RchiOBHm_Chr3g0485761

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007246
Physical Location & Seq
Reverse (-)
32564941 .. 32568961
4021 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45034

Sequence Viewer

Length: 1398 bp
ATGAGGAGGAGATCTTCTACTCTGCCTGTGGTGGATGTGTTTGTGGTACTTGTTGCATTGTTCACCGGTGCTCCGTTTCCATGGGCTGCTGTCAAGGGCAGTCATCCACATTGCAAGAAATTAAGGGAAAACTCGGGCATTCGACCTCACAGTGTCACCATCACTGAATTTGGCGCAGTTGGTGATGGGGTCACTCTCAACACCAAAGCCTTTCAGAATGCCATCTTCTATCTCAACTCTTTTTCTGACAAGGGTGGGGCCAAGCTTTTTGTCCCCGCTGGCCGGTGGTTGACAGGAAGCTTCCATCTCATCAGTCATCTAACCTTGTGGTTGGATGAAGATGCAGTCATTCTTGGATCAATGAACTCAGATGATTGGCCAGTTGTTGATCCCTTGCCATCATATGGTCGAGGAAGGGAGTTGATGGGGGAAAGACATCAAAGCCTCATCTATGGGCACAATTTGACCGATGTTATCATAACTGGTCATAATGGAACGATTGATGGTCAAGGCAGCATTTGGTGGAATTCGTTCCGTAACAAAACTCTGAATTATACAAGGCCCCATTTGGTGGAGTTGATGAACTCAACTGGGGTTGTCATCTCAAACCTGACTTTCTTAAATTCACCATTTTGGACCATTCACCCAGTATACTGCAGCCATGTTTCCGTCCAGAATCTCACAATCCTTGCTCCTCTCAATTCACCAAACACGGATGGCATCGATCCAGACTCTTCTGATAATGTCTGCATTGAAGACTGTTATATAAGCACCGGTGATGATCTGATCTCCATCAAAAGTGGGTGGGATGAGTATGGCATTTCATATGGTCGTCCCAGTAGAAACATTCTCATTCGCGGGATTACGGGGAAAACTCAAACAAGTGCAGGAATTTCAATTGGAAGTGAGATGTCTGGAGGTGTTTCAGAAGTTCATGCAGAAAATCTCCATTTTTTCGGTTCTCATACAGGTATCAGAATAAAGACATCTCCGGGAAGGGGTGGTTATGTCAGAAATATCTCTGTGTCAAATATGACCTTGGATGGTGTATATATAGCTATAAGGTTCACCAGTCACTATGGAGAACACCCAGATGAGTTTTTTGACCCAAGTGCCCTCCCTATCGTAGAACAGATTACTTTTAAAGATGTCATAGGCGATAATATCAAAACTGCAGGCCTCCTAGAGGGTTTAGAAGGTGGCAGTTTTCAGAACATTTGCCTATCCAATATTATCCTCAATGTAACATCAAAGTCCCCATGGAACTGCTCTTCTATTCATGGATATTCTTACTTTGTTTCTCCAGAAATCTGTCAGCCTCTGAAGGAGAGTATCTCCCCTGAGCATTACTCGGATTGTTACCATCTATCAGATCAGAAATGGTTTTCCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

465

Amino Acids

51.09

Weight (kDa)

5.92

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 147 - 427 3.6e-43 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 404, 571
AccI GTMKAC 1 cut(s) 651
AccII CGCG 1 cut(s) 858
AciI CCGC 2 cut(s) 276, 858
AclWI GGATC 3 cut(s) 364, 383, 719
AcoI YGGCCR 2 cut(s) 280, 377
AcsI RAATTY 4 cut(s) 167, 526, 622, 891
AcuI CTGAAG 1 cut(s) 1343
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 5 cut(s) 282, 404, 571, 998, 1186
AgeI ACCGGT 2 cut(s) 65, 773
AgsI TTSAA 2 cut(s) 755, 897
AluBI AGCT 3 cut(s) 265, 300, 1058
AluI AGCT 3 cut(s) 265, 300, 1058
Alw21I GWGCWC 1 cut(s) 73
AlwI GGATC 3 cut(s) 364, 383, 719
AlwNI CAGNNNCTG 1 cut(s) 1321
Ama87I CYCGRG 1 cut(s) 133
AoxI GGCC 5 cut(s) 258, 280, 377, 560, 1177
ApeKI GCWGC 3 cut(s) 86, 513, 657
ApoI RAATTY 4 cut(s) 167, 526, 622, 891
AsiGI ACCGGT 2 cut(s) 65, 773
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 258, 561, 636
AsuC2I CCSGG 1 cut(s) 993
AsuHPI GGTGA 8 cut(s) 55, 148, 194, 618, 635, 696, 788, 1060
AvaI CYCGRG 1 cut(s) 133
AvaII GGWCC 1 cut(s) 636
BaeGI GKGCMC 2 cut(s) 459, 1117
BalI TGGCCA 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 762
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 73, 525, 669
BcnI CCSGG 1 cut(s) 993
BfaI CTAG 1 cut(s) 1184
BfmI CTRYAG 2 cut(s) 655, 1173
BglII AGATCT 1 cut(s) 11
BisI GCNGC 3 cut(s) 87, 514, 658
BlsI GCNGC 3 cut(s) 88, 515, 659
Bme1390I CCNGG 1 cut(s) 993
Bme18I GGWCC 1 cut(s) 636
BmeT110I CYCGRG 1 cut(s) 133
BmgT120I GGNCC 3 cut(s) 258, 561, 636
BmiI GGNNCC 2 cut(s) 259, 563
BmrFI CCNGG 1 cut(s) 993
BmrI ACTGGG 3 cut(s) 600, 641, 831
BmsI GCATC 2 cut(s) 331, 729
BmuI ACTGGG 3 cut(s) 600, 641, 831
BpiI GAAGAC 1 cut(s) 762
BplI GAGNNNNNCTC 4 cut(s) 1319, 1351, 1334, 1366
BpmI CTGGAG 2 cut(s) 936, 1287
Bpu10I CCTNAGC 1 cut(s) 1341
BpuMI CCSGG 1 cut(s) 993
Bsa29I ATCGAT 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 791
BsaJI CCNNGG 3 cut(s) 80, 1038, 1259
BsaWI WCCGGW 2 cut(s) 65, 773
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 404, 571, 998, 1186
Bse118I RCCGGY 3 cut(s) 65, 282, 773
Bse1I ACTGG 7 cut(s) 380, 487, 595, 647, 837, 1071, 1389
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 791
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 3 cut(s) 80, 1038, 1259
BseGI GGATG 6 cut(s) 40, 103, 340, 721, 814, 1048
BseJI GATNNNNATC 1 cut(s) 791
BseLI CCNNNNNNNGG 5 cut(s) 282, 404, 571, 998, 1186
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 381, 1332
BseNI ACTGG 7 cut(s) 380, 487, 595, 647, 837, 1071, 1389
BseRI GAGGAG 3 cut(s) 19, 22, 684
BseSI GKGCMC 2 cut(s) 459, 1117
BseXI GCAGC 3 cut(s) 73, 525, 669
BsgI GTGCAG 1 cut(s) 906
Bsh1236I CGCG 1 cut(s) 858
BshFI GGCC 5 cut(s) 260, 282, 379, 562, 1179
BshTI ACCGGT 2 cut(s) 65, 773
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 4 cut(s) 66, 283, 774, 992
BslFI GGGAC 3 cut(s) 257, 819, 1240
BslI CCNNNNNNNGG 5 cut(s) 282, 404, 571, 998, 1186
BsmFI GGGAC 3 cut(s) 257, 819, 1240
BsmI GAATGC 2 cut(s) 138, 223
BsnI GGCC 5 cut(s) 260, 282, 379, 562, 1179
BsoBI CYCGRG 1 cut(s) 133
Bsp1286I GDGCHC 3 cut(s) 73, 459, 1117
Bsp143I GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
Bsp19I CCATGG 2 cut(s) 80, 1259
BspACI CCGC 2 cut(s) 276, 858
BspANI GGCC 5 cut(s) 260, 282, 379, 562, 1179
BspCNI CTCAG 2 cut(s) 380, 1333
BspDI ATCGAT 1 cut(s) 723
BspFNI CGCG 1 cut(s) 858
BspLI GGNNCC 2 cut(s) 259, 563
BspMAI CTGCAG 2 cut(s) 659, 1177
BspPI GGATC 3 cut(s) 364, 383, 719
BspQI GCTCTTC 1 cut(s) 1276
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 3 cut(s) 65, 282, 773
BsrI ACTGG 7 cut(s) 380, 487, 595, 647, 837, 1071, 1389
BssAI RCCGGY 3 cut(s) 65, 282, 773
BssECI CCNNGG 3 cut(s) 80, 1038, 1259
BssMI GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
BssNAI GTATAC 1 cut(s) 652
BssT1I CCWWGG 3 cut(s) 80, 1038, 1259
Bst1107I GTATAC 1 cut(s) 652
Bst4CI ACNGT 2 cut(s) 152, 761
Bst6I CTCTTC 2 cut(s) 739, 1276
BstC8I GCNNGC 2 cut(s) 280, 1177
BstDEI CTNAG 2 cut(s) 367, 1341
BstDSI CCRYGG 2 cut(s) 80, 1259
BstENI CCTNNNNNAGG 1 cut(s) 1184
BstF5I GGATG 6 cut(s) 40, 103, 340, 721, 814, 1048
BstFNI CGCG 1 cut(s) 858
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 7 cut(s) 14, 359, 391, 727, 784, 789, 1375
BstMBI GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
BstSCI CCNGG 1 cut(s) 991
BstSFI CTRYAG 2 cut(s) 655, 1173
BstSLI GKGCMC 2 cut(s) 459, 1117
BstUI CGCG 1 cut(s) 858
BstV1I GCAGC 3 cut(s) 73, 525, 669
BstV2I GAAGAC 1 cut(s) 762
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BstZ17I GTATAC 1 cut(s) 652
Bsu15I ATCGAT 1 cut(s) 723
BsuRI GGCC 5 cut(s) 260, 282, 379, 562, 1179
BsuTUI ATCGAT 1 cut(s) 723
BtgI CCRYGG 2 cut(s) 80, 1259
BtsCI GGATG 6 cut(s) 40, 103, 340, 721, 814, 1048
BtsIMutI CAGTG 3 cut(s) 157, 162, 1396
Cac8I GCNNGC 2 cut(s) 280, 1177
CaiI CAGNNNCTG 1 cut(s) 1321
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 3 cut(s) 65, 282, 773
Cfr13I GGNCC 3 cut(s) 258, 561, 636
ClaI ATCGAT 1 cut(s) 723
Csp6I GTAC 1 cut(s) 47
CspAI ACCGGT 2 cut(s) 65, 773
CviAII CATG 5 cut(s) 81, 662, 935, 1260, 1280
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 2 cut(s) 367, 1341
DpnI GATC 7 cut(s) 13, 358, 390, 726, 783, 788, 1374
DpnII GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
DraI TTTAAA 1 cut(s) 1144
EaeI YGGCCR 2 cut(s) 280, 377
Eam1104I CTCTTC 2 cut(s) 739, 1276
EarI CTCTTC 2 cut(s) 739, 1276
Eco130I CCWWGG 3 cut(s) 80, 1038, 1259
Eco147I AGGCCT 1 cut(s) 1179
Eco47I GGWCC 1 cut(s) 636
Eco57I CTGAAG 1 cut(s) 1343
Eco88I CYCGRG 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 1184
EcoO109I RGGNCCY 1 cut(s) 561
EcoRI GAATTC 1 cut(s) 526
EcoT14I CCWWGG 3 cut(s) 80, 1038, 1259
ErhI CCWWGG 3 cut(s) 80, 1038, 1259
FaeI CATG 5 cut(s) 84, 665, 938, 1263, 1283
FaqI GGGAC 3 cut(s) 257, 819, 1240
FatI CATG 5 cut(s) 80, 661, 934, 1259, 1279
FauI CCCGC 2 cut(s) 283, 851
FauNDI CATATG 2 cut(s) 403, 826
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 3 cut(s) 87, 514, 658
FokI GGATG 6 cut(s) 47, 90, 347, 728, 821, 1055
Fsp4HI GCNGC 3 cut(s) 87, 514, 658
FspBI CTAG 1 cut(s) 1184
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 87, 514, 658
GsuI CTGGAG 2 cut(s) 936, 1287
HaeIII GGCC 5 cut(s) 260, 282, 379, 562, 1179
HapII CCGG 4 cut(s) 66, 283, 774, 992
HhaI GCGC 1 cut(s) 176
Hin1II CATG 5 cut(s) 84, 665, 938, 1263, 1283
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HindIII AAGCTT 2 cut(s) 263, 298
HinfI GANTC 2 cut(s) 676, 731
HpaII CCGG 4 cut(s) 66, 283, 774, 992
HphI GGTGA 8 cut(s) 55, 148, 194, 618, 635, 696, 788, 1060
Hpy166II GTNNAC 4 cut(s) 63, 291, 652, 1068
Hpy188III TCNNGA 4 cut(s) 673, 728, 915, 1304
Hpy8I GTNNAC 4 cut(s) 63, 291, 652, 1068
HpyAV CCTTC 4 cut(s) 408, 990, 1190, 1318
HpyCH4III ACNGT 2 cut(s) 152, 761
HpyCH4V TGCA 9 cut(s) 56, 114, 344, 657, 750, 887, 938, 1175, 1394
HpyF3I CTNAG 2 cut(s) 367, 1341
Hsp92II CATG 5 cut(s) 84, 665, 938, 1263, 1283
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
LguI GCTCTTC 1 cut(s) 1276
LmnI GCTCC 2 cut(s) 76, 697
Lsp1109I GCAGC 3 cut(s) 73, 525, 669
LweI GCATC 2 cut(s) 331, 729
MaeI CTAG 1 cut(s) 1184
MaeIII GTNAC 6 cut(s) 154, 190, 536, 1073, 1243, 1358
MalI GATC 7 cut(s) 13, 358, 390, 726, 783, 788, 1374
MboI GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
MboII GAAGA 6 cut(s) 6, 217, 350, 726, 767, 1263
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 3 cut(s) 73, 459, 1117
MlsI TGGCCA 1 cut(s) 379
MluCI AATT 9 cut(s) 119, 167, 460, 526, 550, 622, 700, 891, 897
MluNI TGGCCA 1 cut(s) 379
MlyI GAGTC 1 cut(s) 725
MmeI TCCRAC 1 cut(s) 312
Mox20I TGGCCA 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 530
MscI TGGCCA 1 cut(s) 379
MseI TTAA 3 cut(s) 122, 620, 1143
MslI CAYNNNNRTG 1 cut(s) 1092
Msp20I TGGCCA 1 cut(s) 379
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 4 cut(s) 66, 283, 774, 992
MspR9I CCNGG 1 cut(s) 993
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 2 cut(s) 138, 223
MvnI CGCG 1 cut(s) 858
NciI CCSGG 1 cut(s) 993
NcoI CCATGG 2 cut(s) 80, 1259
NdeI CATATG 2 cut(s) 403, 826
NdeII GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
NlaIII CATG 5 cut(s) 84, 665, 938, 1263, 1283
NlaIV GGNNCC 2 cut(s) 259, 563
NmuCI GTSAC 3 cut(s) 154, 190, 1073
PceI AGGCCT 1 cut(s) 1179
PciSI GCTCTTC 1 cut(s) 1276
PctI GAATGC 2 cut(s) 138, 223
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 1 cut(s) 676
PflMI CCANNNNNTGG 2 cut(s) 404, 571
PfoI TCCNGGA 1 cut(s) 991
PinAI ACCGGT 2 cut(s) 65, 773
PkrI GCNGC 3 cut(s) 88, 515, 659
PleI GAGTC 1 cut(s) 725
PpsI GAGTC 1 cut(s) 725
PspN4I GGNNCC 2 cut(s) 259, 563
PspPI GGNCC 3 cut(s) 258, 561, 636
PstI CTGCAG 2 cut(s) 659, 1177
PstNI CAGNNNCTG 1 cut(s) 1321
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 1092
SapI GCTCTTC 1 cut(s) 1276
SaqAI TTAA 3 cut(s) 122, 620, 1143
SatI GCNGC 3 cut(s) 87, 514, 658
Sau3AI GATC 7 cut(s) 11, 356, 388, 724, 781, 786, 1372
Sau96I GGNCC 3 cut(s) 258, 561, 636
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 1 cut(s) 993
SduI GDGCHC 3 cut(s) 73, 459, 1117
SfaNI GCATC 2 cut(s) 331, 729
SfcI CTRYAG 2 cut(s) 655, 1173
SgrAI CRCCGGYG 2 cut(s) 65, 773
SinI GGWCC 1 cut(s) 636
SmiMI CAYNNNNRTG 1 cut(s) 1092
Sse9I AATT 9 cut(s) 119, 167, 460, 526, 550, 622, 700, 891, 897
SseBI AGGCCT 1 cut(s) 1179
SsiI CCGC 2 cut(s) 276, 858
SspI AATATT 1 cut(s) 1231
SspMI CTAG 1 cut(s) 1184
StuI AGGCCT 1 cut(s) 1179
StyD4I CCNGG 1 cut(s) 991
StyI CCWWGG 3 cut(s) 80, 1038, 1259
TaaI ACNGT 2 cut(s) 152, 761
TaqI TCGA 3 cut(s) 142, 409, 723
TaqII GACCGA 1 cut(s) 482
TasI AATT 9 cut(s) 119, 167, 460, 526, 550, 622, 700, 891, 897
TfiI GAWTC 1 cut(s) 676
Tru1I TTAA 3 cut(s) 122, 620, 1143
Tru9I TTAA 3 cut(s) 122, 620, 1143
TscAI CASTG 3 cut(s) 157, 169, 1396
TseFI GTSAC 3 cut(s) 154, 190, 1073
TseI GCWGC 3 cut(s) 86, 513, 657
Tsp45I GTSAC 3 cut(s) 154, 190, 1073
TspDTI ATGAA 6 cut(s) 351, 377, 596, 813, 923, 1268
TspGWI ACGGA 4 cut(s) 63, 524, 658, 728
TspRI CASTG 3 cut(s) 157, 169, 1396
Van91I CCANNNNNTGG 2 cut(s) 404, 571
VpaK11BI GGWCC 1 cut(s) 636
XagI CCTNNNNNAGG 1 cut(s) 1184
XapI RAATTY 4 cut(s) 167, 526, 622, 891
XmiI GTMKAC 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.