MD15G1135500.v1.1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017232
Physical Location & Seq
Reverse (-)
9862666 .. 9864109
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1135500.v1.1.491

Sequence Viewer

Length: 912 bp
ATGATCAAGAGCGCATTCAGAGCTGATATGAAGTTTACTGTGTTTTTATGTTATAAAAACACAGTAGCTTTGTGTGCTCTACTTATAGCGGCACTCTACAGCAGACAGAGACCTCCTGTTCCCAAGGGTCGTCTCTTAGCTTGGATCATGTGCTTGGCCGTATTCGAGCCATTTTTGGGGCAACTTGCTGTGTTTGCTGGACTACGGTTCACGCCGGCCAGTTTCTCTAGTTGTATTATCGCAGTTGGCCCGGCCTTGACATTCATAGTCTCGTGGTATCACGGGTTGGAGACGGTCCACATCCATGAGAGACCTAGTCAAGCAAAATTGCTGGGGGTGTTGATGGTGATTGGTGGAGCCATAGTTGTGAGTCTGATTGATGGCCCCACCATTACCATTGCGCATGCCTCCACTAGCAGTGGCCACCCTTACAAGCTATCTAATAATTGGGTGAGAGGTCCACTGTTAGTCGGGATTTCAGTTATTCTTTCGGTTTGCTACAACTTGTTGATGCAAGAGACTACTACGAAGTACCCTGACTTCCCATTAGTTTGGCTTAATGCATACGTTGTCGCCATTGGAGCGCTGATGGACACTATAGTGGCCGTCGCGCTTACTGGGAGGACTACATATGCATGGATGCTGGGTTTGAACGTGAAGCTGGCATGCTACCTATACTCGGGTGTGGTGGTTTCTGCATTCACGGCATGGCTACAGAGCCGGGTGATCCGGATGAGAGACGCGGTCTTTTTGACCTCCTTTAGTCCCCTAGCCACCGTTGTTGTAATGGTAATAGGGTTTACAATACTAAAGGAAGAACTTGACAGTGGGAGCATTGCTGGAGGTGGTGTTATAGTGATAGGCCTTTTCGTTTTCCATTGGGGCAGGAGGCAGCCAGCACCCGACGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

304

Amino Acids

32.92

Weight (kDa)

9.39

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 7 - 124 5.3e-12 EamA-like transporter family
EamA PF00892 153 - 291 2e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24890 FvH4_1g24890
malus_domestica MD15G1135500.v1.1
prunus_persica Prupe.4G273500_v2.0.a1
pyrus_communis pycom15g12280
rosa_chinensis RchiOBHm_Chr2g0122551
rosa_laevigata RLG00000018686
rosa_multiflora Rmu_sc0003832.1_g000004
rosa_roxburghii Rroxscaffold_2G00121480
rosa_samantha Rh2AG298100 Rh2BG306900 Rh2CG285000 Rh2DG321700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 54
Acc16I TGCGCA 1 cut(s) 402
AccII CGCG 3 cut(s) 611, 743, 908
AccIII TCCGGA 1 cut(s) 729
AciI CCGC 2 cut(s) 89, 743
AclWI GGATC 2 cut(s) 152, 721
AcoI YGGCCR 4 cut(s) 156, 216, 421, 603
AfaI GTAC 1 cut(s) 533
AfeI AGCGCT 1 cut(s) 585
AfiI CCNNNNNNNGG 2 cut(s) 176, 679
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 1 cut(s) 652
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 5 cut(s) 23, 68, 140, 436, 661
AluI AGCT 5 cut(s) 23, 68, 140, 436, 661
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 7 cut(s) 103, 137, 274, 284, 304, 512, 732
AlwI GGATC 2 cut(s) 152, 721
Ama87I CYCGRG 1 cut(s) 679
Aor13HI TCCGGA 1 cut(s) 729
Aor51HI AGCGCT 1 cut(s) 585
AoxI GGCC 8 cut(s) 156, 216, 247, 252, 382, 421, 603, 862
ApeKI GCWGC 1 cut(s) 892
AspLEI GCGC 4 cut(s) 14, 403, 586, 613
AspS9I GGNCC 4 cut(s) 248, 295, 383, 458
AsuC2I CCSGG 2 cut(s) 251, 722
AsuHPI GGTGA 3 cut(s) 358, 463, 736
AvaI CYCGRG 1 cut(s) 679
AvaII GGWCC 2 cut(s) 295, 458
BalI TGGCCA 1 cut(s) 423
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
BauI CACGAG 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 1 cut(s) 904
BccI CCATC 3 cut(s) 337, 374, 583
BceAI ACGGC 3 cut(s) 143, 590, 720
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 251, 722
BcoDI GTCTC 7 cut(s) 103, 137, 274, 284, 304, 512, 732
BfaI CTAG 4 cut(s) 228, 315, 414, 770
BfmI CTRYAG 3 cut(s) 97, 597, 713
BfoI RGCGCY 1 cut(s) 587
BisI GCNGC 2 cut(s) 90, 893
BlsI GCNGC 2 cut(s) 91, 894
Bme1390I CCNGG 2 cut(s) 251, 722
Bme18I GGWCC 2 cut(s) 295, 458
BmeT110I CYCGRG 1 cut(s) 679
BmgT120I GGNCC 4 cut(s) 248, 295, 383, 458
BmiI GGNNCC 2 cut(s) 358, 385
BmrFI CCNGG 2 cut(s) 251, 722
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 2 cut(s) 501, 630
BmuI ACTGGG 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 861
BpuMI CCSGG 2 cut(s) 251, 722
BsaI GGTCTC 2 cut(s) 103, 304
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 1 cut(s) 729
BsaXI ACNNNNNCTCC 4 cut(s) 348, 378, 834, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 679
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 219, 622
Bse3DI GCAATG 2 cut(s) 396, 834
BseAI TCCGGA 1 cut(s) 729
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 3 cut(s) 300, 645, 738
BseLI CCNNNNNNNGG 2 cut(s) 176, 679
BseMI GCAATG 2 cut(s) 396, 834
BseNI ACTGG 2 cut(s) 219, 622
BseXI GCAGC 1 cut(s) 904
BseYI CCCAGC 2 cut(s) 331, 643
Bsh1236I CGCG 3 cut(s) 611, 743, 908
BshFI GGCC 8 cut(s) 158, 218, 249, 254, 384, 423, 605, 864
BsiHKAI GWGCWC 1 cut(s) 79
BsiHKCI CYCGRG 1 cut(s) 679
BsiSI CCGG 4 cut(s) 215, 251, 721, 730
BslFI GGGAC 1 cut(s) 750
BslI CCNNNNNNNGG 2 cut(s) 176, 679
BsmAI GTCTC 7 cut(s) 103, 137, 274, 284, 304, 512, 732
BsmBI CGTCTC 3 cut(s) 137, 284, 732
BsmFI GGGAC 1 cut(s) 750
BsmI GAATGC 2 cut(s) 14, 698
BsnI GGCC 8 cut(s) 158, 218, 249, 254, 384, 423, 605, 864
Bso31I GGTCTC 2 cut(s) 103, 304
BsoBI CYCGRG 1 cut(s) 679
Bsp1286I GDGCHC 1 cut(s) 79
Bsp13I TCCGGA 1 cut(s) 729
Bsp143I GATC 3 cut(s) 3, 144, 726
BspACI CCGC 2 cut(s) 89, 743
BspANI GGCC 8 cut(s) 158, 218, 249, 254, 384, 423, 605, 864
BspEI TCCGGA 1 cut(s) 729
BspFNI CGCG 3 cut(s) 611, 743, 908
BspLI GGNNCC 2 cut(s) 358, 385
BspPI GGATC 2 cut(s) 152, 721
BspTNI GGTCTC 2 cut(s) 103, 304
BsrDI GCAATG 2 cut(s) 396, 834
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 2 cut(s) 219, 622
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 3 cut(s) 3, 144, 726
BssSI CACGAG 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 271
Bst4CI ACNGT 7 cut(s) 40, 64, 207, 295, 465, 778, 827
BstC8I GCNNGC 5 cut(s) 216, 405, 663, 667, 897
BstDEI CTNAG 1 cut(s) 136
BstF5I GGATG 3 cut(s) 300, 645, 738
BstFNI CGCG 3 cut(s) 611, 743, 908
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 4 cut(s) 14, 403, 586, 613
BstKTI GATC 3 cut(s) 6, 147, 729
BstMAI GTCTC 7 cut(s) 103, 137, 274, 284, 304, 512, 732
BstMBI GATC 3 cut(s) 3, 144, 726
BstMWI GCNNNNNNNGC 6 cut(s) 20, 74, 194, 581, 704, 905
BstNSI RCATGY 2 cut(s) 407, 669
BstSCI CCNGG 2 cut(s) 249, 720
BstSFI CTRYAG 3 cut(s) 97, 597, 713
BstUI CGCG 3 cut(s) 611, 743, 908
BstV1I GCAGC 1 cut(s) 904
BstXI CCANNNNNNTGG 1 cut(s) 552
BsuRI GGCC 8 cut(s) 158, 218, 249, 254, 384, 423, 605, 864
BtsCI GGATG 3 cut(s) 300, 645, 738
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 3 cut(s) 424, 461, 832
Cac8I GCNNGC 5 cut(s) 216, 405, 663, 667, 897
CfoI GCGC 4 cut(s) 14, 403, 586, 613
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 4 cut(s) 248, 295, 383, 458
CseI GACGC 1 cut(s) 749
Csp6I GTAC 1 cut(s) 532
CspCI CAANNNNNGTGG 2 cut(s) 763, 798
CviAII CATG 6 cut(s) 148, 305, 404, 636, 666, 708
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 1 cut(s) 136
DpnI GATC 3 cut(s) 5, 146, 728
DpnII GATC 3 cut(s) 3, 144, 726
EaeI YGGCCR 4 cut(s) 156, 216, 421, 603
Eco130I CCWWGG 1 cut(s) 123
Eco147I AGGCCT 1 cut(s) 864
Eco31I GGTCTC 2 cut(s) 103, 304
Eco47I GGWCC 2 cut(s) 295, 458
Eco47III AGCGCT 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 2 cut(s) 565, 637
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 3 cut(s) 137, 284, 732
FaeI CATG 6 cut(s) 151, 308, 407, 639, 669, 711
FaqI GGGAC 1 cut(s) 750
FatI CATG 6 cut(s) 147, 304, 403, 635, 665, 707
FauNDI CATATG 1 cut(s) 631
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 90, 893
FokI GGATG 3 cut(s) 287, 652, 745
Fsp4HI GCNGC 2 cut(s) 90, 893
FspBI CTAG 4 cut(s) 228, 315, 414, 770
FspI TGCGCA 1 cut(s) 402
GlaI GCGC 4 cut(s) 13, 402, 585, 612
GluI GCNGC 2 cut(s) 90, 893
GsaI CCCAGC 2 cut(s) 335, 647
GsuI CTGGAG 1 cut(s) 861
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 8 cut(s) 158, 218, 249, 254, 384, 423, 605, 864
HapII CCGG 4 cut(s) 215, 251, 721, 730
HgaI GACGC 1 cut(s) 749
HhaI GCGC 4 cut(s) 14, 403, 586, 613
Hin1II CATG 6 cut(s) 151, 308, 407, 639, 669, 711
Hin6I GCGC 4 cut(s) 12, 401, 584, 611
HinP1I GCGC 4 cut(s) 12, 401, 584, 611
HinfI GANTC 1 cut(s) 370
HpaII CCGG 4 cut(s) 215, 251, 721, 730
HphI GGTGA 3 cut(s) 358, 463, 736
Hpy166II GTNNAC 5 cut(s) 36, 210, 298, 461, 801
Hpy188I TCNGA 2 cut(s) 20, 375
Hpy188III TCNNGA 3 cut(s) 7, 472, 730
Hpy8I GTNNAC 5 cut(s) 36, 210, 298, 461, 801
Hpy99I CGWCG 2 cut(s) 611, 908
HpyCH4III ACNGT 7 cut(s) 40, 64, 207, 295, 465, 778, 827
HpyCH4IV ACGT 2 cut(s) 567, 654
HpyCH4V TGCA 4 cut(s) 514, 563, 635, 698
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 74, 194, 581, 704, 905
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 2 cut(s) 567, 654
Hsp92II CATG 6 cut(s) 151, 308, 407, 639, 669, 711
HspAI GCGC 4 cut(s) 12, 401, 584, 611
Kpn2I TCCGGA 1 cut(s) 729
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 144, 726
LmnI GCTCC 3 cut(s) 356, 581, 831
Lsp1109I GCAGC 1 cut(s) 904
LweI GCATC 2 cut(s) 501, 630
MaeI CTAG 4 cut(s) 228, 315, 414, 770
MaeII ACGT 2 cut(s) 567, 654
MalI GATC 3 cut(s) 5, 146, 728
MboI GATC 3 cut(s) 3, 144, 726
MboII GAAGA 1 cut(s) 827
MhlI GDGCHC 1 cut(s) 79
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 2 cut(s) 326, 445
MluI ACGCGT 1 cut(s) 906
MluNI TGGCCA 1 cut(s) 423
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 7 cut(s) 123, 418, 449, 615, 766, 836, 882
Mox20I TGGCCA 1 cut(s) 423
Mph1103I ATGCAT 2 cut(s) 565, 637
MroI TCCGGA 1 cut(s) 729
MroNI GCCGGC 1 cut(s) 214
MscI TGGCCA 1 cut(s) 423
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 4 cut(s) 303, 365, 599, 634
Msp20I TGGCCA 1 cut(s) 423
MspI CCGG 4 cut(s) 215, 251, 721, 730
MspR9I CCNGG 2 cut(s) 251, 722
Mva1269I GAATGC 2 cut(s) 14, 698
MvnI CGCG 3 cut(s) 611, 743, 908
MwoI GCNNNNNNNGC 6 cut(s) 20, 74, 194, 581, 704, 905
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 2 cut(s) 251, 722
NdeI CATATG 1 cut(s) 631
NdeII GATC 3 cut(s) 3, 144, 726
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 6 cut(s) 151, 308, 407, 639, 669, 711
NlaIV GGNNCC 2 cut(s) 358, 385
NsbI TGCGCA 1 cut(s) 402
NsiI ATGCAT 2 cut(s) 565, 637
NspI RCATGY 2 cut(s) 407, 669
OliI CACNNNNGTG 1 cut(s) 599
PaeI GCATGC 2 cut(s) 407, 669
PceI AGGCCT 1 cut(s) 864
PctI GAATGC 2 cut(s) 14, 698
PdiI GCCGGC 1 cut(s) 216
PflFI GACNNNGTC 2 cut(s) 315, 743
PkrI GCNGC 2 cut(s) 91, 894
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PsiI TTATAA 1 cut(s) 54
PspFI CCCAGC 2 cut(s) 331, 643
PspN4I GGNNCC 2 cut(s) 358, 385
PspPI GGNCC 4 cut(s) 248, 295, 383, 458
PsyI GACNNNGTC 2 cut(s) 315, 743
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
RseI CAYNNNNRTG 4 cut(s) 303, 365, 599, 634
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 2 cut(s) 90, 893
Sau3AI GATC 3 cut(s) 3, 144, 726
Sau96I GGNCC 4 cut(s) 248, 295, 383, 458
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 251, 722
SduI GDGCHC 1 cut(s) 79
SfaNI GCATC 2 cut(s) 501, 630
SfcI CTRYAG 3 cut(s) 97, 597, 713
SinI GGWCC 2 cut(s) 295, 458
SmiMI CAYNNNNRTG 4 cut(s) 303, 365, 599, 634
SphI GCATGC 2 cut(s) 407, 669
Sse9I AATT 2 cut(s) 326, 445
SseBI AGGCCT 1 cut(s) 864
SsiI CCGC 2 cut(s) 89, 743
SspMI CTAG 4 cut(s) 228, 315, 414, 770
StuI AGGCCT 1 cut(s) 864
StyD4I CCNGG 2 cut(s) 249, 720
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 7 cut(s) 40, 64, 207, 295, 465, 778, 827
TaiI ACGT 2 cut(s) 570, 657
TaqI TCGA 1 cut(s) 165
TasI AATT 2 cut(s) 326, 445
TauI GCSGC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 3 cut(s) 424, 468, 832
TseI GCWGC 1 cut(s) 892
TspDTI ATGAA 2 cut(s) 44, 253
TspRI CASTG 3 cut(s) 424, 468, 832
Tth111I GACNNNGTC 2 cut(s) 315, 743
VpaK11BI GGWCC 2 cut(s) 295, 458
XceI RCATGY 2 cut(s) 407, 669
XspI CTAG 4 cut(s) 228, 315, 414, 770
Zsp2I ATGCAT 2 cut(s) 565, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.