Rh2BG306900

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017232
Physical Location & Seq
Reverse (-)
35703558 .. 35705171
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG306900.1

Sequence Viewer

Length: 1347 bp
ATGTGGCGGCGCAGTATGACTTTGGCAGCTGCTCTGGGGTTAGAGGTGAGATCTCCATTTGCATGGAAGATTATAGCAGACATCAGGCTGGTCAGCTACGTATATTTGGGTGTCGTTGTTTCGGGTCTTGCTTTATTCCTCCAGATCACACTCACTAGGATGAGAGATCCAGTCTTCGCGGCTGCATTTAGCCCGATATCACTAGTCTTTGTTATGATGATGAGTTTGGTAGCATTGAGGGATGTTATACATCTTGGAAGGAGTCGGCATCCCTACCTGCACCACACTCATCACCTGCATCAGCCGAAGCAAAGGATAGTAGGAGAGGCAGATGAGTTATTTATACTAGAGCGGGGAGAGCAGGAGCCACCACAGCGTCCAGAGGCATGTCAGTCACAGGAGAGGTCAGAGACACAGCAAGCAAAGCTAGAGGCAGAGCTGCCAGAGCGATCGAGAGAGGCAGAGGTGAGAAAGCAGCTAGGACTAGAGCCAGTTGAGTCTAGACAAGAGAGGTCGGAGACACAGTCAGAGCGGCTGAGAGAGGCAGCACTGAGAAAGATAGAGATGCATGCACTAGAGCAGCCAGCGGTAGACACTTCTACACAGGAAAAGTTGGAGGTAGAGGAATCAGAGCAGCCAAGGCTGCCCGCACTAGAGCAGCCAGAGGCTGACAGGTCCTCGGAGACACAGTTAGTGCGGCCGAGAGAGGCAGAGCTTAGAGAGAGAGAGCAGCCAGCGATAGACACTTCTACACAGGAGAGGATAGAGGTAGAGGAGTCAGAGCAGCCAGTGCTACCAGCACTAGAGCAGCTAGAGGCTGACGAGTCTTCGGAGACACAGTTAGAGCGCTCGAGAGAGGCAGAGCAGCCAGCGATAGACACTTCTACACAGGAAAGGATAGATGTAGAGGAGTCAGAGCAGCCAGGGCTACCCGCACTAGAGCAGCTAGAGGCTGACGAGTCTTCGGAGACACAGTTAGAGTGCCTGAGAGAGGCAGAGCAGCCAGCGATAGATACTTCTACACAAGAGAAGATAGATGTAGAGGAGTCAGAGCAGCCAAGGCTACCTGCACTAGAGCAGCTAGAGGCTGACGAGTCTTCAGAGACACAGTTAGAGCGGCTGAGAGAGGCAGAGCTTAGAGAGCCAGAGCAGCTAGCGGTAGACACTTCTACACAGGATAGGATGGAGGTAGAGGAGTCAGAGCAGCCAGAGGCTGACGAGTCCTCGGAGACACAGTTAGAGCAGCTTAGAGAGGTAGAGCTTAGAGAGCCAGAGATGCCTGCACATGATTCCACACAAGATAGGCTTGAGGTAGAGCAGCTAGGACAGAGAGACCCAGGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

448

Amino Acids

51.28

Weight (kDa)

4.46

Isoelectric Point (pI)

75.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017232)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24890 FvH4_1g24890
malus_domestica MD15G1135500.v1.1
prunus_persica Prupe.4G273500_v2.0.a1
pyrus_communis pycom15g12280
rosa_chinensis RchiOBHm_Chr2g0122551
rosa_laevigata RLG00000018686
rosa_multiflora Rmu_sc0003832.1_g000004
rosa_roxburghii Rroxscaffold_2G00121480
rosa_samantha Rh2AG298100 Rh2BG306900 Rh2CG285000 Rh2DG321700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 303
Acc36I ACCTGC 3 cut(s) 285, 303, 1075
AccBSI CCGCTC 3 cut(s) 352, 532, 1117
AccI GTMKAC 2 cut(s) 591, 1161
AccII CGCG 1 cut(s) 179
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 1 cut(s) 698
AcuI CTGAAG 1 cut(s) 1083
AfeI AGCGCT 1 cut(s) 848
AfiI CCNNNNNNNGG 1 cut(s) 991
AhlI ACTAGT 1 cut(s) 202
AjnI CCWGG 2 cut(s) 922, 1336
Alw26I GTCTC 8 cut(s) 404, 512, 677, 827, 962, 1097, 1223, 1326
AlwI GGATC 1 cut(s) 161
AlwNI CAGNNNCTG 2 cut(s) 668, 1214
Ama87I CYCGRG 1 cut(s) 850
Aor51HI AGCGCT 1 cut(s) 848
AoxI GGCC 1 cut(s) 698
AspLEI GCGC 2 cut(s) 12, 849
AspS9I GGNCC 1 cut(s) 675
AsuHPI GGTGA 3 cut(s) 58, 284, 478
AsuNHI GCTAGC 1 cut(s) 1153
AvaI CYCGRG 1 cut(s) 850
AvaII GGWCC 1 cut(s) 675
BbsI GAAGAC 4 cut(s) 166, 819, 954, 1089
BccI CCATC 1 cut(s) 1177
BciT130I CCWGG 2 cut(s) 924, 1338
BcoDI GTCTC 8 cut(s) 404, 512, 677, 827, 962, 1097, 1223, 1326
BcuI ACTAGT 1 cut(s) 202
BfoI RGCGCY 1 cut(s) 850
BfuAI ACCTGC 3 cut(s) 285, 303, 1075
BglII AGATCT 1 cut(s) 50
Bme1390I CCNGG 2 cut(s) 924, 1338
Bme18I GGWCC 1 cut(s) 675
BmeT110I CYCGRG 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 675
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 2 cut(s) 924, 1338
BmsI GCATC 4 cut(s) 277, 307, 555, 1266
BmtI GCTAGC 1 cut(s) 1157
BpiI GAAGAC 4 cut(s) 166, 819, 954, 1089
BpmI CTGGAG 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 1328
BsaAI YACGTR 1 cut(s) 100
BsaI GGTCTC 1 cut(s) 1326
BsaJI CCNNGG 7 cut(s) 638, 678, 923, 1058, 1224, 1336, 1337
Bsc4I CCNNNNNNNGG 1 cut(s) 991
Bse1I ACTGG 3 cut(s) 170, 491, 788
BseBI CCWGG 2 cut(s) 924, 1338
BseDI CCNNGG 7 cut(s) 638, 678, 923, 1058, 1224, 1336, 1337
BseGI GGATG 4 cut(s) 165, 247, 268, 1188
BseLI CCNNNNNNNGG 1 cut(s) 991
BseMII CTCAG 4 cut(s) 527, 542, 977, 1112
BseNI ACTGG 3 cut(s) 170, 491, 788
BseRI GAGGAG 4 cut(s) 788, 923, 1058, 1208
BseX3I CGGCCG 1 cut(s) 698
BsgI GTGCAG 3 cut(s) 263, 1053, 1266
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 2 cut(s) 452, 701
BshFI GGCC 1 cut(s) 700
BsiEI CGRYCG 2 cut(s) 452, 701
BsiHKCI CYCGRG 1 cut(s) 850
BslI CCNNNNNNNGG 1 cut(s) 991
BsmAI GTCTC 8 cut(s) 404, 512, 677, 827, 962, 1097, 1223, 1326
BsnI GGCC 1 cut(s) 700
Bso31I GGTCTC 1 cut(s) 1326
BsoBI CYCGRG 1 cut(s) 850
Bsp143I GATC 4 cut(s) 50, 144, 166, 449
BspANI GGCC 1 cut(s) 700
BspCNI CTCAG 4 cut(s) 528, 543, 978, 1113
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 1 cut(s) 366
BspMI ACCTGC 3 cut(s) 285, 303, 1075
BspOI GCTAGC 1 cut(s) 1157
BspPI GGATC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 1326
BsrBI CCGCTC 3 cut(s) 352, 532, 1117
BsrI ACTGG 3 cut(s) 170, 491, 788
BssECI CCNNGG 7 cut(s) 638, 678, 923, 1058, 1224, 1336, 1337
BssMI GATC 4 cut(s) 50, 144, 166, 449
BssT1I CCWWGG 2 cut(s) 638, 1058
Bst2UI CCWGG 2 cut(s) 924, 1338
Bst4CI ACNGT 6 cut(s) 525, 690, 840, 975, 1110, 1236
BstAPI GCANNNNNTGC 1 cut(s) 790
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 9 cut(s) 420, 570, 585, 648, 735, 870, 1005, 1155, 1281
BstDEI CTNAG 8 cut(s) 536, 551, 716, 986, 1121, 1136, 1247, 1262
BstENI CCTNNNNNAGG 1 cut(s) 989
BstF5I GGATG 4 cut(s) 165, 247, 268, 1188
BstFNI CGCG 1 cut(s) 179
BstH2I RGCGCY 1 cut(s) 850
BstHHI GCGC 2 cut(s) 12, 849
BstKTI GATC 4 cut(s) 53, 147, 169, 452
BstMAI GTCTC 8 cut(s) 404, 512, 677, 827, 962, 1097, 1223, 1326
BstMBI GATC 4 cut(s) 50, 144, 166, 449
BstMCI CGRYCG 2 cut(s) 452, 701
BstNI CCWGG 2 cut(s) 924, 1338
BstNSI RCATGY 2 cut(s) 390, 572
BstSCI CCNGG 2 cut(s) 922, 1336
BstSNI TACGTA 1 cut(s) 100
BstUI CGCG 1 cut(s) 179
BstV2I GAAGAC 4 cut(s) 166, 819, 954, 1089
BstX2I RGATCY 2 cut(s) 50, 166
BstXI CCANNNNNNTGG 1 cut(s) 63
BstYI RGATCY 2 cut(s) 50, 166
BstZI CGGCCG 1 cut(s) 698
BsuRI GGCC 1 cut(s) 700
BtsCI GGATG 4 cut(s) 165, 247, 268, 1188
BtsIMutI CAGTG 2 cut(s) 548, 795
BveI ACCTGC 3 cut(s) 285, 303, 1075
Cac8I GCNNGC 9 cut(s) 420, 570, 585, 648, 735, 870, 1005, 1155, 1281
CaiI CAGNNNCTG 2 cut(s) 668, 1214
CfoI GCGC 2 cut(s) 12, 849
Cfr13I GGNCC 1 cut(s) 675
CseI GACGC 1 cut(s) 365
CviAII CATG 4 cut(s) 63, 387, 569, 1286
DdeI CTNAG 8 cut(s) 536, 551, 716, 986, 1121, 1136, 1247, 1262
DpnI GATC 4 cut(s) 52, 146, 168, 451
DpnII GATC 4 cut(s) 50, 144, 166, 449
EaeI YGGCCR 1 cut(s) 698
EagI CGGCCG 1 cut(s) 698
EclXI CGGCCG 1 cut(s) 698
Eco105I TACGTA 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 638, 1058
Eco31I GGTCTC 1 cut(s) 1326
Eco32I GATATC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 675
Eco47III AGCGCT 1 cut(s) 848
Eco52I CGGCCG 1 cut(s) 698
Eco57I CTGAAG 1 cut(s) 1083
Eco88I CYCGRG 1 cut(s) 850
EcoNI CCTNNNNNAGG 1 cut(s) 989
EcoO109I RGGNCCY 1 cut(s) 675
EcoRII CCWGG 2 cut(s) 922, 1336
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 638, 1058
EcoT22I ATGCAT 1 cut(s) 570
ErhI CCWWGG 2 cut(s) 638, 1058
FaeI CATG 4 cut(s) 66, 390, 572, 1289
FalI AAGNNNNNCTT 2 cut(s) 1290, 1322
FatI CATG 4 cut(s) 62, 386, 568, 1285
FauI CCCGC 3 cut(s) 345, 655, 940
FblI GTMKAC 2 cut(s) 591, 1161
FokI GGATG 4 cut(s) 172, 254, 255, 1195
GlaI GCGC 2 cut(s) 11, 848
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 850
HaeIII GGCC 1 cut(s) 700
HgaI GACGC 1 cut(s) 365
HhaI GCGC 2 cut(s) 12, 849
Hin1II CATG 4 cut(s) 66, 390, 572, 1289
Hin6I GCGC 2 cut(s) 10, 847
HinP1I GCGC 2 cut(s) 10, 847
HphI GGTGA 3 cut(s) 58, 284, 478
Hpy166II GTNNAC 2 cut(s) 592, 1162
Hpy188III TCNNGA 5 cut(s) 142, 380, 453, 501, 852
Hpy8I GTNNAC 2 cut(s) 592, 1162
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 6 cut(s) 525, 690, 840, 975, 1110, 1236
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 8 cut(s) 62, 185, 280, 298, 568, 572, 1070, 1283
HpyF3I CTNAG 8 cut(s) 536, 551, 716, 986, 1121, 1136, 1247, 1262
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 4 cut(s) 66, 390, 572, 1289
HspAI GCGC 2 cut(s) 10, 847
Kzo9I GATC 4 cut(s) 50, 144, 166, 449
LmnI GCTCC 1 cut(s) 364
LweI GCATC 4 cut(s) 277, 307, 555, 1266
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 393
MalI GATC 4 cut(s) 52, 146, 168, 451
MbiI CCGCTC 3 cut(s) 352, 532, 1117
MboI GATC 4 cut(s) 50, 144, 166, 449
MboII GAAGA 6 cut(s) 79, 166, 819, 954, 1042, 1089
MflI RGATCY 2 cut(s) 50, 166
MmeI TCCRAC 2 cut(s) 495, 594
Mph1103I ATGCAT 1 cut(s) 570
MslI CAYNNNNRTG 2 cut(s) 61, 158
MspA1I CMGCKG 2 cut(s) 29, 587
MspR9I CCNGG 2 cut(s) 924, 1338
MvaI CCWGG 2 cut(s) 924, 1338
MvnI CGCG 1 cut(s) 179
NdeII GATC 4 cut(s) 50, 144, 166, 449
NheI GCTAGC 1 cut(s) 1153
NlaIII CATG 4 cut(s) 66, 390, 572, 1289
NlaIV GGNNCC 1 cut(s) 366
NmeAIII GCCGAG 1 cut(s) 726
NmuCI GTSAC 1 cut(s) 393
NsiI ATGCAT 1 cut(s) 570
NspI RCATGY 2 cut(s) 390, 572
PaeI GCATGC 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 850
PaqCI CACCTGC 1 cut(s) 303
PasI CCCWGGG 1 cut(s) 1337
PfeI GAWTC 2 cut(s) 626, 1289
PflFI GACNNNGTC 1 cut(s) 523
Ple19I CGATCG 1 cut(s) 452
Ppu21I YACGTR 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 675
Psp6I CCWGG 2 cut(s) 922, 1336
PspGI CCWGG 2 cut(s) 922, 1336
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 675
PspPPI RGGWCCY 1 cut(s) 675
PstNI CAGNNNCTG 2 cut(s) 668, 1214
PsuI RGATCY 2 cut(s) 50, 166
PsyI GACNNNGTC 1 cut(s) 523
PvuI CGATCG 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 29
RseI CAYNNNNRTG 2 cut(s) 61, 158
Sau3AI GATC 4 cut(s) 50, 144, 166, 449
Sau96I GGNCC 1 cut(s) 675
ScrFI CCNGG 2 cut(s) 924, 1338
SfaNI GCATC 4 cut(s) 277, 307, 555, 1266
Sfr274I CTCGAG 1 cut(s) 850
SinI GGWCC 1 cut(s) 675
SlaI CTCGAG 1 cut(s) 850
SmiMI CAYNNNNRTG 2 cut(s) 61, 158
SmlI CTYRAG 2 cut(s) 850, 1307
SmoI CTYRAG 2 cut(s) 850, 1307
SnaBI TACGTA 1 cut(s) 100
SpeI ACTAGT 1 cut(s) 202
SphI GCATGC 1 cut(s) 572
StyD4I CCNGG 2 cut(s) 922, 1336
StyI CCWWGG 2 cut(s) 638, 1058
TaaI ACNGT 6 cut(s) 525, 690, 840, 975, 1110, 1236
TaiI ACGT 1 cut(s) 102
TaqI TCGA 2 cut(s) 452, 851
TauI GCSGC 5 cut(s) 10, 182, 535, 700, 1120
TfiI GAWTC 2 cut(s) 626, 1289
TscAI CASTG 2 cut(s) 555, 795
TseFI GTSAC 1 cut(s) 393
Tsp45I GTSAC 1 cut(s) 393
TspRI CASTG 2 cut(s) 555, 795
Tth111I GACNNNGTC 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 675
XagI CCTNNNNNAGG 1 cut(s) 989
XbaI TCTAGA 1 cut(s) 500
XceI RCATGY 2 cut(s) 390, 572
XhoI CTCGAG 1 cut(s) 850
XmiI GTMKAC 2 cut(s) 591, 1161
Zsp2I ATGCAT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.