pycom15g12280

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017232
Physical Location & Seq
Forward (+)
8388063 .. 8389853
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12280.4

Sequence Viewer

Length: 1188 bp
ATGCTGCATCTAGGATTGTTGTGTAAAAATCGTATTAATTCTTGGACTTTAAGATCTCTGGTTATCAAGTATGAACTGAACTTTTATTTGTTAATTCTGCAGAGAAACAAGCAGCTCAAGATGCTAACTCTACAAGATTTGCCACTTTGGATGTTCCCGCATCTTACTCCGTTGTGCGGAAGAATGAAAGGAGCTGTCCCGTTTATTCTGGGCTTTTTGATCCAACTACTCATCGGGGGCAACTCCATTGTCATCAAGATGGCATTCAGAGCTGACATGAAGTTCACTGTGTTTTTATGCTATAAAAACACAGTGGCTTTCTTCGCTTTGCTTGTAGCCGCACTCTACAGCAAACGAAGACCTCCCGTTCCAAAGGGTTGGCTTTTAGCCTACATCATCTGTTTGGGCATGCTTGAGCCATTTTTGGGACAGCTTGCTGTTTTTGCGGGATTGCGGTTCACGCCGGCCAGTTTCTCTAGTTGCATCATCGCAGCTGGTCCGGCCTTGACCTTCTTTCTCTCGTGGTGGTTCAAGTCAGAAAAAGTCAATATTCGTGAGAGGCCTAGCCAAGCAAAACTGCTTGGTGTGTTGATGGTGATCACTGGTGGCATAGTTGTCAGCCAGATTGACGGCCCCACTATCACCATTGCGCACTCCTCTACTAGTCATGGTGGCTTGTTGAAGTACACCAAAAATTGGGTGAGAGGTCCAGTGCTAGTCGGAACTGCAGTGGTCCTATCGGTGTGCTACAACTTACTAATGGTAAGCCAACGGACCACCAATGAGTACCCTGACTTCCCGTTGATTTGGCTGAATGCGTATGCTGTCTCCATTGGAGCGTTGATGGACACCATAGTGGCCGTTGCGCTTACCGGGAGTACAACATATGCATGGATGCTAGGGTTGAACGTGAAGTTGGCATGCTACCTGTATTCGGTGAGTTTTGGGGGCCTGGTTGTTTCTGCATTTACGGCATGGCTACAGAGCCTAGTGATCAAGATGAGAGGTGCCGTCTTTCTGTCATTTTTTAGCCCCCTAGCCACGGTTATTGTCATGGTACTAGGTGTTACAGTACTGAAGGAGGATGTTGACACTGGGAGCATTGTGGGAGGCTGCGTTATTGTGACTGGCCTGTTTGTCTTTCAATGGGCCAAGTGGCGGCAAACGCAAAGCGAGTTGCCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

396

Amino Acids

43.7

Weight (kDa)

9.75

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24890 FvH4_1g24890
malus_domestica MD15G1135500.v1.1
prunus_persica Prupe.4G273500_v2.0.a1
pyrus_communis pycom15g12280
rosa_chinensis RchiOBHm_Chr2g0122551
rosa_laevigata RLG00000018686
rosa_multiflora Rmu_sc0003832.1_g000004
rosa_roxburghii Rroxscaffold_2G00121480
rosa_samantha Rh2AG298100 Rh2BG306900 Rh2CG285000 Rh2DG321700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 651
AccB1I GGYRCC 1 cut(s) 1007
AccB7I CCANNNNNTGG 1 cut(s) 696
AciI CCGC 6 cut(s) 158, 177, 339, 446, 454, 1159
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 2 cut(s) 465, 858
AcuI CTGAAG 1 cut(s) 1097
AfaI GTAC 5 cut(s) 686, 788, 880, 1059, 1074
AfiI CCNNNNNNNGG 6 cut(s) 176, 425, 696, 934, 1042, 1158
AgsI TTSAA 4 cut(s) 532, 682, 907, 1145
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 1 cut(s) 951
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AleI CACNNNNGTG 1 cut(s) 854
AluBI AGCT 5 cut(s) 115, 194, 272, 433, 494
AluI AGCT 5 cut(s) 115, 194, 272, 433, 494
Alw26I GTCTC 1 cut(s) 832
AlwI GGATC 1 cut(s) 214
AoxI GGCC 8 cut(s) 465, 501, 560, 631, 858, 949, 1129, 1149
ApeKI GCWGC 4 cut(s) 4, 112, 491, 1113
AseI ATTAAT 1 cut(s) 36
AspLEI GCGC 2 cut(s) 652, 868
AspS9I GGNCC 7 cut(s) 497, 632, 707, 733, 774, 949, 1149
AsuC2I CCSGG 1 cut(s) 874
AsuHPI GGTGA 4 cut(s) 607, 634, 712, 949
AvaII GGWCC 4 cut(s) 497, 707, 733, 774
BanI GGYRCC 1 cut(s) 1007
BarI GAAGNNNNNNTAC 2 cut(s) 779, 811
BauI CACGAG 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 124, 503, 1100
BccI CCATC 3 cut(s) 253, 586, 838
BceAI ACGGC 4 cut(s) 646, 845, 987, 995
BciT130I CCWGG 1 cut(s) 953
BclI TGATCA 2 cut(s) 597, 993
BcnI CCSGG 1 cut(s) 874
BcoDI GTCTC 1 cut(s) 832
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 9 cut(s) 11, 477, 564, 663, 716, 899, 989, 1037, 1061
BfmI CTRYAG 4 cut(s) 98, 346, 726, 980
BglII AGATCT 1 cut(s) 53
BisI GCNGC 6 cut(s) 5, 113, 339, 492, 1114, 1160
BlsI GCNGC 6 cut(s) 6, 114, 340, 493, 1115, 1161
BmcAI AGTACT 1 cut(s) 1074
Bme1390I CCNGG 2 cut(s) 874, 953
Bme18I GGWCC 4 cut(s) 497, 707, 733, 774
BmgT120I GGNCC 7 cut(s) 497, 632, 707, 733, 774, 949, 1149
BmiI GGNNCC 3 cut(s) 634, 950, 1009
BmrFI CCNGG 2 cut(s) 874, 953
BmrI ACTGGG 1 cut(s) 1104
BmsI GCATC 5 cut(s) 16, 111, 169, 492, 885
BmuI ACTGGG 1 cut(s) 1104
BpiI GAAGAC 1 cut(s) 364
BpuEI CTTGAG 2 cut(s) 101, 434
BpuMI CCSGG 1 cut(s) 874
BsaBI GATNNNNATC 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 1041
BsaXI ACNNNNNCTCC 2 cut(s) 1101, 1131
Bsc4I CCNNNNNNNGG 6 cut(s) 176, 425, 696, 934, 1042, 1158
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 5 cut(s) 468, 607, 710, 1099, 1132
Bse3DI GCAATG 1 cut(s) 645
Bse8I GATNNNNATC 1 cut(s) 596
BseBI CCWGG 1 cut(s) 953
BseDI CCNNGG 1 cut(s) 1041
BseGI GGATG 3 cut(s) 156, 900, 1090
BseJI GATNNNNATC 1 cut(s) 596
BseLI CCNNNNNNNGG 6 cut(s) 176, 425, 696, 934, 1042, 1158
BseMI GCAATG 1 cut(s) 645
BseNI ACTGG 5 cut(s) 468, 607, 710, 1099, 1132
BseRI GAGGAG 1 cut(s) 646
BseXI GCAGC 3 cut(s) 124, 503, 1100
BshFI GGCC 8 cut(s) 467, 503, 562, 633, 860, 951, 1131, 1151
BshNI GGYRCC 1 cut(s) 1007
BsiSI CCGG 3 cut(s) 464, 500, 873
BslFI GGGAC 2 cut(s) 182, 441
BslI CCNNNNNNNGG 6 cut(s) 176, 425, 696, 934, 1042, 1158
BsmAI GTCTC 1 cut(s) 832
BsmFI GGGAC 2 cut(s) 182, 441
BsmI GAATGC 2 cut(s) 263, 820
BsnI GGCC 8 cut(s) 467, 503, 562, 633, 860, 951, 1131, 1151
Bsp143I GATC 4 cut(s) 53, 219, 597, 993
BspACI CCGC 6 cut(s) 158, 177, 339, 446, 454, 1159
BspANI GGCC 8 cut(s) 467, 503, 562, 633, 860, 951, 1131, 1151
BspLI GGNNCC 3 cut(s) 634, 950, 1009
BspMAI CTGCAG 2 cut(s) 102, 730
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 1007
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 5 cut(s) 468, 607, 710, 1099, 1132
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 1 cut(s) 1041
BssMI GATC 4 cut(s) 53, 219, 597, 993
BssSI CACGAG 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 520
Bst2UI CCWGG 1 cut(s) 953
Bst4CI ACNGT 4 cut(s) 289, 313, 1045, 1072
BstC8I GCNNGC 4 cut(s) 410, 435, 465, 922
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 3 cut(s) 156, 900, 1090
BstHHI GCGC 2 cut(s) 652, 868
BstKTI GATC 4 cut(s) 56, 222, 600, 996
BstMAI GTCTC 1 cut(s) 832
BstMBI GATC 4 cut(s) 53, 219, 597, 993
BstMWI GCNNNNNNNGC 8 cut(s) 121, 269, 323, 443, 460, 500, 971, 1165
BstNI CCWGG 1 cut(s) 953
BstNSI RCATGY 2 cut(s) 412, 924
BstSCI CCNGG 2 cut(s) 872, 951
BstSFI CTRYAG 4 cut(s) 98, 346, 726, 980
BstV1I GCAGC 3 cut(s) 124, 503, 1100
BstV2I GAAGAC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 53
BstXI CCANNNNNNTGG 1 cut(s) 378
BstYI RGATCY 1 cut(s) 53
BsuRI GGCC 8 cut(s) 467, 503, 562, 633, 860, 951, 1131, 1151
BtgI CCRYGG 1 cut(s) 1041
BtgZI GCGATG 1 cut(s) 472
BtsCI GGATG 3 cut(s) 156, 900, 1090
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 6 cut(s) 285, 318, 600, 717, 735, 1092
Cac8I GCNNGC 4 cut(s) 410, 435, 465, 922
CfoI GCGC 2 cut(s) 652, 868
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 7 cut(s) 497, 632, 707, 733, 774, 949, 1149
Csp6I GTAC 5 cut(s) 685, 787, 879, 1058, 1073
CspCI CAANNNNNGTGG 2 cut(s) 1030, 1065
CviAII CATG 7 cut(s) 277, 409, 668, 891, 921, 975, 1054
CviQI GTAC 5 cut(s) 685, 787, 879, 1058, 1073
DpnI GATC 4 cut(s) 55, 221, 599, 995
DpnII GATC 4 cut(s) 53, 219, 597, 993
EaeI YGGCCR 2 cut(s) 465, 858
Eco147I AGGCCT 1 cut(s) 562
Eco47I GGWCC 4 cut(s) 497, 707, 733, 774
Eco57I CTGAAG 1 cut(s) 1097
EcoO109I RGGNCCY 1 cut(s) 949
EcoRII CCWGG 1 cut(s) 951
EcoT22I ATGCAT 1 cut(s) 892
FaeI CATG 7 cut(s) 280, 412, 671, 894, 924, 978, 1057
FaqI GGGAC 2 cut(s) 182, 441
FatI CATG 7 cut(s) 276, 408, 667, 890, 920, 974, 1053
FauI CCCGC 2 cut(s) 165, 439
FauNDI CATATG 1 cut(s) 886
FbaI TGATCA 2 cut(s) 597, 993
Fnu4HI GCNGC 6 cut(s) 5, 113, 339, 492, 1114, 1160
FokI GGATG 3 cut(s) 163, 907, 1097
Fsp4HI GCNGC 6 cut(s) 5, 113, 339, 492, 1114, 1160
FspBI CTAG 9 cut(s) 11, 477, 564, 663, 716, 899, 989, 1037, 1061
FspI TGCGCA 1 cut(s) 651
GlaI GCGC 2 cut(s) 651, 867
GluI GCNGC 6 cut(s) 5, 113, 339, 492, 1114, 1160
HaeIII GGCC 8 cut(s) 467, 503, 562, 633, 860, 951, 1131, 1151
HapII CCGG 3 cut(s) 464, 500, 873
HhaI GCGC 2 cut(s) 652, 868
Hin1II CATG 7 cut(s) 280, 412, 671, 894, 924, 978, 1057
Hin6I GCGC 2 cut(s) 650, 866
HinP1I GCGC 2 cut(s) 650, 866
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HpaII CCGG 3 cut(s) 464, 500, 873
HphI GGTGA 4 cut(s) 607, 634, 712, 949
Hpy166II GTNNAC 4 cut(s) 285, 459, 687, 1090
Hpy188I TCNGA 3 cut(s) 269, 538, 722
Hpy188III TCNNGA 4 cut(s) 118, 256, 554, 997
Hpy8I GTNNAC 4 cut(s) 285, 459, 687, 1090
HpyAV CCTTC 2 cut(s) 520, 1072
HpyCH4III ACNGT 4 cut(s) 289, 313, 1045, 1072
HpyCH4IV ACGT 1 cut(s) 909
HpyCH4V TGCA 6 cut(s) 7, 100, 483, 728, 890, 965
HpyF10VI GCNNNNNNNGC 8 cut(s) 121, 269, 323, 443, 460, 500, 971, 1165
HpySE526I ACGT 1 cut(s) 909
Hsp92II CATG 7 cut(s) 280, 412, 671, 894, 924, 978, 1057
HspAI GCGC 2 cut(s) 650, 866
KroI GCCGGC 1 cut(s) 463
KroNI GCCGGC 1 cut(s) 465
Ksp22I TGATCA 2 cut(s) 597, 993
Kzo9I GATC 4 cut(s) 53, 219, 597, 993
LmnI GCTCC 3 cut(s) 191, 836, 1098
Lsp1109I GCAGC 3 cut(s) 124, 503, 1100
LweI GCATC 5 cut(s) 16, 111, 169, 492, 885
MaeI CTAG 9 cut(s) 11, 477, 564, 663, 716, 899, 989, 1037, 1061
MaeII ACGT 1 cut(s) 909
MaeIII GTNAC 2 cut(s) 1066, 1123
MalI GATC 4 cut(s) 55, 221, 599, 995
MboI GATC 4 cut(s) 53, 219, 597, 993
MboII GAAGA 3 cut(s) 192, 313, 369
MflI RGATCY 1 cut(s) 53
MluCI AATT 3 cut(s) 37, 93, 694
MmeI TCCRAC 2 cut(s) 247, 700
MnlI CCTC 7 cut(s) 372, 552, 667, 698, 998, 1075, 1103
Mph1103I ATGCAT 1 cut(s) 892
MroNI GCCGGC 1 cut(s) 463
MseI TTAA 3 cut(s) 36, 50, 92
MslI CAYNNNNRTG 3 cut(s) 257, 854, 889
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 3 cut(s) 464, 500, 873
MspR9I CCNGG 2 cut(s) 874, 953
Mva1269I GAATGC 2 cut(s) 263, 820
MvaI CCWGG 1 cut(s) 953
MwoI GCNNNNNNNGC 8 cut(s) 121, 269, 323, 443, 460, 500, 971, 1165
NaeI GCCGGC 1 cut(s) 465
NciI CCSGG 1 cut(s) 874
NdeI CATATG 1 cut(s) 886
NdeII GATC 4 cut(s) 53, 219, 597, 993
NgoMIV GCCGGC 1 cut(s) 463
NlaIII CATG 7 cut(s) 280, 412, 671, 894, 924, 978, 1057
NlaIV GGNNCC 3 cut(s) 634, 950, 1009
NmuCI GTSAC 1 cut(s) 1123
NsbI TGCGCA 1 cut(s) 651
NsiI ATGCAT 1 cut(s) 892
NspI RCATGY 2 cut(s) 412, 924
OliI CACNNNNGTG 1 cut(s) 854
PaeI GCATGC 2 cut(s) 412, 924
PceI AGGCCT 1 cut(s) 562
PctI GAATGC 2 cut(s) 263, 820
PdiI GCCGGC 1 cut(s) 465
PflMI CCANNNNNTGG 1 cut(s) 696
PkrI GCNGC 6 cut(s) 6, 114, 340, 493, 1115, 1161
PshBI ATTAAT 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 951
PspGI CCWGG 1 cut(s) 951
PspN4I GGNNCC 3 cut(s) 634, 950, 1009
PspPI GGNCC 7 cut(s) 497, 632, 707, 733, 774, 949, 1149
PstI CTGCAG 2 cut(s) 102, 730
PsuI RGATCY 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 5 cut(s) 686, 788, 880, 1059, 1074
RsaNI GTAC 5 cut(s) 685, 787, 879, 1058, 1073
RseI CAYNNNNRTG 3 cut(s) 257, 854, 889
SaqAI TTAA 3 cut(s) 36, 50, 92
SatI GCNGC 6 cut(s) 5, 113, 339, 492, 1114, 1160
Sau3AI GATC 4 cut(s) 53, 219, 597, 993
Sau96I GGNCC 7 cut(s) 497, 632, 707, 733, 774, 949, 1149
ScaI AGTACT 1 cut(s) 1074
ScrFI CCNGG 2 cut(s) 874, 953
SfaNI GCATC 5 cut(s) 16, 111, 169, 492, 885
SfcI CTRYAG 4 cut(s) 98, 346, 726, 980
SinI GGWCC 4 cut(s) 497, 707, 733, 774
SmiMI CAYNNNNRTG 3 cut(s) 257, 854, 889
SmlI CTYRAG 2 cut(s) 116, 413
SmoI CTYRAG 2 cut(s) 116, 413
SpeI ACTAGT 1 cut(s) 662
SphI GCATGC 2 cut(s) 412, 924
Sse9I AATT 3 cut(s) 37, 93, 694
SseBI AGGCCT 1 cut(s) 562
SsiI CCGC 6 cut(s) 158, 177, 339, 446, 454, 1159
SspI AATATT 1 cut(s) 550
SspMI CTAG 9 cut(s) 11, 477, 564, 663, 716, 899, 989, 1037, 1061
StuI AGGCCT 1 cut(s) 562
StyD4I CCNGG 2 cut(s) 872, 951
TaaI ACNGT 4 cut(s) 289, 313, 1045, 1072
TaiI ACGT 1 cut(s) 912
TasI AATT 3 cut(s) 37, 93, 694
TatI WGTACW 3 cut(s) 684, 878, 1072
TauI GCSGC 2 cut(s) 341, 1162
Tru1I TTAA 3 cut(s) 36, 50, 92
Tru9I TTAA 3 cut(s) 36, 50, 92
TscAI CASTG 6 cut(s) 292, 318, 607, 717, 735, 1099
TseFI GTSAC 1 cut(s) 1123
TseI GCWGC 4 cut(s) 4, 112, 491, 1113
Tsp45I GTSAC 1 cut(s) 1123
TspDTI ATGAA 3 cut(s) 87, 200, 293
TspGWI ACGGA 2 cut(s) 159, 787
TspRI CASTG 6 cut(s) 292, 318, 607, 717, 735, 1099
Van91I CCANNNNNTGG 1 cut(s) 696
VpaK11BI GGWCC 4 cut(s) 497, 707, 733, 774
VspI ATTAAT 1 cut(s) 36
XceI RCATGY 2 cut(s) 412, 924
XspI CTAG 9 cut(s) 11, 477, 564, 663, 716, 899, 989, 1037, 1061
ZrmI AGTACT 1 cut(s) 1074
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.