Rmu_sc0003832.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003832.1
OG0017232
Physical Location & Seq
Forward (+)
20881 .. 26773
5893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003832.1_g000004.1.cds

Sequence Viewer

Length: 1665 bp
atggagtatgtccagtggactgcgaggagttttcaggctaaagttttaggtatgatactgatggtgggcggaggctgtatcgtgggattttatcagggcctcaccatcagtatcatgcactctcctgttggtagccacagtgggatgaatacacgagagcttagagaggactggtttcgtagtccacttctagttttagttgcattagctgcctcggtttgggtgtcgttgtttcgggtcttgcttcattcctccaaattaatcacacttgctaggatgagagatcccgtcttcgtagccgtatttagcccaatctcactagtctttgttatggtgatgagtttggatgttaaaggtgagatctccatttgcatggaagattatagtagacatcagattggtcggctatatatattcgggctggctgcggcacggttgccgacatttttggggtcagccgaataccgaccgaaaatcttcggtttcgtcagaattgaaaccggtaccgtgccggaagttcggttaccgaaacctcggtttaccgaggtaaacagtcggtatcggttggtattttggcacaccaatcgtcggaggtcggtgaggtccgtgatggtggaggtgagtcacggtggtgaccggaaatgggtgtcggaggagagagaaaatagatctaatccgccactggatgaatgggtacctcgaatcgaagctggaggcgacgaaatccccaattacgtacaccctgactattttacaatcaaggtataccatggtggctacatagagcatgaggatagcaagtacattggtgcaactgtcagctactatgataatgtggacaaggataaaatgtcattgactgaggtggatggcatggtgagatggatcaagtctgagtatgtgggaacaaggattgattactggtactctattggcagtgaggaagatgccatgtctaagctgaacaatgactcgaatgtcatcagaatgtgttgcatggagagtcaggagagtaaggattcatgggattggtttttgaggttgttggtgaaagatttggagattacaagagatggcagtggttggactttcatatcagacaaacagaaaggactgcttcctgcatgtgaagttgtggtgcctcttgcagaacataggttctgtgttagacacttgtcgactaatttcaataagctgtttcctgggaaagttttcaaggatcaggtctgggcaattgccaaagcaacaaccatggcctactacaccaaagagctggttttgatgaagcaaatggaccccaaagcttatgactggttaacagatcctttgaggccccaaaagcattggtctaaagctcactttggcaccaccctgaaatgtgatgttcttcttaataatttatgtgaaagtttcaatgcttttattttacctgctagaagcaagcctgtgatttcatgctttgaaggtattatggtcaagatgatgaagaggattgcaatgagaagagacaagattagcaaagttgtggaccctatatgtcccaagcctagagaactcctaaagaagaacaagattaaatctgcaactgattgcataccatatggctctagcagcccccagattgaggtagaaagtttggaggatccaagcatgtggttgacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

554

Amino Acids

63.64

Weight (kDa)

8.83

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24890 FvH4_1g24890
malus_domestica MD15G1135500.v1.1
prunus_persica Prupe.4G273500_v2.0.a1
pyrus_communis pycom15g12280
rosa_chinensis RchiOBHm_Chr2g0122551
rosa_laevigata RLG00000018686
rosa_multiflora Rmu_sc0003832.1_g000004
rosa_roxburghii Rroxscaffold_2G00121480
rosa_samantha Rh2AG298100 Rh2BG306900 Rh2CG285000 Rh2DG321700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 977
Acc36I ACCTGC 1 cut(s) 1438
Acc65I GGTACC 2 cut(s) 503, 694
AccB1I GGYRCC 4 cut(s) 503, 694, 1138, 1364
AccI GTMKAC 3 cut(s) 388, 765, 1178
AciI CCGC 3 cut(s) 69, 428, 677
AclWI GGATC 6 cut(s) 278, 893, 1227, 1316, 1637, 1650
AfaI GTAC 5 cut(s) 505, 696, 738, 803, 926
AfiI CCNNNNNNNGG 4 cut(s) 219, 588, 643, 1624
AgeI ACCGGT 1 cut(s) 500
AgsI TTSAA 5 cut(s) 497, 1189, 1216, 1414, 1463
AhlI ACTAGT 1 cut(s) 319
AjnI CCWGG 1 cut(s) 1201
AleI CACNNNNGTG 1 cut(s) 630
AluBI AGCT 9 cut(s) 160, 209, 710, 822, 961, 1195, 1273, 1304, 1355
AluI AGCT 9 cut(s) 160, 209, 710, 822, 961, 1195, 1273, 1304, 1355
Alw26I GTCTC 1 cut(s) 1500
AlwI GGATC 6 cut(s) 278, 893, 1227, 1316, 1637, 1650
AoxI GGCC 3 cut(s) 97, 1254, 1331
ApeKI GCWGC 3 cut(s) 209, 425, 1611
AseI ATTAAT 1 cut(s) 260
AsiGI ACCGGT 1 cut(s) 500
Asp700I GAANNNNTTC 2 cut(s) 476, 1211
Asp718I GGTACC 2 cut(s) 503, 694
AspS9I GGNCC 5 cut(s) 97, 603, 1294, 1332, 1528
AsuHPI GGTGA 8 cut(s) 94, 346, 368, 610, 631, 644, 889, 1060
AvaII GGWCC 3 cut(s) 603, 1294, 1528
BamHI GGATCC 1 cut(s) 1642
BanI GGYRCC 4 cut(s) 503, 694, 1138, 1364
BarI GAAGNNNNNNTAC 2 cut(s) 507, 539
BauI CACGAG 1 cut(s) 153
BbsI GAAGAC 1 cut(s) 283
BbvI GCAGC 3 cut(s) 196, 412, 1623
BccI CCATC 6 cut(s) 55, 113, 604, 863, 876, 1067
BceAI ACGGC 1 cut(s) 284
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 1 cut(s) 1203
BcoDI GTCTC 1 cut(s) 1500
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 6 cut(s) 191, 273, 320, 1434, 1548, 1608
BfuAI ACCTGC 1 cut(s) 1438
BglII AGATCT 2 cut(s) 360, 668
BisI GCNGC 4 cut(s) 210, 426, 429, 1612
BlsI GCNGC 4 cut(s) 211, 427, 430, 1613
Bme1390I CCNGG 1 cut(s) 1203
Bme18I GGWCC 3 cut(s) 603, 1294, 1528
BmgT120I GGNCC 5 cut(s) 97, 603, 1294, 1332, 1528
BmiI GGNNCC 8 cut(s) 505, 696, 1140, 1296, 1334, 1366, 1530, 1644
BmrFI CCNGG 1 cut(s) 1203
BmsI GCATC 1 cut(s) 937
BoxI GACNNNNGTC 1 cut(s) 1174
BpiI GAAGAC 1 cut(s) 283
BpmI CTGGAG 1 cut(s) 732
BsaAI YACGTR 1 cut(s) 736
BsaJI CCNNGG 6 cut(s) 213, 533, 543, 769, 1202, 1251
BsaWI WCCGGW 2 cut(s) 500, 636
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 588, 643, 1624
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 5 cut(s) 13, 176, 687, 926, 1316
Bse3DI GCAATG 1 cut(s) 1503
BseBI CCWGG 1 cut(s) 1203
BseDI CCNNGG 6 cut(s) 213, 533, 543, 769, 1202, 1251
BseGI GGATG 5 cut(s) 150, 282, 352, 691, 874
BseLI CCNNNNNNNGG 4 cut(s) 219, 588, 643, 1624
BseMI GCAATG 1 cut(s) 1503
BseMII CTCAG 2 cut(s) 852, 885
BseNI ACTGG 5 cut(s) 13, 176, 687, 926, 1316
BseRI GAGGAG 2 cut(s) 40, 668
BseXI GCAGC 3 cut(s) 196, 412, 1623
Bsh1285I CGRYCG 1 cut(s) 470
BshFI GGCC 3 cut(s) 99, 1256, 1333
BshNI GGYRCC 4 cut(s) 503, 694, 1138, 1364
BshTI ACCGGT 1 cut(s) 500
BsiEI CGRYCG 1 cut(s) 470
BsiSI CCGG 3 cut(s) 501, 512, 637
BslFI GGGAC 1 cut(s) 1524
BslI CCNNNNNNNGG 4 cut(s) 219, 588, 643, 1624
BsmAI GTCTC 1 cut(s) 1500
BsmFI GGGAC 1 cut(s) 1524
BsnI GGCC 3 cut(s) 99, 1256, 1333
Bsp143I GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
Bsp19I CCATGG 2 cut(s) 769, 1251
BspACI CCGC 3 cut(s) 69, 428, 677
BspANI GGCC 3 cut(s) 99, 1256, 1333
BspCNI CTCAG 2 cut(s) 853, 886
BspLI GGNNCC 8 cut(s) 505, 696, 1140, 1296, 1334, 1366, 1530, 1644
BspMI ACCTGC 1 cut(s) 1438
BspPI GGATC 6 cut(s) 278, 893, 1227, 1316, 1637, 1650
BspT107I GGYRCC 4 cut(s) 503, 694, 1138, 1364
BsrDI GCAATG 1 cut(s) 1503
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 5 cut(s) 13, 176, 687, 926, 1316
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 6 cut(s) 213, 533, 543, 769, 1202, 1251
BssMI GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
BssNAI GTATAC 1 cut(s) 766
BssSI CACGAG 1 cut(s) 153
BssT1I CCWWGG 2 cut(s) 769, 1251
Bst1107I GTATAC 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 1203
Bst4CI ACNGT 6 cut(s) 140, 435, 508, 554, 629, 817
Bst6I CTCTTC 2 cut(s) 1481, 1498
BstAPI GCANNNNNTGC 1 cut(s) 209
BstBAI YACGTR 1 cut(s) 736
BstC8I GCNNGC 2 cut(s) 423, 1442
BstDEI CTNAG 4 cut(s) 161, 861, 894, 957
BstDSI CCRYGG 2 cut(s) 769, 1251
BstEII GGTNACC 2 cut(s) 522, 632
BstF5I GGATG 5 cut(s) 150, 282, 352, 691, 874
BstKTI GATC 7 cut(s) 286, 363, 671, 888, 1222, 1324, 1645
BstMAI GTCTC 1 cut(s) 1500
BstMBI GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
BstMCI CGRYCG 1 cut(s) 470
BstMWI GCNNNNNNNGC 3 cut(s) 209, 1339, 1611
BstNI CCWGG 1 cut(s) 1203
BstNSI RCATGY 2 cut(s) 1128, 1654
BstPAI GACNNNNGTC 1 cut(s) 1174
BstPI GGTNACC 2 cut(s) 522, 632
BstSCI CCNGG 1 cut(s) 1201
BstSNI TACGTA 1 cut(s) 736
BstV1I GCAGC 3 cut(s) 196, 412, 1623
BstV2I GAAGAC 1 cut(s) 283
BstX2I RGATCY 5 cut(s) 283, 360, 668, 1321, 1642
BstXI CCANNNNNNTGG 3 cut(s) 373, 1273, 1653
BstYI RGATCY 5 cut(s) 283, 360, 668, 1321, 1642
BstZ17I GTATAC 1 cut(s) 766
BsuRI GGCC 3 cut(s) 99, 1256, 1333
BtgI CCRYGG 2 cut(s) 769, 1251
BtsCI GGATG 5 cut(s) 150, 282, 352, 691, 874
BtsI GCAGTG 2 cut(s) 943, 1084
BtsIMutI CAGTG 5 cut(s) 20, 145, 680, 943, 1084
BveI ACCTGC 1 cut(s) 1438
Cac8I GCNNGC 2 cut(s) 423, 1442
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 5 cut(s) 97, 603, 1294, 1332, 1528
Csp6I GTAC 5 cut(s) 504, 695, 737, 802, 925
CspAI ACCGGT 1 cut(s) 500
CviQI GTAC 5 cut(s) 504, 695, 737, 802, 925
DdeI CTNAG 4 cut(s) 161, 861, 894, 957
DpnI GATC 7 cut(s) 285, 362, 670, 887, 1221, 1323, 1644
DpnII GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
DrdI GACNNNNNNGTC 1 cut(s) 977
DseDI GACNNNNNNGTC 1 cut(s) 977
Eam1104I CTCTTC 2 cut(s) 1481, 1498
EarI CTCTTC 2 cut(s) 1481, 1498
EciI GGCGGA 2 cut(s) 84, 666
Eco105I TACGTA 1 cut(s) 736
Eco130I CCWWGG 2 cut(s) 769, 1251
Eco47I GGWCC 3 cut(s) 603, 1294, 1528
Eco91I GGTNACC 2 cut(s) 522, 632
EcoO109I RGGNCCY 2 cut(s) 97, 1332
EcoO65I GGTNACC 2 cut(s) 522, 632
EcoRII CCWGG 1 cut(s) 1201
EcoT14I CCWWGG 2 cut(s) 769, 1251
ErhI CCWWGG 2 cut(s) 769, 1251
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FaqI GGGAC 1 cut(s) 1524
FauNDI CATATG 1 cut(s) 1600
FblI GTMKAC 3 cut(s) 388, 765, 1178
Fnu4HI GCNGC 4 cut(s) 210, 426, 429, 1612
FokI GGATG 5 cut(s) 157, 289, 359, 698, 881
Fsp4HI GCNGC 4 cut(s) 210, 426, 429, 1612
FspBI CTAG 6 cut(s) 191, 273, 320, 1434, 1548, 1608
GluI GCNGC 4 cut(s) 210, 426, 429, 1612
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 3 cut(s) 99, 1256, 1333
HapII CCGG 3 cut(s) 501, 512, 637
HincII GTYRAC 3 cut(s) 1179, 1317, 1659
HindII GTYRAC 3 cut(s) 1179, 1317, 1659
HindIII AAGCTT 1 cut(s) 1302
HinfI GANTC 5 cut(s) 622, 702, 971, 1003, 1019
HpaI GTTAAC 1 cut(s) 1317
HpaII CCGG 3 cut(s) 501, 512, 637
HphI GGTGA 8 cut(s) 94, 346, 368, 610, 631, 644, 889, 1060
Hpy188I TCNGA 7 cut(s) 396, 491, 591, 652, 895, 986, 1099
Hpy188III TCNNGA 2 cut(s) 1007, 1477
Hpy99I CGWCG 2 cut(s) 591, 722
HpyAV CCTTC 1 cut(s) 1457
HpyCH4III ACNGT 6 cut(s) 140, 435, 508, 554, 629, 817
HpyCH4IV ACGT 1 cut(s) 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 1339, 1611
HpyF3I CTNAG 4 cut(s) 161, 861, 894, 957
HpySE526I ACGT 1 cut(s) 735
KpnI GGTACC 2 cut(s) 507, 698
KspAI GTTAAC 1 cut(s) 1317
Kzo9I GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
Lsp1109I GCAGC 3 cut(s) 196, 412, 1623
LweI GCATC 1 cut(s) 937
MaeI CTAG 6 cut(s) 191, 273, 320, 1434, 1548, 1608
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 3 cut(s) 522, 623, 632
MalI GATC 7 cut(s) 285, 362, 670, 887, 1221, 1323, 1644
MboI GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
MboII GAAGA 8 cut(s) 283, 389, 469, 956, 1379, 1498, 1515, 1576
MfeI CAATTG 1 cut(s) 1233
MflI RGATCY 5 cut(s) 283, 360, 668, 1321, 1642
MluCI AATT 6 cut(s) 257, 492, 730, 1183, 1233, 1396
MlyI GAGTC 3 cut(s) 631, 965, 1012
MmeI TCCRAC 3 cut(s) 569, 630, 1064
MroXI GAANNNNTTC 2 cut(s) 476, 1211
MseI TTAA 5 cut(s) 260, 351, 1316, 1392, 1575
MslI CAYNNNNRTG 3 cut(s) 371, 630, 986
MspI CCGG 3 cut(s) 501, 512, 637
MspR9I CCNGG 1 cut(s) 1203
MunI CAATTG 1 cut(s) 1233
MvaI CCWGG 1 cut(s) 1203
MwoI GCNNNNNNNGC 3 cut(s) 209, 1339, 1611
NcoI CCATGG 2 cut(s) 769, 1251
NdeI CATATG 1 cut(s) 1600
NdeII GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
NlaIV GGNNCC 8 cut(s) 505, 696, 1140, 1296, 1334, 1366, 1530, 1644
NmuCI GTSAC 2 cut(s) 623, 632
NspI RCATGY 2 cut(s) 1128, 1654
OliI CACNNNNGTG 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 476, 1211
PfeI GAWTC 2 cut(s) 702, 1019
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 4 cut(s) 211, 427, 430, 1613
PleI GAGTC 3 cut(s) 630, 965, 1011
PpsI GAGTC 3 cut(s) 630, 965, 1011
Ppu21I YACGTR 1 cut(s) 736
PshAI GACNNNNGTC 1 cut(s) 1174
PshBI ATTAAT 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 1201
PspEI GGTNACC 2 cut(s) 522, 632
PspGI CCWGG 1 cut(s) 1201
PspN4I GGNNCC 8 cut(s) 505, 696, 1140, 1296, 1334, 1366, 1530, 1644
PspPI GGNCC 5 cut(s) 97, 603, 1294, 1332, 1528
PsuI RGATCY 5 cut(s) 283, 360, 668, 1321, 1642
RsaI GTAC 5 cut(s) 505, 696, 738, 803, 926
RsaNI GTAC 5 cut(s) 504, 695, 737, 802, 925
RseI CAYNNNNRTG 3 cut(s) 371, 630, 986
SalI GTCGAC 1 cut(s) 1177
SaqAI TTAA 5 cut(s) 260, 351, 1316, 1392, 1575
SatI GCNGC 4 cut(s) 210, 426, 429, 1612
Sau3AI GATC 7 cut(s) 283, 360, 668, 885, 1219, 1321, 1642
Sau96I GGNCC 5 cut(s) 97, 603, 1294, 1332, 1528
SchI GAGTC 3 cut(s) 631, 965, 1012
ScrFI CCNGG 1 cut(s) 1203
SfaNI GCATC 1 cut(s) 937
SinI GGWCC 3 cut(s) 603, 1294, 1528
SmiMI CAYNNNNRTG 3 cut(s) 371, 630, 986
SnaBI TACGTA 1 cut(s) 736
SpeI ACTAGT 1 cut(s) 319
Sse9I AATT 6 cut(s) 257, 492, 730, 1183, 1233, 1396
SsiI CCGC 3 cut(s) 69, 428, 677
SspMI CTAG 6 cut(s) 191, 273, 320, 1434, 1548, 1608
StyD4I CCNGG 1 cut(s) 1201
StyI CCWWGG 2 cut(s) 769, 1251
TaaI ACNGT 6 cut(s) 140, 435, 508, 554, 629, 817
TaiI ACGT 1 cut(s) 738
TaqI TCGA 4 cut(s) 700, 705, 974, 1178
TaqII GACCGA 1 cut(s) 484
TasI AATT 6 cut(s) 257, 492, 730, 1183, 1233, 1396
TatI WGTACW 1 cut(s) 801
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 2 cut(s) 702, 1019
Tru1I TTAA 5 cut(s) 260, 351, 1316, 1392, 1575
Tru9I TTAA 5 cut(s) 260, 351, 1316, 1392, 1575
TscAI CASTG 5 cut(s) 20, 145, 687, 943, 1084
TseFI GTSAC 2 cut(s) 623, 632
TseI GCWGC 3 cut(s) 209, 425, 1611
Tsp45I GTSAC 2 cut(s) 623, 632
TspDTI ATGAA 8 cut(s) 161, 236, 702, 1011, 1081, 1298, 1443, 1499
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 5 cut(s) 20, 145, 687, 943, 1084
VpaK11BI GGWCC 3 cut(s) 603, 1294, 1528
VspI ATTAAT 1 cut(s) 260
XceI RCATGY 2 cut(s) 1128, 1654
XcmI CCANNNNNNNNNTGG 1 cut(s) 687
XmiI GTMKAC 3 cut(s) 388, 765, 1178
XmnI GAANNNNTTC 2 cut(s) 476, 1211
XspI CTAG 6 cut(s) 191, 273, 320, 1434, 1548, 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.