Rh3CG304400

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0007246
Physical Location & Seq
Reverse (-)
31722589 .. 31726816
4228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG304400.1

Sequence Viewer

Length: 990 bp
ATGATGAGGAATTGTCTCCACATGTGCATTTGGCTTTATAAATTGATGTTGCTGACTTGCTGTTCTCTTCATGCAGGTCATAATGGAACGATTGATGGTCAAGGCAGCATTTGGTGGAATTCATTCCGTAACAAAACTCTGAATTATACAAGGCCCCATTTGGTGGAGTTGATGAGCTCAACTGGGGTTGTCATCTCAAACCTGACTTTCTTAAATTCACCATTTTGGACCATTCACCCAGTATACTGCAGCCATGTTTCCGTCCAGAATCTCACAATCCTTGCTCCTCTCAATTCACCAAACACGGATGGGATCGATCCAGACTCTTCTGATAATGTCTGCATTGAAGACTGTTATATAAGCACTGGTGATGATCTGATCTCCATCAAAAGTGGTTGGGATGAGTATGGCATTTCATATGGTCGCCCCAGTAGAAACATTCTCATTCGCGGGATTACGGGGAAAACTCAAACAAGTGCAGGAATTTCAATTGGAAGTGAGATGTCTGGAGGTGTTTCAGAAGTTCATGCAGAAAATCTCCATTTTTTCGGTTCTCATACGGGTATCAGAATAAAGACATCTCCGGGAAGGGGTGGTTATGTCAGAAATATCTCTGTGTCAAATATGACCTTGGATGGTGTATATATAGCTATAAGGTTCACCAGTCACTATGGAGAACACCCAGATGAGTTTTTCGACCCAAGTGCCCTCCCTATCGTAGAACAGATTACTTTTAAAGATGTCATAGGCGATAATATCAAAACTGCAGGCCTCCTAGAGGGTTTAGAAGGTGGCAGTTTTCAGAACATTTGCCTATCCAATATTATCCTCAATGTAACATCAAAGTCCCCATGGAACTGCTCTTCTATTCATGGATATTCTTACTTTGTTTCTCCAGAAATCTGTCAGCCTCTGAAGGAGAGTATCTCCCCTGAGCATTACTCGGATTGTTACCATCTATCAGATCAGAAATGGTTTTCCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

329

Amino Acids

36.36

Weight (kDa)

5.72

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 27 - 291 3.8e-42 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 39
Acc36I ACCTGC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 163
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 450
AciI CCGC 1 cut(s) 450
AclWI GGATC 2 cut(s) 311, 320
AcsI RAATTY 3 cut(s) 118, 214, 483
AcuI CTGAAG 1 cut(s) 935
AfiI CCNNNNNNNGG 3 cut(s) 163, 590, 778
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 2 cut(s) 347, 489
AluBI AGCT 2 cut(s) 177, 650
AluI AGCT 2 cut(s) 177, 650
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 2 cut(s) 311, 320
AlwNI CAGNNNCTG 1 cut(s) 913
AoxI GGCC 2 cut(s) 152, 769
ApeKI GCWGC 2 cut(s) 105, 249
ApoI RAATTY 3 cut(s) 118, 214, 483
Asp700I GAANNNNTTC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 153, 228
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 5 cut(s) 210, 227, 288, 380, 652
AvaII GGWCC 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 709
BanII GRGCYC 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 2 cut(s) 117, 261
BccI CCATC 5 cut(s) 89, 302, 392, 629, 963
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 2 cut(s) 247, 765
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 2 cut(s) 106, 250
BlsI GCNGC 2 cut(s) 107, 251
Bme1390I CCNGG 1 cut(s) 585
Bme18I GGWCC 1 cut(s) 228
BmgT120I GGNCC 2 cut(s) 153, 228
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 1 cut(s) 585
BmrI ACTGGG 3 cut(s) 192, 233, 423
BmuI ACTGGG 3 cut(s) 192, 233, 423
BpiI GAAGAC 1 cut(s) 354
BplI GAGNNNNNCTC 4 cut(s) 911, 943, 926, 958
BpmI CTGGAG 2 cut(s) 528, 879
Bpu10I CCTNAGC 1 cut(s) 933
BpuMI CCSGG 1 cut(s) 585
Bsa29I ATCGAT 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 630, 851
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 590, 778
Bse1I ACTGG 6 cut(s) 187, 239, 370, 429, 663, 981
Bse8I GATNNNNATC 1 cut(s) 383
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 630, 851
BseGI GGATG 3 cut(s) 313, 406, 640
BseJI GATNNNNATC 1 cut(s) 383
BseLI CCNNNNNNNGG 3 cut(s) 163, 590, 778
BseMII CTCAG 1 cut(s) 924
BseNI ACTGG 6 cut(s) 187, 239, 370, 429, 663, 981
BseRI GAGGAG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 709
BseXI GCAGC 2 cut(s) 117, 261
BsgI GTGCAG 1 cut(s) 498
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 2 cut(s) 154, 771
BshVI ATCGAT 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 1 cut(s) 584
BslFI GGGAC 1 cut(s) 832
BslI CCNNNNNNNGG 3 cut(s) 163, 590, 778
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 832
BsnI GGCC 2 cut(s) 154, 771
Bsp1286I GDGCHC 2 cut(s) 179, 709
Bsp143I GATC 5 cut(s) 312, 316, 373, 378, 964
Bsp19I CCATGG 1 cut(s) 851
BspACI CCGC 1 cut(s) 450
BspANI GGCC 2 cut(s) 154, 771
BspCNI CTCAG 1 cut(s) 925
BspDI ATCGAT 1 cut(s) 315
BspFNI CGCG 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 155
BspMAI CTGCAG 2 cut(s) 251, 769
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 2 cut(s) 311, 320
BspQI GCTCTTC 1 cut(s) 868
BsrI ACTGG 6 cut(s) 187, 239, 370, 429, 663, 981
BssECI CCNNGG 2 cut(s) 630, 851
BssMI GATC 5 cut(s) 312, 316, 373, 378, 964
BssNAI GTATAC 1 cut(s) 244
BssT1I CCWWGG 2 cut(s) 630, 851
Bst1107I GTATAC 1 cut(s) 244
Bst4CI ACNGT 1 cut(s) 353
Bst6I CTCTTC 3 cut(s) 72, 331, 868
BstC8I GCNNGC 1 cut(s) 769
BstDEI CTNAG 1 cut(s) 933
BstDSI CCRYGG 1 cut(s) 851
BstENI CCTNNNNNAGG 1 cut(s) 776
BstF5I GGATG 3 cut(s) 313, 406, 640
BstFNI CGCG 1 cut(s) 450
BstKTI GATC 5 cut(s) 315, 319, 376, 381, 967
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 5 cut(s) 312, 316, 373, 378, 964
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 583
BstSFI CTRYAG 2 cut(s) 247, 765
BstSLI GKGCMC 1 cut(s) 709
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 2 cut(s) 117, 261
BstV2I GAAGAC 1 cut(s) 354
BstZ17I GTATAC 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 2 cut(s) 154, 771
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 851
BtsCI GGATG 3 cut(s) 313, 406, 640
BtsIMutI CAGTG 2 cut(s) 363, 988
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 769
CaiI CAGNNNCTG 1 cut(s) 913
Cfr13I GGNCC 2 cut(s) 153, 228
ClaI ATCGAT 1 cut(s) 315
CviAII CATG 6 cut(s) 22, 71, 254, 527, 852, 872
CviJI RGCY 7 cut(s) 34, 154, 177, 252, 650, 771, 910
CviKI_1 RGCY 7 cut(s) 34, 154, 177, 252, 650, 771, 910
DdeI CTNAG 1 cut(s) 933
DpnI GATC 5 cut(s) 314, 318, 375, 380, 966
DpnII GATC 5 cut(s) 312, 316, 373, 378, 964
DraI TTTAAA 1 cut(s) 736
Eam1104I CTCTTC 3 cut(s) 72, 331, 868
EarI CTCTTC 3 cut(s) 72, 331, 868
Ecl136II GAGCTC 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 630, 851
Eco147I AGGCCT 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 935
EcoICRI GAGCTC 1 cut(s) 177
EcoNI CCTNNNNNAGG 1 cut(s) 776
EcoO109I RGGNCCY 1 cut(s) 153
EcoRI GAATTC 1 cut(s) 118
EcoT14I CCWWGG 2 cut(s) 630, 851
EcoT38I GRGCYC 1 cut(s) 179
ErhI CCWWGG 2 cut(s) 630, 851
FaeI CATG 6 cut(s) 25, 74, 257, 530, 855, 875
FaqI GGGAC 1 cut(s) 832
FatI CATG 6 cut(s) 21, 70, 253, 526, 851, 871
FauI CCCGC 1 cut(s) 443
FauNDI CATATG 1 cut(s) 418
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 106, 250
FokI GGATG 3 cut(s) 320, 413, 647
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 2 cut(s) 106, 250
FspBI CTAG 1 cut(s) 776
GluI GCNGC 2 cut(s) 106, 250
GsuI CTGGAG 2 cut(s) 528, 879
HaeIII GGCC 2 cut(s) 154, 771
HapII CCGG 1 cut(s) 584
Hin1II CATG 6 cut(s) 25, 74, 257, 530, 855, 875
HinfI GANTC 2 cut(s) 268, 323
HpaII CCGG 1 cut(s) 584
HphI GGTGA 5 cut(s) 210, 227, 288, 380, 652
Hpy166II GTNNAC 2 cut(s) 244, 660
Hpy188III TCNNGA 4 cut(s) 265, 320, 507, 896
Hpy8I GTNNAC 2 cut(s) 244, 660
HpyAV CCTTC 3 cut(s) 582, 782, 910
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4V TGCA 8 cut(s) 27, 74, 249, 342, 479, 530, 767, 986
HpyF3I CTNAG 1 cut(s) 933
Hsp92II CATG 6 cut(s) 25, 74, 257, 530, 855, 875
Kzo9I GATC 5 cut(s) 312, 316, 373, 378, 964
LguI GCTCTTC 1 cut(s) 868
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 2 cut(s) 117, 261
MaeI CTAG 1 cut(s) 776
MaeIII GTNAC 4 cut(s) 128, 665, 835, 950
MalI GATC 5 cut(s) 314, 318, 375, 380, 966
MboI GATC 5 cut(s) 312, 316, 373, 378, 964
MboII GAAGA 4 cut(s) 59, 318, 359, 855
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 2 cut(s) 179, 709
MluCI AATT 8 cut(s) 10, 41, 118, 142, 214, 292, 483, 489
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 7 cut(s) 297, 503, 719, 772, 782, 839, 921
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 2 cut(s) 212, 735
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 1 cut(s) 585
MunI CAATTG 1 cut(s) 489
MvnI CGCG 1 cut(s) 450
NciI CCSGG 1 cut(s) 585
NcoI CCATGG 1 cut(s) 851
NdeI CATATG 1 cut(s) 418
NdeII GATC 5 cut(s) 312, 316, 373, 378, 964
NlaIII CATG 6 cut(s) 25, 74, 257, 530, 855, 875
NlaIV GGNNCC 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 665
NspI RCATGY 1 cut(s) 25
PceI AGGCCT 1 cut(s) 771
PciI ACATGT 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 868
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 268
PflMI CCANNNNNTGG 1 cut(s) 163
PfoI TCCNGGA 1 cut(s) 583
PkrI GCNGC 2 cut(s) 107, 251
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PscI ACATGT 1 cut(s) 21
PsiI TTATAA 1 cut(s) 39
Psp124BI GAGCTC 1 cut(s) 179
PspN4I GGNNCC 1 cut(s) 155
PspPI GGNCC 2 cut(s) 153, 228
PstI CTGCAG 2 cut(s) 251, 769
PstNI CAGNNNCTG 1 cut(s) 913
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 868
SaqAI TTAA 2 cut(s) 212, 735
SatI GCNGC 2 cut(s) 106, 250
Sau3AI GATC 5 cut(s) 312, 316, 373, 378, 964
Sau96I GGNCC 2 cut(s) 153, 228
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 585
SduI GDGCHC 2 cut(s) 179, 709
SetI ASST 8 cut(s) 79, 179, 204, 514, 632, 652, 659, 793
SfcI CTRYAG 2 cut(s) 247, 765
SinI GGWCC 1 cut(s) 228
SmiMI CAYNNNNRTG 1 cut(s) 684
Sse9I AATT 8 cut(s) 10, 41, 118, 142, 214, 292, 483, 489
SseBI AGGCCT 1 cut(s) 771
SsiI CCGC 1 cut(s) 450
SspI AATATT 1 cut(s) 823
SspMI CTAG 1 cut(s) 776
SstI GAGCTC 1 cut(s) 179
StuI AGGCCT 1 cut(s) 771
StyD4I CCNGG 1 cut(s) 583
StyI CCWWGG 2 cut(s) 630, 851
TaaI ACNGT 1 cut(s) 353
TaqI TCGA 2 cut(s) 315, 696
TasI AATT 8 cut(s) 10, 41, 118, 142, 214, 292, 483, 489
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 2 cut(s) 212, 735
Tru9I TTAA 2 cut(s) 212, 735
TscAI CASTG 2 cut(s) 370, 988
TseFI GTSAC 1 cut(s) 665
TseI GCWGC 2 cut(s) 105, 249
Tsp45I GTSAC 1 cut(s) 665
TspDTI ATGAA 5 cut(s) 59, 111, 405, 515, 860
TspGWI ACGGA 3 cut(s) 116, 250, 320
TspRI CASTG 2 cut(s) 370, 988
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 228
XagI CCTNNNNNAGG 1 cut(s) 776
XapI RAATTY 3 cut(s) 118, 214, 483
XceI RCATGY 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.