FvH4_1g26220

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007246
Physical Location & Seq
Reverse (-)
18058107 .. 18062208
4102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g26220.t4

Sequence Viewer

Length: 1401 bp
ATGAGGAGGAGATCTTCTACTCTGCCTATGGTGGATGTGTTTGTGGTACTTGTTGCATTGTTCAGTGGTGCTCCGTCTCCATGGGCTGTTGTCAAGGGCAGTCATCCACATTGCAAAAAATTAAGGGAAAACTTGGGCGAAATTCGACCTCACAGTGTCACCATCACTGAATTTGGCGCAGTTGGAGACGGGGTCACCCTTAACACCAAAGCCTTTCAAAATGCCATCTTCTATCTCAACTCTTTCTCTGACAAGGGTGGGGCCAAGCTCTTTGTCCCTGCTGGCCGGTGGTTGACAGGAAGCTTCCATCTCATCAGTCATCTAACCTTGTGGTTGGATGAAGATGCAGTCATTCTTGGATCAACGAACTCAGATGATTGGCCAGTTGTTGATCCCTTGCCTTCATATGGTCGAGGAAGGGAGTTGATCGGGGAAAGACATCAAAGCCTCATCTATGGGCACAATTTGACCGATGTTATCATAACTGGTCATAATGGAACCGTTGATGGTCAAGGCAGCATCTGGTGGAATTGGTACCATAACAAAACTCTGAATTATACAAGGCCCCATTTGGTGGAGTTGATGAACTCAACTGCGGTTGTCATCTCAAACCTGACTTTCTTAAATTCACCATTTTGGACCATTCACCCAGTATACTGCAGCCATGTTTCCGTTCAGAATCTCACTATCCTTGCTCCTCTCAACTCACCAAACACAGATGGGATCGATCCAGATTCTTCTGATAATGTCTGCATTGAAGACTGCTATATCAGCACCGGTGATGATCTGATTTCCATCAAAAGTGGGTGGGATGAGTACGGCATTTCATATGGTCGTCCCAGTAGAAACATTCTCATTCGCGGGATTACAGGGAAATCTCAAACAAGTGCAGGAATATCAATTGGAAGTGAGATGTCCGGAGGTGTTTCAGAAGTTCATGCAGAAAATCTCCAATTTTTTGGTTCTCATACAGGTATCAGAATAAAGACATCTCCGGGAAGGGGTGGTTATGTCAGAAATATCTGTGTCTCAAACATGACCTTGGATGGTGTATATATAGCTATAAGGTTCACCAGTCAGTATGGGGAACACCCAGATGAGTTTTACGACCCAAGTGCCCTCCCTATCATAGAACATATTACTTTTAAAGATGTCTTAGGTGATAATATCAAAATTGCAGGCCTCCTAGAGGGTTTGGAAGGTGGCAGTTTTCAGAACATTTGCCTATCCAATATATTCCTCAATGTAACATCAAAGTCCCCATGGAACTGCTCTTCTATTCATGGATATTCTTACTTAGTTTCTCCAGAAATATGTGAGCCTCTGAAGGAGAGTATCTCCCCTGAGAAATACTCCGATTGTTACCATCTATCAGATCCGAAACGGTTTTCCAGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

467

Amino Acids

51.3

Weight (kDa)

5.74

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 45 - 325 8.2e-42 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 534
AccB1I GGYRCC 1 cut(s) 534
AccB7I CCANNNNNTGG 1 cut(s) 574
AccI GTMKAC 1 cut(s) 654
AccII CGCG 1 cut(s) 861
AccIII TCCGGA 1 cut(s) 917
AciI CCGC 2 cut(s) 596, 861
AclWI GGATC 5 cut(s) 367, 386, 722, 731, 1370
AcoI YGGCCR 2 cut(s) 283, 380
AcsI RAATTY 3 cut(s) 141, 170, 625
AcuI CTGAAG 1 cut(s) 1346
AfaI GTAC 3 cut(s) 48, 536, 818
AfiI CCNNNNNNNGG 4 cut(s) 407, 574, 1001, 1189
AgeI ACCGGT 1 cut(s) 776
AgsI TTSAA 2 cut(s) 218, 758
AluBI AGCT 3 cut(s) 268, 303, 1061
AluI AGCT 3 cut(s) 268, 303, 1061
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 3 cut(s) 81, 180, 1033
AlwI GGATC 5 cut(s) 367, 386, 722, 731, 1370
AlwNI CAGNNNCTG 1 cut(s) 522
Aor13HI TCCGGA 1 cut(s) 917
AoxI GGCC 5 cut(s) 261, 283, 380, 563, 1180
ApeKI GCWGC 2 cut(s) 516, 660
ApoI RAATTY 3 cut(s) 141, 170, 625
AsiGI ACCGGT 1 cut(s) 776
Asp718I GGTACC 1 cut(s) 534
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 3 cut(s) 261, 564, 639
AsuC2I CCSGG 1 cut(s) 996
AsuHPI GGTGA 8 cut(s) 151, 187, 621, 638, 699, 791, 1063, 1172
AvaII GGWCC 1 cut(s) 639
BaeGI GKGCMC 2 cut(s) 462, 1120
BalI TGGCCA 1 cut(s) 382
BanI GGYRCC 1 cut(s) 534
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 528, 672
BccI CCATC 8 cut(s) 170, 233, 315, 500, 713, 803, 1040, 1374
BceAI ACGGC 1 cut(s) 835
BcgI CGANNNNNNTGC 2 cut(s) 1097, 1131
BcnI CCSGG 1 cut(s) 996
BcoDI GTCTC 3 cut(s) 81, 180, 1033
BfaI CTAG 1 cut(s) 1187
BfmI CTRYAG 1 cut(s) 658
BglII AGATCT 1 cut(s) 11
BisI GCNGC 2 cut(s) 517, 661
BlsI GCNGC 2 cut(s) 518, 662
Bme1390I CCNGG 1 cut(s) 996
Bme18I GGWCC 1 cut(s) 639
BmgT120I GGNCC 3 cut(s) 261, 564, 639
BmiI GGNNCC 4 cut(s) 262, 499, 536, 566
BmrFI CCNGG 1 cut(s) 996
BmrI ACTGGG 2 cut(s) 644, 834
BmsI GCATC 2 cut(s) 334, 528
BmuI ACTGGG 2 cut(s) 644, 834
BpiI GAAGAC 1 cut(s) 765
BplI GAGNNNNNCTC 4 cut(s) 1322, 1354, 1337, 1369
BpmI CTGGAG 1 cut(s) 1290
BpuMI CCSGG 1 cut(s) 996
Bsa29I ATCGAT 1 cut(s) 726
BsaBI GATNNNNATC 1 cut(s) 794
BsaJI CCNNGG 3 cut(s) 80, 1041, 1262
BsaWI WCCGGW 2 cut(s) 776, 917
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 574, 1001, 1189
Bse118I RCCGGY 2 cut(s) 285, 776
Bse1I ACTGG 6 cut(s) 383, 490, 650, 840, 1074, 1392
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 794
BseAI TCCGGA 1 cut(s) 917
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 3 cut(s) 80, 1041, 1262
BseGI GGATG 5 cut(s) 40, 103, 343, 817, 1051
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 4 cut(s) 407, 574, 1001, 1189
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 384, 1335
BseNI ACTGG 6 cut(s) 383, 490, 650, 840, 1074, 1392
BseRI GAGGAG 3 cut(s) 19, 22, 687
BseSI GKGCMC 2 cut(s) 462, 1120
BseXI GCAGC 2 cut(s) 528, 672
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 1 cut(s) 861
BshFI GGCC 5 cut(s) 263, 285, 382, 565, 1182
BshNI GGYRCC 1 cut(s) 534
BshTI ACCGGT 1 cut(s) 776
BshVI ATCGAT 1 cut(s) 726
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 4 cut(s) 286, 777, 918, 995
BslFI GGGAC 3 cut(s) 260, 822, 1243
BslI CCNNNNNNNGG 4 cut(s) 407, 574, 1001, 1189
BsmAI GTCTC 3 cut(s) 81, 180, 1033
BsmBI CGTCTC 2 cut(s) 81, 180
BsmFI GGGAC 3 cut(s) 260, 822, 1243
BsnI GGCC 5 cut(s) 263, 285, 382, 565, 1182
Bsp1286I GDGCHC 3 cut(s) 73, 462, 1120
Bsp13I TCCGGA 1 cut(s) 917
Bsp143I GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
Bsp19I CCATGG 2 cut(s) 80, 1262
BspACI CCGC 2 cut(s) 596, 861
BspANI GGCC 5 cut(s) 263, 285, 382, 565, 1182
BspCNI CTCAG 2 cut(s) 383, 1336
BspDI ATCGAT 1 cut(s) 726
BspEI TCCGGA 1 cut(s) 917
BspFNI CGCG 1 cut(s) 861
BspLI GGNNCC 4 cut(s) 262, 499, 536, 566
BspMAI CTGCAG 1 cut(s) 662
BspPI GGATC 5 cut(s) 367, 386, 722, 731, 1370
BspQI GCTCTTC 1 cut(s) 1279
BspT107I GGYRCC 1 cut(s) 534
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 2 cut(s) 285, 776
BsrI ACTGG 6 cut(s) 383, 490, 650, 840, 1074, 1392
BssAI RCCGGY 2 cut(s) 285, 776
BssECI CCNNGG 3 cut(s) 80, 1041, 1262
BssMI GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
BssNAI GTATAC 1 cut(s) 655
BssT1I CCWWGG 3 cut(s) 80, 1041, 1262
Bst1107I GTATAC 1 cut(s) 655
Bst4CI ACNGT 3 cut(s) 155, 502, 1386
Bst6I CTCTTC 1 cut(s) 1279
BstC8I GCNNGC 2 cut(s) 283, 1180
BstDEI CTNAG 4 cut(s) 370, 1156, 1297, 1344
BstDSI CCRYGG 2 cut(s) 80, 1262
BstEII GGTNACC 1 cut(s) 193
BstENI CCTNNNNNAGG 1 cut(s) 1187
BstF5I GGATG 5 cut(s) 40, 103, 343, 817, 1051
BstFNI CGCG 1 cut(s) 861
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 8 cut(s) 14, 362, 394, 429, 726, 730, 787, 1378
BstMAI GTCTC 3 cut(s) 81, 180, 1033
BstMBI GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
BstMWI GCNNNNNNNGC 1 cut(s) 771
BstPI GGTNACC 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 994
BstSFI CTRYAG 1 cut(s) 658
BstSLI GKGCMC 2 cut(s) 462, 1120
BstUI CGCG 1 cut(s) 861
BstV1I GCAGC 2 cut(s) 528, 672
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 2 cut(s) 11, 1375
BstXI CCANNNNNNTGG 1 cut(s) 959
BstYI RGATCY 2 cut(s) 11, 1375
BstZ17I GTATAC 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 726
BsuRI GGCC 5 cut(s) 263, 285, 382, 565, 1182
BsuTUI ATCGAT 1 cut(s) 726
BtgI CCRYGG 2 cut(s) 80, 1262
BtsCI GGATG 5 cut(s) 40, 103, 343, 817, 1051
BtsIMutI CAGTG 4 cut(s) 70, 160, 165, 1399
Cac8I GCNNGC 2 cut(s) 283, 1180
CaiI CAGNNNCTG 1 cut(s) 522
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 2 cut(s) 285, 776
Cfr13I GGNCC 3 cut(s) 261, 564, 639
ClaI ATCGAT 1 cut(s) 726
Csp6I GTAC 3 cut(s) 47, 535, 817
CspAI ACCGGT 1 cut(s) 776
CviAII CATG 6 cut(s) 81, 665, 938, 1036, 1263, 1283
CviQI GTAC 3 cut(s) 47, 535, 817
DdeI CTNAG 4 cut(s) 370, 1156, 1297, 1344
DpnI GATC 8 cut(s) 13, 361, 393, 428, 725, 729, 786, 1377
DpnII GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
DraI TTTAAA 1 cut(s) 1147
EaeI YGGCCR 2 cut(s) 283, 380
Eam1104I CTCTTC 1 cut(s) 1279
EarI CTCTTC 1 cut(s) 1279
Eco130I CCWWGG 3 cut(s) 80, 1041, 1262
Eco147I AGGCCT 1 cut(s) 1182
Eco47I GGWCC 1 cut(s) 639
Eco57I CTGAAG 1 cut(s) 1346
Eco91I GGTNACC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 1187
EcoO109I RGGNCCY 1 cut(s) 564
EcoO65I GGTNACC 1 cut(s) 193
EcoT14I CCWWGG 3 cut(s) 80, 1041, 1262
ErhI CCWWGG 3 cut(s) 80, 1041, 1262
Esp3I CGTCTC 2 cut(s) 81, 180
FaeI CATG 6 cut(s) 84, 668, 941, 1039, 1266, 1286
FaqI GGGAC 3 cut(s) 260, 822, 1243
FatI CATG 6 cut(s) 80, 664, 937, 1035, 1262, 1282
FauI CCCGC 1 cut(s) 854
FauNDI CATATG 2 cut(s) 406, 829
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 2 cut(s) 517, 661
FokI GGATG 5 cut(s) 47, 90, 350, 824, 1058
Fsp4HI GCNGC 2 cut(s) 517, 661
FspBI CTAG 1 cut(s) 1187
GlaI GCGC 1 cut(s) 178
GluI GCNGC 2 cut(s) 517, 661
GsuI CTGGAG 1 cut(s) 1290
HaeIII GGCC 5 cut(s) 263, 285, 382, 565, 1182
HapII CCGG 4 cut(s) 286, 777, 918, 995
HhaI GCGC 1 cut(s) 179
Hin1II CATG 6 cut(s) 84, 668, 941, 1039, 1266, 1286
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 2 cut(s) 679, 734
HpaII CCGG 4 cut(s) 286, 777, 918, 995
HphI GGTGA 8 cut(s) 151, 187, 621, 638, 699, 791, 1063, 1172
Hpy166II GTNNAC 3 cut(s) 294, 655, 1071
Hpy188III TCNNGA 3 cut(s) 731, 918, 1307
Hpy8I GTNNAC 3 cut(s) 294, 655, 1071
HpyAV CCTTC 5 cut(s) 411, 411, 993, 1193, 1321
HpyCH4III ACNGT 3 cut(s) 155, 502, 1386
HpyCH4V TGCA 8 cut(s) 56, 114, 347, 660, 753, 890, 941, 1178
HpyF10VI GCNNNNNNNGC 1 cut(s) 771
HpyF3I CTNAG 4 cut(s) 370, 1156, 1297, 1344
Hsp92II CATG 6 cut(s) 84, 668, 941, 1039, 1266, 1286
HspAI GCGC 1 cut(s) 177
Kpn2I TCCGGA 1 cut(s) 917
KpnI GGTACC 1 cut(s) 538
Kzo9I GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
LguI GCTCTTC 1 cut(s) 1279
LmnI GCTCC 2 cut(s) 76, 700
Lsp1109I GCAGC 2 cut(s) 528, 672
LweI GCATC 2 cut(s) 334, 528
MaeI CTAG 1 cut(s) 1187
MaeIII GTNAC 4 cut(s) 157, 193, 1246, 1361
MalI GATC 8 cut(s) 13, 361, 393, 428, 725, 729, 786, 1377
MboI GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
MboII GAAGA 6 cut(s) 6, 220, 353, 729, 770, 1266
MfeI CAATTG 1 cut(s) 900
MflI RGATCY 2 cut(s) 11, 1375
MhlI GDGCHC 3 cut(s) 73, 462, 1120
MlsI TGGCCA 1 cut(s) 382
MluNI TGGCCA 1 cut(s) 382
MmeI TCCRAC 2 cut(s) 163, 315
Mox20I TGGCCA 1 cut(s) 382
MroI TCCGGA 1 cut(s) 917
MscI TGGCCA 1 cut(s) 382
MseI TTAA 4 cut(s) 122, 201, 623, 1146
MslI CAYNNNNRTG 1 cut(s) 1095
Msp20I TGGCCA 1 cut(s) 382
MspI CCGG 4 cut(s) 286, 777, 918, 995
MspR9I CCNGG 1 cut(s) 996
MunI CAATTG 1 cut(s) 900
MvnI CGCG 1 cut(s) 861
MwoI GCNNNNNNNGC 1 cut(s) 771
NciI CCSGG 1 cut(s) 996
NcoI CCATGG 2 cut(s) 80, 1262
NdeI CATATG 2 cut(s) 406, 829
NdeII GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
NlaIII CATG 6 cut(s) 84, 668, 941, 1039, 1266, 1286
NlaIV GGNNCC 4 cut(s) 262, 499, 536, 566
NmuCI GTSAC 2 cut(s) 157, 193
PceI AGGCCT 1 cut(s) 1182
PciSI GCTCTTC 1 cut(s) 1279
PfeI GAWTC 2 cut(s) 679, 734
PflFI GACNNNGTC 1 cut(s) 191
PflMI CCANNNNNTGG 1 cut(s) 574
PfoI TCCNGGA 1 cut(s) 994
PinAI ACCGGT 1 cut(s) 776
PkrI GCNGC 2 cut(s) 518, 662
PspEI GGTNACC 1 cut(s) 193
PspN4I GGNNCC 4 cut(s) 262, 499, 536, 566
PspPI GGNCC 3 cut(s) 261, 564, 639
PstI CTGCAG 1 cut(s) 662
PstNI CAGNNNCTG 1 cut(s) 522
PsuI RGATCY 2 cut(s) 11, 1375
PsyI GACNNNGTC 1 cut(s) 191
RsaI GTAC 3 cut(s) 48, 536, 818
RsaNI GTAC 3 cut(s) 47, 535, 817
RseI CAYNNNNRTG 1 cut(s) 1095
SapI GCTCTTC 1 cut(s) 1279
SaqAI TTAA 4 cut(s) 122, 201, 623, 1146
SatI GCNGC 2 cut(s) 517, 661
Sau3AI GATC 8 cut(s) 11, 359, 391, 426, 723, 727, 784, 1375
Sau96I GGNCC 3 cut(s) 261, 564, 639
ScrFI CCNGG 1 cut(s) 996
SduI GDGCHC 3 cut(s) 73, 462, 1120
SfaNI GCATC 2 cut(s) 334, 528
SfcI CTRYAG 1 cut(s) 658
SgrAI CRCCGGYG 1 cut(s) 776
SinI GGWCC 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 1095
SseBI AGGCCT 1 cut(s) 1182
SsiI CCGC 2 cut(s) 596, 861
SspMI CTAG 1 cut(s) 1187
StuI AGGCCT 1 cut(s) 1182
StyD4I CCNGG 1 cut(s) 994
StyI CCWWGG 3 cut(s) 80, 1041, 1262
TaaI ACNGT 3 cut(s) 155, 502, 1386
TaqI TCGA 3 cut(s) 145, 412, 726
TaqII GACCGA 1 cut(s) 485
TfiI GAWTC 2 cut(s) 679, 734
Tru1I TTAA 4 cut(s) 122, 201, 623, 1146
Tru9I TTAA 4 cut(s) 122, 201, 623, 1146
TscAI CASTG 4 cut(s) 70, 160, 172, 1399
TseFI GTSAC 2 cut(s) 157, 193
TseI GCWGC 2 cut(s) 516, 660
Tsp45I GTSAC 2 cut(s) 157, 193
TspDTI ATGAA 6 cut(s) 354, 393, 599, 816, 926, 1271
TspGWI ACGGA 2 cut(s) 63, 661
TspRI CASTG 4 cut(s) 70, 160, 172, 1399
Tth111I GACNNNGTC 1 cut(s) 191
Van91I CCANNNNNTGG 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 639
XagI CCTNNNNNAGG 1 cut(s) 1187
XapI RAATTY 3 cut(s) 141, 170, 625
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.