 1727, 1838
BstMBI GATC 6 cut(s) 42, 629, 710, 804, 826, 982
BstMCI CGRYCG 3 cut(s) 1451, 1601, 1751
BstNI CCWGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
BstNSI RCATGY 2 cut(s) 360, 1140
BstSCI CCNGG 7 cut(s) 150, 392, 1027, 1228, 1387, 1537, 1687
BstSFI CTRYAG 3 cut(s) 21, 174, 1773
BstSNI TACGTA 1 cut(s) 760
BstV2I GAAGAC 3 cut(s) 278, 826, 1719
BstX2I RGATCY 2 cut(s) 710, 826
BstXI CCANNNNNNTGG 1 cut(s) 723
BstYI RGATCY 2 cut(s) 710, 826
BstZI CGGCCG 3 cut(s) 1448, 1598, 1748
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 9 cut(s) 155, 194, 302, 468, 1156, 1285, 1450, 1600, 1750
BtsCI GGATG 6 cut(s) 375, 523, 825, 1019, 1126, 1803
BtsIMutI CAGTG 5 cut(s) 156, 206, 389, 425, 1491
BveI ACCTGC 1 cut(s) 1054
Cac8I GCNNGC 8 cut(s) 358, 1170, 1242, 1320, 1335, 1398, 1635, 1896
CaiI CAGNNNCTG 3 cut(s) 1418, 1829, 1861
CfoI GCGC 2 cut(s) 20, 622
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 7 cut(s) 50, 192, 332, 467, 551, 1031, 1425
Csp6I GTAC 2 cut(s) 998, 1490
CspAI ACCGGT 1 cut(s) 52
CviAII CATG 7 cut(s) 357, 484, 723, 958, 980, 1137, 1901
CviQI GTAC 2 cut(s) 998, 1490
DpnI GATC 6 cut(s) 44, 631, 712, 806, 828, 984
DpnII GATC 6 cut(s) 42, 629, 710, 804, 826, 982
EaeI YGGCCR 5 cut(s) 153, 300, 1448, 1598, 1748
EagI CGGCCG 3 cut(s) 1448, 1598, 1748
EciI GGCGGA 1 cut(s) 453
EclXI CGGCCG 3 cut(s) 1448, 1598, 1748
Eco105I TACGTA 1 cut(s) 760
Eco32I GATATC 1 cut(s) 858
Eco47I GGWCC 5 cut(s) 50, 332, 551, 1031, 1425
Eco52I CGGCCG 3 cut(s) 1448, 1598, 1748
Eco57I CTGAAG 1 cut(s) 584
EcoO109I RGGNCCY 2 cut(s) 467, 1425
EcoRII CCWGG 7 cut(s) 150, 392, 1027, 1228, 1387, 1537, 1687
EcoRV GATATC 1 cut(s) 858
EcoT22I ATGCAT 1 cut(s) 981
FaeI CATG 7 cut(s) 360, 487, 726, 961, 983, 1140, 1904
FaqI GGGAC 1 cut(s) 914
FatI CATG 7 cut(s) 356, 483, 722, 957, 979, 1136, 1900
FauI CCCGC 5 cut(s) 1095, 1405, 1555, 1705, 1903
FauNDI CATATG 1 cut(s) 638
FblI GTMKAC 2 cut(s) 1341, 1776
FokI GGATG 6 cut(s) 382, 530, 832, 1006, 1113, 1810
GlaI GCGC 2 cut(s) 19, 621
GsuI CTGGAG 1 cut(s) 785
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 9 cut(s) 155, 194, 302, 468, 1156, 1285, 1450, 1600, 1750
HapII CCGG 2 cut(s) 53, 783
HhaI GCGC 2 cut(s) 20, 622
Hin1II CATG 7 cut(s) 360, 487, 726, 961, 983, 1140, 1904
Hin6I GCGC 2 cut(s) 18, 620
HinP1I GCGC 2 cut(s) 18, 620
HpaII CCGG 2 cut(s) 53, 783
HphI GGTGA 4 cut(s) 67, 718, 1035, 1228
Hpy166II GTNNAC 5 cut(s) 387, 554, 1342, 1492, 1777
Hpy188I TCNGA 7 cut(s) 603, 746, 1267, 1531, 1681, 1732, 1843
Hpy188III TCNNGA 5 cut(s) 251, 802, 1010, 1130, 1251
Hpy8I GTNNAC 5 cut(s) 387, 554, 1342, 1492, 1777
Hpy99I CGWCG 1 cut(s) 1122
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 8 cut(s) 160, 1069, 1275, 1440, 1590, 1740, 1774, 1851
HpyCH4IV ACGT 2 cut(s) 759, 903
HpySE526I ACGT 2 cut(s) 759, 903
Hsp92II CATG 7 cut(s) 360, 487, 726, 961, 983, 1140, 1904
HspAI GCGC 2 cut(s) 18, 620
Kzo9I GATC 6 cut(s) 42, 629, 710, 804, 826, 982
LmnI GCTCC 1 cut(s) 1114
LweI GCATC 7 cut(s) 179, 508, 966, 1028, 1058, 1135, 1305
MaeII ACGT 2 cut(s) 759, 903
MaeIII GTNAC 3 cut(s) 115, 1143, 1851
MalI GATC 6 cut(s) 44, 631, 712, 806, 828, 984
MbiI CCGCTC 3 cut(s) 1102, 1597, 1747
MboI GATC 6 cut(s) 42, 629, 710, 804, 826, 982
MboII GAAGA 7 cut(s) 227, 278, 303, 590, 739, 826, 1719
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 2 cut(s) 710, 826
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 3 cut(s) 79, 120, 561
MluNI TGGCCA 1 cut(s) 155
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 981
MscI TGGCCA 1 cut(s) 155
MseI TTAA 2 cut(s) 141, 606
MslI CAYNNNNRTG 3 cut(s) 211, 721, 818
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 4 cut(s) 842, 1244, 1337, 1861
MspI CCGG 2 cut(s) 53, 783
MspR9I CCNGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
NdeI CATATG 1 cut(s) 638
NdeII GATC 6 cut(s) 42, 629, 710, 804, 826, 982
NlaIII CATG 7 cut(s) 360, 487, 726, 961, 983, 1140, 1904
NlaIV GGNNCC 2 cut(s) 469, 1426
NmeAIII GCCGAG 4 cut(s) 1311, 1476, 1626, 1776
NmuCI GTSAC 2 cut(s) 115, 1143
NsiI ATGCAT 1 cut(s) 981
NspI RCATGY 2 cut(s) 360, 1140
OliI CACNNNNGTG 1 cut(s) 211
PaeI GCATGC 1 cut(s) 360
PaqCI CACCTGC 1 cut(s) 1054
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 3 cut(s) 58, 67, 1904
PinAI ACCGGT 1 cut(s) 52
Ppu21I YACGTR 1 cut(s) 760
PpuMI RGGWCCY 1 cut(s) 1425
Psp5II RGGWCCY 1 cut(s) 1425
Psp6I CCWGG 7 cut(s) 150, 392, 1027, 1228, 1387, 1537, 1687
PspGI CCWGG 7 cut(s) 150, 392, 1027, 1228, 1387, 1537, 1687
PspN4I GGNNCC 2 cut(s) 469, 1426
PspPI GGNCC 7 cut(s) 50, 192, 332, 467, 551, 1031, 1425
PspPPI RGGWCCY 1 cut(s) 1425
PstI CTGCAG 1 cut(s) 178
PstNI CAGNNNCTG 3 cut(s) 1418, 1829, 1861
PsuI RGATCY 2 cut(s) 710, 826
PvuII CAGCTG 3 cut(s) 842, 1244, 1861
RsaI GTAC 2 cut(s) 999, 1491
RsaNI GTAC 2 cut(s) 998, 1490
RseI CAYNNNNRTG 3 cut(s) 211, 721, 818
SaqAI TTAA 2 cut(s) 141, 606
Sau3AI GATC 6 cut(s) 42, 629, 710, 804, 826, 982
Sau96I GGNCC 7 cut(s) 50, 192, 332, 467, 551, 1031, 1425
ScaI AGTACT 1 cut(s) 999
ScrFI CCNGG 7 cut(s) 152, 394, 1029, 1230, 1389, 1539, 1689
SfaNI GCATC 7 cut(s) 179, 508, 966, 1028, 1058, 1135, 1305
SfcI CTRYAG 3 cut(s) 21, 174, 1773
SinI GGWCC 5 cut(s) 50, 332, 551, 1031, 1425
SmiMI CAYNNNNRTG 3 cut(s) 211, 721, 818
SnaBI TACGTA 1 cut(s) 760
SpeI ACTAGT 1 cut(s) 862
SphI GCATGC 1 cut(s) 360
Sse9I AATT 3 cut(s) 79, 120, 561
StyD4I CCNGG 7 cut(s) 150, 392, 1027, 1228, 1387, 1537, 1687
TaaI ACNGT 8 cut(s) 160, 1069, 1275, 1440, 1590, 1740, 1774, 1851
TaiI ACGT 2 cut(s) 762, 906
TaqI TCGA 3 cut(s) 229, 289, 1430
TasI AATT 3 cut(s) 79, 120, 561
TatI WGTACW 1 cut(s) 997
TauI GCSGC 4 cut(s) 620, 1450, 1600, 1750
TfiI GAWTC 3 cut(s) 58, 67, 1904
Tru1I TTAA 2 cut(s) 141, 606
Tru9I TTAA 2 cut(s) 141, 606
TscAI CASTG 5 cut(s) 163, 213, 389, 432, 1491
TseFI GTSAC 2 cut(s) 115, 1143
Tsp45I GTSAC 2 cut(s) 115, 1143
TspDTI ATGAA 5 cut(s) 227, 303, 331, 783, 946
TspRI CASTG 5 cut(s) 163, 213, 389, 432, 1491
VpaK11BI GGWCC 5 cut(s) 50, 332, 551, 1031, 1425
XapI RAATTY 1 cut(s) 79
XbaI TCTAGA 1 cut(s) 1250
XceI RCATGY 2 cut(s) 360, 1140
XmiI GTMKAC 2 cut(s) 1341, 1776
ZrmI AGTACT 1 cut(s) 999
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.